Different G protein-coupled receptor kinases govern G protein and beta-arrestin-mediated signaling of V2 vasopressin receptor.

abstract：:For study of the basis of an X-linked form of gout in man, several clonal lines deficient in hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) were selected from the human lymphoblast line WI-L2 by spontaneous and mutagen-induced resistance to 10 muM 8-azaguanine. Three groups could be defined: (1) clones wi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lever JE,Nuki G,Seegmiller JE

更新日期：1974-07-01 00:00:00

abstract：:Female mosquitoes feed sequentially on carbohydrates (nectar) and proteins (blood) during each gonadotrophic cycle to become reproductively competent and effective disease vectors. Accordingly, metabolism is synchronized to support this reproductive cyclicity. However, regulatory pathways linking metabolism to reprodu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ling L,Raikhel AS

更新日期：2021-02-09 00:00:00

abstract：:Ovine pulmonary surfactant is bactericidal for Pasteurella haemolytica when surfactant and bacteria mixtures are incubated with normal ovine serum. To isolate this component, surfactant (1 mg/ml) was centrifuged at 100,000 x gav, and the supernatant was fractionated by HPLC. Fractions were eluted with acetonitrile (10...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brogden KA,De Lucca AJ,Bland J,Elliott S

更新日期：1996-01-09 00:00:00

abstract：:The DNA end-joining protein Ku70 is one of several proteins that inhibit apoptosis by sequestering the proapoptotic factor Bax from the mitochondria. However, the molecular mechanism underlying Ku70-dependent inhibition of Bax is not fully understood. Here, we show that the absence of Ku70 results in the accumulation ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Amsel AD,Rathaus M,Kronman N,Cohen HY

更新日期：2008-04-01 00:00:00

abstract：:We have isolated several lines of rat embryo cells transformed by simian virus 40. All these lines are fully transformed with regard to saturation density and serum sensitivity, but they differ greatly in their anchorage dependence, as assayed by efficiency of plating in methyl cellulose suspension. This set of lines ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pollack R,Risser R,Conlon S,Rifkin D

更新日期：1974-12-01 00:00:00

abstract：:These experiments investigate the capacity of thalamic and cortical structures in a sensory system to process information of a modality normally associated with another system. Retinal ganglion cells in newborn Syrian hamsters were made to project permanently to the main thalamic somatosensory (ventrobasal) nucleus. W...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Métin C,Frost DO

更新日期：1989-01-01 00:00:00

abstract：:We describe the use of the matrix eigenvalue decomposition (EVD) and pseudoinverse projection and a tensor higher-order EVD (HOEVD) in reconstructing the pathways that compose a cellular system from genome-scale nondirectional networks of correlations among the genes of the system. The EVD formulates a genes x genes n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alter O,Golub GH

更新日期：2005-12-06 00:00:00

abstract：:In this article, we address the problem of modeling generic features of structurally but not textually related DNA motifs, that is, motifs whose consensus sequences are entirely different but nevertheless share "metasequence features" reflecting similarities in the DNA-binding domains of their associated protein recog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xing EP,Karp RM

更新日期：2004-07-20 00:00:00

abstract：:Protein tyrosine phosphorylation in purified synaptic vesicles from rat forebrain has been studied in the presence of Mn2+ and orthovanadate. High levels of endogenous protein tyrosine phosphorylation were observed. Four major phosphoproteins, with apparent molecular masses of 105, 94, 38, and 30 kDa, were shown to co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pang DT,Wang JK,Valtorta F,Benfenati F,Greengard P

更新日期：1988-02-01 00:00:00

abstract：:An antibody (anti-p21ser) was raised against a ras p21-related synthetic peptide and was able to recognize specifically the substitution of serine for glycine at amino acid 12 of p21. This substitution causes oncogenic activation of p21. Anti-p21ser was found to immunoprecipitate v-Ki-ras p21 and to strongly inhibit i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clark R,Wong G,Arnheim N,Nitecki D,McCormick F

更新日期：1985-08-01 00:00:00

abstract：:The envelope spike of HIV is one of the most highly N-glycosylated structures found in nature. However, despite extensive research revealing essential functional roles in infection and immune evasion, the chemical structures of the glycans on the native viral envelope glycoprotein gp120--as opposed to recombinantly ge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Doores KJ,Bonomelli C,Harvey DJ,Vasiljevic S,Dwek RA,Burton DR,Crispin M,Scanlan CN

