Oxygen intermediates and mixed valence states of cytochrome oxidase: infrared absorption difference spectra of compounds A, B, and C of cytochrome oxidase and oxygen.

abstract：:Endosomal cargo travels through a dynamic vesicle network en route to degradation by lysosomes or recycling through the Golgi apparatus back to the cell surface. Rab5 is a key determinant of the early endosomes by organizing effector proteins in specific subdomains and mediating early endosome fusion. We find that ear...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Antignani A,Youle RJ

更新日期：2008-06-10 00:00:00

abstract：:We have used a semiselective Hahn spin-echo sequence of the form (1331)-tau-(2662)-tau-AQ, delivered by a surface coil to obtain high-resolution 1H NMR spectra from the brains of intact dead rats. This sequence gave suppression of the tissue water resonance by a factor of 80,000 when tau = 68 ms. Delivery of a frequen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hetherington HP,Avison MJ,Shulman RG

更新日期：1985-05-01 00:00:00

abstract：:The baculovirus Autographa californica nuclear polyhedrosis virus (AcMNPV, which is representative of the MNPV subtype in which the virions may contain many nucleocapsids within a single viral envelope) encodes a protein, v-ubi, that has 76% identity with the eukaryotic protein ubiquitin. Transcriptional mapping indic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guarino LA

更新日期：1990-01-01 00:00:00

abstract：:The conformation of the pressure-dissociated monomer of Arc repressor was characterized by 1H NMR spectroscopy. The NMR spectra of the monomer under pressure (up to 5.0 kbar; 1 bar = 100 kPa) are typical of a molten globule and they are considerably different from those of the native dimer and thermally denatured mono...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peng X,Jonas J,Silva JL

更新日期：1993-03-01 00:00:00

abstract：:In aqueous solution, aldehydes, and to a lesser extent ketones, hydrate to form geminal diols. We investigate the hydration of methylglyoxal (MG) in the gas phase, a process not previously considered to occur in water-restricted environments. In this study, we spectroscopically identified methylglyoxal diol (MGD) and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Axson JL,Takahashi K,De Haan DO,Vaida V

更新日期：2010-04-13 00:00:00

abstract：:The chloroplast coupling factor 1 consists of five nonidentical subunits, three of which (alpha, beta, and epsilon subunits) have been shown in several laboratories to be synthesized within chloroplasts. The site of synthesis of the remaining two (gamma and delta subunits) was investigated by analyzing products direct...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Watanabe A,Price CA

更新日期：1982-10-01 00:00:00

abstract：:Methods are described for the removal of the sporophytic pollen grain coating of Brassica oleracea and for the isolation of coat polypeptides. The coat contains a small number of proteins ranging from 6 to 45 kDa. Many of the larger proteins are glycosylated, while all carry high positive charges resulting in pI value...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Doughty J,Hedderson F,McCubbin A,Dickinson H

更新日期：1993-01-15 00:00:00

abstract：:"Functional connectivity" techniques are commonplace tools for studying brain organization. A critical element of these analyses is to distinguish variance due to neurobiological signals from variance due to nonneurobiological signals. Multiecho fMRI techniques are a promising means for making such distinctions based ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Power JD,Plitt M,Gotts SJ,Kundu P,Voon V,Bandettini PA,Martin A

更新日期：2018-02-27 00:00:00

abstract：:X-linked dystonia-parkinsonism (XDP) is a neurodegenerative disease associated with an antisense insertion of a SINE-VNTR-Alu (SVA)-type retrotransposon within an intron of TAF1 This unique insertion coincides with six additional noncoding sequence changes in TAF1, the gene that encodes TATA-binding protein-associated...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bragg DC,Mangkalaphiban K,Vaine CA,Kulkarni NJ,Shin D,Yadav R,Dhakal J,Ton ML,Cheng A,Russo CT,Ang M,Acuña P,Go C,Franceour TN,Multhaupt-Buell T,Ito N,Müller U,Hendriks WT,Breakefield XO,Sharma N,Ozelius LJ

更新日期：2017-12-19 00:00:00

abstract：:Sex is thought to facilitate accumulation of initially rare beneficial mutations by allowing simultaneous allele replacements at many loci. However, this advantage of sex depends on a restrictive assumption that the fitness of a genotype is determined by fitness potential, a single intermediate variable to which all l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kondrashov FA,Kondrashov AS

