当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Induction of glucose-regulated proteins during anaerobic exposure and of heat-shock proteins after reoxygenation.

Induction of glucose-regulated proteins during anaerobic exposure and of heat-shock proteins after reoxygenation.

Abstract：

:In this report we examine the effects of chronic anaerobic exposure and subsequent reoxygenation on protein synthesis patterns in Chinese hamster ovary cells. It is observed by two-dimensional gel electrophoresis (isoelectric focusing/NaDodSO4/PAGE) that the transition from an atmospheric environment to an anaerobic state transiently induces the major heat-shock proteins (at 68 and 89 kDa). As the period of anaerobiosis increases, this heat-shock induction disappears and a new set of proteins (at 76 and 97 kDa) is induced. By two-dimensional gel electrophoresis and partial proteolytic mapping, these new proteins, which are induced by anaerobic exposures exceeding 12 hr, are identical to 76 and 97 kDa (p76 and p97, respectively) proteins induced by extended periods of glucose deprivation (greater than 14 hr) when oxygen is present. Furthermore, the induction of these proteins under anoxia occurs in the presence of glucose, and increasing the glucose content of the starting media does not affect the induction. When anaerobic p76 and p97 induced cells are returned to atmospheric oxygen, p76 and p97 are repressed, while the heat-shock proteins are again transiently induced. This work further suggests the importance of deprivation and release environments in controlling the expression of these two stress protein systems. It is suggested that their natural expression may be determined by comparable circumstances.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Sciandra JJ,Subjeck JR,Hughes CS

doi

10.1073/pnas.81.15.4843

subject

Has Abstract

pub_date

1984-08-01 00:00:00

pages

4843-7

issue

15

eissn

0027-8424

issn

1091-6490

journal_volume

81

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Activation of Na+/H+ exchange in cultured fibroblasts: synergism and antagonism between phorbol ester, Ca2+ ionophore, and growth factors.

    abstract：:The effects of phorbol 12-myristate 13-acetate (PMA), a potent activator of protein kinase C, on Na+ influx were investigated in cultured human foreskin fibroblasts (HSWP cells). We report here that in serum-deprived HSWP cells the addition of PMA alone has no significant effect on Na+ influx. However, the addition of...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.23.8053

    authors： Vicentini LM,Villereal ML

    更新日期：1985-12-01 00:00:00

  • Discovery and analysis of the first endogenous lentivirus.

    abstract：:The lentiviruses are associated with a wide range of chronic diseases in mammals. These include immunodeficiencies (such as HIV/AIDS in humans), malignancies, and lymphatic and neurological disorders in primates, felids, and a variety of wild and domesticated ungulates. Evolutionary analyses of the genomic sequences o...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0700471104

    authors： Katzourakis A,Tristem M,Pybus OG,Gifford RJ

    更新日期：2007-04-10 00:00:00

  • TRPM8 activation attenuates inflammatory responses in mouse models of colitis.

    abstract：:Transient Receptor Potential Melastatin-8 (TRPM8), a recently identified member of the transient receptor potential (TRP) family of ion channels, is activated by mild cooling and by chemical compounds such as the supercooling agent, icilin. Since cooling, possibly involving TRPM8 stimulation, diminishes injury-induced...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1217431110

    authors： Ramachandran R,Hyun E,Zhao L,Lapointe TK,Chapman K,Hirota CL,Ghosh S,McKemy DD,Vergnolle N,Beck PL,Altier C,Hollenberg MD

    更新日期：2013-04-30 00:00:00

  • Anti-CD47 antibody-mediated phagocytosis of cancer by macrophages primes an effective antitumor T-cell response.

    abstract：:Mobilization of the T-cell response against cancer has the potential to achieve long-lasting cures. However, it is not known how to harness antigen-presenting cells optimally to achieve an effective antitumor T-cell response. In this study, we show that anti-CD47 antibody-mediated phagocytosis of cancer by macrophages...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1305569110

    authors： Tseng D,Volkmer JP,Willingham SB,Contreras-Trujillo H,Fathman JW,Fernhoff NB,Seita J,Inlay MA,Weiskopf K,Miyanishi M,Weissman IL

    更新日期：2013-07-02 00:00:00

  • Conserved gene regulatory module specifies lateral neural borders across bilaterians.

    abstract：:The lateral neural plate border (NPB), the neural part of the vertebrate neural border, is composed of central nervous system (CNS) progenitors and peripheral nervous system (PNS) progenitors. In invertebrates, PNS progenitors are also juxtaposed to the lateral boundary of the CNS. Whether there are conserved molecula...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1704194114

    authors： Li Y,Zhao D,Horie T,Chen G,Bao H,Chen S,Liu W,Horie R,Liang T,Dong B,Feng Q,Tao Q,Liu X

