Podocin and MEC-2 bind cholesterol to regulate the activity of associated ion channels.

abstract：:Antibody raised against the human erythrocyte glucose transporter identified a recombinant lambda gt11 bacteriophage in a cDNA library prepared from immunoselected polysomal RNA from adult rat brain. The cDNA predicts a 492-amino acid protein that demonstrates 97.6% identity to the human hepatoma hexose carrier. The t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Birnbaum MJ,Haspel HC,Rosen OM

更新日期：1986-08-01 00:00:00

abstract：:The kidney has an extraordinary ability to maintain stable fractional solute and fluid reabsorption over a wide range of glomerular filtration rates (GFRs). Internalization of filtered low molecular weight proteins, vitamins, hormones, and other small molecules is mediated by the proximal tubule (PT) multiligand recep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raghavan V,Rbaibi Y,Pastor-Soler NM,Carattino MD,Weisz OA

更新日期：2014-06-10 00:00:00

abstract：:During the last several decades corals have been in decline and at least one-third of all coral species are now threatened with extinction. Coral disease has been a major contributor to this threat, but little is known about the responsible pathogens. To date most research has focused on bacterial and fungal diseases;...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vega Thurber RL,Barott KL,Hall D,Liu H,Rodriguez-Mueller B,Desnues C,Edwards RA,Haynes M,Angly FE,Wegley L,Rohwer FL

更新日期：2008-11-25 00:00:00

abstract：:Given their relatively small area, mangroves and their organic sediments are of disproportionate importance to global carbon sequestration and carbon storage. Peat deposition and preservation allows some mangroves to accrete vertically and keep pace with sea-level rise by growing on their own root remains. In this stu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ezcurra P,Ezcurra E,Garcillán PP,Costa MT,Aburto-Oropeza O

更新日期：2016-04-19 00:00:00

abstract：:In vivo, G protein-coupled receptors (GPCR) for neurotransmitters undergo complex intracellular trafficking that contribute to regulate their abundance at the cell surface. Here, we report a previously undescribed alteration in the subcellular localization of D1 dopamine receptor (D1R) that occurs in vivo in striatal ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dumartin B,Jaber M,Gonon F,Caron MG,Giros B,Bloch B

更新日期：2000-02-15 00:00:00

abstract：:Trypanosomatid RNA editing is a unique process and essential for these organisms. It therefore represents a drug target for a group of protozoa that includes the causative agents for African sleeping sickness and other devastating tropical and subtropical diseases. Here, we present drug-like inhibitors of a key enzyme...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Amaro RE,Schnaufer A,Interthal H,Hol W,Stuart KD,McCammon JA

更新日期：2008-11-11 00:00:00

abstract：:Proper initiation of transcription by RNA polymerase II requires the TATA-consensus-binding transcription factor TFIID. A cDNA clone encoding the Drosophila TFIID protein has been isolated and characterized. The deduced amino acid sequence reveals an open reading frame of 353 residues. The carboxyl-terminal 180 amino ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Muhich ML,Iida CT,Horikoshi M,Roeder RG,Parker CS

更新日期：1990-12-01 00:00:00

abstract：:Climate change is increasing fire activity in the western United States, which has the potential to accelerate climate-induced shifts in vegetation communities. Wildfire can catalyze vegetation change by killing adult trees that could otherwise persist in climate conditions no longer suitable for seedling establishmen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Davis KT,Dobrowski SZ,Higuera PE,Holden ZA,Veblen TT,Rother MT,Parks SA,Sala A,Maneta MP

更新日期：2019-03-26 00:00:00

abstract：:We have grown crystals of a soluble recombinant form of human CD4, a transmembrane glycoprotein found predominantly on the surface of helper T cells. Crystals composed of the entire extracellular portion of CD4 exhibit extensive polymorphism. Of the five crystal types that have been grown, the best diffracts to Bragg ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kwong PD,Ryu SE,Hendrickson WA,Axel R,Sweet RM,Folena-Wasserman G,Hensley P,Sweet RW

更新日期：1990-08-01 00:00:00

abstract：:N6-Cyclohexyl[3H]adenosine ([3H]CHA) and 1,3-diethyl-8-[3H]phenylxanthine ([3H]DPX) to bind to adenosine receptors in brain membranes. The agonist [3H]CHA has high affinity in both bovine and guinea pig brain (Kd, 0.7 nM and 6 nM, respectively). [3H]CHA binding kinetics are slow (dissociation t1/2;60 min); binding is ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bruns RF,Daly JW,Snyder SH

