当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Optimizing transmembrane domain helicity accelerates insulin receptor internalization and lateral mobility.

Optimizing transmembrane domain helicity accelerates insulin receptor internalization and lateral mobility.

Abstract：

:Transmembrane (TM) domains of integral membrane proteins are generally thought to be helical. However, a Gly-Pro sequence within the TM domain of the insulin receptor is predicted to act as a helix breaker. CD analyses of model TM peptides in a lipid-like environment show that substitution of Gly and Pro by Ala enhances helicity. On this basis, Gly933 and Pro934 within the TM domain of the intact human insulin receptor were mutated to Ala (G-->A, P-->A, GP-->AA) to assess effects of altered helicity on receptor functions. Mutated and wild-type receptors, expressed stably in cultured CHO cells at equivalent levels, were properly assembled, biosynthetically processed, and exhibited similar affinities for insulin. Receptor autophosphorylation and substrate kinase activity in intact cells and soluble receptor preparations were indistinguishable. In contrast, insulin-stimulated receptor internalization was accelerated 2-fold for the GP-->AA mutant, compared to a wild-type control or the G-->A and P-->A mutants. Insulin degradation, which occurs during receptor endocytosis and recycling, was similarly elevated in cells transfected with GP-->AA mutant receptors. Fluorescence photobleaching recovery measurements showed that the lateral mobility of GP-->AA mutant receptors was also increased 2- to 3-fold. These results suggest that lateral mobility directly influences rates of insulin-mediated receptor endocytosis and that rates of endocytosis and lateral mobility are retarded by a kinked TM domain in the wild-type receptor. Invariance of Gly-Pro within insulin receptor TM domain sequences suggests a physiologic advantage for submaximal rates of receptor internalization.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Goncalves E,Yamada K,Thatte HS,Backer JM,Golan DE,Kahn CR,Shoelson SE

doi

10.1073/pnas.90.12.5762

subject

Has Abstract

pub_date

1993-06-15 00:00:00

pages

5762-6

issue

12

eissn

0027-8424

issn

1091-6490

journal_volume

90

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Amplified dihydrofolate reductase genes in unstably methotrexate-resistant cells are associated with double minute chromosomes.

    abstract：:Selection of mammalian cells in progressively increasing concentrations of methotrexate results in selective amplification of DNA sequences coding for dihydrofolate reductase (tetrahydrofolate dehydrogenase, 5,6,7,8-tetrahydrofolate:NADP+ oxidoreductase, EC 1.5.1.3). In some cell variants the amplified genes are stabl...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.76.11.5669

    authors： Kaufman RJ,Brown PC,Schimke RT

    更新日期：1979-11-01 00:00:00

  • Crystal structure of the Helicobacter pylori vacuolating toxin p55 domain.

    abstract：:Helicobacter pylori VacA, a pore-forming toxin secreted by an autotransporter pathway, causes multiple alterations in human cells, contributes to the pathogenesis of peptic ulcer disease and gastric cancer, and is a candidate antigen for inclusion in an H. pylori vaccine. Here, we present a 2.4-A crystal structure of ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0707447104

    authors： Gangwer KA,Mushrush DJ,Stauff DL,Spiller B,McClain MS,Cover TL,Lacy DB

    更新日期：2007-10-09 00:00:00

  • Sodium azide mutagenesis: preferential generation of A.T-->G.C transitions in the barley Ant18 gene.

    abstract：:The molecular basis for the absence of anthocyanins and proanthocyanidins in four independent sodium azide-induced ant18 mutants of barley was examined by sequencing the gene encoding dihydroflavonol 4-reductase in these mutants. Sodium azide generated 21 base substitutions, which corresponds to 0.17% of the 12,704 nu...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.17.8043

    authors： Olsen O,Wang X,von Wettstein D

    更新日期：1993-09-01 00:00:00

  • Different G protein-coupled receptor kinases govern G protein and beta-arrestin-mediated signaling of V2 vasopressin receptor.

