Direct demonstration of the isomerization component of the monoterpene cyclase reaction using a cyclopropylcarbinyl pyrophosphate substrate analog.

abstract：:beta1,4-Galactosyltransferase (beta4GalT-I) participates in both glycoconjugate biosynthesis (ubiquitous activity) and lactose biosynthesis (mammary gland-specific activity). In somatic tissues, transcription of the mammalian beta4GalT-I gene results in a 4.1-kb mRNA and a 3.9-kb mRNA as a consequence of initiation at...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Charron M,Shaper JH,Shaper NL

更新日期：1998-12-08 00:00:00

abstract：:An essential element in the web-trap architecture, the capture silk spun by ecribellate orb spiders consists of glue droplets sitting astride a silk filament. Mechanically this thread presents a mixed solid-liquid behavior unknown to date. Under extension, capture silk behaves as a particularly stretchy solid, owing t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Elettro H,Neukirch S,Vollrath F,Antkowiak A

更新日期：2016-05-31 00:00:00

abstract：:Patchy particles with shape complementarity can serve as building blocks for assembling colloidal superstructures. Alternatively, encoding information on patches using DNA can direct assembly into a variety of crystalline or other preprogrammed structures. Here, we present a tool where DNA is used both to engineer sha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Diaz A JA,Oh JS,Yi GR,Pine DJ

更新日期：2020-05-19 00:00:00

abstract：:The LSD1-CoREST histone demethylase complex is required to repress neuronal genes in nonneuronal tissues. Here we show that sumoylation of Braf35, one of the subunits of the complex, is required to maintain full repression of neuron-specific genes and for occupancy of the LSD1-CoREST complex at its gene targets. Inter...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ceballos-Chávez M,Rivero S,García-Gutiérrez P,Rodríguez-Paredes M,García-Domínguez M,Bhattacharya S,Reyes JC

更新日期：2012-05-22 00:00:00

abstract：:Although Africa is the origin of modern humans, the pattern and distribution of genetic variation and correlations with cultural and linguistic diversity in Africa have been understudied. Recent advances in genomic technology, however, have led to genomewide studies of African samples. In this article, we discuss gene...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scheinfeldt LB,Soi S,Tishkoff SA

更新日期：2010-05-11 00:00:00

abstract：:Intracerebral hemorrhagic stroke and vascular dementia are age- and hypertension-associated manifestations of human cerebral small vessel disease (SVD). Cerebral microvessels are formed by endothelial cells (ECs), which are connected through tight junctions, adherens junctions, and stabilizing basement membrane struct...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weinl C,Castaneda Vega S,Riehle H,Stritt C,Calaminus C,Wolburg H,Mauel S,Breithaupt A,Gruber AD,Wasylyk B,Olson EN,Adams RH,Pichler BJ,Nordheim A

更新日期：2015-08-11 00:00:00

abstract：:Traumatic brain injury (TBI) causes neuronal apoptosis, inflammation, and reactive astrogliosis, which contribute to secondary tissue loss, impaired regeneration, and associated functional disabilities. Here, we show that up-regulation of cell cycle components is associated with caspase-mediated neuronal apoptosis and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Di Giovanni S,Movsesyan V,Ahmed F,Cernak I,Schinelli S,Stoica B,Faden AI

更新日期：2005-06-07 00:00:00

abstract：:The tetrodotoxin binding component of the voltage-sensitive sodium channel from Electrophorus electricus electroplax was purified by using a monoclonal antibody. An impure preparation of tetrodotoxin binding component was mixed with the pure monoclonal antibody, and the immune complex so formed was isolated by affinit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakayama H,Withy RM,Raftery MA

更新日期：1982-12-01 00:00:00

abstract：:Recent advances in understanding the role of neurotrophins on activity-dependent plasticity have provided insight into how behavior can affect specific aspects of neuronal biology. We present evidence that voluntary exercise can prime adult dorsal root ganglion neurons for increased axonal regeneration through a neuro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Molteni R,Zheng JQ,Ying Z,Gómez-Pinilla F,Twiss JL

更新日期：2004-06-01 00:00:00

abstract：:Transcriptional activation by steroid hormones is often associated with the appearance of a DNase I hypersensitive site resulting from a local alteration of the nucleoprotein structure of the promoter. For the mouse mammary tumor virus long terminal repeat, a viral promoter under glucocorticoid control, a model has be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carr KD,Richard-Foy H