更新日期：1980-09-01 00:00:00

abstract：:Fine finger and hand movements in humans, monkeys, and rats are under the direct control of the corticospinal tract (CST). CST lesions lead to severe, long-term deficits of precision movements. We transected completely both CSTs in adult rats and treated the animals for 2 weeks with an antibody that neutralized the ce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raineteau O,Fouad K,Noth P,Thallmair M,Schwab ME

更新日期：2001-06-05 00:00:00

abstract：:After more than two decades of study, many fundamental questions remain unanswered about the dynamics of glass-forming materials confined to thin films. Experiments and simulations indicate that free interfaces enhance dynamics over length scales larger than molecular sizes, and this effect strengthens at lower temper...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ivancic RJS,Riggleman RA

更新日期：2020-10-13 00:00:00

abstract：:Neutron diffraction maps have been used to assign the rotor conformations of the hydroxyl hydrogens in trypsin. Knowledge of these conformations is used to assess the relative importance of steric and electrostatic effects in conferring the H-bonding geometries of these groups. A general finding was that most hydroxyl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kossiakoff AA,Shpungin J,Sintchak MD

更新日期：1990-06-01 00:00:00

abstract：:The cell surface proteome, the surfaceome, is the interface for engaging the extracellular space in normal and cancer cells. Here we apply quantitative proteomics of N-linked glycoproteins to reveal how a collection of some 700 surface proteins is dramatically remodeled in an isogenic breast epithelial cell line stabl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leung KK,Wilson GM,Kirkemo LL,Riley NM,Coon JJ,Wells JA

更新日期：2020-04-07 00:00:00

abstract：:Caveolin, a 21- to 24-kDa integral membrane protein, is a principal component of caveolae membranes. Caveolin interacts directly with heterotrimeric guanine nucleotide binding proteins (G proteins) and can functionally regulate their activity. Here, an approximately 20-kDa caveolin-related protein, caveolin-2, was ide...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scherer PE,Okamoto T,Chun M,Nishimoto I,Lodish HF,Lisanti MP

更新日期：1996-01-09 00:00:00

abstract：:Nitrogen limitation imposes a major transition in the lifestyle of nondiazotrophic cyanobacteria that is controlled by a complex interplay of regulatory factors involving the pervasive signal processor PII Immediately upon nitrogen limitation, newly fixed carbon is redirected toward glycogen synthesis. How the metabol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Orthwein T,Scholl J,Spät P,Lucius S,Koch M,Macek B,Hagemann M,Forchhammer K

更新日期：2021-02-09 00:00:00

abstract：:Activation of naïve cluster of differentiation (CD)8(+) cytotoxic T lymphocytes (CTLs) is a tightly regulated process, and specific dendritic cell (DC) subsets are typically required to activate naive CTLs. Potential pathways for antigen presentation leading to CD8(+) T-cell priming include direct presentation, cross-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li L,Kim S,Herndon JM,Goedegebuure P,Belt BA,Satpathy AT,Fleming TP,Hansen TH,Murphy KM,Gillanders WE

更新日期：2012-07-31 00:00:00

abstract：:Evidence is given for the existence of an electron transport pathway to oxygen in the thylakoid membranes of chloroplasts (chlororespiration). Plastoquinone is shown to be a redox carrier common to both photosynthetic and chlororespiratory pathways. It is shown that, in dark-adapted chloroplasts, an electrochemical gr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bennoun P

更新日期：1982-07-01 00:00:00

abstract：:A coenzyme B12-dependent ribonucleotide reductase was purified from the archaebacterium Thermoplasma acidophila and partially sequenced. Using probes derived from the sequence, the corresponding gene was cloned, completely sequenced, and expressed in Escherichia coli. The deduced amino acid sequence shows that the cat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tauer A,Benner SA

更新日期：1997-01-07 00:00:00

abstract：:The absorption spectrum of an activesite specific chromophoric acyl enzyme, sturgeon beta-(2-furyl)-acryloyl-glyceraldehyde-3-phosphate dehydrogenase, is reported. This acyl enzyme undergoes all of the catalyzed reactions characteristic of the intermediate of the physiological acyl enzyme, 3-phospho-D-glyceroyl-glycer...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Malhotra OP,Bernhard SA

