Dissection of synaptic excitability phenotypes by using a dominant-negative Shaker K+ channel subunit.

abstract：:Five instances of association between distinctive stromatolites and blue-green algal nannofossils are recorded from a 100-m sequence of carbonate rocks about 1.6 x 10(9) years old, along the south side of Paradise Creek, northwestern Queensland, Australia. No eukaryotes were identified in any of these systematically l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Licari GR,Cloud P

更新日期：1972-09-01 00:00:00

abstract：:In eukaryotes, transfer RNAs (tRNAs) are transcribed in the nucleus yet function in the cytoplasm; thus, tRNA movement within the cell was believed to be unidirectional--from the nucleus to the cytoplasm. It is now known that mature tRNAs also move in a retrograde direction from the cytoplasm to the nucleus via retrog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kramer EB,Hopper AK

更新日期：2013-12-24 00:00:00

abstract：:Determining trend and implementing detrending operations are important steps in data analysis. Yet there is no precise definition of "trend" nor any logical algorithm for extracting it. As a result, various ad hoc extrinsic methods have been used to determine trend and to facilitate a detrending operation. In this art...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu Z,Huang NE,Long SR,Peng CK

更新日期：2007-09-18 00:00:00

abstract：:A sensitive and rapid in situ method was developed to visualize sites of single-stranded (ss) DNA in cultured cells and in experimental test animals. Anti-bromodeoxyuridine antibody recognizes the halogenated base analog incorporated into chromosomal DNA only when substituted DNA is in the single strand form. After tr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raderschall E,Golub EI,Haaf T

更新日期：1999-03-02 00:00:00

abstract：:Ab initio multi-reference second-order perturbation theory computations are used to explore the photochemical behavior of two ion pairs constituted by a chloride counterion interacting with either a rhodopsin or bacteriorhodopsin chromophore model (i.e., the 4-cis-gamma-methylnona-2,4,6,8-tetraeniminium and all-trans-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cembran A,Bernardi F,Olivucci M,Garavelli M

更新日期：2005-05-03 00:00:00

abstract：:The activity of a number of proteins is regulated by self-processing reactions. Elegant examples are the cleavage of the prokaryotic LexA and lambdaCI transcriptional repressors and the UmuD-like mutagenesis proteins. Various studies support the hypothesis that LexA and lambdaCI cleavage reactions are predominantly in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McDonald JP,Frank EG,Levine AS,Woodgate R

更新日期：1998-02-17 00:00:00

abstract：:A specific lipoprotein of the E. coli outer membrane has been synthesized in a cell-free system directed by purified messenger RNA. The mRNA for the lipoprotein was purified as 7S RNA about 250-fold from exponentially growing cells. Protein synthesis of the cell-free system was totally dependent upon the addition of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hirashima A,Wang S,Inouye M

更新日期：1974-10-01 00:00:00

abstract：:In order to study the mutagenic effects of site-specific, covalent modifications of biologically active DNA, we need host cells that are permissive for any type of mutation that might be produced in vivo from the modified DNA. Specifically, we require a general, in vivo complementation system for the bacteriophage phi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Humayun MZ,Chambers RW

更新日期：1978-02-01 00:00:00

abstract：:The intermediates and the rate-limiting step in the crystallization of deoxygenated sickle hemoglobin have been determined by a kinetic study with the use of electron microscopy. In slowly stirred solutions of deoxygenated hemoglobin S [Pumphrey, J. & Steinhardt, J. (1977) J. Mol. Biol. 112, 359--375], the sequential ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wilson SM,Makinen MW

更新日期：1980-02-01 00:00:00

abstract：:Phospholipid flippases (P4-ATPases) utilize ATP to translocate specific phospholipids from the exoplasmic leaflet to the cytoplasmic leaflet of biological membranes, thus generating and maintaining transmembrane lipid asymmetry essential for a variety of cellular processes. P4-ATPases belong to the P-type ATPase prote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tadini-Buoninsegni F,Mikkelsen SA,Mogensen LS,Molday RS,Andersen JP

更新日期：2019-08-13 00:00:00

abstract：:A method is proposed for rapidly, quantitatively comparing protein structures of arbitrary sizes, based on the differential geometric representation. The method is applied to a group of 22 protein x-ray structures, and the resulting network of closest relationships is delineated. Several well-known fold types are auto...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rackovsky S,Goldstein DA