更新日期：2010-08-03 00:00:00

abstract：:The nitrogen isotopic composition (15N/14N) of forested ecosystems varies systematically worldwide. In tropical forests, which are elevated in 15N relative to temperate biomes, a decrease in ecosystem 15N/14N with increasing rainfall has been reported. This trend is seen in a set of well characterized Hawaiian rainfor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Houlton BZ,Sigman DM,Hedin LO

更新日期：2006-06-06 00:00:00

abstract：:Through use of a synthetic oligonucleotide probe, human insulin-like growth factor I (IGF-I) cDNA clones were isolated from a liver library. Two types of cDNAs were defined by restriction enzyme analysis and DNA sequencing. Both encode IGF-I precursors of either 195 or 153 amino acids. The two predicted protein precur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rotwein P

更新日期：1986-01-01 00:00:00

abstract：:Phylogenies describe the origins and history of species. However, they can also help to predict species' fates and so can be useful tools for managing the future of biodiversity. This article starts by sketching how phylogenetic, geographic, and trait information can be combined to elucidate present mammalian diversit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Davies TJ,Fritz SA,Grenyer R,Orme CD,Bielby J,Bininda-Emonds OR,Cardillo M,Jones KE,Gittleman JL,Mace GM,Purvis A

更新日期：2008-08-12 00:00:00

abstract：:The adenoviral E1A oncogene sensitizes mammalian cells to tumor necrosis factor-alpha (TNF-alpha), in part by repressing the nuclear factor-kappa B (NF-kappa B)-dependent defense against this cytokine. Other E1A activities involve binding to either p300/cyclic AMP response element-binding protein (CBP) or retinoblasto...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cook JL,Walker TA,Worthen GS,Radke JR

更新日期：2002-07-23 00:00:00

abstract：:The genes encoding the histone acetyl-transferases (HATs) CREB binding protein (CREBBP) and EP300 are recurrently mutated in the activated B cell-like and germinal center (GC) B cell-like subtypes of diffuse large B cell lymphoma (DLBCL). Here, we introduced a patient mutation into a human DLBCL cell line using CRISPR...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hashwah H,Schmid CA,Kasser S,Bertram K,Stelling A,Manz MG,Müller A

更新日期：2017-09-05 00:00:00

abstract：:The symbiotic bacterium Wolbachia pipientis has been considered unique in its ability to cause multiple reproductive anomalies in its arthropod hosts. Here we report that an undescribed bacterium is vertically transmitted and associated with thelytokous parthenogenetic reproduction in Encarsia, a genus of parasitoid w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zchori-Fein E,Gottlieb Y,Kelly SE,Brown JK,Wilson JM,Karr TL,Hunter MS

更新日期：2001-10-23 00:00:00

abstract：:The earliest measurable parameter that shows density-dependent inhibition is the uptake of [(3)H]deoxyglucose by Balb 3T3 cells. The rate decreases even during the exponential phase of growth and reaches a minimum, about 8- to 10-times lower than maximum, as the culture approaches the saturation density. Cells transfo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bose SK,Zlotnick BJ

更新日期：1973-08-01 00:00:00

abstract：:VIP21/caveolin is localized to both caveolae and apical transport vesicles and presumably cycles between the cell surface and the Golgi complex. We have studied the lipid interactions of this protein by reconstituting Escherichia coli-expressed VIP21/caveolin into liposomes. Surprisingly, the protein reconstituted onl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murata M,Peränen J,Schreiner R,Wieland F,Kurzchalia TV,Simons K

更新日期：1995-10-24 00:00:00

abstract：:In bacteria, the first two steps of gene expression-transcription and translation-are spatially and temporally coupled. Uncoupling may lead to the arrest of transcription through RNA polymerase backtracking, which interferes with replication forks, leading to DNA double-stranded breaks and genomic instability. How tra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stevenson-Jones F,Woodgate J,Castro-Roa D,Zenkin N

