Interplay of alpha-synuclein binding and conformational switching probed by single-molecule fluorescence.

abstract：:The lck gene encodes a membrane-associated protein tyrosine kinase (p56lck) that is believed to participate in lymphocyte-specific signal transduction pathways. To investigate the function of this molecule, transgenic mice were generated carrying the wild-type lck gene or a mutated lck gene encoding a constitutively a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Abraham KM,Levin SD,Marth JD,Forbush KA,Perlmutter RM

更新日期：1991-05-01 00:00:00

abstract：:pRK212.2, a derivative of the broad host range plasmid RK2, contains two EcoRI cleavage fragments, A and B, neither of which can replicate by itself in Escherichia coli. Fragment A (41.7 kilobases), but not fragment B (14.4 kilobases), can be cloned by insertion into the unrelated plasmids mini-F and ColE1. Fragment B...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Figurski DH,Helinski DR

更新日期：1979-04-01 00:00:00

abstract：:A PCR strategy is described for global amplification of DNA from a single eukaryotic cell that enables the comprehensive analysis of the whole genome. By comparative genomic hybridization, not only gross DNA copy number variations, such as monosomic X and trisomic 21 in single male cells and cells from Down's syndrome...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Klein CA,Schmidt-Kittler O,Schardt JA,Pantel K,Speicher MR,Riethmüller G

更新日期：1999-04-13 00:00:00

abstract：:Let X = Omega/Gamma be a compact quotient of an irreducible bounded symmetric domain Omega of rank >/=2 by a discrete group omega of automorphisms without fixed points. It is well known that the Kähler-Einstein metric g on X carries seminegative curvature (in the sense of Griffiths). I show that any Hermitian metric h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mok N

更新日期：1986-04-01 00:00:00

abstract：:We have developed an all-electronic digital microfluidic device for microscale chemical synthesis in organic solvents, operated by electrowetting-on-dielectric (EWOD). As an example of the principles, we demonstrate the multistep synthesis of [(18)F]FDG, the most common radiotracer for positron emission tomography (PE...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keng PY,Chen S,Ding H,Sadeghi S,Shah GJ,Dooraghi A,Phelps ME,Satyamurthy N,Chatziioannou AF,Kim CJ,van Dam RM

更新日期：2012-01-17 00:00:00

abstract：:Opportunistic infections are often polymicrobial. Two of the most important bacterial opportunistic pathogens of humans, Pseudomonas aeruginosa and Staphylococcus aureus, frequently are coisolated from infections of catheters, endotracheal tubes, skin, eyes, and the respiratory tract, including the airways of people w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoffman LR,Déziel E,D'Argenio DA,Lépine F,Emerson J,McNamara S,Gibson RL,Ramsey BW,Miller SI

更新日期：2006-12-26 00:00:00

abstract：:The chromatin core particle DNA conformation deduced in broad outline by Finch et al. [Finch, J. T., Lutter, L. C., Rhodes, D., Brown, R. S., Rushton, B., Levitt, M. & Klug, A. (1977) Nature 269, 29-36] can be described in detail using other available experimental results. Histone binding sites compatible with the pat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carter CW Jr

更新日期：1978-08-01 00:00:00

abstract：:Pancreatic ductal adenocarcinoma (PDAC) remains a highly lethal disease in critical need of new therapeutic strategies. Here, we report that the stress-inducible 78-kDa glucose-regulated protein (GRP78/HSPA5), a key regulator of endoplasmic reticulum homeostasis and PI3K/AKT signaling, is overexpressed in the acini an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shen J,Ha DP,Zhu G,Rangel DF,Kobielak A,Gill PS,Groshen S,Dubeau L,Lee AS

更新日期：2017-05-16 00:00:00

abstract：:Many bacteria use acyl homoserine lactone signals to monitor cell density in a type of gene regulation termed quorum sensing and response. Synthesis of these signals is directed by homologs of the luxi gene of Vibrio fischeri. This communication resolves two critical issues concerning the synthesis of the V. fischeri ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schaefer AL,Val DL,Hanzelka BL,Cronan JE Jr,Greenberg EP

更新日期：1996-09-03 00:00:00

abstract：:Thrombin induces platelet aggregation and formation of a fibrin clot in platelet-rich plasma; leupeptin, a protease inhibitor, partially inhibits platelet aggregation, but it does not inhibit fibrin clot formation. Indomethacin does not inhibit either thrombin-induced platelet aggregation or fibrin clot formation. How...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ruggiero M,Lapetina EG