    更新日期：2017-08-01 00:00:00

  • Determining complete electron flow in the cofactor photoreduction of oxidized photolyase.

    abstract：:The flavin cofactor in photoenzyme photolyase and photoreceptor cryptochrome may exist in an oxidized state and should be converted into reduced state(s) for biological functions. Such redox changes can be efficiently achieved by photoinduced electron transfer (ET) through a series of aromatic residues in the enzyme. ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1311073110

    authors： Liu Z,Tan C,Guo X,Li J,Wang L,Sancar A,Zhong D

    更新日期：2013-08-06 00:00:00

  • Favorable outcome of experimental islet xenotransplantation without immunosuppression in a nonhuman primate model of diabetes.

    abstract：:Transplantation of pancreatic islets for treating type 1 diabetes is restricted to patients with critical metabolic lability resulting from the need for immunosuppression and the shortage of donor organs. To overcome these barriers, we developed a strategy to macroencapsulate islets from different sources that allow t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1708420114

    authors： Ludwig B,Ludwig S,Steffen A,Knauf Y,Zimerman B,Heinke S,Lehmann S,Schubert U,Schmid J,Bleyer M,Schönmann U,Colton CK,Bonifacio E,Solimena M,Reichel A,Schally AV,Rotem A,Barkai U,Grinberg-Rashi H,Kaup FJ,Avni Y,J

    更新日期：2017-10-31 00:00:00

  • Crystal structures of an archaeal oligosaccharyltransferase provide insights into the catalytic cycle of N-linked protein glycosylation.

    abstract：:Oligosaccharyltransferase transfers an oligosaccharide chain to the asparagine residues in proteins. The archaeal and eubacterial oligosaccharyltransferases are single subunit membrane enzymes, referred to as "AglB" (archaeal glycosylation B) and "PglB" (protein glycosylation B), respectively. Only one crystal structu...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1309777110

    authors： Matsumoto S,Shimada A,Nyirenda J,Igura M,Kawano Y,Kohda D

    更新日期：2013-10-29 00:00:00

  • Prestimulus influences on auditory perception from sensory representations and decision processes.

    abstract：:The qualities of perception depend not only on the sensory inputs but also on the brain state before stimulus presentation. Although the collective evidence from neuroimaging studies for a relation between prestimulus state and perception is strong, the interpretation in the context of sensory computations or decision...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1524087113

    authors： Kayser SJ,McNair SW,Kayser C

    更新日期：2016-04-26 00:00:00

  • Arrestin-related proteins mediate pH signaling in fungi.

    abstract：:Metazoan arrestins bind to seven-transmembrane (7TM) receptors to regulate function. Aspergillus nidulans PalF, a protein involved in the fungal ambient pH signaling pathway, contains arrestin N-terminal and C-terminal domains and binds strongly to two different regions within the C-terminal cytoplasmic tail of the 7T...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0504776102

    authors： Herranz S,Rodríguez JM,Bussink HJ,Sánchez-Ferrero JC,Arst HN Jr,Peñalva MA,Vincent O

    更新日期：2005-08-23 00:00:00

  • TNFalpha induces ABCA1 through NF-kappaB in macrophages and in phagocytes ingesting apoptotic cells.

    abstract：:Recent evidence suggests that tumor necrosis factor alpha (TNFalpha) signaling in vascular cells can have antiatherogenic consequences, but the mechanisms are poorly understood. TNFalpha is released by free cholesterol-loaded apoptotic macrophages, and the clearance of these cells by phagocytic macrophages may help to...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0510345103

    authors： Gerbod-Giannone MC,Li Y,Holleboom A,Han S,Hsu LC,Tabas I,Tall AR

    更新日期：2006-02-28 00:00:00

  • Sequence analysis of carcinoembryonic antigen: identification of glycosylation sites and homology with the immunoglobulin supergene family.

    abstract：:A direct method for the determination of N-linked glycosylation sites in highly glycosylated proteins is described. Carcinoembryonic antigen (CEA) and a nonspecific crossreacting antigen (NCA) were chemically deglycosylated, and peptide maps were prepared by reverse-phase HPLC. The peptides were sequenced on a gas-pha...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.4.920

    authors： Paxton RJ,Mooser G,Pande H,Lee TD,Shively JE

    更新日期：1987-02-01 00:00:00

  • Significance analysis of xMap cytokine bead arrays.

    abstract：:Highly multiplexed assays using antibody coated, fluorescent (xMap) beads are widely used to measure quantities of soluble analytes, such as cytokines and antibodies in clinical and other studies. Current analyses of these assays use methods based on standard curves that have limitations in detecting low or high abund...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1112599109

    authors： Won JH,Goldberger O,Shen-Orr SS,Davis MM,Olshen RA

    更新日期：2012-02-21 00:00:00

  • GABA exerts protective and regenerative effects on islet beta cells and reverses diabetes.

