Molecular cloning of the alpha-1 subunit of an omega-conotoxin-sensitive calcium channel.

abstract：:We are interested in using recombinant adeno-associated viral vectors in the treatment of hemophilia A. Because of the size constraints of recombinant adeno-associated viral vectors, we delivered the heavy and light chains of the human factor 8 (hFVIII) cDNA independently by using two separate vectors. Recombinant AAV...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Burton M,Nakai H,Colosi P,Cunningham J,Mitchell R,Couto L

更新日期：1999-10-26 00:00:00

abstract：:Neuromelanin (NM) isolated from the substantia nigra region of the human brain was studied by scanning probe and photoelectron emission microscopies. Atomic force microscopy reveals that NM granules are comprised of spherical structures with a diameter of approximately 30 nm, similar to that observed for Sepia cuttlef...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bush WD,Garguilo J,Zucca FA,Albertini A,Zecca L,Edwards GS,Nemanich RJ,Simon JD

更新日期：2006-10-03 00:00:00

abstract：:Most, if not all, peptide- and protein-based hydrogels formed by self-assembly can be characterized as kinetically trapped 3D networks of fibrils. The propensity of disease-associated amyloid-forming peptides and proteins to assemble into polymorphic fibrils suggests that cross-β fibrils comprising hydrogels may also ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nagy-Smith K,Moore E,Schneider J,Tycko R

更新日期：2015-08-11 00:00:00

abstract：:The spectrum of cellular targets in the autoimmune diseases is both large and varied and includes among the nuclear components the so-called Sm and RNP antigens associated with systemic lupus erythematosus. The use of immunoaffinity chromatography with dual specificity for the Sm and RNP antigens has allowed for their...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： White PJ,Gardner WD,Hoch SO

更新日期：1981-01-01 00:00:00

abstract：:In the presence of the S polypeptide, maize chloroplast DNA-dependent RNA polymerase preferentially transcribes sequences within the 2200-nucleotide-pair-long maize chloroplast chromosome fragment Eco [unk] from a supercoiled chimeric plasmid cloned in Escherichia coli [Jolly, S. O. & Bogorad, L. (1980) Proc. Natl. Ac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schwarz Z,Jolly SO,Steinmetz AA,Bogorad L

更新日期：1981-06-01 00:00:00

abstract：:Ras is a guanine nucleotide-binding protein that cycles between inactive GDP-bound and active GTP-bound states to regulate a diverse array of cellular processes, including cell growth, apoptosis, and differentiation. The guanine nucleotide-bound state of Ras is tightly maintained by regulatory factors to promote regul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williams JG,Pappu K,Campbell SL

更新日期：2003-05-27 00:00:00

abstract：:For 2 < k [abstract: see text] we define lifts of cuspidal Poincaré series in S(k)(Gamma(0)(N)) to weight 2 - k harmonic weak Maass forms. This construction answers a question of Dyson by providing the general framework "explaining" Ramanujan's mock theta functions. As an application, we show that the number of partit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bringmann K,Ono K

更新日期：2007-03-06 00:00:00

abstract：:In maturing T lineage cells, the helix-loop-helix protein E47 has been shown to enforce a critical proliferation and developmental checkpoint commonly referred to as beta selection. To examine how E47 regulates cellular expansion and developmental progression, we have used an E2A-deficient lymphoma cell line and DNA m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schwartz R,Engel I,Fallahi-Sichani M,Petrie HT,Murre C

更新日期：2006-06-27 00:00:00

abstract：:In this article we report an experiment that examines how demand-side bidding can discipline generators in a market for electric power. First we develop a treatment without demand-side bidding; two large firms are allocated baseload and intermediate cost generators such that either firm might unilaterally withhold the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rassenti SJ,Smith VL,Wilson BJ

更新日期：2003-03-04 00:00:00

abstract：:Saccharopolyspora erythraea makes erythromycin, an antibiotic commonly used in human medicine. Unusually, the erythromycin biosynthetic (ery) cluster lacks a pathway-specific regulatory gene. We isolated a transcriptional regulator of the ery biosynthetic genes from S. erythraea and found that this protein appears to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chng C,Lum AM,Vroom JA,Kao CM

更新日期：2008-08-12 00:00:00

abstract：:A 90-kDa surface glycoprotein was previously isolated and shown to be required for infection by the "major" group of human rhinovirus (HRV) serotypes. In the present work, the amino acid sequence of the receptor protein was obtained from CNBr and tryptic peptides. Using degenerate oligonucleotides predicted from the p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tomassini JE,Graham D,DeWitt CM,Lineberger DW,Rodkey JA,Colonno RJ