更新日期：1988-02-01 00:00:00

abstract：:The slow rolling motion of leukocytes along the walls of blood vessels mediated by specific receptor-ligand adhesion is important in inflammation and occurs in postcapillary venules over a wide range of wall shear stresses and vessel diameters. The ability of hydrodynamic collisions between cells to induce capture of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： King MR,Hammer DA

更新日期：2001-12-18 00:00:00

abstract：:Viperin is an interferon (IFN)-inducible multifunctional protein. Recent evidence from high-throughput analyses indicates that most IFN-inducible proteins, including viperin, are intrinsically expressed in specific tissues; however, the respective intrinsic functions are unknown. Here we show that the intrinsic expres...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eom J,Kim JJ,Yoon SG,Jeong H,Son S,Lee JB,Yoo J,Seo HJ,Cho Y,Kim KS,Choi KM,Kim IY,Lee HY,Nam KT,Cresswell P,Seong JK,Seo JY

更新日期：2019-08-27 00:00:00

abstract：:A TL+ leukemia of a (B6 X A)F1 hybrid mouse (H-2b/H-2a) was previously subjected to immunoselection against H-2a by passage in (B6 X A.SW)F1 mice (H-2b/H-2s). A variant leukemia line was obtained that serologically lacked not only the H-2a phenotype but also the TL phenotype determined by the linked cis Tlaa allele of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shen FW,Chaganti RS,Doucette LA,Litman GW,Steinmetz M,Hood L,Boyse EA

更新日期：1984-10-01 00:00:00

abstract：:The influenza virus genes that confer efficient transmission of epidemic and pandemic strains in humans have not been identified. The rapid spread and severe disease caused by the 1918 influenza pandemic virus makes it an ideal virus to study the transmissibility of potentially pandemic influenza strains. Here, we use...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Van Hoeven N,Pappas C,Belser JA,Maines TR,Zeng H,García-Sastre A,Sasisekharan R,Katz JM,Tumpey TM

更新日期：2009-03-03 00:00:00

abstract：:Phosphatidylinositol 3 (PI3)-kinase enhancer (PIKE) is a nuclear GTPase that enhances PI3-kinase activity in a GTP-dependent manner. Both PIKE-L and -A isoforms contain GTPase, pleckstrin homology (PH), ADP ribosylation factor-GTPase-activating protein, and two ankyrin repeats domains, and C-terminal ADP ribosylation ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu Y,Liu Z,Ye K

更新日期：2005-11-15 00:00:00

abstract：:Cigarette smoking causes emphysema, a fatal disease involving extensive structural and functional damage of the lung. Using a guinea pig model and human lung cells, we show that oxidant(s) present in tobacco smoke not only cause direct oxidative damage of lung proteins, contributing to the major share of lung injury, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gupta I,Ganguly S,Rozanas CR,Stuehr DJ,Panda K

更新日期：2016-07-19 00:00:00

abstract：:Polyadenylation, the process of eukaryotic mRNA 3' end formation, is essential for gene expression and cell viability. Polyadenylation of male germ cell mRNAs is unusual, exhibiting increased alternative polyadenylation, decreased AAUAAA polyadenylation signal use, and reduced downstream sequence element dependence. C...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dass B,Tardif S,Park JY,Tian B,Weitlauf HM,Hess RA,Carnes K,Griswold MD,Small CL,Macdonald CC

更新日期：2007-12-18 00:00:00

abstract：:Experimental lemurs either were infected orally with the agent of bovine spongiform encephalopathy (BSE) or were maintained as uninfected control animals. Immunohistochemical examination for proteinase-resistant protein (prion protein or PrP) was performed on tissues from two infected but still asymptomatic lemurs, ki...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bons N,Mestre-Frances N,Belli P,Cathala F,Gajdusek DC,Brown P

更新日期：1999-03-30 00:00:00

abstract：:To assess the contribution to immunoglobulin heavy chain diversity made by recombination between variable region (VH) genes and joining region (JH) genes, we have determined the sequence of about 2000 nucleotides spanning the rearranged JH gene cluster associated with the VH gene expressed in plasmacytoma HPC76. The a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gough NM,Bernard O

