Constitutively high dNTP concentration inhibits cell cycle progression and the DNA damage checkpoint in yeast Saccharomyces cerevisiae.

abstract：:The DNA strand exchange protein RAD51 facilitates the central step in homologous recombination, a process fundamentally important for accurate repair of damaged chromosomes, restart of collapsed replication forks, and telomere maintenance. The active form of RAD51 is a nucleoprotein filament that assembles on single-s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Subramanyam S,Ismail M,Bhattacharya I,Spies M

更新日期：2016-10-11 00:00:00

abstract：:Temperature lability of ADP-glucose pyrophosphorylase (AGP; glucose-1-phosphate adenylyltransferase; ADP: alpha-D-glucose-1-phosphate adenylyltransferase, EC 2.7.7.27), a key starch biosynthetic enzyme, may play a significant role in the heat-induced loss in maize seed weight and yield. Here we report the isolation an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Greene TW,Hannah LC

更新日期：1998-10-27 00:00:00

abstract：:The heat shock protein (Hsp)70 family of molecular chaperones interacts with unfolded proteins through a C-terminal substrate-binding domain (SBD) that is controlled by nucleotide binding to the N-terminal domain. The ATPase cycle is regulated by cochaperones, including DnaJ proteins that accelerate ATP hydrolysis to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Awad W,Estrada I,Shen Y,Hendershot LM

更新日期：2008-01-29 00:00:00

abstract：:We identified the phosphatidylinositol transfer protein (PITP) as being responsible for a powerful latent, nucleotide-independent, Golgi-vesiculating activity that is present in the cytosol but is only manifested as an uncontrolled activity in a cytosolic protein subfraction, in which it is separated from regulatory c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Simon JP,Morimoto T,Bankaitis VA,Gottlieb TA,Ivanov IE,Adesnik M,Sabatini DD

更新日期：1998-09-15 00:00:00

abstract：:During latent infection of humans with Mycobacterium tuberculosis, bacteria persist in the asymptomatic host within granulomas, organized collections of differentiated macrophages, and other immune cells. The mechanisms for persistence remain poorly understood, as is the metabolic and replicative state of the microbes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chan K,Knaak T,Satkamp L,Humbert O,Falkow S,Ramakrishnan L

更新日期：2002-03-19 00:00:00

abstract：:Noncompetitive inhibitors of the nicotinic acetylcholine (ACh) receptors suppress cation flux directly by binding in and blocking the open channel or indirectly by stabilizing closed states of the receptor. The lidocaine derivative QX-314 and the acridine derivative quinacrine act directly as open channel blockers, bu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu Y,Shi L,Karlin A

更新日期：2003-04-01 00:00:00

abstract：:Subunits A and B of chloroplast glyceraldehyde-3-phosphate dehydrogenase are synthesized as higher molecular weight precursors when polyadenylylated mRNA from angiosperm seedlings is translated in vitro by wheat germ ribosomes. The in vivo levels of mRNA coding for these precursors are strongly light dependent, and th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cerff R,Kloppstech K

更新日期：1982-12-01 00:00:00

abstract：:Natural killer (NK) cells mediate vital control of cancer and viral infection. They rely on MHC class I (MHC I)-specific self-receptors to identify and lyse diseased cells without harming self-MHC I-bearing host cells. NK cells bearing inhibitory self-receptors for host MHC I also undergo education, referred to as lic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gamache A,Cronk JM,Nash WT,Puchalski P,Gillespie A,Wei H,Gray L,Hammarskjold ML,Xu W,Brown MG

更新日期：2019-12-16 00:00:00

abstract：:Despite the proclamation of Lowenstam and Weiner that crustaceans are the "champions of mineral mobilization and deposition of the animal kingdom," relatively few proteins from the two main calcification sites in these animals, i.e., the exoskeleton and the transient calcium storage organs, have been identified, seque...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shechter A,Glazer L,Cheled S,Mor E,Weil S,Berman A,Bentov S,Aflalo ED,Khalaila I,Sagi A

