Regulatory and structural motifs of chicken gizzard myosin light chain kinase.

abstract：:A 2.9-kilobase Acc I fragment of the DNA of the pneumococcal bacteriophage Cp-1, containing the cpl gene, hybridizes with the lytA gene encoding the pneumococcal amidase. The nucleotide sequence of the cpl gene of Cp-1, encoding a muramidase (CPL), has been determined. The 3' regions of the cpl and lytA coding sequenc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： García E,García JL,García P,Arrarás A,Sánchez-Puelles JM,López R

更新日期：1988-02-01 00:00:00

abstract：:There is great interest in understanding the mechanisms of expression underlying long-term potentiation (LTP). They are agreed to involve an increase in synaptic efficacy, which is described by three multiplicative parameters: p, the probability of neurotransmitter release; n, the number of active release sites; and q...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schulz PE

更新日期：1997-05-27 00:00:00

abstract：:Elucidation of the genetic control of rice architecture is crucial due to the global demand for high crop yields. Rice architecture is a complex trait affected by plant height, tillering, and panicle morphology. In this study, principal component analysis (PCA) on 8 typical traits related to plant architecture reveale...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yano K,Morinaka Y,Wang F,Huang P,Takehara S,Hirai T,Ito A,Koketsu E,Kawamura M,Kotake K,Yoshida S,Endo M,Tamiya G,Kitano H,Ueguchi-Tanaka M,Hirano K,Matsuoka M

更新日期：2019-10-15 00:00:00

abstract：:The role of chemotaxis in the virulence of gastrointestinal pathogens is ill defined. Counterintuitively, nonchemotactic mutants of the polarly flagellated pathogen Vibrio cholerae greatly out-compete the wild-type strain during infection of the small intestine. We show that the out-competition phenotype is dependent ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Butler SM,Camilli A

更新日期：2004-04-06 00:00:00

abstract：:Synaptic loss is the cardinal feature linking neuropathology to cognitive decline in Alzheimer's disease (AD). However, the mechanism of synaptic damage remains incompletely understood. Here, using FRET-based glutamate sensor imaging, we show that amyloid-β peptide (Aβ) engages α7 nicotinic acetylcholine receptors to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Talantova M,Sanz-Blasco S,Zhang X,Xia P,Akhtar MW,Okamoto S,Dziewczapolski G,Nakamura T,Cao G,Pratt AE,Kang YJ,Tu S,Molokanova E,McKercher SR,Hires SA,Sason H,Stouffer DG,Buczynski MW,Solomon JP,Michael S,Powers E

更新日期：2013-07-02 00:00:00

abstract：:Pyrimethamine (Pyr) targets dihydrofolate reductase of Plasmodium vivax (PvDHFR) as well as other malarial parasites, but its use as antimalarial is hampered by the widespread high resistance. Comparison of the crystal structures of PvDHFR from wild-type and the Pyr-resistant (SP21, Ser-58 --> Arg + Ser-117 --> Asn) s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kongsaeree P,Khongsuk P,Leartsakulpanich U,Chitnumsub P,Tarnchompoo B,Walkinshaw MD,Yuthavong Y

更新日期：2005-09-13 00:00:00

abstract：:T cells are activated physiologically by triggering the T-cell receptor-CD3 complex. There is evidence that invariant accessory molecules on the T-cell membrane (CD8 and CD4) are involved in the major histocompatibility complex-restricted recognition process. Moreover, binding and crosslinking of these accessory molec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Samstag Y,Emmrich F,Staehelin T

更新日期：1988-12-01 00:00:00

abstract：:We compared students' self-reported perception of learning with their actual learning under controlled conditions in large-enrollment introductory college physics courses taught using 1) active instruction (following best practices in the discipline) and 2) passive instruction (lectures by experienced and highly rated...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Deslauriers L,McCarty LS,Miller K,Callaghan K,Kestin G

更新日期：2019-09-24 00:00:00

abstract：:The bottom-up design of protein-based signaling networks is a key goal of synthetic biology; yet, it remains elusive due to our inability to tailor-make signal transducers and receptors that can be readily compiled into defined signaling networks. Here, we report a generic approach for the construction of protein-base...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stein V,Alexandrov K