更新日期：1990-12-01 00:00:00

abstract：:Noncompetitive inhibitors of the nicotinic acetylcholine (ACh) receptors suppress cation flux directly by binding in and blocking the open channel or indirectly by stabilizing closed states of the receptor. The lidocaine derivative QX-314 and the acridine derivative quinacrine act directly as open channel blockers, bu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu Y,Shi L,Karlin A

更新日期：2003-04-01 00:00:00

abstract：:Jasmonoyl-isoleucine (JA-Ile), the active form of the plant hormone jasmonate (JA), is sensed by the F-box protein CORONATINE INSENSITIVE 1 (COI1), a component of a functional Skp-Cullin-F-box E3 ubiquitin ligase complex. Sensing of JA-Ile by COI1 rapidly triggers genome-wide transcriptional changes that are largely r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： An C,Li L,Zhai Q,You Y,Deng L,Wu F,Chen R,Jiang H,Wang H,Chen Q,Li C

更新日期：2017-10-17 00:00:00

abstract：:Weakly oncogenic adenovirus 2 (Ad2)-transformed LSH hamster cells are sensitive to lysis by spontaneously cytolytic lymphoid cells and activated macrophages, whereas highly oncogenic simian virus 40 (SV40)-transformed LSH cells are relatively resistant to these nonspecific effector cells. Somatic cell hybrids formed b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cook JL,Hauser J,Patch CT,Lewis AM Jr,Levine AS

更新日期：1983-10-01 00:00:00

abstract：:Identifying potentially unique features of the human cerebral cortex is a first step to understanding how evolution has shaped the brain in our species. By analyzing MR images obtained from 177 humans and 73 chimpanzees, we observed a human-specific asymmetry in the superior temporal sulcus at the heart of the communi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leroy F,Cai Q,Bogart SL,Dubois J,Coulon O,Monzalvo K,Fischer C,Glasel H,Van der Haegen L,Bénézit A,Lin CP,Kennedy DN,Ihara AS,Hertz-Pannier L,Moutard ML,Poupon C,Brysbaert M,Roberts N,Hopkins WD,Mangin JF,Dehaene-

更新日期：2015-01-27 00:00:00

abstract：:To improve the efficiency of liposome-mediated DNA transfer as a tool for gene therapy, we have developed a fusigenic liposome vector based on principles of viral cell fusion. The fusion proteins of hemagglutinating virus of Japan (HVJ; also Sendai virus) are complexed with liposomes that encapsulate oligodeoxynucleot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dzau VJ,Mann MJ,Morishita R,Kaneda Y

更新日期：1996-10-15 00:00:00

abstract：:Following carcinogen exposure in vitro, normal rat tracheal epithelial cells are transformed in a multistage process in which the cultured cells become immortal and, ultimately, neoplastic. Five cell lines derived from tumors produced by neoplastically transformed rat tracheal epithelial cells were examined for the ex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walker C,Nettesheim P,Barrett JC,Gilmer TM

更新日期：1987-04-01 00:00:00

abstract：:Plasmids containing the replication origin of the Escherichia coli chromosome (oriC plasmids) are unstable in certain recA strains of E. coli. However, they can be maintained more stably in other recA strains. This stable maintenance has allowed us to study the incompatibility properties of oriC plasmids. We have foun...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamaguchi K,Yamaguchi M,Tomizawa J

更新日期：1982-09-01 00:00:00

abstract：:Several domains of the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein have been identified that are involved in HIV-1-mediated membrane fusion. One domain that is involved in membrane fusion is the hydrophobic amino terminus of the HIV-1 transmembrane glycoprotein gp41. Here we show that a polar sub...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Freed EO,Delwart EL,Buchschacher GL Jr,Panganiban AT

更新日期：1992-01-01 00:00:00

abstract：:Aphids maintain mutualistic symbioses involving consortia of coinherited organisms. All possess a primary endosymbiont, Buchnera, which compensates for dietary deficiencies; many also contain secondary symbionts, such as Hamiltonella defensa, which confers defense against natural enemies. Genome sequences of uncultiva...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moran NA,Degnan PH,Santos SR,Dunbar HE,Ochman H

更新日期：2005-11-22 00:00:00

abstract：:Bacterial capsules are surface layers made of long-chain polysaccharides. They are anchored to the outer membrane of many Gram-negative bacteria, including pathogens such as Escherichia coli, Neisseria meningitidis, Haemophilus influenzae, and Pasteurella multocida. Capsules protect pathogens from host defenses includ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Willis LM,Stupak J,Richards MR,Lowary TL,Li J,Whitfield C