更新日期：1986-05-01 00:00:00

abstract：:Protein kinases play key roles in the induction by human interferon alpha (IFN-alpha) of specific gene expression and biological activity in various human cell lines. We now report that IFN-alpha increased the 7-kb transcript for the epsilon isotype of protein kinase C (PKC-epsilon) and the cellular content of PKC-eps...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang C,Constantinescu SN,MacEwan DJ,Strulovici B,Dekker LV,Parker PJ,Pfeffer LM

更新日期：1993-08-01 00:00:00

abstract：:Although genetic analysis has demonstrated that members of the winged helix, or forkhead, family of transcription factors play pivotal roles in the regulation of cellular differentiation and proliferation, both during development and in the adult, little is known of the mechanisms underlying their regulation. Here we ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Biggs WH 3rd,Meisenhelder J,Hunter T,Cavenee WK,Arden KC

更新日期：1999-06-22 00:00:00

abstract：:It is becoming common practice to partition glass-forming liquids into two classes based on the dependence of the shear viscosity η on temperature T. In an Arrhenius plot, ln η vs 1/T, a strong liquid shows linear behavior whereas a fragile liquid exhibits an upward curvature [super-Arrhenius (SA) behavior], a situati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mallamace F,Branca C,Corsaro C,Leone N,Spooren J,Chen SH,Stanley HE

更新日期：2010-12-28 00:00:00

abstract：:Stroke patients with small central nervous system infarcts often demonstrate an acute dysexecutive syndrome characterized by difficulty with attention, concentration, and processing speed, independent of lesion size or location. We use magnetoencephalography (MEG) to show that disruption of network dynamics may be res...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marsh EB,Brodbeck C,Llinas RH,Mallick D,Kulasingham JP,Simon JZ,Llinás RR

更新日期：2020-12-29 00:00:00

abstract：:Transmembrane (TM) domains of integral membrane proteins are generally thought to be helical. However, a Gly-Pro sequence within the TM domain of the insulin receptor is predicted to act as a helix breaker. CD analyses of model TM peptides in a lipid-like environment show that substitution of Gly and Pro by Ala enhanc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goncalves E,Yamada K,Thatte HS,Backer JM,Golan DE,Kahn CR,Shoelson SE

更新日期：1993-06-15 00:00:00

abstract：:Somatostatin (somatotropin release-inhibiting factor; SRIF) is a tetradecapeptide present in brain, pancreas, gastrointestinal tract, and thyroid that inhibits the secretion or action of several hormones in these tissues. We observed that the toad urinary bladder contains concentrations of endogenous somatostatin (8.0...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Forrest JN Jr,Reichlin S,Goodman DB

更新日期：1980-08-01 00:00:00

abstract：:A recent major conceptual advance has been the recognition of the importance of immune system-neuronal interactions in the modulation of brain function, one example of which is spinal pain processing in neuropathic states. Here, we report that in peripheral nerve-injured rats, the lysosomal cysteine protease cathepsin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clark AK,Yip PK,Grist J,Gentry C,Staniland AA,Marchand F,Dehvari M,Wotherspoon G,Winter J,Ullah J,Bevan S,Malcangio M

更新日期：2007-06-19 00:00:00

abstract：:Dopamine-beta-hydroxylase (EC 1.14.2.1), an enzyme localized in sympathetic synaptic vesicles, was released together with norepinephrine during in vitro stimulation of the hypogastric nerve that innervates the vas deferens of the guinea pig. Stimulation of the nerve in the presence of high concentrations of calcium (7...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Johnson DG,Thoa NB,Weinshilboum R,Axelrod J,Kopin IJ

更新日期：1971-09-01 00:00:00

abstract：:We report dynamic glycoprotein recognition of gels prepared by biomolecular imprinting using lectin and antibody molecules as ligands for tumor-specific marker glycoproteins. The glycoprotein-imprinted gels prepared with minute amounts of cross-linkers could dynamically recognize tumor-specific marker glycoproteins by...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miyata T,Jige M,Nakaminami T,Uragami T

更新日期：2006-01-31 00:00:00

abstract：:The major histocompatibility complex class-I (MHC-I) peptide-loading complex (PLC) is a cornerstone of the human adaptive immune system, being responsible for processing antigens that allow killer T cells to distinguish between healthy and compromised cells. Based on a recent low-resolution cryo-electron microscopy (c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fisette O,Schröder GF,Schäfer LV