更新日期：1989-07-01 00:00:00

abstract：:Cotranslational folding (CTF) is a fundamental molecular process that ensures efficient protein biosynthesis and minimizes the formation of misfolded states. However, the complexity of this process makes it extremely challenging to obtain structural characterizations of CTF pathways. Here, we correlate observations of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Waudby CA,Wlodarski T,Karyadi ME,Cassaignau AME,Chan SHS,Wentink AS,Schmidt-Engler JM,Camilloni C,Vendruscolo M,Cabrita LD,Christodoulou J

更新日期：2018-09-25 00:00:00

abstract：:The free radicals nitric oxide and superoxide anion react to form peroxynitrite (ONOO-), a highly toxic oxidant species. In vivo formation of ONOO- has been demonstrated in shock and inflammation. Herein we provide evidence that cytotoxicity in cells exposed to ONOO- is mediated by DNA strand breakage and the subseque...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Szabó C,Zingarelli B,O'Connor M,Salzman AL

更新日期：1996-03-05 00:00:00

abstract：:Cortical networks that have been found to operate close to a critical point exhibit joint activations of large numbers of neurons. However, in motor cortex of the awake macaque monkey, we observe very different dynamics: massively parallel recordings of 155 single-neuron spiking activities show weak fluctuations on th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dahmen D,Grün S,Diesmann M,Helias M

更新日期：2019-06-25 00:00:00

abstract：:Genomic and cDNA clones for the human nerve growth factor receptor have been used in conjunction with somatic cell hybrid analysis and in situ hybridization to localize the nerve growth factor receptor locus to human chromosome region 17q12-q22. Additionally, part, if not all, of the nerve growth factor receptor locus...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huebner K,Isobe M,Chao M,Bothwell M,Ross AH,Finan J,Hoxie JA,Sehgal A,Buck CR,Lanahan A

更新日期：1986-03-01 00:00:00

abstract：:Studies of prostate cancer pathogenesis and development of new therapies have been hampered by a lack of appropriate mouse models. We have generated PSA-Cre-ER(T2) mice that express the tamoxifen-dependent Cre-ER(T2) recombinase selectively in prostatic epithelium, thus allowing us to target floxed genes selectively i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ratnacaram CK,Teletin M,Jiang M,Meng X,Chambon P,Metzger D

更新日期：2008-02-19 00:00:00

abstract：:A protein fraction from B. subtilis infected with phage SP01 (fraction LGG) stimulates the activity of RNA polymerase (EC 2.7.7.6; nucleosidetriphosphate:RNA nucleotidyltransferase) core from uninfected bacteria. Fraction LGG contains a protein (P-28, molecular weight 28,000) that is labeled after phage infection and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Duffy JJ,Petrusek RL,Geiduschek EP

更新日期：1975-06-01 00:00:00

abstract：:A major involvement of IFNalpha in the etiopathogenesis of systemic lupus erythematosus has been suggested by clinical observations, including the increase of serum levels of this cytokine in patients with active disease. Supporting this hypothesis, we have shown that expression of IFNalpha from a recombinant adenovir...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zagury D,Le Buanec H,Mathian A,Larcier P,Burnett R,Amoura Z,Emilie D,Peltre G,Bensussan A,Bizzini B,Gallo RC,Koutouzov S

更新日期：2009-03-31 00:00:00

abstract：:Undesired side products of DNA transfections are usually discarded. However, here, we show that such products may provide insight into mutational events that are also a major driving force in protein evolution. While studying the small heat-shock protein alphaA-crystallin, we transfected the hamster alphaA-crystallin ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： van Rijk AA,de Jong WW,Bloemendal H

更新日期：1999-07-06 00:00:00

abstract：:Nonresolving chronic inflammation at the neoplastic site is consistently associated with promoting tumor progression and poor patient outcomes. However, many aspects behind the mechanisms that establish this tumor-promoting inflammatory microenvironment remain undefined. Using bladder cancer (BC) as a model, we found ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheah MT,Chen JY,Sahoo D,Contreras-Trujillo H,Volkmer AK,Scheeren FA,Volkmer JP,Weissman IL

