Abstract:
:Activation of naïve cluster of differentiation (CD)8(+) cytotoxic T lymphocytes (CTLs) is a tightly regulated process, and specific dendritic cell (DC) subsets are typically required to activate naive CTLs. Potential pathways for antigen presentation leading to CD8(+) T-cell priming include direct presentation, cross-presentation, and cross-dressing. To distinguish between these pathways, we designed single-chain trimer (SCT) peptide-MHC class I complexes that can be recognized as intact molecules but cannot deliver antigen to MHC through conventional antigen processing. We demonstrate that cross-dressing is a robust pathway of antigen presentation following vaccination, capable of efficiently activating both naïve and memory CD8(+) T cells and requires CD8α(+)/CD103(+) DCs. Significantly, immune responses induced exclusively by cross-dressing were as strong as those induced exclusively through cross-presentation. Thus, cross-dressing is an important pathway of antigen presentation, with important implications for the study of CD8(+) T-cell responses to viral infection, tumors, and vaccines.
journal_name
Proc Natl Acad Sci U S Aauthors
Li L,Kim S,Herndon JM,Goedegebuure P,Belt BA,Satpathy AT,Fleming TP,Hansen TH,Murphy KM,Gillanders WEdoi
10.1073/pnas.1203468109subject
Has Abstractpub_date
2012-07-31 00:00:00pages
12716-21issue
31eissn
0027-8424issn
1091-6490pii
1203468109journal_volume
109pub_type
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