Incoming human papillomavirus type 16 genome resides in a vesicular compartment throughout mitosis.

abstract：:A sensitive assay using biotinylated ubiquitin revealed extensive ubiquitination of the large subunit of RNA polymerase II during incubations of transcription reactions in vitro. Phosphorylation of the repetitive carboxyl-terminal domain of the large subunit was a signal for ubiquitination. Specific inhibitors of cycl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitsui A,Sharp PA

更新日期：1999-05-25 00:00:00

abstract：:Heat-shock protein of 90 kDa (Hsp90) is an essential molecular chaperone that adopts different 3D structures associated with distinct nucleotide states: a wide-open, V-shaped dimer in the apo state and a twisted, N-terminally closed dimer with ATP. Although the N domain is known to mediate ATP binding, how Hsp90 sense...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sung N,Lee J,Kim JH,Chang C,Joachimiak A,Lee S,Tsai FT

更新日期：2016-03-15 00:00:00

abstract：:Animals are associated with a microbiome that can affect their reproductive success. It is, therefore, important to understand how a host and its microbiome coevolve. According to the hologenome concept, hosts and their microbiome form an integrated evolutionary entity, a holobiont, on which selection can potentially ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： van Vliet S,Doebeli M

更新日期：2019-10-08 00:00:00

abstract：:The effect of pH on the membrane-fusion activity of Sendai virus was examined (pH 5.0-9.5) by using, as assays of activity, hemolysis of chicken erythrocytes and the fusion of baby hamster kidney (BHK-21) cells. Exposure of virus to basic pH increased fusion activity; the optimum pH was found to be approximately equal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hsu MC,Scheid A,Choppin PW

更新日期：1982-10-01 00:00:00

abstract：:Temperature lability of ADP-glucose pyrophosphorylase (AGP; glucose-1-phosphate adenylyltransferase; ADP: alpha-D-glucose-1-phosphate adenylyltransferase, EC 2.7.7.27), a key starch biosynthetic enzyme, may play a significant role in the heat-induced loss in maize seed weight and yield. Here we report the isolation an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Greene TW,Hannah LC

更新日期：1998-10-27 00:00:00

abstract：:Components in cerebrospinal fluid that are antigenically related to myelin basic protein have been identified by a technique described recently [Barbarese, E., Braun, P. E. & Carson, J. H. (1977) Proc. Natl. Acad. Sci. USA 74, 3360-3364] involving separating the cerebrospinal fluid proteins by sodium dodecyl sulfate/p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carson JH,Barbarese E,Braun PE,McPherson TA

更新日期：1978-04-01 00:00:00

abstract：:In the mammalian testis, the blood-testis barrier (BTB), unlike the blood-brain and blood-retina barriers, is composed of coexisting tight junctions (TJs) and adherens junctions (AJs). Yet these junctions must open (or disassemble) to accommodate the migration of preleptotene and leptotene spermatocytes across the BTB...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yan HH,Cheng CY

更新日期：2005-08-16 00:00:00

abstract：:Hepatocyte growth factor (HGF) is an activating ligand of the Met receptor tyrosine kinase, whose activity is essential for normal tissue development and organ regeneration but abnormal activation of Met has been implicated in growth, invasion, and metastasis of many types of solid tumors. HGF has two natural splice v...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tolbert WD,Daugherty-Holtrop J,Gherardi E,Vande Woude G,Xu HE

更新日期：2010-07-27 00:00:00

abstract：:The molecular mechanisms by which transcription is selectively activated and precisely controlled by signal transducer and activator of transcription (Stat) factors represent a central issue in cytokine-mediated cellular responses. Stat6 mediates responses to IL-4 and antagonizes Stat1 activated by IFN-gamma. We have ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goenka S,Boothby M

更新日期：2006-03-14 00:00:00

abstract：:Look-through mutagenesis (LTM) is a multidimensional mutagenesis method that simultaneously assesses and optimizes combinatorial mutations of selected amino acids. The process focuses on a precise distribution within one or more complementarity determining region (CDR) domains and explores the synergistic contribution...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rajpal A,Beyaz N,Haber L,Cappuccilli G,Yee H,Bhatt RR,Takeuchi T,Lerner RA,Crea R

