Multidimensional spectroscopy of photoreactivity.

abstract：:Synaptic transmission of the light response from photoreceptors to second-order cells of the retina was studied with the whole-cell patch-clamp technique in tiger salamander (Ambystoma tigrinum) retinal slices. Synaptic strength is modulated by extracellular pH in a striking manner: Light-sensitive postsynaptic curren...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barnes S,Merchant V,Mahmud F

更新日期：1993-11-01 00:00:00

abstract：:The Caulobacter crescentus flagellum is formed at a specific time in the cell cycle and its assembly requires the ordered expression of a large number of genes. These genes are controlled in a positive trans-acting hierarchy that reflects the order of assembly of the flagellum. Using plasmids carrying transcriptional ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu H,Dingwall A,Shapiro L

更新日期：1989-09-01 00:00:00

abstract：:To improve the efficacy of plasminogen activators, we produced a monoclonal antibody (RWR) that inhibits human alpha 2-antiplasmin (alpha 2AP). In addition to inhibiting alpha 2AP in plasma, RWR binds to and inhibits fibrin cross-linked alpha 2AP and reproduces the "spontaneous" clot lysis that is the hallmark of huma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reed GL 3rd,Matsueda GR,Haber E

更新日期：1990-02-01 00:00:00

abstract：:This paper describes the use of laminar flow of liquids in capillary systems to pattern the cell culture substrate, to perform patterned cell deposition, and to pattern the cell culture media. We demonstrate the patterning of the cell culture substrate with different proteins, the patterning of different types of cell...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Takayama S,McDonald JC,Ostuni E,Liang MN,Kenis PJ,Ismagilov RF,Whitesides GM

更新日期：1999-05-11 00:00:00

abstract：:Clonal deletion provides an important mechanism for the elimination of autoreactive T cells. Deletion is accomplished by programmed cell death directed by interaction of the T-cell receptor (TCR) of the developing thymocyte with major histocompatibility complex elements in the thymic environment. In this report we pre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Russell JH,White CL,Loh DY,Meleedy-Rey P

更新日期：1991-03-15 00:00:00

abstract：:The maytansinoid drug DM1 is 100- to 1000-fold more cytotoxic than anticancer drugs that are currently in clinical use. The immunoconjugate C242-DM1 was prepared by conjugating DM1 to the monoclonal antibody C242, which recognizes a mucin-type glycoprotein expressed to various extents by human colorectal cancers. C242...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu C,Tadayoni BM,Bourret LA,Mattocks KM,Derr SM,Widdison WC,Kedersha NL,Ariniello PD,Goldmacher VS,Lambert JM,Blättler WA,Chari RV

更新日期：1996-08-06 00:00:00

abstract：:Dual-color fluorescence cross-correlation spectroscopy (dual-color FCS) has previously been shown to be a suitable tool not only for binding but also for catalytic rate studies. In this work, its application as a rapid method for high-throughput screening (HTS) and evolutionary biotechnology is described. This applica...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koltermann A,Kettling U,Bieschke J,Winkler T,Eigen M

更新日期：1998-02-17 00:00:00

abstract：:Fibroblast growth factor 1 (FGF-1) is a potent angiogenic and neurotrophic factor whose structure lacks a classical signal sequence for secretion. Although the initiation of these biological activities involves the interaction between FGF-1 and cell surface receptors, the mechanism responsible for the regulation of FG...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jackson A,Friedman S,Zhan X,Engleka KA,Forough R,Maciag T

更新日期：1992-11-15 00:00:00

abstract：:We give a functional generalization of fractal scaling laws applied to response problems as well as to probability distributions. We consider excitations and responses, which are functions of a given state vector. Based on scaling arguments, we derive a general nonlinear response functional scaling law, which expresse...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vlad MO,Morán F,Popa VT,Szedlacsek SE,Ross J

更新日期：2007-03-20 00:00:00

abstract：:We describe a method that combines two- and three-color single-molecule FRET spectroscopy with 2D FRET efficiency-lifetime analysis to probe the oligomerization process of intrinsically disordered proteins. This method is applied to the oligomerization of the tetramerization domain (TD) of the tumor suppressor protein...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chung HS,Meng F,Kim JY,McHale K,Gopich IV,Louis JM