更新日期：2020-08-25 00:00:00

abstract：:Activation of the androgen receptor (AR) may play a role in androgen-independent progression of prostate cancer. Multiple mechanisms of AR activation, including stimulation by tyrosine kinases, have been postulated. We and others have recently shown involvement of activated Cdc42-associated tyrosine kinase Ack1 in adv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mahajan NP,Liu Y,Majumder S,Warren MR,Parker CE,Mohler JL,Earp HS,Whang YE

更新日期：2007-05-15 00:00:00

abstract：:To modify the lac permease of Escherichia coli for fluorescence spectroscopy, six tryptophan residues at positions 10, 33, 78, 151, 171, and 223 were first replaced individually with phenylalanine by using oligonucleotide-directed site-specific mutagenesis. None of the tryptophan residues is critical for activity, as ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Menezes ME,Roepe PD,Kaback HR

更新日期：1990-03-01 00:00:00

abstract：:Alzheimer's disease is characterized by two primary pathological features: amyloid plaques and neurofibrillary tangles. The interconnection between amyloid and tau aggregates is of intense interest, but mouse models have yet to reveal a direct interrelationship. We now show that NO may be a key factor that connects am...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Colton CA,Vitek MP,Wink DA,Xu Q,Cantillana V,Previti ML,Van Nostrand WE,Weinberg JB,Dawson H

更新日期：2006-08-22 00:00:00

abstract：:During the entry process, the human papillomavirus (HPV) capsid is trafficked to the trans-Golgi network (TGN), whereupon it enters the nucleus during mitosis. We previously demonstrated that the minor capsid protein L2 assumes a transmembranous conformation in the TGN. Here we provide evidence that the incoming viral...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： DiGiuseppe S,Luszczek W,Keiffer TR,Bienkowska-Haba M,Guion LG,Sapp MJ

更新日期：2016-05-31 00:00:00

abstract：:Charcot-Marie-Tooth disease type 4C (CMT4C) is an early-onset, autosomal recessive form of demyelinating neuropathy. The clinical manifestations include progressive scoliosis, delayed age of walking, muscular atrophy, distal weakness, and reduced nerve conduction velocity. The gene mutated in CMT4C disease, SH3TC2/KIA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arnaud E,Zenker J,de Preux Charles AS,Stendel C,Roos A,Médard JJ,Tricaud N,Kleine H,Luscher B,Weis J,Suter U,Senderek J,Chrast R

更新日期：2009-10-13 00:00:00

abstract：:Most neurotransmission is mediated by action potentials, whereas sensory neurons propagate electrical signals passively and release neurotransmitter in a graded manner. Here, we demonstrate that Caenorhabditis elegans neuromuscular junctions release neurotransmitter in a graded fashion. When motor neurons were depolar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu Q,Hollopeter G,Jorgensen EM

更新日期：2009-06-30 00:00:00

abstract：:Integrated hepatitis B virus (HBV) DNA is almost invariably found in hepatocellular carcinomas (HCC) which develop in HBV carriers. Integrated HBV DNAs from two single-integration HCCs (C3 and C4) have been cloned, and the cellular integration sites have been analyzed. Integrated HBV DNA of C3 is present in chromosome...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hino O,Shows TB,Rogler CE

更新日期：1986-11-01 00:00:00

abstract：:In order to understand the functions of simian virus 40 genes, permissive cells (TC7) were infected with mutants temperature sensitive in the complementation groups A, B, C, BC, and D at permissive and nonpermissive temperatures. Cells were examined for the localization of viral polypeptide antigens by immunofluoresce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kasamatsu H,Nehorayan A

更新日期：1979-06-01 00:00:00

abstract：:Ubiquitylation of histone H2B at lysine 120 (H2B-Ub) plays a critical role in transcriptional elongation, chromatin conformation, as well as the regulation of specific histone H3 methylations. Herein, we report a strategy for the site-specific chemical attachment of ubiquitin to preassembled nucleosomes. This allowed ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holt MT,David Y,Pollock S,Tang Z,Jeon J,Kim J,Roeder RG,Muir TW

更新日期：2015-08-18 00:00:00

abstract：:Peripheral blood leukocytes, obtained from volunteers after vaccination or natural illness with influenza, were assayed for cytotoxicity against influenza virus-infected cells. Approximately 7 days after vaccination or the onset of respiratory illness, peak cytotoxicity was demonstrated in a chromium-release assay. Se...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Greenberg SB,Six HR,Drake S,Couch RB

更新日期：1979-09-01 00:00:00