X-ray structures of H5 avian and H9 swine influenza virus hemagglutinins bound to avian and human receptor analogs.

Abstract:

:The three-dimensional structures of avian H5 and swine H9 influenza hemagglutinins (HAs) from viruses closely related to those that caused outbreaks of human disease in Hong Kong in 1997 and 1999 were determined bound to avian and human cell receptor analogs. Emerging influenza pandemics have been accompanied by the evolution of receptor-binding specificity from the preference of avian viruses for sialic acid receptors in alpha2,3 linkage to the preference of human viruses for alpha2,6 linkages. The four new structures show that HA binding sites specific for human receptors appear to be wider than those preferring avian receptors and how avian and human receptors are distinguished by atomic contacts at the glycosidic linkage. alpha2,3-Linked sialosides bind the avian HA in a trans conformation to form an alpha2,3 linkage-specific motif, made by the glycosidic oxygen and 4-OH of the penultimate galactose, that is complementary to the hydrogen-bonding capacity of Gln-226, an avian-specific residue. alpha2,6-Linked sialosides bind in a cis conformation, exposing the glycosidic oxygen to solution and nonpolar atoms of the receptor to Leu-226, a human-specific residue. The new structures are compared with previously reported crystal structures of HA/sialoside complexes of the H3 subtype that caused the 1968 Hong Kong Influenza virus pandemic and analyzed in relation to HA sequences of all 15 subtypes and to receptor affinity data to make clearer how receptor-binding sites of HAs from avian viruses evolve as the virus adapts to humans.

authors

Ha Y,Stevens DJ,Skehel JJ,Wiley DC

doi

10.1073/pnas.201401198

keywords:

subject

Has Abstract

pub_date

2001-09-25 00:00:00

pages

11181-6

issue

20

eissn

0027-8424

issn

1091-6490

pii

201401198

journal_volume

98

pub_type

杂志文章
  • Predicting protein function from structure: unique structural features of proteases.

    abstract::We have noted consistent structural similarities among unrelated proteases. In comparison with other proteins of similar size, proteases have smaller than average surface areas, smaller radii of gyration, and higher C(alpha) densities. These findings imply that proteases are, as a group, more tightly packed than other...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.070548997

    authors: Stawiski EW,Baucom AE,Lohr SC,Gregoret LM

    更新日期:2000-04-11 00:00:00

  • Analysis of a Picard modular group.

    abstract::Our main goal is to analyze the geometric and spectral properties of the Picard modular group with Gaussian integer entries acting on the two-dimensional complex hyperbolic space. ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0603075103

    authors: Francsics G,Lax PD

    更新日期:2006-07-25 00:00:00

  • Physical limits to spatial resolution of optical recording: clarifying the spatial structure of cortical hypercolumns.

    abstract::Neurons in macaque primary visual cortex are spatially arranged by their global topographic position and in at least three overlapping local modular systems: ocular dominance columns, orientation pinwheels, and cytochrome oxidase (CO) blobs. Individual neurons in the blobs are not tuned to orientation, and populations...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0500291102

    authors: Polimeni JR,Granquist-Fraser D,Wood RJ,Schwartz EL

    更新日期:2005-03-15 00:00:00

  • Large-scale sequencing of the CD33-related Siglec gene cluster in five mammalian species reveals rapid evolution by multiple mechanisms.

    abstract::Siglecs are a recently discovered family of animal lectins that belong to the Ig superfamily and recognize sialic acids (Sias). CD33-related Siglecs (CD33rSiglecs) are a subgroup with as-yet-unknown functions, characterized by sequence homology, expression on innate immune cells, conserved cytosolic tyrosine-based sig...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0404833101

    authors: Angata T,Margulies EH,Green ED,Varki A

    更新日期:2004-09-07 00:00:00

  • Searching for missing heritability: designing rare variant association studies.

    abstract::Genetic studies have revealed thousands of loci predisposing to hundreds of human diseases and traits, revealing important biological pathways and defining novel therapeutic hypotheses. However, the genes discovered to date typically explain less than half of the apparent heritability. Because efforts have largely foc...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1322563111

    authors: Zuk O,Schaffner SF,Samocha K,Do R,Hechter E,Kathiresan S,Daly MJ,Neale BM,Sunyaev SR,Lander ES

    更新日期:2014-01-28 00:00:00

  • Interleukin-6 is an essential, corticotropin-releasing hormone-independent stimulator of the adrenal axis during immune system activation.

