Role of guanine nucleotides in protein synthesis. Elongation factor G and guanosine 5'-triphosphate,3'-diphosphate.

abstract：:Idiopathic pulmonary fibrosis (IPF)/usual interstitial pneumonia is a ravaging condition of progressive lung scarring and destruction. Anti-inflammatory therapies including corticosteroids have limited efficacy in this ultimately fatal disorder. An important unmet need is to identify new agents that interact with key ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Henderson WR Jr,Chi EY,Ye X,Nguyen C,Tien YT,Zhou B,Borok Z,Knight DA,Kahn M

更新日期：2010-08-10 00:00:00

abstract：:Sponges (Porifera), the simplest and earliest multicellular organisms, are thought to have evolved from their unicellular ancestors about 1 billion years ago by developing cell-recognition and adhesion mechanisms to discriminate against "non-self." Consequently, they are used as models for investigating recognition ph...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haseley SR,Vermeer HJ,Kamerling JP,Vliegenthart JF

更新日期：2001-07-31 00:00:00

abstract：:The most common monogenic cause of small-vessel disease leading to ischemic stroke and vascular dementia is the neurodegenerative syndrome cerebral autosomal-dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL), which is associated with mutations in the Notch 3 receptor. CADASIL pathology ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arboleda-Velasquez JF,Manent J,Lee JH,Tikka S,Ospina C,Vanderburg CR,Frosch MP,Rodríguez-Falcón M,Villen J,Gygi S,Lopera F,Kalimo H,Moskowitz MA,Ayata C,Louvi A,Artavanis-Tsakonas S

更新日期：2011-05-24 00:00:00

abstract：:We show that a drop of liquid a few hundred microns in diameter placed under a solid, elastic, thin film (∼10 μm thick) causes it to bulge by tens of microns. The deformed shape is governed by equilibrium of tensions exerted by the various interfaces and the solid film, a form of Neumann's triangle. Unlike Young's equ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nadermann N,Hui CY,Jagota A

更新日期：2013-06-25 00:00:00

abstract：:In this study we examine how the introduction of a reference lottery with nonrandom outcomes alters the way in which choices among pairs of lotteries are made, even if it does not alter the choices. We use different domains (some of the lotteries produce gains, other losses) and different contexts (one member of the p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dickhaut J,McCabe K,Nagode JC,Rustichini A,Smith K,Pardo JV

更新日期：2003-03-18 00:00:00

abstract：:Under steady-state conditions, the immune system is poised to sense and respond to the microbiota. As such, immunity to the microbiota, including T cell responses, is expected to precede any inflammatory trigger. How this pool of preformed microbiota-specific T cells contributes to tissue pathologies remains unclear. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hurabielle C,Link VM,Bouladoux N,Han SJ,Merrill ED,Lightfoot YL,Seto N,Bleck CKE,Smelkinson M,Harrison OJ,Linehan JL,Tamoutounour S,Lionakis MS,Kaplan MJ,Nakajima S,Belkaid Y

更新日期：2020-07-14 00:00:00

abstract：:Activation of the c-abl protooncogene occurs in Abelson murine leukemia virus, in Hardy-Zuckerman 2 feline sarcoma virus, and during the chromosomal translocations that generate BCR-ABL gene fusion products. To study the molecular mechanism involved in the c-abl activation, we have created a series of modifications in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shore SK,Bogart SL,Reddy EP

更新日期：1990-09-01 00:00:00

abstract：:Chromosome 14 was transferred into tumorigenic nasopharyngeal carcinoma and esophageal carcinoma cell lines by a microcell-mediated chromosome transfer approach. Functional complementation of defects present in the cancer cells suppressed tumor formation. A candidate tumor-suppressor gene, cysteine-rich intestinal pro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheung AK,Ko JM,Lung HL,Chan KW,Stanbridge EJ,Zabarovsky E,Tokino T,Kashima L,Suzuki T,Kwong DL,Chua D,Tsao SW,Lung ML

更新日期：2011-05-17 00:00:00

abstract：:Posttranscriptional regulation is an important step in the regulation of gene expression. In this article, we show an unexpected connection between two proteins that participate in different processes of posttranscriptional regulation that ensures the production of functional mRNA molecules. Specifically, we show that...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Agranat L,Raitskin O,Sperling J,Sperling R

