Structural and biochemical studies of p21Ras S-nitrosylation and nitric oxide-mediated guanine nucleotide exchange.

abstract：:The phosphodiester bond formation by DNA-dependent RNA-polymerase (RNA nucleotidyltransferase, nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) can in principle result in retention, inversion, or racemization of configuration at the alpha-phosphorus of the nucleoside 5'-triphosphate being polymerized. As...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eckstein F,Armstrong VW,Sternbach H

更新日期：1976-09-01 00:00:00

abstract：:We describe the partial purification of an endonuclease from calf thymus that nicks phage PM2 DNA irradiated with UV doses producing only a few pyrimidine dimers per molecule. It has much less activity on DNA that has been subjected to enzymatic photoreactivation after UV irradiation. The calf thymus endonuclease is d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Waldstein EA,Peller S,Setlow RB

更新日期：1979-08-01 00:00:00

abstract：:An efficient technique has been developed for performing in vivo site-directed mutagenesis in Drosophila melanogaster. This procedure involves directed repair of P-element-induced DNA lesions after injection of a modified DNA sequence into early embryos. An oligonucleotide of 50 base pairs, whose sequence spans the P-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Banga SS,Boyd JB

更新日期：1992-03-01 00:00:00

abstract：:Mosquito repellency data on acylpiperidines derived from the U.S. Department of Agriculture archives were modeled by using molecular descriptors calculated by CODESSA PRO software. An artificial neural network model was developed for the correlation of these archival results and used to predict the repellent activity ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Katritzky AR,Wang Z,Slavov S,Tsikolia M,Dobchev D,Akhmedov NG,Hall CD,Bernier UR,Clark GG,Linthicum KJ

更新日期：2008-05-27 00:00:00

abstract：:The POU-IV or "Brn-3" class of POU-domain transcription factors is represented in Drosophila by I-POU and twin-of-I-POU, alternative splice products of the I-POU gene. I-POU has been previously reported to inhibit DNA binding by the POU-III class factor drifter/Cf1a via the formation of heterodimeric complexes. Here w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Turner EE

更新日期：1996-12-24 00:00:00

abstract：:Cryogenic fluorescent light microscopy of flash-frozen cells stands out by artifact-free fixation and very little photobleaching of the fluorophores used. To attain the highest level of resolution, aberration-free immersion objectives with accurately matched immersion media are required, but both do not exist for imag...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Faoro R,Bassu M,Mejia YX,Stephan T,Dudani N,Boeker C,Jakobs S,Burg TP

更新日期：2018-02-06 00:00:00

abstract：:Two mutations are described that alter the pattern of inheritance of chloroplast genes in Chlamydomonas. The mutant gene mat-1 linked to the mating type allele mt(-) greatly increases the frequency of exceptional zygotes, i.e., zygotes that transmit chloroplast genes from the mt(-) (male) parent. In some crosses, 80-9...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sager R,Ramanis Z

更新日期：1974-12-01 00:00:00

abstract：:The heterodimeric Elongin BC complex has been shown to interact in vitro and in cells with a conserved BC-box motif found in an increasing number of proteins including RNA polymerase II elongation factor Elongin A, suppressor of cytokine signaling (SOCS)-box proteins, and the von Hippel-Lindau tumor suppressor protein...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brower CS,Sato S,Tomomori-Sato C,Kamura T,Pause A,Stearman R,Klausner RD,Malik S,Lane WS,Sorokina I,Roeder RG,Conaway JW,Conaway RC

更新日期：2002-08-06 00:00:00

abstract：:Voltage-gated Na channels in several classes of neurons, including cells of the cerebellum, are subject to an open-channel block and unblock by an endogenous protein. The Na(V)beta4 (Scn4b) subunit is a candidate blocking protein because a free peptide from its cytoplasmic tail, the beta4 peptide, can block open Na ch...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bant JS,Raman IM

更新日期：2010-07-06 00:00:00

abstract：:Infected cell protein 0 (ICP0) is a 775-residue multifunctional herpes simplex virus protein associated with numerous functions related to transactivation of gene expression and repression of host defenses to infection. We report that an uncharted domain of ICP0 located between residues 245 and 510 contains multiple S...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liang Y,Kurakin A,Roizman B

