Identifying the subproteome of kinetically stable proteins via diagonal 2D SDS/PAGE.

abstract：:Marine Archaea are important players among microbial plankton and significantly contribute to biogeochemical cycles, but details regarding their community structure and long-term seasonal activity and dynamics remain largely unexplored. In this study, we monitored the interannual archaeal community composition of abun...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hugoni M,Taib N,Debroas D,Domaizon I,Jouan Dufournel I,Bronner G,Salter I,Agogué H,Mary I,Galand PE

更新日期：2013-04-09 00:00:00

abstract：:Lithium is the most commonly used drug for the treatment of manic depressive illness. The precise mechanisms underlying its clinical efficacy remain unknown. We found that long-term exposure to lithium chloride dramatically protects cultured rat cerebellar, cerebral cortical, and hippocampal neurons against glutamate-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nonaka S,Hough CJ,Chuang DM

更新日期：1998-03-03 00:00:00

abstract：:A defect in the yeast GEF1 gene, a CLC chloride channel homolog leads to an iron requirement and cation sensitivity. The iron requirement is due to a failure to load Cu2+ onto a component of the iron uptake system, Fet3. This process, which requires both Gef1 and the Menkes disease Cu2+-ATPase yeast homolog Ccc2, occu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gaxiola RA,Yuan DS,Klausner RD,Fink GR

更新日期：1998-03-31 00:00:00

abstract：:Here, the ATP-binding, ATP hydrolysis, mispair-binding, sliding clamp formation, and Mlh1-Pms1 complex interaction properties of dominant mutant Msh2-Msh6 complexes have been characterized. The results demonstrate two mechanisms for dominance. In one, seen with the Msh6-S1036P and Msh6-G1067D mutant complexes, the mut...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hess MT,Mendillo ML,Mazur DJ,Kolodner RD

更新日期：2006-01-17 00:00:00

abstract：:IDIOTYPY HAS BEEN STUDIED IN ANTIBODIES AGAINST A PROTEIN ANTIGEN: hen ovalbumin. Various fractions of antisera to hen ovalbumin have been compared from the standpoint of the idiotypic specificities found on the immunoglobulins that they contain. Idiotypic specificities were found to be common to (a) antibodies that w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oudin J,Cazenave PA

更新日期：1971-10-01 00:00:00

abstract：:Spatial organization of the binding sites for the priming substrate, the template DNA, and the transcription inhibitor rifampicin (Rif) in Escherichia coli RNA polymerase (EC 2.7.7.6) was probed with chimeric compounds in which Rif is covalently attached to a ribonucleotide. The compounds bind to RNA polymerase in bif...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mustaev A,Zaychikov E,Severinov K,Kashlev M,Polyakov A,Nikiforov V,Goldfarb A

更新日期：1994-12-06 00:00:00

abstract：:The formation of several acyl groups and an amide group of Taxol is catalyzed by regioselective CoA thioester-dependent acyltransferases. Several full-length acyltransferase sequences, obtained from a cDNA library constructed from mRNA isolated from Taxus cuspidata cells induced for Taxol production with methyl jasmon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walker K,Long R,Croteau R

更新日期：2002-07-09 00:00:00

abstract：:Fish have somehow colonized isolated water bodies all over the world without human assistance. It has long been speculated that these colonization events are assisted by waterbirds, transporting fish eggs attached to their feet and feathers, yet empirical support for this is lacking. Recently, it was suggested that en...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lovas-Kiss Á,Vincze O,Löki V,Pallér-Kapusi F,Halasi-Kovács B,Kovács G,Green AJ,Lukács BA

更新日期：2020-07-07 00:00:00

abstract：:Plasmids containing the replication origin of the Escherichia coli chromosome (oriC plasmids) are unstable in certain recA strains of E. coli. However, they can be maintained more stably in other recA strains. This stable maintenance has allowed us to study the incompatibility properties of oriC plasmids. We have foun...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamaguchi K,Yamaguchi M,Tomizawa J

更新日期：1982-09-01 00:00:00

abstract：:We introduce the concept of hybridization chain reaction (HCR), in which stable DNA monomers assemble only upon exposure to a target DNA fragment. In the simplest version of this process, two stable species of DNA hairpins coexist in solution until the introduction of initiator strands triggers a cascade of hybridizat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dirks RM,Pierce NA