    abstract：:Type 1 diabetes (T1D) is an autoimmune disease characterized by insulitis and islet β-cell loss. Thus, an effective therapy may require β-cell restoration and immune suppression. Currently, there is no treatment that can achieve both goals efficiently. We report here that GABA exerts antidiabetic effects by acting on ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1102715108

    authors： Soltani N,Qiu H,Aleksic M,Glinka Y,Zhao F,Liu R,Li Y,Zhang N,Chakrabarti R,Ng T,Jin T,Zhang H,Lu WY,Feng ZP,Prud'homme GJ,Wang Q

    更新日期：2011-07-12 00:00:00

  • Temperature increase of 21st century mitigation scenarios.

    abstract：:Estimates of 21st Century global-mean surface temperature increase have generally been based on scenarios that do not include climate policies. Newly developed multigas mitigation scenarios, based on a wide range of modeling approaches and socioeconomic assumptions, now allow the assessment of possible impacts of clim...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0711129105

    authors： Van Vuuren DP,Meinshausen M,Plattner GK,Joos F,Strassmann KM,Smith SJ,Wigley TM,Raper SC,Riahi K,de la Chesnaye F,den Elzen MG,Fujino J,Jiang K,Nakicenovic N,Paltsev S,Reilly JM

    更新日期：2008-10-07 00:00:00

  • Diffusion and scaling during early embryonic pattern formation.

    abstract：:Development of spatial patterns in multicellular organisms depends on gradients in the concentration of signaling molecules that control gene expression. In the Drosophila embryo, Bicoid (Bcd) morphogen controls cell fate along 70% of the anteroposterior axis but is translated from mRNA localized at the anterior pole....

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0509483102

    authors： Gregor T,Bialek W,de Ruyter van Steveninck RR,Tank DW,Wieschaus EF

    更新日期：2005-12-20 00:00:00

  • Epinephrine-induced elevation of guanosine 3':5'-cyclic monophosphate in isolated fat cells of rat.

    abstract：:The effects of epinephrine (as low as 0.1 muM) on guanosine 3':5'-cyclic monophosphate (cGMP) and adenosine 3':5'-cyclic monophosphate (cAMP) in isolated fat cells were examined. Epinephrine increased both cGMP and cAMP levels, with the elevation of cAMP preceding the rise of cGMP. Maximal elevation was obtained with ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.75.6.2684

    authors： Asakawa T,Ruiz J,Ho RJ

    更新日期：1978-06-01 00:00:00

  • Synthesis and bioassay of improved mosquito repellents predicted from chemical structure.

    abstract：:Mosquito repellency data on acylpiperidines derived from the U.S. Department of Agriculture archives were modeled by using molecular descriptors calculated by CODESSA PRO software. An artificial neural network model was developed for the correlation of these archival results and used to predict the repellent activity ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0800571105

    authors： Katritzky AR,Wang Z,Slavov S,Tsikolia M,Dobchev D,Akhmedov NG,Hall CD,Bernier UR,Clark GG,Linthicum KJ

    更新日期：2008-05-27 00:00:00

  • P-hacking in clinical trials and how incentives shape the distribution of results across phases.

    abstract：:Clinical research should conform to high standards of ethical and scientific integrity, given that human lives are at stake. However, economic incentives can generate conflicts of interest for investigators, who may be inclined to withhold unfavorable results or even tamper with data in order to achieve desired outcom...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1919906117

    authors： Adda J,Decker C,Ottaviani M

    更新日期：2020-06-16 00:00:00

  • Long-range compaction and flexibility of interphase chromatin in budding yeast analyzed by high-resolution imaging techniques.

    abstract：:Little is known about how chromatin folds in its native state. Using optimized in situ hybridization and live imaging techniques have determined compaction ratios and fiber flexibility for interphase chromatin in budding yeast. Unlike previous studies, ours examines nonrepetitive chromatin at intervals short enough to...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0402766101

    authors： Bystricky K,Heun P,Gehlen L,Langowski J,Gasser SM

    更新日期：2004-11-23 00:00:00

  • Estrogen receptor (ER)-beta isoforms: a key to understanding ER-beta signaling.

    abstract：:Estrogen receptor beta (ER-beta) regulates diverse physiological functions in the human body. Current studies are confined to ER-beta1, and the functional roles of isoforms 2, 4, and 5 remain unclear. Full-length ER-beta4 and -beta5 isoforms were obtained from a prostate cell line, and they exhibit differential expres...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0605676103

    authors： Leung YK,Mak P,Hassan S,Ho SM

    更新日期：2006-08-29 00:00:00

  • The mitochondrial ribosome of Xenopus laevis.

