Abstract:
:The karyophilic properties of the HIV-1 nucleoprotein complex facilitate infection of nondividing cells such as macrophages and quiescent T lymphocytes, and allow the in vivo delivery of transgenes by HIV-derived retroviral vectors into terminally differentiated cells such as neurons. Although the viral matrix (MA) and Vpr proteins have previously been shown to play important roles in this process, we demonstrate here that integrase, the enzyme responsible for mediating the integration of the viral genome in the host cell chromosome, can suffice to connect the HIV-1 preintegration complex with the cell nuclear import machinery. This novel function of integrase reflects the recognition of an atypical bipartite nuclear localization signal by the importin/karyopherin pathway.
journal_name
Proc Natl Acad Sci U S Aauthors
Gallay P,Hope T,Chin D,Trono Ddoi
10.1073/pnas.94.18.9825subject
Has Abstractpub_date
1997-09-02 00:00:00pages
9825-30issue
18eissn
0027-8424issn
1091-6490journal_volume
94pub_type
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