Abstract:
:The attachment of human polymorphonuclear leukocytes (PMN) to bovine endothelial monolayers is increased by pretreatment of the endothelial cells with leukotriene B4 (LTB4). The response is dose dependent with a maximum at 10(-6) M, requires no more than 1 min of preincubation for the maximal effect, and decays linearly over 30 min once the LTB4 is removed. Preincubation of PMN with LTB4 does not augment subsequent adhesion to a plastic substratum or an untreated monolayer of endothelial cells and eliminates the augmented attachment to LTB4-pretreated endothelial cells. The simultaneous exposure of both cell types during the adhesion interaction gives a biphasic dose-related increment, suggesting that the inhibition of the neutrophil adherence is less marked than with a pretreatment interval. Thus, the LTB4-dependent adherence of PMN to endothelial cells is selectively mediated by endothelial cells, is dose related, is reversible upon removal of the agonist from the endothelial cells, and is opposed by the direct action of LTB4 on the PMN.
journal_name
Proc Natl Acad Sci U S Aauthors
Hoover RL,Karnovsky MJ,Austen KF,Corey EJ,Lewis RAdoi
10.1073/pnas.81.7.2191subject
Has Abstractpub_date
1984-04-01 00:00:00pages
2191-3issue
7eissn
0027-8424issn
1091-6490journal_volume
81pub_type
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:1987-04-01 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2002-02-19 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:1992-10-01 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:1982-12-01 00:00:00
abstract::The spectra of the alpha, beta, and gamma forms of zinc monoarsanilazotyrosine-248 carboxypeptidase A are indistinguishable. At pH 8.2 their crystals are yellow, while their solutions are red, lambda(max) 510 nm. Absorption and circular dichroism-pH titrations of the modified zinc and apoenzymes demonstrate that the a...
journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:1973-07-01 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:1993-04-01 00:00:00
abstract::The Ritz upper bound to eigenvalues of Hermitian operators is essential for many applications in science. It is a staple of quantum chemistry and physics computations. The lower bound devised by Temple in 1928 [G. Temple, Proc. R. Soc. A Math. Phys. Eng. Sci. 119, 276-293 (1928)] is not, since it converges too slowly....
journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2020-07-14 00:00:00