Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry.

abstract：:The synthesis of a photosensitive blocking group for the carboxyl function of neurotransmitters, in this case glycine, is reported. The compound, 2-methoxy-5-nitrophenyl glycine ester (caged glycine), is photolyzed by a laser pulse at 308 or 337 nm within 3 microseconds and with a product quantum yield of 0.2. The com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ramesh D,Wieboldt R,Niu L,Carpenter BK,Hess GP

更新日期：1993-12-01 00:00:00

abstract：:Complex II inhibitors 3-nitropropionic acid (3NP) and malonate cause striatal damage reminiscent of Huntington's disease and have been shown to involve oxidative stress in their pathogenesis. Because nuclear factor erythroid 2-related factor 2 (Nrf2)-dependent transcriptional activation by means of the antioxidant res...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Calkins MJ,Jakel RJ,Johnson DA,Chan K,Kan YW,Johnson JA

更新日期：2005-01-04 00:00:00

abstract：:mRNA coding for mouse major transplantation antigens of the d haplotype was partially purified, copied into double-stranded cDNA, and cloned in Escherichia coli. Clones were selected by their ability to hybridize specifically with mRNA coding for H-2K, D, or L antigens. One of these clones, pH-2d-1, carries a 1200-bas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kvist S,Bregegere F,Rask L,Cami B,Garoff H,Daniel F,Wiman K,Larhammar D,Abastado JP,Gachelin G,Peterson PA,Dobberstein B,Kourilsky P

更新日期：1981-05-01 00:00:00

abstract：:Regulation of the turnover of triglycerides in adipose tissue requires the continuous provision of 3-glycerophosphate, which may be supplied by the metabolism of glucose or by glyceroneogenesis, the de novo synthesis of 3-glycerophosphate from sources other than hexoses or glycerol. The importance of glyceroneogenesis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Olswang Y,Cohen H,Papo O,Cassuto H,Croniger CM,Hakimi P,Tilghman SM,Hanson RW,Reshef L

更新日期：2002-01-22 00:00:00

abstract：:Agents that act as anion channel blockers (ACBs) and do not permeate cells appear to inhibit exocytosis in platelets, parathyroid cells, and neutrophils. Based in large part on these observations, anion influx through plasma membrane channels has been considered a factor controlling cellular secretion, but there have ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vostal JG,Reid DM,Jones CE,Shulman NR

更新日期：1989-08-01 00:00:00

abstract：:Activation-induced cytidine deaminase (AID) is essential to class-switch recombination (CSR) and somatic hypermutation (SHM) in both V region SHM and S region SHM (s-SHM). Uracil DNA glycosylase (UNG), a member of the base excision repair (BER) complex, is required for CSR. Strikingly, however, UNG deficiency causes a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yousif AS,Stanlie A,Mondal S,Honjo T,Begum NA

更新日期：2014-03-18 00:00:00

abstract：:Proinflammatory macrophages are key mediators in several pathologies; thus, controlling their activation is necessary. The endocannabinoid system is implicated in various inflammatory processes. Here we show that in macrophages, the newly characterized enzyme α/β-hydrolase domain 6 (ABHD6) controls 2-arachidonoylglyce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alhouayek M,Masquelier J,Cani PD,Lambert DM,Muccioli GG

更新日期：2013-10-22 00:00:00

abstract：:We report substantially improved performance of high-power quantum cascade lasers (QCLs) by using epitaxial-side-down mounting that provides superior heat dissipation properties. We used aluminum nitride as the heatsink material and gold-tin eutectic solder. We have obtained continuous wave power output of 450 mW at 2...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsekoun A,Go R,Pushkarsky M,Razeghi M,Patel CK

更新日期：2006-03-28 00:00:00

abstract：:Hydrothermal vent ecosystems support diverse life forms, many of which rely on symbiotic associations to perform functions integral to survival in these extreme physicochemical environments. Epsilonproteobacteria, found free-living and in intimate associations with vent invertebrates, are the predominant vent-associat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grzymski JJ,Murray AE,Campbell BJ,Kaplarevic M,Gao GR,Lee C,Daniel R,Ghadiri A,Feldman RA,Cary SC

更新日期：2008-11-11 00:00:00

abstract：:Structural differences between dynein and kinesin suggest a unique molecular mechanism of dynein motility. Measuring the mechanical properties of a single molecule of dynein is crucial for revealing the mechanisms underlying its movement. We measured the step size and force produced by single molecules of active cytop...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Toba S,Watanabe TM,Yamaguchi-Okimoto L,Toyoshima YY,Higuchi H