更新日期：2005-06-14 00:00:00

abstract：:Expression of the human beta amyloid peptide (A beta) in transgenic Caenorhabditis elegans animals can lead to the formation of intracellular immunoreactive deposits as well as the formation of intracellular amyloid. We have used this model to identify proteins that interact with intracellular A beta in vivo. Mass spe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fonte V,Kapulkin WJ,Taft A,Fluet A,Friedman D,Link CD

更新日期：2002-07-09 00:00:00

abstract：:Rapid electrical conduction in the His-Purkinje system tightly controls spatiotemporal activation of the ventricles. Although recent work has shed much light on the regulation of early specification and morphogenesis of the His-Purkinje system, less is known about how transcriptional regulation establishes impulse con...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang SS,Kim KH,Rosen A,Smyth JW,Sakuma R,Delgado-Olguín P,Davis M,Chi NC,Puviindran V,Gaborit N,Sukonnik T,Wylie JN,Brand-Arzamendi K,Farman GP,Kim J,Rose RA,Marsden PA,Zhu Y,Zhou YQ,Miquerol L,Henkelman RM,Sta

更新日期：2011-08-16 00:00:00

abstract：:A polypeptide transforming growth factor (TGF) that induces anchorage-dependent rat kidney fibroblasts to grow in soft agar has been isolated from human placenta and purified to homogeneity. This polypeptide is classified as a type beta TGF because it does not compete with epidermal growth factor (EGF) for membrane re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frolik CA,Dart LL,Meyers CA,Smith DM,Sporn MB

更新日期：1983-06-01 00:00:00

abstract：:In mitosis, chromosome, cytoskeleton, and organelle dynamics must be coordinated for successful cell division. Here, we present evidence for a role for Arf1, a small GTPase associated with the Golgi apparatus, in the orchestration of mitotic Golgi breakdown, chromosome segregation, and cytokinesis. We show that early ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Altan-Bonnet N,Phair RD,Polishchuk RS,Weigert R,Lippincott-Schwartz J

更新日期：2003-11-11 00:00:00

abstract：:Chronic pancreatitis (CP) is a progressive inflammatory disease of the pancreas, leading to its fibrotic destruction. There are currently no drugs that can stop or slow the progression of the disease. The etiology of the disease is multifactorial, whereas recurrent attacks of acute pancreatitis are thought to precede ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hao W,Komar HM,Hart PA,Conwell DL,Lesinski GB,Friedman A

更新日期：2017-05-09 00:00:00

abstract：:The temporal distribution of the major extinctions over the past 250 million years has been investigated statistically using various forms of time series analysis. The analyzed record is based on variation in extinction intensity for fossil families of marine vertebrates, invertebrates, and protozoans and contains 12 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raup DM,Sepkoski JJ Jr

更新日期：1984-02-01 00:00:00

abstract：:The tightly coupled nature of the reaction sequence catalyzed by monoterpene cyclases has precluded direct observation of the topologically required isomerization step leading from geranyl pyrophosphate to the presumptive, enzyme-bound, tertiary allylic intermediate linalyl pyrophosphate, which ultimately cyclizes to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wheeler CJ,Croteau RB

更新日期：1987-07-01 00:00:00

abstract：:A water-soluble component of membranes of cells from a Burkitt's lymphoma cultured cell line was used to develop an antiserum that detects an antigen(s) on acute leukemia cells. This antiserum did not react with nonlymphoid cultured cell lines except for human embryonic kidney cells infected with the Rauscher murine l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mann DL,Halterman R,Leventhal BG

更新日期：1973-02-01 00:00:00

abstract：:Recent advances in fluorescence microscopy provided tools for the investigation and the analysis of the viral replication steps in the cellular context. In the HIV field, the current visualization systems successfully achieve the fluorescent labeling of the viral envelope and proteins, but not the genome. Here, we dev...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Di Primio C,Quercioli V,Allouch A,Gijsbers R,Christ F,Debyser Z,Arosio D,Cereseto A

更新日期：2013-04-02 00:00:00

abstract：:Sonic hedgehog (Shh) plays an integral role in both the anterior-posterior (A-P) patterning and expansion of developing vertebrate limbs through a feedback loop involving Fgfs, Bmps, and Gremlin. In bat limbs A-P patterning and the size of the digital field are unique. The posterior digits of the forelimb are elongate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hockman D,Cretekos CJ,Mason MK,Behringer RR,Jacobs DS,Illing N