更新日期：2008-05-20 00:00:00

abstract：:Investigation of retinal neurochemistry in a well-defined chick model of form-deprivation myopia indicated that dopamine and its metabolite 3,4-dihydroxyphenylacetic acid are reduced in myopic as compared to control eyes. The reduction in retinal dopamine is evident only during light adaptation and is accompanied by a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stone RA,Lin T,Laties AM,Iuvone PM

更新日期：1989-01-01 00:00:00

abstract：:The suppressor of Hairy-wing [su(Hw)] protein exerts a polar effect on gene expression by repressing the function of transcriptional enhancers located distally from the promoter with respect to the location of su(Hw) binding sequences. The directionality of this effect suggests that the su(Hw) protein specifically int...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Georgiev PG,Corces VG

更新日期：1995-05-23 00:00:00

abstract：:Purified fractions of human apotransferrin, monoferric transferrins with iron on the acid-labile binding site and on the acid-stable binding site, and diferric transferrin have been prepared. The iron loading and unloading behavior of these preparations has been examined by isoelectric focusing. Iron release from the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huebers H,Josephson B,Huebers E,Csiba E,Finch C

更新日期：1981-04-01 00:00:00

abstract：:By analogy with receptor-mediated endocytosis, clathrin-coated membrane segments at locations other than at the plasma membrane have been implicated in intracellular membrane transport/sorting. The crucial role of the Golgi apparatus in these processes prompted us to study the distribution at this level of clathrin-im...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Orci L,Ravazzola M,Amherdt M,Louvard D,Perrelet A

更新日期：1985-08-01 00:00:00

abstract：:Mobilization of the T-cell response against cancer has the potential to achieve long-lasting cures. However, it is not known how to harness antigen-presenting cells optimally to achieve an effective antitumor T-cell response. In this study, we show that anti-CD47 antibody-mediated phagocytosis of cancer by macrophages...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tseng D,Volkmer JP,Willingham SB,Contreras-Trujillo H,Fathman JW,Fernhoff NB,Seita J,Inlay MA,Weiskopf K,Miyanishi M,Weissman IL

更新日期：2013-07-02 00:00:00

abstract：:Behavioral sensitization of the gill-withdrawal reflex of Aplysia is caused by presynaptic facilitation at the synapses of the mechanoreceptor sensory neurons of the reflex onto the motor neurons and interneurons. The presynaptic facilitation has been shown to be simulated by serotonin (the putative presynaptic facili...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Klein M,Kandel ER

更新日期：1980-11-01 00:00:00

abstract：:Peripheral nerve injury induces a robust proregenerative program that drives axon regeneration. While many regeneration-associated genes are known, the mechanisms by which injury activates them are less well-understood. To identify such mechanisms, we performed a loss-of-function pharmacological screen in cultured adu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karney-Grobe S,Russo A,Frey E,Milbrandt J,DiAntonio A

更新日期：2018-10-16 00:00:00

abstract：:C1q-like genes (C1ql1-C1ql4) encode small, secreted proteins that are expressed in differential patterns in the brain but whose receptors and functions remain unknown. BAI3 protein, in contrast, is a member of the cell-adhesion class of G protein-coupled receptors that are expressed at high levels in the brain but who...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bolliger MF,Martinelli DC,Südhof TC

更新日期：2011-02-08 00:00:00

abstract：:Circadian rhythms in metazoan eukaryotes are controlled by an endogenous molecular clock. It functions in many locations, including subsets of brain neurons (clock neurons) within the central nervous system. Although the molecular clock relies on transcription/translation feedback loops, posttranscriptional regulation...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen X,Rosbash M

更新日期：2016-05-24 00:00:00

abstract：:Much of the success of Plasmodium falciparum in establishing persistent infections is attributed to immune evasion through antigenic variation. This process involves periodically exchanging variants of the major surface antigen PfEMP1, a protein also responsible for parasite cytoadherence. PfEMP1 is encoded by genes o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ralph SA,Scheidig-Benatar C,Scherf A

更新日期：2005-04-12 00:00:00

abstract：:Turkey erythrocyte membranes showed specific binding of [(3)H]epinephrine. The concentration of hormone required for half-maximal binding (30 muM) was the same as that required for half-maximal activation of the adenylate cyclase located in the same membrane preparation. The binding reaction at 37 degrees C reached co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schramm M,Feinstein H,Naim E,Lang E,Lasser M