更新日期：2014-11-11 00:00:00

abstract：:The study of photoinitiated electron transfer in donor-bridge-acceptor molecules has helped elucidate the distance dependence of electron transfer rates and behavior of various electron transfer mechanisms. In all reported cases, the energies of the bridge electronic states involved in the electron transfer change dra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldsmith RH,Sinks LE,Kelley RF,Betzen LJ,Liu W,Weiss EA,Ratner MA,Wasielewski MR

更新日期：2005-03-08 00:00:00

abstract：:We have used low magnesium concentrations and the specific antagonist D-2-amino-5-phosphonopentanoate (D-AP5) to estimate the effects of long-term potentiation (LTP) on the N-methyl-D-aspartate (NMDA) and non-NMDA receptor-mediated components of postsynaptic responses. LTP induction resulted in a considerably larger p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Muller D,Lynch G

更新日期：1988-12-01 00:00:00

abstract：:Poly(A)-containing HeLa cell mRNA prepared from cells labeled with [methyl-(3)H]methionine or [(32)P]phosphate was found to contain a variety of methylated, blocked 5'-terminal structures of two general types: m(7)GpppN(7)-Np and m(7)GpppN(m)-N(m)-Np. In addition, about one-third of the [(3)H]methyl label was present ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Furuichi Y,Morgan M,Shatkin AJ,Jelinek W,Salditt-Georgieff M,Darnell JE

更新日期：1975-05-01 00:00:00

abstract：:The three-dimensional structures of the inactive protein precursors (zymogens) of the serine, cysteine, aspartic, and metalloprotease classes of proteolytic enzymes are known. Comparisons of these structures with those of the mature, active proteases reveal that, in general, the preformed, active conformations of the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Khan AR,Khazanovich-Bernstein N,Bergmann EM,James MN

更新日期：1999-09-28 00:00:00

abstract：:A small number of facial expressions may be universal in that they are produced by the same basic affective states and recognized as such throughout the world. However, other aspects of emotionally expressive behavior vary widely across culture. Just why do they vary? We propose that some cultural differences in expre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rychlowska M,Miyamoto Y,Matsumoto D,Hess U,Gilboa-Schechtman E,Kamble S,Muluk H,Masuda T,Niedenthal PM

更新日期：2015-05-12 00:00:00

abstract：:The transcription factor Krüppel-like factor 2 (KLF2) is critical for normal trafficking of T lymphocytes, but its role in B cells is unclear. We report that B cell-specific KLF2 deficiency leads to decreased expression of the trafficking molecules CD62L and β7-integrin, yet expression of sphingosine-1 phosphate recep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hart GT,Wang X,Hogquist KA,Jameson SC

更新日期：2011-01-11 00:00:00

abstract：:Fluoride activation of adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] is significantly enhanced (2 to 5 times) by a protein factor isolated from rat brain. The fluoride-dependent adenylate cyclase stimulator (FCS) is nondialyzable, trypsin-labile, and stable at 90 degrees C for 10 min. FCS stimula...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rasenick MM,Bitensky MW

更新日期：1980-08-01 00:00:00

abstract：:Protein sequences are often highly redundant and evolution can change them beyond recognition. It can therefore be difficult to identify proteins with functional or structural similarities by inspection of their sequences. Here we have used an experimental evolutionary approach to detect hidden similarities between th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Christ D,Winter G

更新日期：2003-11-11 00:00:00

abstract：:The utility of adenovirus (Ad) vectors for gene transduction can be limited by receptor specificity. We developed a gene-delivery vehicle in which the potent Ad5 vector was genetically reengineered to display the mucosal-targeting sigma1 protein of reovirus type 3 Dearing (T3D). A sigma1 construct containing all but a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mercier GT,Campbell JA,Chappell JD,Stehle T,Dermody TS,Barry MA

更新日期：2004-04-20 00:00:00

abstract：:We have developed a reliable genetic selection strategy for isolating interacting proteins based on the "hitchhiker" mechanism of the Escherichia coli twin-arginine translocation (Tat) pathway. This method, designated FLI-TRAP (functional ligand-binding identification by Tat-based recognition of associating proteins),...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Waraho D,DeLisa MP

更新日期：2009-03-10 00:00:00

abstract：:Linkage disequilibrium between polymorphisms in a natural population may result from various evolutionary forces, including random genetic drift due to sampling of gametes during reproduction, restricted migration between subpopulations in a subdivided population, or epistatic selection. In this report, we present evi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kirby DA,Muse SV,Stephan W