更新日期：2017-08-15 00:00:00

abstract：:ATM (ataxia-telangiectasia mutated) and ATR (ATM and Rad3-related) are large PI3 kinases whose human mutations result in complex syndromes that include a compromised DNA damage response (DDR) and prominent nervous system phenotypes. Both proteins are nuclear-localized in keeping with their DDR functions, yet both are ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheng A,Zhao T,Tse KH,Chow HM,Cui Y,Jiang L,Du S,Loy MMT,Herrup K

更新日期：2018-01-09 00:00:00

abstract：:Salivary gland cells in the larvae of the dipteran Chironomus tentans offer unique possibilities to visualize the assembly and nucleocytoplasmic transport of a specific transcription product. Each nucleus harbors four giant polytene chromosomes, whose transcription sites are expanded, or puffed. On chromosome IV, ther...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Daneholt B

更新日期：2001-06-19 00:00:00

abstract：:A single animal group can display different types of collective motion at different times. For a one-dimensional individual-based model of self-organizing group formation, we show that repeated switching between distinct ordered collective states can occur entirely because of stochastic effects. We introduce a framewo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kolpas A,Moehlis J,Kevrekidis IG

更新日期：2007-04-03 00:00:00

abstract：:The tumor suppressor protein p53 localizes to microtubules (MT) and, in response to DNA damage, is transported to the nucleus via the MT minus-end-directed motor protein dynein. Dynein is also responsible for MT-mediated nuclear targeting of adenovirus type 2 (Ad2). Here we show that treatment with low concentrations ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Giannakakou P,Nakano M,Nicolaou KC,O'Brate A,Yu J,Blagosklonny MV,Greber UF,Fojo T

更新日期：2002-08-06 00:00:00

abstract：:Despite the importance of Mg(2+) for numerous cellular activities, the mechanisms underlying its import and homeostasis are poorly understood. The CorA family is ubiquitous and is primarily responsible for Mg(2+) transport. However, the key questions-such as, the ion selectivity, the transport pathway, and the gating ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guskov A,Nordin N,Reynaud A,Engman H,Lundbäck AK,Jong AJ,Cornvik T,Phua T,Eshaghi S

更新日期：2012-11-06 00:00:00

abstract：:The antigenic mosaic of the Archaebacterium Methanobacterium thermoautotrophicum, strain delta H, was analyzed with a panel of six monoclonal antibodies. Five antigenic determinants were identified. One contains N-acetyl-D-glucosamine, another contains N-acetyl-D-galactosamine, and a third contains gamma-glutamylalani...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Conway de Macario E,Macario AJ,Magariños MC,König H,Kandler O

更新日期：1983-10-01 00:00:00

abstract：:The mechanistic target of rapamycin complex 1 (mTORC1) growth pathway detects nutrients through a variety of sensors and regulators that converge on the Rag GTPases, which form heterodimers consisting of RagA or RagB tightly bound to RagC or RagD and control the subcellular localization of mTORC1. The Rag heterodimer ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shen K,Sabatini DM

更新日期：2018-09-18 00:00:00

abstract：:Using the technique of DNA fingerprinting, we investigated the genetic structure within and among four wild-caught colonies (n = 50 individuals) of a eusocial mammal, the naked mole-rat (Heterocephalus glaber; Rodentia: Bathyergidae). We found that DNA fingerprints of colony-mates were strikingly similar and that betw...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reeve HK,Westneat DF,Noon WA,Sherman PW,Aquadro CF

更新日期：1990-04-01 00:00:00

abstract：:Transcription factors (TFs) are the main players in transcriptional regulation in eukaryotes. However, it remains unclear what role TFs played in the origin of all of the different eukaryotic multicellular lineages. In this paper, we explore how the origin of TF repertoires shaped eukaryotic evolution and, in particul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： de Mendoza A,Sebé-Pedrós A,Šestak MS,Matejcic M,Torruella G,Domazet-Loso T,Ruiz-Trillo I

更新日期：2013-12-10 00:00:00

abstract：:After collision with their host cells, virus particles may remain mobile on cell surfaces until they become attached at firm binding sites. We propose that a virion will arrive within a typical median time at such a site, generating a membrane signal such as an increased membrane fluorescence in cells labeled with the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bayer ME,Bayer MH

更新日期：1981-09-01 00:00:00

abstract：:The spread of bacteria resistant to antimicrobial agents calls for population-wide treatment strategies to delay or reverse the trend toward antibiotic resistance. Here we propose new criteria for the evaluation of the population-wide effects of treatment protocols for directly transmitted bacterial infections and dis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bonhoeffer S,Lipsitch M,Levin BR