更新日期：2004-10-26 00:00:00

abstract：:This paper shows that the most predictable components of internal variability in coupled atmosphere-ocean models are remarkably similar to the most predictable components of climate models without interactive ocean dynamics (i.e., models whose ocean is represented by a 50-m-deep slab ocean mixed layer with no interact...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Srivastava A,DelSole T

更新日期：2017-02-28 00:00:00

abstract：:Immediately after focal retinal lesions, receptive fields (RFs) in primary visual cortex expand considerably, even when the retinal damage is limited to the photoreceptor layer. The time course of these changes suggests that mere lack of stimulation in the vicinity of the RF accompanied by stimulation in the surroundi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pettet MW,Gilbert CD

更新日期：1992-09-01 00:00:00

abstract：:Progress in ecosystem service science has been rapid, and there is now a healthy appetite among key public and private sector decision makers for this science. However, changing policy and management is a long-term project, one that raises a number of specific practical challenges. One impediment to broad adoption of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Polasky S,Tallis H,Reyers B

更新日期：2015-06-16 00:00:00

abstract：:Erythropoietin, the major hormone controlling red-cell production, is regulated in part through oxygen-dependent changes in the rate of transcription of its gene. Using transient transfection in HepG2 cells, we have defined a DNA sequence, located 120 base pairs 3' to the poly(A)-addition site of the mouse erythropoie...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pugh CW,Tan CC,Jones RW,Ratcliffe PJ

更新日期：1991-12-01 00:00:00

abstract：:There is great interest in understanding the mechanisms of expression underlying long-term potentiation (LTP). They are agreed to involve an increase in synaptic efficacy, which is described by three multiplicative parameters: p, the probability of neurotransmitter release; n, the number of active release sites; and q...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schulz PE

更新日期：1997-05-27 00:00:00

abstract：:The type specimen for Australopithecus africanus (Taung) includes a natural endocast that reproduces most of the external morphology of the right cerebral hemisphere and a fragment of fossilized face that articulates with the endocast. Despite the fact that Taung died between 3 and 4 y of age, the endocast reproduces ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Falk D,Zollikofer CP,Morimoto N,Ponce de León MS

更新日期：2012-05-29 00:00:00

abstract：:Conjugation of drugs with antibodies to surface endothelial antigens is a potential strategy for drug delivery to endothelium. We studied antibodies to platelet-endothelial adhesion molecule 1 (PECAM-1, a stably expressed endothelial antigen) as carriers for vascular immunotargeting. Although 125I-labeled anti-PECAM b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Muzykantov VR,Christofidou-Solomidou M,Balyasnikova I,Harshaw DW,Schultz L,Fisher AB,Albelda SM

更新日期：1999-03-02 00:00:00

abstract：:Group II intron RNAs self-splice in vitro but only at high salt and/or Mg2+ concentrations and have been thought to require proteins to stabilize their active structure for efficient splicing in vivo. Here, we show that a DEAD-box protein, CYT-19, can by itself promote the splicing and reverse splicing of the yeast aI...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mohr S,Matsuura M,Perlman PS,Lambowitz AM

更新日期：2006-03-07 00:00:00

abstract：:Expression of the Arabidopsis TRANSPARENT TESTA 2 (TT2) MYB family transcription factor leads to massive accumulation of proanthocyanidins (PAs) in hairy roots of Medicago truncatula. Microarray analysis showed that TT2 induces genes for flavonoid/PA biosynthesis, transcription factors, and a large number of genes of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pang Y,Peel GJ,Sharma SB,Tang Y,Dixon RA

更新日期：2008-09-16 00:00:00

abstract：:Antibodies to electrolectin, a lectin endogenous to embryonic skeletal muscle, have been used to study the distribution of electrolectin during myogenesis in L6 cells and rat primary muscle cultures. Antibody binding is highest to mononucleated cells and is low to myotubes in both systems. Binding is much lower to fib...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Podleski TR,Greenberg I

更新日期：1980-02-01 00:00:00

abstract：:Proteins need to be tightly regulated as they control biological processes in most normal cellular functions. The precise mechanisms of regulation are rarely completely understood but can involve binding of endogenous ligands and/or partner proteins at specific locations on a protein that can modulate function. Often,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ludlow RF,Verdonk ML,Saini HK,Tickle IJ,Jhoti H