更新日期：1972-02-01 00:00:00

abstract：:Speech recognition involves three processes: extraction of acoustic indices from the speech signal, estimation of the probability that the observed index string was caused by a hypothesized utterance segment, and determination of the recognized utterance via a search among hypothesized alternatives. This paper is not ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jelinek F

更新日期：1995-10-24 00:00:00

abstract：:Freezing avoidance conferred by different types of antifreeze proteins in various polar and subpolar fishes represents a remarkable example of cold adaptation, but how these unique proteins arose is unknown. We have found that the antifreeze glycoproteins (AFGPs) of the predominant Antarctic fish taxon, the notothenio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen L,DeVries AL,Cheng CH

更新日期：1997-04-15 00:00:00

abstract：:The expression and integration of membrane proteins into vesicle membranes is a critical step in the design of cell-mimetic biosensors, bioreactors, and artificial cells. While membrane proteins have been integrated into a variety of nonnatural membranes, the effects of the chemical and physical properties of these ve...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jacobs ML,Boyd MA,Kamat NP

更新日期：2019-03-05 00:00:00

abstract：:We used integrin alphaLbeta2 heterodimers containing I domains locked open (active) or closed (inactive) with disulfide bonds to investigate regulatory interactions among domains in integrins. mAbs to the alphaL I domain and beta2 I-like domain inhibit adhesion of wild-type alphaLbeta2 to intercellular adhesion molecu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu C,Shimaoka M,Zang Q,Takagi J,Springer TA

更新日期：2001-02-27 00:00:00

abstract：:ADP activates a family of cell surface receptors that modulate signaling pathways in a broad range of cells. ADP receptor antagonists are widely used to treat cardiovascular disease states. These studies identify a critical role for the stable reactive oxygen species hydrogen peroxide (H2O2) in mediating cellular resp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kalwa H,Sartoretto JL,Martinelli R,Romero N,Steinhorn BS,Tao M,Ozaki CK,Carman CV,Michel T

更新日期：2014-03-04 00:00:00

abstract：:Various compounds that affect signal transduction regulate the relative utilization of alternative processing pathways for the beta-amyloid precursor protein (beta APP) in intact cells, increasing the production of nonamyloidogenic soluble beta APP (s beta APP) and decreasing that of amyloidogenic beta-amyloid peptide...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu H,Sweeney D,Greengard P,Gandy S

更新日期：1996-04-30 00:00:00

abstract：:Coherent multidimensional electronic spectroscopy is commonly used to investigate photophysical phenomena such as light harvesting in photosynthesis in which the system returns back to its ground state after energy transfer. By contrast, we introduce multidimensional spectroscopy to study ultrafast photochemical proce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ruetzel S,Diekmann M,Nuernberger P,Walter C,Engels B,Brixner T

更新日期：2014-04-01 00:00:00

abstract：:We show in climate model experiments that large-scale afforestation in northern mid-latitudes warms the Northern Hemisphere and alters global circulation patterns. An expansion of dark forests increases the absorption of solar energy and increases surface temperature, particularly in regions where the land surface is ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Swann AL,Fung IY,Chiang JC

更新日期：2012-01-17 00:00:00

abstract：:Studies of biotic remains of polar ice caps have been limited to morphological identification of plant pollen and spores. By using sensitive molecular techniques, we now demonstrate a much greater range of detectable organisms; from 2000- and 4000-year-old ice-core samples, we obtained and characterized 120 clones tha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Willerslev E,Hansen AJ,Christensen B,Steffensen JP,Arctander P

更新日期：1999-07-06 00:00:00

abstract：:The DNA-binding protein CCCTC-binding factor (CTCF) and the cohesin complex function together to shape chromatin architecture in mammalian cells, but the molecular details of this process remain unclear. Here, we demonstrate that a 79-aa region within the CTCF N terminus is essential for cohesin positioning at CTCF bi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pugacheva EM,Kubo N,Loukinov D,Tajmul M,Kang S,Kovalchuk AL,Strunnikov AV,Zentner GE,Ren B,Lobanenkov VV

更新日期：2020-01-28 00:00:00