更新日期：1997-10-28 00:00:00

abstract：:Sufficient conditions are found for a biinvariant operator on a compact Lie group to be bounded on L(p), 1 < p < infinity. The proof uses properties of g-functions on such a group, and an analog to the familiar relationship between differentiation and multiplication under the Fourier transform. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weiss NJ

更新日期：1971-05-01 00:00:00

abstract：:Fusions of the lac operon to genes induced by treatment with sublethal levels of alkylating agents have been selected from random insertions of the Mu-dl(ApRlac) phage by screening for induction of beta-galactosidase activity in the presence of methyl methanesulfonate. Genetic analysis reveals that these fusions resul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Volkert MR,Nguyen DC

更新日期：1984-07-01 00:00:00

abstract：:Poly(ADP ribose) polymerase inhibitors (PARPi) have efficacy in triple negative breast (TNBC) and ovarian cancers (OCs) harboring BRCA mutations, generating homologous recombination deficiencies (HRDs). DNA methyltransferase inhibitors (DNMTi) increase PARP trapping and reprogram the DNA damage response to generate HR...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McLaughlin LJ,Stojanovic L,Kogan AA,Rutherford JL,Choi EY,Yen RC,Xia L,Zou Y,Lapidus RG,Baylin SB,Topper MJ,Rassool FV

更新日期：2020-07-28 00:00:00

abstract：:Rare high-3He/4He signatures in ocean island basalts (OIB) erupted at volcanic hotspots derive from deep-seated domains preserved in Earth's interior. Only high-3He/4He OIB exhibit anomalous 182W-an isotopic signature inherited during the earliest history of Earth-supporting an ancient origin of high 3He/4He. However,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jackson MG,Blichert-Toft J,Halldórsson SA,Mundl-Petermeier A,Bizimis M,Kurz MD,Price AA,Harðardóttir S,Willhite LN,Breddam K,Becker TW,Fischer RA

更新日期：2020-12-08 00:00:00

abstract：:Two mouse monoclonal anti-anti-idiotopic antibodies (anti-anti-Id, Ab3), AF14 and AF52, were prepared by immunizing BALB/c mice with rabbit polyclonal anti-idiotypic antibodies (anti-Id, Ab2) raised against antibody D1.3 (Ab1) specific for the antigen hen egg lysozyme. AF14 and AF52 react with an "internal image" mono...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldbaum FA,Velikovsky CA,Dall'Acqua W,Fossati CA,Fields BA,Braden BC,Poljak RJ,Mariuzza RA

更新日期：1997-08-05 00:00:00

abstract：:The x-ray crystal structure of an oligomeric miniprotein has been determined to a 1.2-A resolution by means of multiwavelength anomalous diffraction phasing with selenomethionine analogs that retain the biophysical characteristics of the native peptide. Peptide 1, comprising alpha and beta secondary structure elements...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ali MH,Peisach E,Allen KN,Imperiali B

更新日期：2004-08-17 00:00:00

abstract：:Although proteins generally fold to their thermodynamically most stable state, some metastable proteins populate higher free energy states. Conformational changes from metastable higher free energy states to lower free energy states with greater stability can then generate the work required to perform physiologically ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chandrasekhar K,Ke H,Wang N,Goodwin T,Gierasch LM,Gershenson A,Hebert DN

更新日期：2016-06-07 00:00:00

abstract：:Humans' remarkable ability to quickly and accurately discriminate among thousands of highly similar complex objects demands rapid and precise neural computations. To elucidate the process by which this is achieved, we used magnetoencephalography to measure spatiotemporal patterns of neural activity with high temporal ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vida MD,Nestor A,Plaut DC,Behrmann M

更新日期：2017-01-10 00:00:00

abstract：:The hormone gibberellin (GA) is a key regulator of plant growth. Many of the components of the gibberellin signal transduction [e.g., GIBBERELLIN INSENSITIVE DWARF 1 (GID1) and DELLA], biosynthesis [e.g., GA 20-oxidase (GA20ox) and GA3ox], and deactivation pathways have been identified. Gibberellin binds its receptor,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Middleton AM,Úbeda-Tomás S,Griffiths J,Holman T,Hedden P,Thomas SG,Phillips AL,Holdsworth MJ,Bennett MJ,King JR,Owen MR

更新日期：2012-05-08 00:00:00