Abstract:
:Characterization and functional annotation of the large number of proteins predicted from genome sequencing projects poses a major scientific challenge. Whereas several proteomics techniques have been developed to quantify the abundance of proteins, these methods provide little information regarding protein function. Here, we present a gel-free platform that permits ultrasensitive, quantitative, and high-resolution analyses of protein activities in proteomes, including highly problematic samples such as undiluted plasma. We demonstrate the value of this platform for the discovery of both disease-related enzyme activities and specific inhibitors that target these proteins.
journal_name
Proc Natl Acad Sci U S Aauthors
Okerberg ES,Wu J,Zhang B,Samii B,Blackford K,Winn DT,Shreder KR,Burbaum JJ,Patricelli MPdoi
10.1073/pnas.0501205102keywords:
subject
Has Abstractpub_date
2005-04-05 00:00:00pages
4996-5001issue
14eissn
0027-8424issn
1091-6490pii
0501205102journal_volume
102pub_type
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