更新日期：2001-10-09 00:00:00

abstract：:The ability to grow the marine molluse Aplysia under laboratory conditions allows a detailed study of the formation of the nervous system and of the development of specific identified cells. I have found that the ganglia develop in a specific temporal order. Cerebral and pedal ganglia develop at hatching, the abdomina...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kriegstein AR

更新日期：1977-01-01 00:00:00

abstract：:Epstein-Barr virus-induced gene 3 (EBI3) is a widely expressed IL-12p40-related protein that associates as a heterodimer with either IL-12p35 or an IL-12p35 homologue, p28, to create a new cytokine (IL-27). To define the function of EBI3 in vivo, we generated knockout mice in which the ebi3 gene was targeted by homolo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nieuwenhuis EE,Neurath MF,Corazza N,Iijima H,Trgovcich J,Wirtz S,Glickman J,Bailey D,Yoshida M,Galle PR,Kronenberg M,Birkenbach M,Blumberg RS

更新日期：2002-12-24 00:00:00

abstract：:We have identified a murine gene, metaxin, that spans the 6-kb interval separating the glucocerebrosidase gene (GC) from the thrombospondin 3 gene on chromosome 3E3-F1. Metaxin and GC are transcribed convergently; their major polyadenylylation sites are only 431 bp apart. On the other hand, metaxin and the thrombospon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bornstein P,McKinney CE,LaMarca ME,Winfield S,Shingu T,Devarayalu S,Vos HL,Ginns EI

更新日期：1995-05-09 00:00:00

abstract：:Schizosaccharomyces pombe cells were transformed with an S. pombe expression vector containing a full-length cDNA of the Chlorella hexose transporter. The transformed cells accumulated 3-O-methylglucose up to 10-fold, whereas wild-type S. pombe and control transformants could only equilibrate this sugar analogue. In a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sauer N,Caspari T,Klebl F,Tanner W

更新日期：1990-10-01 00:00:00

abstract：:Escherichia coli K12 2320(lambda)-15B has a mutation that results in ochre suppressor activity.(1) This mutation concomitantly causes a decreased growth rate in rich medium, an increased sensitivity to streptomycin,(1) and the production of some altered 30S ribosomes which are differentially sensitive to RNase.(2) The...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gartner TK,Orias E,Lannan JE,Beeson J,Reid PJ

更新日期：1969-03-01 00:00:00

abstract：:Human skeletal muscle is composed of various muscle fiber types. We hypothesized that differences in metabolism between fiber types could be detected noninvasively with 31P nuclear magnetic resonance spectroscopy during maximal exercise. This assumes that during maximal exercise all fiber types are recruited and all v...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vandenborne K,McCully K,Kakihira H,Prammer M,Bolinger L,Detre JA,De Meirlier K,Walter G,Chance B,Leigh JS

更新日期：1991-07-01 00:00:00

abstract：:Thiol-dependent redox regulation allows the rapid adaptation of chloroplast function to unpredictable changes in light intensity. Traditionally, it has been considered that chloroplast redox regulation relies on photosynthetically reduced ferredoxin (Fd), thioredoxins (Trxs), and an Fd-dependent Trx reductase (FTR), t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pérez-Ruiz JM,Naranjo B,Ojeda V,Guinea M,Cejudo FJ

更新日期：2017-11-07 00:00:00

abstract：:A climate/vegetation model simulates episodic wetter and drier periods at the 21,000-y precession period in eastern North Africa, the Arabian Peninsula, and the Levant over the past 140,000 y. Large orbitally forced wet/dry extremes occur during interglacial time, ∼130 to 80 ka, and conditions between these two extrem...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kutzbach JE,Guan J,He F,Cohen AS,Orland IJ,Chen G

更新日期：2020-02-04 00:00:00

abstract：:Histone methylation has an important role in transcriptional regulation. However, unlike H3K4 and H3K9 methylation, the role of H4K20 monomethylation (H4K20me-1) in transcriptional regulation remains unclear. Here, we show that Wnt3a specifically stimulates H4K20 monomethylation at the T cell factor (TCF)-binding elem...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li Z,Nie F,Wang S,Li L

更新日期：2011-02-22 00:00:00

abstract：:Control of the establishment of cell polarity is an essential function in tissue morphogenesis and renewal that depends on spatial cues provided by the extracellular environment. The molecular role of cell-cell or cell-extracellular matrix (ECM) contacts on the establishment of cell polarity has been well characterize...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Théry M,Racine V,Piel M,Pépin A,Dimitrov A,Chen Y,Sibarita JB,Bornens M