更新日期：1981-01-01 00:00:00

abstract：:Identifying the downstream targets of microRNAs (miRNAs) is essential to understanding cellular regulatory networks. We devised a direct biochemical method for miRNA target discovery that combined RNA-induced silencing complex (RISC) purification with microarray analysis of bound mRNAs. Because targets of miR-124a hav...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karginov FV,Conaco C,Xuan Z,Schmidt BH,Parker JS,Mandel G,Hannon GJ

更新日期：2007-12-04 00:00:00

abstract：:T-helper 17 (Th17) cells have important functions in adaptor immunity and have also been implicated in inflammatory disorders. The bromodomain and extraterminal domain (BET) family proteins regulate gene transcription during lineage-specific differentiation of naïve CD4+ T cells to produce mature T-helper cells. Inhib...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheung K,Lu G,Sharma R,Vincek A,Zhang R,Plotnikov AN,Zhang F,Zhang Q,Ju Y,Hu Y,Zhao L,Han X,Meslamani J,Xu F,Jaganathan A,Shen T,Zhu H,Rusinova E,Zeng L,Zhou J,Yang J,Peng L,Ohlmeyer M,Walsh MJ,Zhang DY,

更新日期：2017-03-14 00:00:00

abstract：:Xenogeneic monoclonal antibodies were prepared to the murine interleukin 2 (IL-2)-dependent HT2 cell line. One rat IgM monoclonal antibody (7D4) was identified that inhibited proliferation of the HT2 cells and of IL-2-dependent CTLL cells in the presence of crude rat IL-2 as well as of purified human IL-2. The level o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Malek TR,Robb RJ,Shevach EM

更新日期：1983-09-01 00:00:00

abstract：:Self-assembled monolayer-protected nanoparticles are promising candidates for applications, such as sensing and drug delivery, in which the molecular ligands' interactions with the surrounding environment play a crucial role. We recently showed that, when gold nanoparticles are coated with a binary mixture of immiscib...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Centrone A,Penzo E,Sharma M,Myerson JW,Jackson AM,Marzari N,Stellacci F

更新日期：2008-07-22 00:00:00

abstract：:The thin layer of airway surface liquid (ASL) contains antimicrobial substances that kill the small numbers of bacteria that are constantly being deposited in the lungs. An increase in ASL salt concentration inhibits the activity of airway antimicrobial factors and may partially explain the pathogenesis of cystic fibr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zabner J,Seiler MP,Launspach JL,Karp PH,Kearney WR,Look DC,Smith JJ,Welsh MJ

更新日期：2000-10-10 00:00:00

abstract：:In the last 60,000 y humans have expanded across the globe and now occupy a wider range than any other terrestrial species. Our ability to successfully adapt to such a diverse range of habitats is often explained in terms of our cognitive ability. Humans have relatively bigger brains and more computing power than othe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boyd R,Richerson PJ,Henrich J

更新日期：2011-06-28 00:00:00

abstract：:We described previously a mutation in maize, hcf106, caused by the insertion of a Mu1 transposon. When the Mu transposon system is in an active phase, hcf106 conditions a nonphotosynthetic, pale green phenotype. However, when the Mu system is inactive (a state correlated with hypermethylation of Mu elements), the plan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barkan A,Martienssen RA

更新日期：1991-04-15 00:00:00

abstract：:Unconscious neural activity has been repeatedly shown to precede and potentially even influence subsequent free decisions. However, to date, such findings have been mostly restricted to simple motor choices, and despite considerable debate, there is no evidence that the outcome of more complex free decisions can be pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Soon CS,He AH,Bode S,Haynes JD

更新日期：2013-04-09 00:00:00

abstract：:Memory CD8 T cells provide durable protection against diverse intracellular pathogens and can be broadly segregated into distinct circulating and tissue-resident populations. Paradigmatic studies have demonstrated that circulating memory cells can be further divided into effector memory (Tem) and central memory (Tcm) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Milner JJ,Nguyen H,Omilusik K,Reina-Campos M,Tsai M,Toma C,Delpoux A,Boland BS,Hedrick SM,Chang JT,Goldrath AW

更新日期：2020-10-13 00:00:00

abstract：:The fluorescence of the supposedly buried tryptophan in ribonuclease T1 has been found to be collisionally quenched by acrylamide with a rate constant of 3 X 10(8) M--1 sec--1. Only a slight decrease in the quenching rate is observed upon a 5-fold increase in the viscosity of the solution. For this to be the case, the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eftink MR,Ghiron CA

更新日期：1975-09-01 00:00:00