更新日期：2020-04-14 00:00:00

abstract：:Antigen receptor gene assembly is regulated by transcriptional promoters and enhancers, which control the accessibility of gene segments to a lymphocyte-specific V(D)J recombinase. However, it remained unclear whether accessibility depends on the process of transcription itself or chromatin modifications that accompan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sikes ML,Meade A,Tripathi R,Krangel MS,Oltz EM

更新日期：2002-09-17 00:00:00

abstract：:Src tyrosine kinase has long been implicated in colon cancer but much remains to be learned about its substrates. The nuclear receptor hepatocyte nuclear factor 4α (HNF4α) has just recently been implicated in colon cancer but its role is poorly defined. Here we show that c-Src phosphorylates human HNF4α on three tyros...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chellappa K,Jankova L,Schnabl JM,Pan S,Brelivet Y,Fung CL,Chan C,Dent OF,Clarke SJ,Robertson GR,Sladek FM

更新日期：2012-02-14 00:00:00

abstract：:The 72-kDa spleen tyrosine kinase (Syk) and Src-family kinase p53/56Lyn (Lyn) contribute to signaling via the B-cell antigen receptor complex. Here we show that Syk and Lyn from human B lymphocytes can interact directly. Syk and Lyn coimmunoprecipitated from mature and activated B-cell lines, and gel-purified Syk and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sidorenko SP,Law CL,Chandran KA,Clark EA

更新日期：1995-01-17 00:00:00

abstract：:Neutrinos represent a new "window" to the Universe, spanning a large range of energy. We discuss the science of neutrino astrophysics and focus on two energy regimes. At "lower" energies ( approximately 1 MeV), studies of neutrinos born inside the sun, or produced in interactions of cosmic rays with the atmosphere, ha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Besson D,Cowen D,Selen M,Wiebusch C

更新日期：1999-12-07 00:00:00

abstract：:The E6 oncoprotein of human papillomaviruses (HPVs) that are associated with cervical cancer utilizes the cellular ubiquitin-protein ligase E6-AP to target the tumor suppressor p53 for degradation. In normal cells (i.e., in the absence of E6), p53 is also a target of the ubiquitin-proteasome pathway. Under these condi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hengstermann A,Linares LK,Ciechanover A,Whitaker NJ,Scheffner M

更新日期：2001-01-30 00:00:00

abstract：:Functional MRI based on blood oxygenation level-dependent (BOLD) contrast is well established as a neuroimaging technique for detecting neural activity in the cortex of the human brain. While detection and characterization of BOLD signals, as well as their electrophysiological and hemodynamic/metabolic origins, have b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ding Z,Huang Y,Bailey SK,Gao Y,Cutting LE,Rogers BP,Newton AT,Gore JC

更新日期：2018-01-16 00:00:00

abstract：:Bacteriophage lambda integrates into the chromosome of its Escherichia coli host by means of a site-specific recombination between a locus on the phage chromosome (phage att site) and a locus on the bacterial chromosome (bacterial att site). The nucleotide sequence of four lambda att sites altered in site-specific rec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoess RH,Landy A

更新日期：1978-11-01 00:00:00

abstract：:Two methods for increasing the length of DNA sequence data that can be read off a polyacrylamide gel are described. We have developed a rapid way to pour a buffer concentration gradient gel that, by altering the vertical band separation on an autoradiograph, allows more sequence to be obtained from a gel. We also show...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Biggin MD,Gibson TJ,Hong GF

更新日期：1983-07-01 00:00:00

abstract：:Mitogen-stimulated scheduled DNA synthesis and DNA excision repair in human lymphocytes, as well as DNA polymerase a activity in a cell-free system, were inhibited by an electrophilic metabolite of benzo[a]pyrene. This metabolite, (+/-)-anti-(7r,8t)-dihydroxy-(9,10t)-epoxy-7,8,9,10-tetrahyd robenzo[a]pyrene (BPDE), co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Busbee DL,Joe CO,Norman JO,Rankin PW

更新日期：1984-09-01 00:00:00

abstract：:We have examined the process by which small groups of pigmented germinal cells transplanted orthotopically from stage 30-38 donor embryos into stage 28-38 albino hosts contribute new postmitotic cells to the pigmented retinal epithelium of the growing larval eye in Xenopus. In the great majority of chimeric eyes, the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hunt RK,Cohen JS,Mason BJ

更新日期：1987-05-01 00:00:00