更新日期：1973-07-01 00:00:00

abstract：:Accurate modification of the 3 billion-base-pair human genome requires tools with exceptional sequence specificity. Here, we describe a general strategy for the design of enzymes that target a single site within the genome. We generated chimeric zinc finger recombinases with cooperative DNA-binding and catalytic speci...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gordley RM,Gersbach CA,Barbas CF 3rd

更新日期：2009-03-31 00:00:00

abstract：:We describe a strategy to "revive" putatively pathogenic T cells from frozen specimens of human inflammatory target organs. To distinguish pathogenic from irrelevant bystander T cells, we focused on cells that were (i) clonally expanded and (ii) in direct morphological contact with a target cell. Using CDR3 spectratyp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seitz S,Schneider CK,Malotka J,Nong X,Engel AG,Wekerle H,Hohlfeld R,Dornmair K

更新日期：2006-08-08 00:00:00

abstract：:The three-dimensional structures of avian H5 and swine H9 influenza hemagglutinins (HAs) from viruses closely related to those that caused outbreaks of human disease in Hong Kong in 1997 and 1999 were determined bound to avian and human cell receptor analogs. Emerging influenza pandemics have been accompanied by the e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ha Y,Stevens DJ,Skehel JJ,Wiley DC

更新日期：2001-09-25 00:00:00

abstract：:Influenza hemagglutinin (HA) is the primary target of the humoral response during infection/vaccination. Current influenza vaccines typically fail to elicit/boost broadly neutralizing antibodies (bnAbs), thereby limiting their efficacy. Although several bnAbs bind to the conserved stem domain of HA, focusing the immun...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mallajosyula VV,Citron M,Ferrara F,Lu X,Callahan C,Heidecker GJ,Sarma SP,Flynn JA,Temperton NJ,Liang X,Varadarajan R

更新日期：2014-06-24 00:00:00

abstract：:Cytokines such as interleukin 4 (IL-4) play a key role in the regulation of immune responses, but little is known about how their multiple activities are regulated in vivo. In this report, we demonstrated that an IL-4-binding protein (IL-4BP) is constitutively present in the biological fluids of mice (serum, ascites, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fernandez-Botran R,Vitetta ES

更新日期：1990-06-01 00:00:00

abstract：:Despite substantial size variations, proportions of the developing body plan are maintained with a remarkable precision. Little is known about the mechanisms that ensure this adaptation (scaling) of pattern with size. Most models of patterning by morphogen gradients do not support scaling. In contrast, we show that sc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ben-Zvi D,Barkai N

更新日期：2010-04-13 00:00:00

abstract：:Mutational activation of the Ras oncogene products (H-Ras, K-Ras, and N-Ras) is frequently observed in human cancers, making them promising anticancer drug targets. Nonetheless, no effective strategy has been available for the development of Ras inhibitors, partly owing to the absence of well-defined surface pockets s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shima F,Yoshikawa Y,Ye M,Araki M,Matsumoto S,Liao J,Hu L,Sugimoto T,Ijiri Y,Takeda A,Nishiyama Y,Sato C,Muraoka S,Tamura A,Osoda T,Tsuda K,Miyakawa T,Fukunishi H,Shimada J,Kumasaka T,Yamamoto M,Kataoka T

更新日期：2013-05-14 00:00:00

abstract：:The wide diversity of dendritic trees is one of the most striking features of neural circuits. Here we develop a general quantitative theory relating the total length of dendritic wiring to the number of branch points and synapses. We show that optimal wiring predicts a 2/3 power law between these measures. We demonst...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cuntz H,Mathy A,Häusser M

更新日期：2012-07-03 00:00:00

abstract：:An unprecedented, spontaneous, and complete cleavage of the triple bond of N2 in the thermal reaction of 15N2 with Ta214N+ was observed experimentally by Fourier transform ion cyclotron resonance mass spectrometry; mechanistic aspects of the degenerate ligand exchange were addressed by high-level quantum chemical calc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Geng C,Li J,Weiske T,Schwarz H

更新日期：2019-10-22 00:00:00

abstract：:In all organisms, mild heat pretreatments induce tolerance to high temperatures. In the yeast Saccharomyces cerevisiae, such pretreatments strongly induce heat-shock protein (Hsp) 104, and hsp104 mutations greatly reduce high-temperature survival, indicating Hsp1O4 plays a critical role in induced thermotolerance. Sur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lindquist S,Kim G

更新日期：1996-05-28 00:00:00