    abstract：:A mitochondrial ribosome with an approximate sedimentation coefficient of 60 S has been isolated from the ovary of the South African clawed toad, Xenopus laevis. This ribosome is active in a submitochondrial protein-synthesizing system. It contains two species of RNA, designated as "21S" and 13S RNA, which have sequen...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.66.1.117

    authors： Swanson RF,Dawid IB

    更新日期：1970-05-01 00:00:00

  • Community-based conservation in a globalized world.

    abstract：:Communities have an important role to play in biodiversity conservation. However, community-based conservation as a panacea, like government-based conservation as a panacea, ignores the necessity of managing commons at multiple levels, with vertical and horizontal interplay among institutions. The study of conservatio...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0702098104

    authors： Berkes F

    更新日期：2007-09-25 00:00:00

  • Distinct gene-specific mechanisms of arrhythmia revealed by cardiac gene transfer of two long QT disease genes, HERG and KCNE1.

    abstract：:The long QT syndrome (LQTS) is a heritable disorder that predisposes to sudden cardiac death. LQTS is caused by mutations in ion channel genes including HERG and KCNE1, but the precise mechanisms remain unclear. To clarify this situation we injected adenoviral vectors expressing wild-type or LQT mutants of HERG and KC...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.091239098

    authors： Hoppe UC,Marbán E,Johns DC

    更新日期：2001-04-24 00:00:00

  • Mutations in the bglY gene increase the frequency of spontaneous deletions in Escherichia coli K-12.

    abstract：:A strong mutator effect has been observed in Escherichia coli K-12 strains mutated in the bglY gene (27 min). The frequency of point mutations is not modified in bglY mutant strains. In contrast, a strong increase in spontaneous generation of large deletions has been observed in these strains, both for chromosomal mar...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.1.360

    authors： Lejeune P,Danchin A

    更新日期：1990-01-01 00:00:00

  • Interaction between amyloid precursor protein and presenilins in mammalian cells: implications for the pathogenesis of Alzheimer disease.

    abstract：:Mutations in the presenilin 1 (PS1) and presenilin 2 (PS2) genes increase the production of the highly amyloidogenic 42-residue form of amyloid beta-protein (Abeta42) in a variety of cell lines and transgenic mice. To elucidate the molecular mechanism of this effect, wild-type (wt) or mutant PS1 and PS2 genes were sta...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.15.8208

    authors： Xia W,Zhang J,Perez R,Koo EH,Selkoe DJ

    更新日期：1997-07-22 00:00:00

  • Characterization of individual polynucleotide molecules using a membrane channel.

    abstract：:We show that an electric field can drive single-stranded RNA and DNA molecules through a 2.6-nm diameter ion channel in a lipid bilayer membrane. Because the channel diameter can accommodate only a single strand of RNA or DNA, each polymer traverses the membrane as an extended chain that partially blocks the channel. ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.24.13770

    authors： Kasianowicz JJ,Brandin E,Branton D,Deamer DW

    更新日期：1996-11-26 00:00:00

  • Constitutively high dNTP concentration inhibits cell cycle progression and the DNA damage checkpoint in yeast Saccharomyces cerevisiae.

    abstract：:In eukaryotic cells the concentration of dNTP is highest in S phase and lowest in G1 phase and is controlled by ribonucleotide reductase (RNR). RNR activity is eliminated in all eukaryotes in G1 phase by a variety of mechanisms: transcriptional regulation, small inhibitory proteins, and protein degradation. After acti...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0610585104

    authors： Chabes A,Stillman B

    更新日期：2007-01-23 00:00:00

  • Exceptionally stable, redox-active supramolecular protein assemblies with emergent properties.

    abstract：:The designed assembly of proteins into well-defined supramolecular architectures not only tests our understanding of protein-protein interactions, but it also provides an opportunity to tailor materials with new physical and chemical properties. Previously, we described that RIDC3, a designed variant of the monomeric ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1319866111

    authors： Brodin JD,Carr JR,Sontz PA,Tezcan FA

    更新日期：2014-02-25 00:00:00

  • Development of potent gastrin-releasing peptide antagonists having a D-Pro-psi(CH2NH)-Phe-NH2 C terminus.

    abstract：:Gastrin-releasing peptide (GRP) is a 27-amino acid neuroendocrine hormone that may play a role in the pathophysiology of small cell lung carcinoma. GRP and bombesin, a structurally related peptide, stimulate the growth of some cultured cell types. C-terminal GRP peptide analogs were developed that inhibited 6 nM bombe...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.5.1922

    authors： Leban JJ,Kull FC Jr,Landavazo A,Stockstill B,McDermed JD

    更新日期：1993-03-01 00:00:00