更新日期：2005-04-19 00:00:00

abstract：:The amino-terminal sequences of two polypeptides with nonsuppressible insulin-like and cell-growth-promoting activities (NSILA I and II), isolated from human serum, were determined. Of the first 31 residues, 22 are identical in NSILA I and II. Moreover, a striking structural similarity was found between NSILA and insu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rinderknecht E,Humbel RE

更新日期：1976-12-01 00:00:00

abstract：:Metabolic engineering seeks to reprogram microbial cells to efficiently and sustainably produce value-added compounds. Since chemical production can be at odds with the cell's natural objectives, strategies have been developed to balance conflicting goals. For example, dynamic regulation modulates gene expression to f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dinh CV,Prather KLJ

更新日期：2019-12-17 00:00:00

abstract：:The sex pheromone biosynthetic pathways of lepidopterans require the concerted actions of multiple gene products. A number of pheromone gland (PG)-specific genes have been cloned in recent years and, whereas in vitro characterizations have indicated functions consistent with roles in pheromone production, there have b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ohnishi A,Hull JJ,Matsumoto S

更新日期：2006-03-21 00:00:00

abstract：:The traditional morphologically grounded placement of South American guinea pig-like rodents (Caviomorpha) within one of the two rodent suborders, Hystricognathi, has been disputed by recent analysis of protein and nucleic acid sequence data. The Caviomorpha and possibly all Hystricognathi would be considered a separa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Martignetti JA,Brosius J

更新日期：1993-10-15 00:00:00

abstract：:The basolateral membranes of Aplysia californica foregut absorptive cells contain both Cl(-)-stimulated ATPase and ATP-dependent Cl- transport activities, and each was inhibited by orthovanadate. Both of these orthovanadate-sensitive activities were reconstituted into proteoliposomes. The reaction sequence kinetics we...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gerencser GA,Zelezna B

更新日期：1993-09-01 00:00:00

abstract：:Changes in protein function can lead to changes in the selection acting on specific residues. This can often be detected as evolutionary rate changes at the sites in question. A maximum-likelihood method for detecting evolutionary rate shifts at specific protein positions is presented. The method determines significan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Knudsen B,Miyamoto MM

更新日期：2001-12-04 00:00:00

abstract：:Ovulation induces cyclic rupture and regenerative repair of the ovarian coelomic epithelium. This process of repeated disruption and repair accompanied by complex remodeling typifies a somatic stem/progenitor cell-mediated process. Using BrdU incorporation and doxycycline inducible histone2B-green fluorescent protein ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Szotek PP,Chang HL,Brennand K,Fujino A,Pieretti-Vanmarcke R,Lo Celso C,Dombkowski D,Preffer F,Cohen KS,Teixeira J,Donahoe PK

更新日期：2008-08-26 00:00:00

abstract：:To achieve the mission of personalized medicine, centering on delivering the right drug to the right patient at the right dose, therapeutic drug monitoring solutions are necessary. In that regard, wearable biosensing technologies, capable of tracking drug pharmacokinetics in noninvasively retrievable biofluids (e.g., ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin S,Yu W,Wang B,Zhao Y,En K,Zhu J,Cheng X,Zhou C,Lin H,Wang Z,Hojaiji H,Yeung C,Milla C,Davis RW,Emaminejad S

更新日期：2020-08-11 00:00:00

abstract：:Highly purified RNA polymerase II was found to be able to weakly recognize the initiator (Inr) present in the adenovirus IVa2 and major late promoters. The association of RNA polymerase II with the Inr was enhanced by the general transcription factors. The Inr was capable of directing the formation of a DNA-protein co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carcamo J,Buckbinder L,Reinberg D

更新日期：1991-09-15 00:00:00

abstract：:Resting- or baseline-state low-frequency (0.01-0.2 Hz) brain activity is observed in fMRI, EEG, and local field potential recordings. These fluctuations were found to be correlated across brain regions and are thought to reflect neuronal activity fluctuations between functionally connected areas of the brain. However,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krishnan GP,González OC,Bazhenov M

更新日期：2018-06-26 00:00:00

abstract：:O-GlcNAc glycosylation (or O-GlcNAcylation) is a dynamic, inducible posttranslational modification found on proteins associated with neurodegenerative diseases such as α-synuclein, amyloid precursor protein, and tau. Deletion of the O-GlcNAc transferase (ogt) gene responsible for the modification causes early postnata...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang AC,Jensen EH,Rexach JE,Vinters HV,Hsieh-Wilson LC