    abstract::Glucocorticoids play a critical role in control of the cytokine response after immune challenge. Conversely, cytokines modulate glucocorticoid production by the hypothalamic-pituitary-adrenal axis. To define the potency and mechanism of interleukin-6 (IL-6) for augmentation of adrenal function, we exploited mice defic...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.97.16.9317

    authors: Bethin KE,Vogt SK,Muglia LJ

    更新日期:2000-08-01 00:00:00

  • Isolation of lysosomal alpha-mannosidase mutants of Chinese hamster ovary cells.

    abstract::A method is presented for detection of lysosomal hydrolase activity in situ in colonies of Chinese hamster ovary cells. This method was used to screen for mutants deficient in lysosomal alpha-mannosidase. Mutagenized cells were replicated onto filter papers [Esko, J. D. & Raetz, C. R. H. (1978) Proc. Natl. Acad. Sci. ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.76.4.1911

    authors: Robbins AR

    更新日期:1979-04-01 00:00:00

  • MR1 antigen presentation to mucosal-associated invariant T cells was highly conserved in evolution.

    abstract::Several nonclassical major histocompatibilty antigens (class Ib molecules) have emerged as key players in the early immune response to pathogens or stress. Class Ib molecules activate subsets of T cells that mount effector responses before the adaptive immune system, and thus are called innate T cells. MR1 is a novel ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0903196106

    authors: Huang S,Martin E,Kim S,Yu L,Soudais C,Fremont DH,Lantz O,Hansen TH

    更新日期:2009-05-19 00:00:00

  • Differential requirements of MHC and of DCs for endogenous proliferation of different T-cell subsets in vivo.

    abstract::T cells transferred into severe lymphopenic hosts undergo rapid proliferation known as "endogenous proliferation" that are distinct from conventional homeostatic proliferation. Unlike homeostatic proliferation, cytokines, such as IL-7 are dispensable, yet TCR:MHC interaction is essential for this process to occur. How...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0909954106

    authors: Do JS,Min B

    更新日期:2009-12-01 00:00:00

  • High-pressure superconducting phase diagram of 6Li: isotope effects in dense lithium.

    abstract::We measured the superconducting transition temperature of (6)Li between 16 and 26 GPa, and report the lightest system to exhibit superconductivity to date. The superconducting phase diagram of (6)Li is compared with that of (7)Li through simultaneous measurement in a diamond anvil cell (DAC). Below 21 GPa, Li exhibits...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1412638112

    authors: Schaeffer AM,Temple SR,Bishop JK,Deemyad S

    更新日期:2015-01-06 00:00:00

  • Hepatitis C virus RNA in southern African blacks with hepatocellular carcinoma.

    abstract::We used a sensitive and specific cDNA polymerase chain reaction assay to detect hepatitis C virus (HCV) RNA in serum samples from 128 unselected southern African Blacks with hepatocellular carcinoma (HCC) and found HCV RNA in 26 (20.3%) of these patients. Antibodies to HCV (anti-HCV) were detected in 59 patients (46.1...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.90.5.1848

    authors: Bukh J,Miller RH,Kew MC,Purcell RH

    更新日期:1993-03-01 00:00:00

  • Crystal structure of the MurG:UDP-GlcNAc complex reveals common structural principles of a superfamily of glycosyltransferases.

    abstract::MurG is an essential glycosyltransferase that forms the glycosidic linkage between N-acetyl muramyl pentapeptide and N-acetyl glucosamine in the biosynthesis of the bacterial cell wall. This enzyme is a member of a major superfamily of NDP-glycosyltransferases for which no x-ray structures containing intact substrates...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0235749100

    authors: Hu Y,Chen L,Ha S,Gross B,Falcone B,Walker D,Mokhtarzadeh M,Walker S

    更新日期:2003-02-04 00:00:00

  • The gluconate operon gnt of Bacillus subtilis encodes its own transcriptional negative regulator.

    abstract::The gluconate (gnt) operon of Bacillus subtilis consists of four gnt genes; the second and third genes code for gluconate kinase (gluconokinase, EC 2.7.1.12) and gluconate permease, respectively. A fragment carrying the promoter of this operon (gnt promoter) and the first gene (gntR) was subcloned into a promoter prob...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.84.13.4524

    authors: Fujita Y,Fujita T

    更新日期:1987-07-01 00:00:00

  • Nucleic acid transport through carbon nanotube membranes.

    abstract::We study the electrophoretic transport of single-stranded RNA molecules through 1.5-nm-wide pores of carbon nanotube membranes by molecular dynamics simulations. From approximately 170 individual RNA translocation events analyzed at full atomic resolution of solvent, membrane, and RNA, we identify key factors in membr...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0402699101

    authors: Yeh IC,Hummer G

    更新日期:2004-08-17 00:00:00

  • Temperature-sensitive tumorigenicity of cells transformed by a mutant of Moloney sarcoma virus.