更新日期：2015-04-14 00:00:00

abstract：:To study the origin of and the degree of T-cell antigen receptor (TCR) diversity of Thy-1+ dendritic epidermal cells (Thy-1+ dECs) in mice, we have developed a monoclonal antibody (mAb 536) to the gamma delta TCR. mAb 536 binds to and stimulates interleukin 2 secretion from Thy-1+ dEC but not cells that express TCR co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Havran WL,Grell S,Duwe G,Kimura J,Wilson A,Kruisbeek AM,O'Brien RL,Born W,Tigelaar RE,Allison JP

更新日期：1989-06-01 00:00:00

abstract：:Lineage plasticity is a prominent feature of pancreatic ductal adenocarcinoma (PDA) cells, which can occur via deregulation of lineage-specifying transcription factors. Here, we show that the zinc finger protein ZBED2 is aberrantly expressed in PDA and alters tumor cell identity in this disease. Unexpectedly, our epig...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Somerville TDD,Xu Y,Wu XS,Maia-Silva D,Hur SK,de Almeida LMN,Preall JB,Koo PK,Vakoc CR

更新日期：2020-05-26 00:00:00

abstract：:Surfaces with patterned wettability contrast are important in industrial applications such as heat transfer, water collection, and particle separation. Traditional methods of fabricating such surfaces rely on microfabrication technologies, which are only applicable to certain substrates and are difficult to scale up a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen D,McKinley GH,Cohen RE

更新日期：2016-07-19 00:00:00

abstract：:Plant-made vaccines have been the subject of intense interest because they can be produced economically in large scale without the use of animal-derived components. Plant-made therapeutic vaccines against challenging chronic diseases, such as cancer, have received little research attention, and no previous human clini...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McCormick AA,Reddy S,Reinl SJ,Cameron TI,Czerwinkski DK,Vojdani F,Hanley KM,Garger SJ,White EL,Novak J,Barrett J,Holtz RB,Tusé D,Levy R

更新日期：2008-07-22 00:00:00

abstract：:The degradation of the plant cell wall by glycoside hydrolases is central to environmentally sustainable industries. The major polysaccharides of the plant cell wall are cellulose and xylan, a highly decorated β-1,4-xylopyranose polymer. Glycoside hydrolases displaying multiple catalytic functions may simplify the enz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McKee LS,Peña MJ,Rogowski A,Jackson A,Lewis RJ,York WS,Krogh KB,Viksø-Nielsen A,Skjøt M,Gilbert HJ,Marles-Wright J

更新日期：2012-04-24 00:00:00

abstract：:Antiviral and macrophage-priming activities in the supernatant medium of a subclone of a concanavalin A-stimulated mouse T-cell hybridoma were investigated. The two activities were associated with a molecular weight of approximately 50,000 and could not be separated by various approaches. Both activities were eliminat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pace JL,Russell SW,Schreiber RD,Altman A,Katz DH

更新日期：1983-06-01 00:00:00

abstract：:Crosses between closely related animal species often result in male hybrids that are sterile, and the molecular and functional basis of genetic factors for hybrid male sterility is of great interest. Here, we report a molecular and functional analysis of HMS1, a region of 9.2 kb in chromosome 3 of Drosophila mauritian...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liénard MA,Araripe LO,Hartl DL

更新日期：2016-07-19 00:00:00

abstract：:Previous studies have shown that Delta-mediated Notch signaling regulates the number of early differentiating neurons. However, the role of Notch activation and Jagged-mediated signaling during late neurogenesis remains poorly defined. In the developing spinal cord of zebrafish, GABAergic Kolmer-Agduhr (KA'') cells an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yeo SY,Chitnis AB

更新日期：2007-04-03 00:00:00

abstract：:An inwardly rectifying, ATP-regulated K+ channel with a distinctive molecular architecture, ROMK1, was recently cloned from rat kidney. Using patch clamp techniques, we have investigated the regulation of ROMK1 with particular emphasis on phosphorylation/dephosphorylation processes. Spontaneous channel rundown occurre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McNicholas CM,Wang W,Ho K,Hebert SC,Giebisch G

更新日期：1994-08-16 00:00:00

abstract：:Glial cells are traditionally regarded as elements for structural support and ionic homeostasis, but have recently attracted attention as putative integral elements of the machinery involved in synaptic transmission and plasticity. Here, we demonstrate that calcium-binding protein S100B, which is synthesized in consid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nishiyama H,Knopfel T,Endo S,Itohara S

更新日期：2002-03-19 00:00:00