    abstract：:Signaling through beta-arrestins is a recently appreciated mechanism used by seven-transmembrane receptors. Because G protein-coupled receptor kinase (GRK) phosphorylation of such receptors is generally a prerequisite for beta-arrestin binding, we studied the roles of different GRKs in promoting beta-arrestin-mediated...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0409534102

    authors： Ren XR,Reiter E,Ahn S,Kim J,Chen W,Lefkowitz RJ

    更新日期：2005-02-01 00:00:00

  • Regulation of transferrin-mediated iron uptake by HFE, the protein defective in hereditary hemochromatosis.

    abstract：:The protein defective in hereditary hemochromatosis, called HFE, is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). Its association with beta2M was previously shown to be necessary for its stability, normal intracellular processing, and cell surface expression in transfected COS ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.042701499

    authors： Waheed A,Grubb JH,Zhou XY,Tomatsu S,Fleming RE,Costaldi ME,Britton RS,Bacon BR,Sly WS

    更新日期：2002-03-05 00:00:00

  • Two pharmacologically distinct sodium- and chloride-coupled high-affinity gamma-aminobutyric acid transporters are present in plasma membrane vesicles and reconstituted preparations from rat brain.

    abstract：:Electrogenic sodium- and chloride-dependent gamma-aminobutyric acid (GABA) transport in crude synaptosomal membrane vesicles is partly inhibited by saturating levels of either of the substrate analogues cis-3-aminocyclohexanecarboxylic acid (ACHC) or beta-alanine. However, both of them together potently and fully inhi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.7.2550

    authors： Kanner BI,Bendahan A

    更新日期：1990-04-01 00:00:00

  • Invariant representations of visual patterns in a temporal population code.

    abstract：:Mammalian visual systems are characterized by their ability to recognize stimuli invariant to various transformations. Here, we investigate the hypothesis that this ability is achieved by the temporal encoding of visual stimuli. By using a model of a cortical network, we show that this encoding is invariant to several...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0136977100

    authors： Wyss R,Konig P,Verschure PF

    更新日期：2003-01-07 00:00:00

  • Dielectric constant of atomic fluids with variable polarizability.

    abstract：:The Clausius-Mossotti function for the dielectric constant is expanded in terms of single atom and pair polarizabilities, leading to contributions that depend on both the trace and the anisotropy of the pair-polarizability tensor. The short-range contribution of the anisotropic part to the pair polarizabilities has pr...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.77.6.3098

    authors： Alder BJ,Beers JC,Strauss HL,Weis JJ

    更新日期：1980-06-01 00:00:00

  • The c-ring stoichiometry of ATP synthase is adapted to cell physiological requirements of alkaliphilic Bacillus pseudofirmus OF4.

    abstract：:The c-rings of ATP synthases consist of individual c-subunits, all of which harbor a conserved motif of repetitive glycine residues (GxGxGxG) important for tight transmembrane α-helix packing. The c-ring stoichiometry determines the number of ions transferred during enzyme operation and has a direct impact on the ion-...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1303333110

    authors： Preiss L,Klyszejko AL,Hicks DB,Liu J,Fackelmayer OJ,Yildiz Ö,Krulwich TA,Meier T

    更新日期：2013-05-07 00:00:00

  • An RNA polymerase II transcription factor has an associated DNA-dependent ATPase (dATPase) activity strongly stimulated by the TATA region of promoters.

    abstract：:A transcription factor required for synthesis of accurately initiated run-off transcripts by RNA polymerase II has been purified and shown to have an associated DNA-dependent ATPase (dATPase) activity that is strongly stimulated by the TATA region of promoters. This transcription factor, designated delta, was purified...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.19.7356

    authors： Conaway RC,Conaway JW

    更新日期：1989-10-01 00:00:00

  • Global variability in seawater Mg:Ca and Sr:Ca ratios in the modern ocean.