    abstract::Normal rat kidney cells were nonproductively infected either with CP27, a mutant of Moloney sarcoma virus that is temperature-sensitive for maintenance of transformation, or with the parental wild-type virus. The nonproducer cells were inoculated into the tails of athymic nude mice that were subsequently incubated at ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.77.3.1501

    authors: Klarlund JK,Forchhammer J

    更新日期:1980-03-01 00:00:00

  • Biosynthesis of vitamin B12: structure of precorrin-6x octamethyl ester.

    abstract::13C-labeled precorrin-6x is biosynthesized by cell-free protein preparations from Pseudomonas denitrificans in separate experiments using delta-amino[5-13C]levulinic acid and the corresponding delta-amino[4-13C]- and delta-amino[3-13C]levulinic acid-labeled forms in conjunction with S-[methyl-13C]adenosylmethionine fo...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.87.22.8800

    authors: Thibaut D,Blanche F,Debussche L,Leeper FJ,Battersby AR

    更新日期:1990-11-01 00:00:00

  • Human growth hormone promotes engraftment of murine or human T cells in severe combined immunodeficient mice.

    abstract::Growth hormone (GH) has previously been implicated in T-cell development, and here we test its efficacy in promoting T-lymphocyte engraftment in mice with severe combined immune deficiency (SCID). SCID mice receiving syngeneic thymocytes and treated with recombinant human GH (rhGH) had significantly better T-cell engr...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.89.10.4481

    authors: Murphy WJ,Durum SK,Longo DL

    更新日期:1992-05-15 00:00:00

  • Polycation-siRNA nanoparticles can disassemble at the kidney glomerular basement membrane.

    abstract::Despite being engineered to avoid renal clearance, many cationic polymer (polycation)-based siRNA nanoparticles that are used for systemic delivery are rapidly eliminated from the circulation. Here, we show that a component of the renal filtration barrier--the glomerular basement membrane (GBM)--can disassemble cation...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1200718109

    authors: Zuckerman JE,Choi CH,Han H,Davis ME

    更新日期:2012-02-21 00:00:00

  • Release of N2,3-ethenoguanine from chloroacetaldehyde-treated DNA by Escherichia coli 3-methyladenine DNA glycosylase II.

    abstract::The human carcinogen vinyl chloride is metabolized in the liver to reactive intermediates which form N2,3-ethenoguanine in DNA. N2,3-Ethenoguanine is known to cause G----A transitions during DNA replication in Escherichia coli, and its formation may be a carcinogenic event in higher organisms. To investigate the repai...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.89.19.9331

    authors: Matijasevic Z,Sekiguchi M,Ludlum DB

    更新日期:1992-10-01 00:00:00

  • Microinjected progesterone reinitiates meiotic maturation of Xenopus laevis oocytes.

    abstract::Microinjection of progesterone dissolved in paraffin oil induces the reinitiation of meiotic maturation in the Xenopus oocyte; 50% maturation is obtained when 50 nl of a 50 microM solution is microinjected into the oocyte. The kinetics of the response to microinjected progesterone are similar to the kinetics of respon...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.79.18.5552

    authors: Tso J,Thibier C,Mulner O,Ozon R

    更新日期:1982-09-01 00:00:00

  • Sperm-specific protein kinase A catalytic subunit Calpha2 orchestrates cAMP signaling for male fertility.

    abstract::An unusual cAMP signaling system mediates many of the events that prepare spermatozoa to meet the egg. Its components include the atypical, bicarbonate-stimulated, sperm adenylyl cyclase and a cAMP-dependent protein kinase (PKA) with the unique catalytic subunit termed Calpha(2) or C(s). We generated mice that lack Ca...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0405580101

    authors: Nolan MA,Babcock DF,Wennemuth G,Brown W,Burton KA,McKnight GS

    更新日期:2004-09-14 00:00:00

  • Platelet-derived growth factor beta receptor regulates interstitial fluid homeostasis through phosphatidylinositol-3' kinase signaling.

    abstract::Platelet-derived growth factor (PDGF) isoforms lead to mitogenic, survival, and chemotactic responses in a variety of mesenchymal cell types during development and in the adult. We have studied the importance of phosphatidylinositol-3' kinase (PI3K) signaling in these responses by mutating the PI3K-binding sites in th...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.96.20.11410

    authors: Heuchel R,Berg A,Tallquist M,Ahlén K,Reed RK,Rubin K,Claesson-Welsh L,Heldin CH,Soriano P

    更新日期:1999-09-28 00:00:00

  • Brain white matter structure and COMT gene are linked to second-language learning in adults.