更新日期：2006-04-11 00:00:00

abstract：:Ultraviolet (UV)-stimulated nonconservative DNA synthesis has been observed in E. coli rendered permeable to nucleoside triphosphates by exposure to toluene. This synthesis is detected in toluenized cells in which the background of UV-independent DNA synthesis is reduced by use of dnaB mutants temperature-sensitive fo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Masker WE,Hanawalt PC

更新日期：1973-01-01 00:00:00

abstract：:Understanding the causes and consequences of wildfires in forests of the western United States requires integrated information about fire, climate changes, and human activity on multiple temporal scales. We use sedimentary charcoal accumulation rates to construct long-term variations in fire during the past 3,000 y in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marlon JR,Bartlein PJ,Gavin DG,Long CJ,Anderson RS,Briles CE,Brown KJ,Colombaroli D,Hallett DJ,Power MJ,Scharf EA,Walsh MK

更新日期：2012-02-28 00:00:00

abstract：:The liver plays a key role in the regulation of circulating levels of low density lipoproteins (LDL) because it is both the site for the production of and the major organ for the degradation of this class of lipoproteins. In this study, the effects of feeding polyunsaturated or saturated triacylglycerols on receptor-d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spady DK,Dietschy JM

更新日期：1985-07-01 00:00:00

abstract：:We identified cell surface markers associated with repression of p16(INK4a)/cyclin-dependent kinase inhibitor 2A(CDKN2A), a critical determinant in the acquisition of a plastic state. These cell surface markers allowed direct isolation of rare cells from healthy human breast tissue that exhibit extensive lineage plast...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roy S,Gascard P,Dumont N,Zhao J,Pan D,Petrie S,Margeta M,Tlsty TD

更新日期：2013-03-19 00:00:00

abstract：:Small noncoding microRNAs (miRNAs) can contribute to cancer development and progression and are differentially expressed in normal tissues and cancers. From a large-scale miRnome analysis on 540 samples including lung, breast, stomach, prostate, colon, and pancreatic tumors, we identified a solid cancer miRNA signatur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Volinia S,Calin GA,Liu CG,Ambs S,Cimmino A,Petrocca F,Visone R,Iorio M,Roldo C,Ferracin M,Prueitt RL,Yanaihara N,Lanza G,Scarpa A,Vecchione A,Negrini M,Harris CC,Croce CM

更新日期：2006-02-14 00:00:00

abstract：:Cholera and enterotoxigenic Escherichia coli (ETEC) are among the most common causes of acute infantile gastroenteritis globally. We previously developed a rice-based vaccine that expressed cholera toxin B subunit (MucoRice-CTB) and had the advantages of being cold chain-free and providing protection against cholera t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tokuhara D,Yuki Y,Nochi T,Kodama T,Mejima M,Kurokawa S,Takahashi Y,Nanno M,Nakanishi U,Takaiwa F,Honda T,Kiyono H

更新日期：2010-05-11 00:00:00

abstract：:Numerous studies have shown that high olive oil intake reduces blood pressure (BP). These positive effects of olive oil have frequently been ascribed to its minor components, such as alpha-tocopherol, polyphenols, and other phenolic compounds that are not present in other oils. However, in this study we demonstrate th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Terés S,Barceló-Coblijn G,Benet M,Alvarez R,Bressani R,Halver JE,Escribá PV

更新日期：2008-09-16 00:00:00

abstract：:Interleukin 1 (IL-1) and tumor necrosis factor alpha are thought to contribute to the inflammatory response associated with autoimmune diseases. Transforming growth factor beta 1 (TGF-beta 1) counteracts many effects of these cytokines and has various immunosuppressive properties. In the present study, it is shown tha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kuruvilla AP,Shah R,Hochwald GM,Liggitt HD,Palladino MA,Thorbecke GJ

更新日期：1991-04-01 00:00:00

abstract：:Microtubule-targeting agents (MTAs) are widely used for treatment of cancer and other diseases, and a detailed understanding of the mechanism of their action is important for the development of improved microtubule-directed therapies. Although there is a large body of data on the interactions of different MTAs with pu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mohan R,Katrukha EA,Doodhi H,Smal I,Meijering E,Kapitein LC,Steinmetz MO,Akhmanova A

更新日期：2013-05-28 00:00:00

abstract：:The leukotrienes C4, D4, and E4, previously referred to as slow reacting substance of anaphylaxis, elicited long-lasting contractions of bronchi isolated from two birch pollen-sensitive asthmatics. The leukotrienes were 1,000 times more potent on a molar basis than was histamine or prostaglandin F2 alpha. Moreover, al...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dahlén SE,Hansson G,Hedqvist P,Björck T,Granström E,Dahlén B