更新日期：2008-04-01 00:00:00

abstract：:We present a unique proxy for the reconstruction of variation in precipitation over the Amazon: oxygen isotope ratios in annual rings in tropical cedar (Cedrela odorata). A century-long record from northern Bolivia shows that tree rings preserve the signal of oxygen isotopes in precipitation during the wet season, wit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brienen RJ,Helle G,Pons TL,Guyot JL,Gloor M

更新日期：2012-10-16 00:00:00

abstract：:The conditional lifetime expectancy function (LEF) is the expected lifetime of a subject given survival past a certain time point and the values of a set of explanatory variables. This function is attractive to researchers because it summarizes the entire residual life distribution and has an easy interpretation compa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kong J,Klein BE,Klein R,Wahba G

更新日期：2015-09-29 00:00:00

abstract：:Fluoroquinolones are antibacterial agents that attack DNA gyrase and topoisomerase IV on chromosomal DNA. The existence of two fluoroquinolone targets and stepwise accumulation of resistance suggested that new quinolones could be found that would require cells to obtain two topoisomerase mutations to display resistanc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao X,Xu C,Domagala J,Drlica K

更新日期：1997-12-09 00:00:00

abstract：:To study the permanency of response to selection for a quantitative character in finite populations and the nature of the genetic effects that contribute to this response, we have used the covariance between ancestors and descendents within populations. Effects and variances are defined for an initial equilibrium rand...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cockerham CC,Tachida H

更新日期：1988-03-01 00:00:00

abstract：:Recent archaeological discoveries have revealed that pigment use, beads, engravings, and sophisticated stone and bone tools were already present in southern Africa 75,000 y ago. Many of these artifacts disappeared by 60,000 y ago, suggesting that modern behavior appeared in the past and was subsequently lost before be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： d'Errico F,Backwell L,Villa P,Degano I,Lucejko JJ,Bamford MK,Higham TF,Colombini MP,Beaumont PB

更新日期：2012-08-14 00:00:00

abstract：:This work argues that, in a polarized environment, scientists can minimize the likelihood that the audience's biased processing will lead to rejection of their message if they not only eschew advocacy but also, convey that they are sharers of knowledge faithful to science's way of knowing and respectful of the audienc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jamieson KH,Hardy BW

更新日期：2014-09-16 00:00:00

abstract：:The persistent tetrodotoxin (TTX)-sensitive sodium current, detected in neurons of many regions of mammalian brains, is associated with many essential neuronal activities, including boosting of excitatory synaptic inputs, acceleration of firing rates, and promotion of oscillatory neuronal activities. However, the orig...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu Z,Song W,Dong K

更新日期：2004-08-10 00:00:00

abstract：:The amino acid sequence for chicken smooth muscle myosin light chain kinase (smMLCK) was deduced from a full-length cDNA. This has allowed definition of both the complete sequence of the inactive 64-kDa proteolytic fragment, which contains the pseudosubstrate autoregulatory sequence, and of the active 61-kDa Ca2+/calm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Olson NJ,Pearson RB,Needleman DS,Hurwitz MY,Kemp BE,Means AR

更新日期：1990-03-01 00:00:00

abstract：:An antibody (anti-p21ser) was raised against a ras p21-related synthetic peptide and was able to recognize specifically the substitution of serine for glycine at amino acid 12 of p21. This substitution causes oncogenic activation of p21. Anti-p21ser was found to immunoprecipitate v-Ki-ras p21 and to strongly inhibit i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clark R,Wong G,Arnheim N,Nitecki D,McCormick F

更新日期：1985-08-01 00:00:00

abstract：:Ca2+-induced inhibition of alpha1C voltage-gated Ca2+ channels is a physiologically important regulatory mechanism that shortens the mean open time of these otherwise long-lasting high-voltage-activated channels. The mechanism of action of Ca2+ has been a matter of some controversy, as previous studies have proposed t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Qin N,Olcese R,Bransby M,Lin T,Birnbaumer L

更新日期：1999-03-02 00:00:00

abstract：:Mutations at the mouse limb deformity (ld) locus result in defects of growth and patterning of the limb and kidney during embryonic development. The gene responsible for this phenotype is large and complex, with the capacity to generate a number of alternatively spliced messenger RNA transcripts encoding nuclear prote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vogt TF,Jackson-Grusby L,Rush J,Leder P