更新日期：2008-11-04 00:00:00

abstract：:Epigenetic markers, such as histone acetylation and DNA methylation, determine chromatin organization. In eukaryotic cells, metabolites from organelles or the cytosol affect epigenetic modifications. However, the relationships between metabolites and epigenetic modifications are not well understood in plants. We found...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang L,Wang C,Liu X,Cheng J,Li S,Zhu JK,Gong Z

更新日期：2019-05-21 00:00:00

abstract：:Ca(2+) is essential for physiological depolarization-evoked synchronous neurotransmitter release. But, whether Ca(2+) influx or another factor controls release initiation is still under debate. The time course of ACh release is controlled by a presynaptic inhibitory G protein-coupled autoreceptor (GPCR), whose agonist...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kupchik YM,Rashkovan G,Ohana L,Keren-Raifman T,Dascal N,Parnas H,Parnas I

更新日期：2008-03-18 00:00:00

abstract：:Protein Hal is a human gamma4 heavy-chain disease protein whose molecular weight is reduced from 55,000 to 25,000 in 6 M guanidine due to the lack of disulfide bonds between heavy chains. Studies of aminoacid sequence indicate that it contains a gap of about 240 residues, starting 10 residues from the N-terminal end a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frangione B,Lee L,Haber E,Bloch KJ

更新日期：1973-04-01 00:00:00

abstract：:Antischizophrenic neuroleptic drugs of the diphenylbutylpiperidine class, which includes pimozide, fluspirilene, penfluridol, and clopimozide, inhibit [3H]nitrendipine binding with IC50 values of 13-30 nM. This inhibition involves receptors for the verapamil/prenylamine class of calcium channel antagonists. These diph...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gould RJ,Murphy KM,Reynolds IJ,Snyder SH

更新日期：1983-08-01 00:00:00

abstract：:Antibodies to electrolectin, a lectin endogenous to embryonic skeletal muscle, have been used to study the distribution of electrolectin during myogenesis in L6 cells and rat primary muscle cultures. Antibody binding is highest to mononucleated cells and is low to myotubes in both systems. Binding is much lower to fib...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Podleski TR,Greenberg I

更新日期：1980-02-01 00:00:00

abstract：:When the lactose (lac) permease of Escherichia coli is expressed from the lac promoter at relatively low rates, deletion of amino acid residues 2-8 (delta 7) or 2-9 (delta 8) from the hydrophilic N terminus has a relatively minor effect on the ability of the permease to catalyze active lactose transport. Activity is e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bibi E,Stearns SM,Kaback HR

更新日期：1992-04-15 00:00:00

abstract：:The maturation of 5S RNA in Escherichia coli is poorly understood. Although it is known that large precursors of 5S RNA accumulate in mutant cells lacking the endoribonuclease-RNase E, almost nothing is known about how the mature 5' and 3' termini of these molecules are generated. We have examined 5S RNA maturation in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li Z,Deutscher MP

更新日期：1995-07-18 00:00:00

abstract：:Escherichia coli infections, a leading cause of septic shock, remain a major threat to human health because of the fatal action to endotoxin (LPS). Therapeutic attempts to neutralize endotoxin currently focus on inhibiting the interaction of the toxic component lipid A with myeloid differentiating factor 2, which form...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gomery K,Müller-Loennies S,Brooks CL,Brade L,Kosma P,Di Padova F,Brade H,Evans SV

更新日期：2012-12-18 00:00:00

abstract：:A transient transfection assay using Drosophila S2 tissue culture cells and WT and mutant Drosophila vnd/NK-2 homeobox cDNAs was used to localize repression and activation domains of vnd/NK-2 homeodomain protein. A repression domain was identified near the N terminus of vnd/NK-2 homeodomain protein (amino acid residue...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stepchenko A,Nirenberg M

更新日期：2004-09-07 00:00:00

abstract：:In the human, cone photoreceptors (L, M, and S) and the melanopsin-containing, intrinsically photosensitive retinal ganglion cells (ipRGCs) are active at daytime light intensities. Signals from cones are combined both additively and in opposition to create the perception of overall light and color. Similar mechanisms ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spitschan M,Jain S,Brainard DH,Aguirre GK

更新日期：2014-10-28 00:00:00