更新日期：2015-12-29 00:00:00

abstract：:Costly interactions between species that arise as a by-product of ancestral similarities in communication signals are expected to persist only under specific evolutionary circumstances. Territorial aggression between species, for instance, is widely assumed to persist only when extrinsic barriers prevent niche diverge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Drury JP,Cowen MC,Grether GF

更新日期：2020-06-09 00:00:00

abstract：:Y chromosomes control essential male functions in many species, including sex determination and fertility. However, because of obstacles posed by repeat-rich heterochromatin, knowledge of Y chromosome sequences is limited to a handful of model organisms, constraining our understanding of Y biology across the tree of l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hall AB,Papathanos PA,Sharma A,Cheng C,Akbari OS,Assour L,Bergman NH,Cagnetti A,Crisanti A,Dottorini T,Fiorentini E,Galizi R,Hnath J,Jiang X,Koren S,Nolan T,Radune D,Sharakhova MV,Steele A,Timoshevskiy VA,Windbich

更新日期：2016-04-12 00:00:00

abstract：:Molecular diversity of ion channel structure and function underlies variability in electrical signaling in nerve, muscle, and nonexcitable cells. Regulation by variable auxiliary subunits is a major mechanism to generate tissue- or cell-specific diversity of ion channel function. Mammalian large-conductance, voltage- ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yan J,Aldrich RW

更新日期：2012-05-15 00:00:00

abstract：:The reversible phosphorylation of tyrosine residues is an important mechanism for modulating biological processes such as cellular signaling, differentiation, and growth, and if deregulated, can result in various types of cancer. Therefore, an understanding of these dynamic cellular processes at the molecular level re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Salomon AR,Ficarro SB,Brill LM,Brinker A,Phung QT,Ericson C,Sauer K,Brock A,Horn DM,Schultz PG,Peters EC

更新日期：2003-01-21 00:00:00

abstract：:When treated with heat-killed bacterial cells, mosquito cells in culture respond by up-regulating several proteins. Among these is a 66-kDa protein (p66) that is secreted from cells derived from both Aedes aegypti and Aedes albopictus. p66 was degraded by proteolysis and gave a virtually identical pattern of peptide p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yoshiga T,Hernandez VP,Fallon AM,Law JH

更新日期：1997-11-11 00:00:00

abstract：:The transcription factor zinc-finger protein Miz1 represses TNF-α-induced JNK activation and the repression is relieved upon TNF-α stimulation. However, the underlying mechanism is incompletely understood. Here we report that Miz1 interferes with the ubiquitin conjugating enzyme (E2) Ubc13 for binding to the RING doma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu J,Yan J,Jiang S,Wen J,Chen L,Zhao Y,Lin A

更新日期：2012-01-03 00:00:00

abstract：:We propose a motility mechanism that may result in the displacement of objects within the cell. The mechanism, which we call polymer-guided diffusion, involves a microscopic cycle of polymer association and dissociation from a lateral binding site. Reassociation occurs at the polymer subunit adjacent to that which has...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Garel JR,Job D,Margolis RL

更新日期：1987-06-01 00:00:00

abstract：:Integrin alphanubeta3 is found on a subset of tumor blood vessels where it is associated with angiogenesis and malignant tumor growth. We designed an alphanubeta3-targeted nanoparticle (NP) encapsulating the cytotoxic drug doxorubicin (Dox) for targeted drug delivery to the alphanubeta3-expressing tumor vasculature. W...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murphy EA,Majeti BK,Barnes LA,Makale M,Weis SM,Lutu-Fuga K,Wrasidlo W,Cheresh DA

更新日期：2008-07-08 00:00:00

abstract：:The 5-hydroxytryptamine-3 (5-HT3) receptor mediates the fast excitatory neurotransmission of serotonin and is known to mediate the nausea/emesis induced by radio/chemotherapy and anesthetics. A polymorphism encoding the variation Y129S in the 5-HT3B subunit exists in high frequency in the general population and has be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krzywkowski K,Davies PA,Feinberg-Zadek PL,Bräuner-Osborne H,Jensen AA

更新日期：2008-01-15 00:00:00