更新日期：1993-06-15 00:00:00

abstract：:Pbx-regulating protein-1 (Prep1) is a tumor suppressor, whereas myeloid ecotropic viral integration site-1 (Meis1) is an oncogene. We show that, to perform these activities in mouse embryonic fibroblasts, both proteins competitively heterodimerize with pre-B-cell leukemia homeobox-1 (Pbx1). Meis1 alone transforms Prep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dardaei L,Longobardi E,Blasi F

更新日期：2014-03-11 00:00:00

abstract：:The retinoblastoma gene product (the RB protein) is phosphorylated in a cell cycle-dependent manner and this modification is believed to be important for cells to progress through the cell cycle. We found that purified cdk2 (cyclin-dependent kinase/cell division kinase 2) can phosphorylate the RB protein in vitro at t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Akiyama T,Ohuchi T,Sumida S,Matsumoto K,Toyoshima K

更新日期：1992-09-01 00:00:00

abstract：:A site that is preferentially cleaved by the single-strand-specific endonuclease from Aspergillus oryzae was located in vitro 180 base pairs upstream from the 5' end of the chicken pro-alpha 2(I) collagen gene. It is found in supercoiled plasmids with a negative superhelical density of -0.024 or more but not in linear...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Finer MH,Fodor EJ,Boedtker H,Doty P

更新日期：1984-03-01 00:00:00

abstract：:A recombinant plasmid containing sequences complementary to human pro-alpha l(I) collagen mRNA was used for the chromosomal assignment of the pro-alpha l(I) collagen gene. Restriction endonuclease analysis of DNA from mouse-human and Chinese hamster-human somatic cell hybrids revealed cosegregation with human chromoso...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huerre C,Junien C,Weil D,Chu ML,Morabito M,Van Cong N,Myers JC,Foubert C,Gross MS,Prockop DJ,Boué A,Kaplan JC,de la Chapelle A,Ramirez F

更新日期：1982-11-01 00:00:00

abstract：:The Lyme disease spirochete Borrelia burgdorferi exists in nature in an enzootic cycle that involves the arthropod vector Ixodes scapularis and mammalian reservoirs. To disseminate within and between these hosts, spirochetes must migrate through complex, polymeric environments such as the basement membrane of the tick...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harman MW,Dunham-Ems SM,Caimano MJ,Belperron AA,Bockenstedt LK,Fu HC,Radolf JD,Wolgemuth CW

更新日期：2012-02-21 00:00:00

abstract：:The COVID-19 pandemic is caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and has spread worldwide, with millions of cases and more than 1 million deaths to date. The gravity of the situation mandates accelerated efforts to identify safe and effective vaccines. Here, we generated measles virus (M...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hörner C,Schürmann C,Auste A,Ebenig A,Muraleedharan S,Dinnon KH 3rd,Scholz T,Herrmann M,Schnierle BS,Baric RS,Mühlebach MD

更新日期：2020-12-22 00:00:00

abstract：:It was previously shown that coexpression of the lactose permease of Escherichia coli in two contiguous fragments leads to functional complementation. We demonstrate here that site-directed thiol crosslinking of coexpressed permease fragments can be used to determine helix proximity in situ without the necessity of pu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu J,Kaback HR

更新日期：1996-12-10 00:00:00

abstract：:During mitosis, the mammalian Golgi vesiculates and, upon partitioning, reassembles in each daughter cell; however, it is not clear whether the disassembly process per se is important for partitioning or is merely an outcome of mitotic entry. Here, we show that Golgi vesiculation is required for progression to metapha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guizzunti G,Seemann J

更新日期：2016-10-25 00:00:00

abstract：:A bone-resorbing factor, comparable to the osteoclast-activating factor (OAF) produced from peripheral blood leukocytes, is shown to be produced by murine spleen cells activated with the T-cell mitogen Con A. Murine OAF is demonstrated here as being a product of the interaction between thymus-derived T lymphocytes and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Horowitz M,Vignery A,Gershon RK,Baron R

更新日期：1984-04-01 00:00:00

abstract：:Pathways and enzymes of fatty acid synthase-mediated, long-even-chain (generally C16-C20) fatty acid synthesis are well studied, and general metabolism involved in short-chain (C4-C7) fatty acid biosynthesis is also understood. In contrast, mechanisms of medium-chain (C8-C14) fatty acid synthesis are unclear. Recent w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kroumova AB,Xie Z,Wagner GJ

更新日期：1994-11-22 00:00:00