更新日期：2006-12-26 00:00:00

abstract：:Characterization and functional annotation of the large number of proteins predicted from genome sequencing projects poses a major scientific challenge. Whereas several proteomics techniques have been developed to quantify the abundance of proteins, these methods provide little information regarding protein function. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Okerberg ES,Wu J,Zhang B,Samii B,Blackford K,Winn DT,Shreder KR,Burbaum JJ,Patricelli MP

更新日期：2005-04-05 00:00:00

abstract：:Mass spectrometry (MS)-based quantitative interaction proteomics has successfully elucidated specific protein-protein, DNA-protein, and small molecule-protein interactions. Here, we developed a gel-free, sensitive, and scalable technology that addresses the important area of RNA-protein interactions. Using aptamer-tag...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Butter F,Scheibe M,Mörl M,Mann M

更新日期：2009-06-30 00:00:00

abstract：:Understanding plant metabolism as an integrated system is essential for metabolic engineering aimed at the effective production of compounds useful to human life and the global environment. The "omics" approach integrates transcriptome and metabolome data into a single data set and can lead to the identification of un...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hirai MY,Sugiyama K,Sawada Y,Tohge T,Obayashi T,Suzuki A,Araki R,Sakurai N,Suzuki H,Aoki K,Goda H,Nishizawa OI,Shibata D,Saito K

更新日期：2007-04-10 00:00:00

abstract：:Decision-making in a natural environment depends on a hierarchy of interacting decision processes. A high-level strategy guides ongoing choices, and the outcomes of those choices determine whether or not the strategy should change. When the right decision strategy is uncertain, as in most natural settings, feedback be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Purcell BA,Kiani R

更新日期：2016-08-02 00:00:00

abstract：:Normal rat kidney cells were nonproductively infected either with CP27, a mutant of Moloney sarcoma virus that is temperature-sensitive for maintenance of transformation, or with the parental wild-type virus. The nonproducer cells were inoculated into the tails of athymic nude mice that were subsequently incubated at ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Klarlund JK,Forchhammer J

更新日期：1980-03-01 00:00:00

abstract：:To investigate how T cells recognize allogeneic class I proteins encoded by the major histocompatibility complex (MHC), we examined the human cytotoxic T lymphocytes (CTL) elicited in a mixed lymphocyte reaction against a lymphoblastoid B-cell line (JY) whose MHC-class I proteins are HLA-A2 and -B7. By panning the res...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Elliott TJ,Eisen HN

更新日期：1988-04-01 00:00:00

abstract：:An inductive interaction between the sevenless (sev) transmembrane tyrosine kinase receptor and the bride of sevenless (boss) transmembrane ligand is required for the development of the R7 photoreceptor neuron in the compound eye of Drosophila melanogaster. The boss protein is proposed to contain a large N-terminal ex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hart AC,Harrison SD,Van Vactor DL Jr,Rubin GM,Zipursky SL

更新日期：1993-06-01 00:00:00

abstract：:The auditory cortex is critical for perceiving a sound's location. However, there is no topographic representation of acoustic space, and individual auditory cortical neurons are often broadly tuned to stimulus location. It thus remains unclear how acoustic space is represented in the mammalian cerebral cortex and how...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miller LM,Recanzone GH

更新日期：2009-04-07 00:00:00

abstract：:Homology-directed repair (HDR) is a critical pathway for the repair of DNA double-strand breaks (DSBs) in mammalian cells. Efficient HDR is thought to be crucial for maintenance of genomic integrity during organismal development and tumor suppression. However, most mammalian HDR studies have focused on transformed and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kass EM,Helgadottir HR,Chen CC,Barbera M,Wang R,Westermark UK,Ludwig T,Moynahan ME,Jasin M

更新日期：2013-04-02 00:00:00

abstract：:Chromophore-assisted light inactivation (CALI) offers the only method capable of modulating specific protein activities in localized regions and at particular times. Here, we generalize CALI so that it can be applied to a wider range of tasks. Specifically, we show that CALI can work with a genetically inserted epitop...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Surrey T,Elowitz MB,Wolf PE,Yang F,Nédélec F,Shokat K,Leibler S

更新日期：1998-04-14 00:00:00