    abstract：:Seawater Mg:Ca and Sr:Ca ratios are biogeochemical parameters reflecting the Earth-ocean-atmosphere dynamic exchange of elements. The ratios' dependence on the environment and organisms' biology facilitates their application in marine sciences. Here, we present a measured single-laboratory dataset, combined with previ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1918943117

    authors： Lebrato M,Garbe-Schönberg D,Müller MN,Blanco-Ameijeiras S,Feely RA,Lorenzoni L,Molinero JC,Bremer K,Jones DOB,Iglesias-Rodriguez D,Greeley D,Lamare MD,Paulmier A,Graco M,Cartes J,Barcelos E Ramos J,de Lara A,Sanchez-Lea

    更新日期：2020-09-08 00:00:00

  • Microbial carbohydrate depolymerization by antigen-presenting cells: deamination prior to presentation by the MHCII pathway.

    abstract：:After uptake by the endosome of an antigen-presenting cell (APC), exogenous proteins are known to be degraded into peptides by protease digestion. Here, we report the mechanism by which pure carbohydrates can be depolymerized within APC endosomes/lysosomes by nitric oxide (NO)-derived reactive nitrogen species (RNSs) ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0800974105

    authors： Duan J,Avci FY,Kasper DL

    更新日期：2008-04-01 00:00:00

  • Pre-B cell receptor expression is necessary for thymic stromal lymphopoietin responsiveness in the bone marrow but not in the liver environment.

    abstract：:IL-7 and thymic stromal lymphopoietin (TSLP) are two major cytokines controlling murine B cell development. IL-7 has been studied extensively, but only recently has it become possible to unravel the role of TSLP in detail. We studied the biological activities of TSLP in B cell development at distinct ages in the mouse...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0402919101

    authors： Vosshenrich CA,Cumano A,Müller W,Di Santo JP,Vieira P

    更新日期：2004-07-27 00:00:00

  • Wolbachia uses a host microRNA to regulate transcripts of a methyltransferase, contributing to dengue virus inhibition in Aedes aegypti.

    abstract：:The endosymbiont Wolbachia is common among insects and known for the reproductive manipulations it exerts on hosts as well as inhibition of virus replication in their hosts. Recently, we showed that Wolbachia uses host microRNAs to manipulate host gene expression for its efficient maintenance in the dengue mosquito ve...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1303603110

    authors： Zhang G,Hussain M,O'Neill SL,Asgari S

    更新日期：2013-06-18 00:00:00

  • Inhibition of retinoic acid-induced activation of 3' human HOXB genes by antisense oligonucleotides affects sequential activation of genes located upstream in the four HOX clusters.

    abstract：:Most homeobox genes belonging to the Hox family are sequentially activated in embryonal carcinoma cells upon treatment with retinoic acid. Genes located at the 3' end of each one of the four Hox clusters are activated first, whereas upstream Hox genes are activated progressively later. This activation has been extensi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.12.5335

    authors： Faiella A,Zappavigna V,Mavilio F,Boncinelli E

    更新日期：1994-06-07 00:00:00

  • Antibodies and IL-3 support helminth-induced basophil expansion.

    abstract：:Basophils are powerful mediators of Th2 immunity and are present in increased numbers during allergic inflammation and helminth infection. Despite their ability to potentiate Th2 immunity the mechanisms regulating basophil development remain largely unknown. We have found a unique role for isotype-switched antibodies ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1117584109

    authors： Herbst T,Esser J,Prati M,Kulagin M,Stettler R,Zaiss MM,Hewitson JP,Merky P,Verbeek JS,Bourquin C,Camberis M,Prout M,Maizels RM,Le Gros G,Harris NL

    更新日期：2012-09-11 00:00:00

  • Impact and cost-effectiveness of new tuberculosis vaccines in low- and middle-income countries.

    abstract：:To help reach the target of tuberculosis (TB) disease elimination by 2050, vaccine development needs to occur now. We estimated the impact and cost-effectiveness of potential TB vaccines in low- and middle-income countries using an age-structured transmission model. New vaccines were assumed to be available in 2024, t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1404386111

    authors： Knight GM,Griffiths UK,Sumner T,Laurence YV,Gheorghe A,Vassall A,Glaziou P,White RG