    abstract::Adult human brains retain the capacity to undergo tissue reorganization during second-language learning. Brain-imaging studies show a relationship between neuroanatomical properties and learning for adults exposed to a second language. However, the role of genetic factors in this relationship has not been investigated...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1606602113

    authors: Mamiya PC,Richards TL,Coe BP,Eichler EE,Kuhl PK

    更新日期:2016-06-28 00:00:00

  • Detection of secondary binding sites in proteins using fragment screening.

    abstract::Proteins need to be tightly regulated as they control biological processes in most normal cellular functions. The precise mechanisms of regulation are rarely completely understood but can involve binding of endogenous ligands and/or partner proteins at specific locations on a protein that can modulate function. Often,...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1518946112

    authors: Ludlow RF,Verdonk ML,Saini HK,Tickle IJ,Jhoti H

    更新日期:2015-12-29 00:00:00

  • Establishment of a cell line from lepidopteran wing imaginal discs: Induction of newly synthesized proteins by 20-hydroxyecdysone.

    abstract::A cell line has been developed from the imaginal wing discs of the lepidopteran insect Trichoplusia ni (Hübner). The cells grow in suspension as fluid-filled, multicellular vesicles. The cell line has been designated IAL-TND1. Two-dimensional electrophoresis (isoelectric focusing and sodium dodecyl sulfate/polyacrylam...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.79.8.2589

    authors: Lynn DE,Miller SG,Oberlander H

    更新日期:1982-04-01 00:00:00

  • F901318 represents a novel class of antifungal drug that inhibits dihydroorotate dehydrogenase.

    abstract::There is an important medical need for new antifungal agents with novel mechanisms of action to treat the increasing number of patients with life-threatening systemic fungal disease and to overcome the growing problem of resistance to current therapies. F901318, the leading representative of a novel class of drug, the...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1608304113

    authors: Oliver JD,Sibley GEM,Beckmann N,Dobb KS,Slater MJ,McEntee L,du Pré S,Livermore J,Bromley MJ,Wiederhold NP,Hope WW,Kennedy AJ,Law D,Birch M

    更新日期:2016-11-08 00:00:00

  • A functional R domain from cystic fibrosis transmembrane conductance regulator is predominantly unstructured in solution.

    abstract::Phosphorylation of the regulatory (R) domain initiates cystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channel activity. To discover how the function of this domain is determined by its structure, we produced an R domain protein (R8) that spanned residues 708-831 of CFTR. Phosphorylated, but not unpho...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.100588797

    authors: Ostedgaard LS,Baldursson O,Vermeer DW,Welsh MJ,Robertson AD

    更新日期:2000-05-09 00:00:00

  • Mitotic Golgi disassembly is required for bipolar spindle formation and mitotic progression.

    abstract::During mitosis, the mammalian Golgi vesiculates and, upon partitioning, reassembles in each daughter cell; however, it is not clear whether the disassembly process per se is important for partitioning or is merely an outcome of mitotic entry. Here, we show that Golgi vesiculation is required for progression to metapha...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1610844113

    authors: Guizzunti G,Seemann J

    更新日期:2016-10-25 00:00:00

  • Neurotensin induces IL-6 secretion in mouse preadipocytes and adipose tissues during 2,4,6,-trinitrobenzensulphonic acid-induced colitis.

    abstract::Mesenteric fat is known to undergo inflammatory changes after 2,4,6,-trinitrobenzensulphonic acid (TNBS)-induced colitis. Neurotensin (NT) and neurotensin receptor 1 (NTR1) have been shown to play a major role in the pathogenesis of intestinal inflammation. This led us to explore whether NT and NTR1 are expressed in t...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0903499106

    authors: Koon HW,Kim YS,Xu H,Kumar A,Zhao D,Karagiannides I,Dobner PR,Pothoulakis C

    更新日期:2009-05-26 00:00:00

  • TMEM59 potentiates Wnt signaling by promoting signalosome formation.

    abstract::Wnt/β-catenin signaling controls development and adult tissue homeostasis by regulating cell proliferation and cell fate decisions. Wnt binding to its receptors Frizzled (FZD) and low-density lipoprotein-related 6 (LRP6) at the cell surface initiates a signaling cascade that leads to the transcription of Wnt target ge...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1721321115

    authors: Gerlach JP,Jordens I,Tauriello DVF,van 't Land-Kuper I,Bugter JM,Noordstra I,van der Kooij J,Low TY,Pimentel-Muiños FX,Xanthakis D,Fenderico N,Rabouille C,Heck AJR,Egan DA,Maurice MM

    更新日期:2018-04-24 00:00:00