更新日期：1983-03-01 00:00:00

abstract：:Multiple sclerosis is a chronic disease of the central nervous system (CNS) characterized by inflammation, demyelination, and axonal loss. The immunopathogenesis of demyelination in multiple sclerosis involves an autoantibody response to myelin oligodendrocyte glycoprotein (MOG), a type I transmembrane protein located...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Breithaupt C,Schubart A,Zander H,Skerra A,Huber R,Linington C,Jacob U

更新日期：2003-08-05 00:00:00

abstract：:Mucopolysaccharidosis type VII is a lysosomal storage disorder resulting from inherited deficiency of beta-glucuronidase (GUS). Mucopolysaccharidosis type VII is characterized by glycosaminoglycan storage in most tissues, including brain. In these disorders, enzyme delivery across the blood-brain barrier (BBB) is the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Urayama A,Grubb JH,Sly WS,Banks WA

更新日期：2004-08-24 00:00:00

abstract：:von Willebrand factor (VWF), a protein present in our circulatory system, is necessary to stop bleeding under high shear-stress conditions as found in small blood vessels. The results presented here help unravel how an increase in hydrodynamic shear stress activates VWF's adhesion potential, leading to the counterintu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schneider SW,Nuschele S,Wixforth A,Gorzelanny C,Alexander-Katz A,Netz RR,Schneider MF

更新日期：2007-05-08 00:00:00

abstract：:Compartmentalization of biomolecules within lipid membranes is a fundamental requirement of living systems and an essential feature of many pharmaceutical therapies. However, applications of membrane-enclosed solutions of proteins, DNA, and other biologically active compounds have been limited by the difficulty of for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stachowiak JC,Richmond DL,Li TH,Liu AP,Parekh SH,Fletcher DA

更新日期：2008-03-25 00:00:00

abstract：:Epigenetics regulation plays a critical role in determining cell identity by controlling the accessibility of lineage-specific regulatory regions. In muscle stem cells, epigenetic mechanisms of how chromatin accessibility is modulated during cell fate determination are not fully understood. Here, we identified a long ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dong A,Preusch CB,So WK,Lin K,Luan S,Yi R,Wong JW,Wu Z,Cheung TH

更新日期：2020-12-22 00:00:00

abstract：:Influenza virus neuraminidase (NA; EC 3.2.1.18) possesses a signal-anchor hydrophobic domain at the amino terminus. To characterize the nature of this signal-anchor domain we have introduced single amino acid changes in this domain by oligonucleotide-directed mutagenesis. Three mutant NA proteins that were synthesized...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sivasubramanian N,Nayak DP

更新日期：1987-01-01 00:00:00

abstract：:Site-directed mutagenesis of selected residues of mammalian protein phosphatase 1 (PP-1) has been carried out to further define the mechanism of catalysis, activation by divalent cations, and inhibition by toxins and inhibitory proteins. Mutation of active site residues predicted to bind metals (N124D and H248N) resul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang HB,Horiuchi A,Goldberg J,Greengard P,Nairn AC

更新日期：1997-04-15 00:00:00

abstract：:Peroxisome proliferators (PP) induce a highly predictable pleiotropic response in rat and mouse liver that is characterized by hepatomegaly, increase in peroxisome number in hepatocytes, and induction of certain peroxisomal enzymes. The PP-binding protein (PPbP) was purified from rat liver cytosol by a two-step proced...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lalwani ND,Alvares K,Reddy MK,Reddy MN,Parikh I,Reddy JK

更新日期：1987-08-01 00:00:00

abstract：:Rhinoviruses (RVs) are the major causes of common colds in humans. They have a nonenveloped, icosahedral capsid surrounding a positive-strand RNA genome. Here we report that the antigen-binding (Fab) fragment of a neutralizing antibody (C5) can trigger genome release from RV-B14 to form emptied particles and neutraliz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dong Y,Liu Y,Jiang W,Smith TJ,Xu Z,Rossmann MG

更新日期：2017-07-25 00:00:00

abstract：:Loss-of-function mutations in the genes associated with primary microcephaly (MCPH) reduce human brain size by about two-thirds, without producing gross abnormalities in brain organization or physiology and leaving other organs largely unaffected [Woods CG, et al. (2005) Am J Hum Genet 76:717-728]. There is also evide...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rimol LM,Agartz I,Djurovic S,Brown AA,Roddey JC,Kähler AK,Mattingsdal M,Athanasiu L,Joyner AH,Schork NJ,Halgren E,Sundet K,Melle I,Dale AM,Andreassen OA,Alzheimer's Disease Neuroimaging Initiative.

更新日期：2010-01-05 00:00:00