更新日期：2013-05-07 00:00:00

abstract：:Rat hepatoma cells infected with mouse mammary tumor virus contain multiple forms of unintegrated viral DNA when grown in the presence of glucocorticoids. Using the DNA transfer procedure of Southern, we have prepared restriction endonuclease fragment maps of these forms of viral DNA. The maps indicate that: (i) the m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shank PR,Cohen JC,Varmus HE,Yamamoto KR,Ringold GM

更新日期：1978-05-01 00:00:00

abstract：:Juvenile hormone III (JH) plays a key role in regulating the reproduction of female mosquitoes. Microarray time-course analysis revealed dynamic changes in gene expression during posteclosion (PE) development in the fat body of female Aedes aegypti. Hierarchical clustering identified three major gene clusters: 1,843 e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zou Z,Saha TT,Roy S,Shin SW,Backman TW,Girke T,White KP,Raikhel AS

更新日期：2013-06-11 00:00:00

abstract：:The sided effects of H+ on Ca2(+)-stimulated K+ transport (the Gardos channel) were studied in human red blood cells. Cells were loaded with Ca2+ during energy depletion with the internal pH adjusted to desired levels prior to treatment with the anion-exchange inhibitor 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heinz A,Hoffman JF

更新日期：1990-03-01 00:00:00

abstract：:We have developed a culture system, simulating in vivo conditions of human immunodeficiency virus type 1 (HIV-1) infection, to evaluate the long-term efficacy of antisense oligonucleotide treatment. Five oligonucleotide phosphorothioates (28-mers), complementary to different regions of HIV-1 RNA, blocked replication o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lisziewicz J,Sun D,Klotman M,Agrawal S,Zamecnik P,Gallo R

更新日期：1992-12-01 00:00:00

abstract：:The phosphorylation and dephosphorylation of the dihydropyridine-sensitive Ca2+ channel was studied in transverse-tubule membranes isolated from rabbit skeletal muscle. Exposure of these membranes to either the cAMP-dependent protein kinase or a Ca2+/calmodulin-dependent protein kinase resulted in a rapid phosphorylat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hosey MM,Borsotto M,Lazdunski M

更新日期：1986-06-01 00:00:00

abstract：:Src homology 2 (SH2) domains are phosphotyrosine-binding modules found within various signal-transducing proteins. We have determined by 125I competition assay and surface plasmon resonance that the SH2 domains of Src and Lck bind to a variety of phosphopeptides with similar affinity and specificity. Both bound with h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Payne G,Shoelson SE,Gish GD,Pawson T,Walsh CT

更新日期：1993-06-01 00:00:00

abstract：:Infections caused by drug-resistant bacteria are a major problem worldwide. Carbapenem-resistant Klebsiella pneumoniae, most notably isolates classified as multilocus sequence type (ST) 258, have emerged as an important cause of hospital deaths. ST258 isolates are predominantly multidrug resistant, and therefore infec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Deleo FR,Chen L,Porcella SF,Martens CA,Kobayashi SD,Porter AR,Chavda KD,Jacobs MR,Mathema B,Olsen RJ,Bonomo RA,Musser JM,Kreiswirth BN

更新日期：2014-04-01 00:00:00

abstract：:Programmed cell death (apoptosis) is a normal physiological process, which could in principle be manipulated to play an important role in cancer therapy. The key importance of p53 expression in the apoptotic response to DNA-damaging agents has been stressed because mutant or deleted p53 is so common in most kinds of c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu C,Meikrantz W,Schlegel R,Sager R

更新日期：1995-08-15 00:00:00

abstract：:Characteristic patterns of gene expression measured by DNA microarrays have been used to classify tumors into clinically relevant subgroups. In this study, we have refined the previously defined subtypes of breast tumors that could be distinguished by their distinct patterns of gene expression. A total of 115 malignan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sorlie T,Tibshirani R,Parker J,Hastie T,Marron JS,Nobel A,Deng S,Johnsen H,Pesich R,Geisler S,Demeter J,Perou CM,Lønning PE,Brown PO,Børresen-Dale AL,Botstein D

更新日期：2003-07-08 00:00:00

abstract：:A fragment of the cloned gene for the human myeloma ND epsilon chain, coding for the second, third, and fourth domains of the immunoglobulin, has been coupled to the tryptophan control region of an expression plasmid and subcloned in Escherichia coli. Induction of gene expression results in the synthesis of the expect...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kenten J,Helm B,Ishizaka T,Cattini P,Gould H

更新日期：1984-05-01 00:00:00