更新日期：1995-09-26 00:00:00

abstract：:Inducible cAMP early repressor (ICER) is a transcriptional repressor, which, because of alternate promoter use, is generated from the 3' region of the cAMP response modulator (Crem) gene. Its expression and nuclear occurrence are elevated by high cAMP levels in naturally occurring regulatory T cells (nTregs). Using tw...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vaeth M,Gogishvili T,Bopp T,Klein M,Berberich-Siebelt F,Gattenloehner S,Avots A,Sparwasser T,Grebe N,Schmitt E,Hünig T,Serfling E,Bodor J

更新日期：2011-02-08 00:00:00

abstract：:The plasma iron turnover was measured in 19 normal subjects. A correlation was found between plasma iron concentration and plasma iron turnover. In addition to the turnover of 55Fe at normal plasma iron concentration (predominantly monoferric transferrin), a second turnover in which the labeled plasma was saturated wi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Skarberg K,Eng M,Huebers H,Marsaglia G,Finch C

更新日期：1978-03-01 00:00:00

abstract：:In this report we examine the effects of chronic anaerobic exposure and subsequent reoxygenation on protein synthesis patterns in Chinese hamster ovary cells. It is observed by two-dimensional gel electrophoresis (isoelectric focusing/NaDodSO4/PAGE) that the transition from an atmospheric environment to an anaerobic s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sciandra JJ,Subjeck JR,Hughes CS

更新日期：1984-08-01 00:00:00

abstract：:Of the four major types of Ca channel described in vertebrate cells (designated T, L, N, and P), N-type Ca channels are unique in that they are found specifically in neurons, have been correlated with control of neurotransmitter release, and are blocked by omega-conotoxin, a neuropeptide toxin isolated from the marine...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dubel SJ,Starr TV,Hell J,Ahlijanian MK,Enyeart JJ,Catterall WA,Snutch TP

更新日期：1992-06-01 00:00:00

abstract：:Spontaneous regression/complete resistance (SR/CR) mice resist very high doses of cancer cells that are lethal to WT mice even at low doses. In this study, we show that this resistance is mediated by rapid infiltration of leukocytes, mostly of innate immunity, in both primary and repeated challenges. Formation of rose...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hicks AM,Riedlinger G,Willingham MC,Alexander-Miller MA,Von Kap-Herr C,Pettenati MJ,Sanders AM,Weir HM,Du W,Kim J,Simpson AJ,Old LJ,Cui Z

更新日期：2006-05-16 00:00:00

abstract：:The minimal size of the recombination site required for efficient FLP recombinase-catalyzed recombination in vitro is no more than 28 base pairs, which includes parts of two 13-base-pair inverted repeats and all of an 8-base-pair spacer. The FLP recombinase cleaves the DNA at the boundaries of the spacer, becomes cova...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Senecoff JF,Bruckner RC,Cox MM

更新日期：1985-11-01 00:00:00

abstract：:The 5' flanking sequences and the intron of the mouse Hoxa-7 gene were searched for regulatory elements that can function in Drosophila. Only the intron is able to activate a lacZ fusion gene in various tissues of Drosophila embryos. This enhancer function requires a cluster of three homeodomain binding sites (HB1-ele...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haerry TE,Gehring WJ

更新日期：1996-11-26 00:00:00

abstract：:We studied the coupled binding and folding of alpha-synuclein, an intrinsically disordered protein linked with Parkinson's disease. Using single-molecule fluorescence resonance energy transfer and correlation methods, we directly probed protein membrane association, structural distributions, and dynamics. Results reve...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ferreon AC,Gambin Y,Lemke EA,Deniz AA

更新日期：2009-04-07 00:00:00

abstract：:A study of the near-infrared absorption spectra of three oxygen compounds of membrane-bound cytochrome oxidase (ferrocytochrome c:oxygen oxidoreductase; EC 1.9.3.1) shows that the formation of compound A (oxycytochrome oxidase) causes no significant infrared absorbance changes at -103 degrees. At -64 degrees, the form...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chance B,Leigh JS Jr

更新日期：1977-11-01 00:00:00

abstract：:Lysinuric protein intolerance (LPI) is an autosomal recessive phenotype consistent with impaired transport of cationic amino acids at the basolateral membrane of intestinal and renal epithelia. On the assumption that the basolateral membrane of epithelial cells and plasma membrane of parenchymal cells are functional a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith DW,Scriver CR,Tenenhouse HS,Simell O

更新日期：1987-11-01 00:00:00