    更新日期：2014-10-28 00:00:00

  • Anomalous and selective DNA mutations of the Old World monkey alpha-globin genes.

    abstract：:It has been a widely accepted hypothesis that the molecular clock slows down during evolution of higher primates. By molecular cloning and nucleotide sequence comparison of a rhesus macaque alpha-globin gene to its homologs in man, orangutan, olive baboon, and other mammals, we demonstrate a burst of evolution of the ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.4.1312

    authors： Shaw JP,Marks J,Shen CC,Shen CK

    更新日期：1989-02-01 00:00:00

  • Association of IL28B gene variations with mathematical modeling of viral kinetics in chronic hepatitis C patients with IFN plus ribavirin therapy.

    abstract：:Asian patients with chronic hepatitis C (CHC) are known to have better virological responses to pegylated (Peg) IFN-based therapy than Western patients. Although IL28B gene polymorphisms may contribute to this difference, whether favorable hepatitis C virus (HCV) kinetics during treatment plays a role remains unclear....

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 临床试验,杂志文章

    doi：10.1073/pnas.1100349108

    authors： Hsu CS,Hsu SJ,Chen HC,Tseng TC,Liu CH,Niu WF,Jeng J,Liu CJ,Lai MY,Chen PJ,Kao JH,Chen DS

    更新日期：2011-03-01 00:00:00

  • REM sleep's unique associations with corticosterone regulation, apoptotic pathways, and behavior in chronic stress in mice.

    abstract：:One of sleep's putative functions is mediation of adaptation to waking experiences. Chronic stress is a common waking experience; however, which specific aspect of sleep is most responsive, and how sleep changes relate to behavioral disturbances and molecular correlates remain unknown. We quantified sleep, physical, e...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1816456116

    authors： Nollet M,Hicks H,McCarthy AP,Wu H,Möller-Levet CS,Laing EE,Malki K,Lawless N,Wafford KA,Dijk DJ,Winsky-Sommerer R

    更新日期：2019-02-12 00:00:00

  • Peroxisome proliferator-activated receptor delta promotes very low-density lipoprotein-derived fatty acid catabolism in the macrophage.

    abstract：:Significant attention has focused on the role of low-density lipoprotein (LDL) in the pathogenesis of atherosclerosis. However, recent advances have identified triglyceride-rich lipoproteins [e.g., very LDL (VLDL)] as independent risk predictors for this disease. We have previously demonstrated peroxisome proliferator...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0510815103

    authors： Lee CH,Kang K,Mehl IR,Nofsinger R,Alaynick WA,Chong LW,Rosenfeld JM,Evans RM

    更新日期：2006-02-14 00:00:00

  • Astrocyte elevated gene-1 (AEG-1) functions as an oncogene and regulates angiogenesis.

    abstract：:Astrocyte-elevated gene-1 (AEG-1) expression is increased in multiple cancers and plays a central role in Ha-ras-mediated oncogenesis through the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway. Additionally, overexpression of AEG-1 protects primary and transformed human and rat cells from serum starvation-...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0910936106

    authors： Emdad L,Lee SG,Su ZZ,Jeon HY,Boukerche H,Sarkar D,Fisher PB

    更新日期：2009-12-15 00:00:00

  • Human CUL1 forms an evolutionarily conserved ubiquitin ligase complex (SCF) with SKP1 and an F-box protein.

    abstract：:The SCF ubiquitin ligase complex of budding yeast triggers DNA replication by catalyzing ubiquitination of the S phase cyclin-dependent kinase inhibitor SIC1. SCF is composed of three proteins-ySKP1, CDC53 (Cullin), and the F-box protein CDC4-that are conserved from yeast to humans. As part of an effort to identify co...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.13.7451

    authors： Lyapina SA,Correll CC,Kipreos ET,Deshaies RJ

    更新日期：1998-06-23 00:00:00

  • Kaposi's sarcoma-associated herpesvirus interacts with EphrinA2 receptor to amplify signaling essential for productive infection.

    abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV), etiologically associated with Kaposi's sarcoma, uses integrins (α3β1, αVβ3, and αVβ5) and associated signaling to enter human dermal microvascular endothelial cells (HMVEC-d), an in vivo target of infection. KSHV infection activated c-Cbl, which induced the selective tra...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1119592109

    authors： Chakraborty S,Veettil MV,Bottero V,Chandran B

    更新日期：2012-05-08 00:00:00

  • Nonspecific binding to structured RNA and preferential unwinding of an exposed helix by the CYT-19 protein, a DEAD-box RNA chaperone.

    abstract：:We explore the interactions of CYT-19, a DExD/H-box protein that functions in folding of group I RNAs, with a well characterized misfolded species of the Tetrahymena ribozyme. Consistent with its function, CYT-19 accelerates refolding of the misfolded RNA to its native state. Unexpectedly, CYT-19 performs another reac...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0603127103

    authors： Tijerina P,Bhaskaran H,Russell R

    更新日期：2006-11-07 00:00:00

  • Myosin-1E interacts with FAK proline-rich region 1 to induce fibronectin-type matrix.

    abstract：:Focal adhesion kinase (FAK) is a nonreceptor tyrosine kinase involved in development and human disease, including cancer. It is currently thought that the four-point one, ezrin, radixin, moesin (FERM)-kinase domain linker, which contains autophosphorylation site tyrosine (Y) 397, is not required for in vivo FAK functi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1614894114

    authors： Heim JB,Squirewell EJ,Neu A,Zocher G,Sominidi-Damodaran S,Wyles SP,Nikolova E,Behrendt N,Saunte DM,Lock-Andersen J,Gaonkar KS,Yan H,Sarkaria JN,Krendel M,van Deursen J,Sprangers R,Stehle T,Böttcher RT,Lee JH,Ordog T

    更新日期：2017-04-11 00:00:00

  • Human medial gastrocnemius force-velocity behavior shifts with locomotion speed and gait.

    abstract：:Humans walk and run over a wide range of speeds with remarkable efficiency. For steady locomotion, moving at different speeds requires the muscle-tendon units of the leg to modulate the amount of mechanical power the limb absorbs and outputs in each step. How individual muscles adapt their behavior to modulate limb po...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 临床试验,杂志文章

    doi：10.1073/pnas.1107972109

    authors： Farris DJ,Sawicki GS

    更新日期：2012-01-17 00:00:00

  • Why we need to look beyond the glass transition temperature to characterize the dynamics of thin supported polymer films.

    abstract：:There is significant variation in the reported magnitude and even the sign of [Formula: see text] shifts in thin polymer films with nominally the same chemistry, film thickness, and supporting substrate. The implicit assumption is that methods used to estimate [Formula: see text] in bulk materials are relevant for inf...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1722024115

    authors： Zhang W,Douglas JF,Starr FW

    更新日期：2018-05-29 00:00:00

  • Oscillatory singular integrals and harmonic analysis on nilpotent groups.

    abstract：:Several related classes of operators on nilpotent Lie groups are considered. These operators involve the following features: (i) oscillatory factors that are exponentials of imaginary polynomials, (ii) convolutions with singular kernels supported on lower-dimensional submanifolds, (iii) validity in the general context...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.83.1.1

    authors： Ricci F,Stein EM

    更新日期：1986-01-01 00:00:00

  • Relationships between the responses of triglyceride-rich lipoproteins in blood plasma containing apolipoproteins B-48 and B-100 to a fat-containing meal in normolipidemic humans.

    abstract：:The concentration of triglyceride-rich lipoproteins containing apolipoprotein (apo) B-48 (chylomicrons) and apo B-100 (very low density lipoproteins) was measured in blood plasma of healthy young men after an ordinary meal containing one-third of daily energy and fat. Plasma obtained in the postabsorptive state and at...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.5.2069

    authors： Schneeman BO,Kotite L,Todd KM,Havel RJ

    更新日期：1993-03-01 00:00:00