更新日期：2016-12-27 00:00:00

abstract：:The HB-19 pseudopeptide 5[Kpsi(CH(2)N)PR]-TASP, psi(CH(2)N) for reduced peptide bond, is a specific inhibitor of HIV infection in different CD4(+) cell lines and in primary T-lymphocytes and macrophages. It blocks virus-particle attachment to permissive cells by binding and forming a stable complex with nucleolin expr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krust B,Vienet R,Cardona A,Rougeot C,Jacotot E,Callebaut C,Guichard G,Briand JP,Grognet JM,Hovanessian AG,Edelman L

更新日期：2001-11-20 00:00:00

abstract：:Innervation of skeletal muscle by motor neurons occurs through the neuromuscular junction, a cholinergic synapse essential for normal muscle growth and function. Defects in nerve-muscle signaling cause a variety of neuromuscular disorders with features of ataxia, paralysis, skeletal muscle wasting, and degeneration. H...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anderson DM,Cannavino J,Li H,Anderson KM,Nelson BR,McAnally J,Bezprozvannaya S,Liu Y,Lin W,Liu N,Bassel-Duby R,Olson EN

更新日期：2016-08-02 00:00:00

abstract：:The brain continuously influences and perceives the physiological condition of the body. Related cortical representations have been proposed to shape emotional experience and guide behavior. Although previous studies have identified brain regions recruited during autonomic processing, neurological lesion studies have ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guo CC,Sturm VE,Zhou J,Gennatas ED,Trujillo AJ,Hua AY,Crawford R,Stables L,Kramer JH,Rankin K,Levenson RW,Rosen HJ,Miller BL,Seeley WW

更新日期：2016-04-26 00:00:00

abstract：:Coiled-coil motifs are ubiquitous mediators of specific protein-protein interactions through the formation of interlocking hydrophobic seams between alpha-helical chains. Residues that form these seams occur at the first (a) and fourth (d) positions of a characteristic 7-aa repeat and are primarily aliphatic. The pote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu J,Yong W,Deng Y,Kallenbach NR,Lu M

更新日期：2004-11-16 00:00:00

abstract：:Acetylcholine is the main neurotransmitter at the mammalian neuromuscular junction (NMJ) where nicotinic acetylcholine receptors mediate the signaling between nerve terminals and muscle fibers. We show that under glutamatergic transmission, rat NMJ switches from cholinergic type synapse to glutamatergic synapse. Conne...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brunelli G,Spano P,Barlati S,Guarneri B,Barbon A,Bresciani R,Pizzi M

更新日期：2005-06-14 00:00:00

abstract：:Hierarchical cellular structures are ubiquitous in nature because of their low-density, high-specific properties, and multifunctionality. Inspired by these systems, we created lightweight ceramic architectures composed of closed-cell porous struts patterned in the form of hexagonal and triangular honeycombs by direct ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Muth JT,Dixon PG,Woish L,Gibson LJ,Lewis JA

更新日期：2017-02-21 00:00:00

abstract：:Coagulation is an innate defense mechanism intended to limit blood loss and trap invading pathogens during infection. However, Staphylococcus aureus has the ability to hijack the coagulation cascade and generate clots via secretion of coagulases. Although many S. aureus have this characteristic, some do not. The popul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Trivedi U,Madsen JS,Everett J,Fell C,Russel J,Haaber J,Crosby HA,Horswill AR,Burmølle M,Rumbaugh KP,Sørensen SJ

更新日期：2018-12-11 00:00:00

abstract：:An integral mitochondrial membrane protein (p32) of yeast has previously been molecularly cloned and sequenced and suggested to function as a mitochondrial import receptor. However, this protein has also been proposed to function as phosphate translocator [Guérin, B., Bukusoglu, C., Rakotomanana, F. & Wohlrab, H. (199...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murakami H,Blobel G,Pain D

更新日期：1993-04-15 00:00:00

abstract：:The rugged folding-energy landscapes of RNAs often display many competing minima. How do RNAs discriminate among competing conformations in their search for the native state? By using optical tweezers, we show that the folding-energy landscape can be manipulated to control the fate of an RNA: individual RNA molecules ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li PT,Bustamante C,Tinoco I Jr

更新日期：2007-04-24 00:00:00