Abstract:
:The mouse sperm receptor, a glycoprotein called ZP3, is synthesized and secreted by growing oocytes. It is present in more than a billion copies in the unfertilized egg's extracellular coat, or zona pellucida. We have cloned and characterized a region of the mouse (CD-1) genome that spans 10 kilobases of the ZP3 locus. The genomic clones described encompass the entire ZP3 coding region, which contains eight exons. The exons were identified, mapped, and sequenced, yielding the entire primary structure of the ZP3 polypeptide chain (424 amino acids; Mr, 46,300), which includes a 22-amino acid signal sequence. In addition, sequencing of genomic clones has revealed some unusual features of ZP3 mRNA and a region just downstream of the ZP3 gene.
journal_name
Proc Natl Acad Sci U S Aauthors
Kinloch RA,Roller RJ,Fimiani CM,Wassarman DA,Wassarman PMdoi
10.1073/pnas.85.17.6409subject
Has Abstractpub_date
1988-09-01 00:00:00pages
6409-13issue
17eissn
0027-8424issn
1091-6490journal_volume
85pub_type
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