Influenza hemagglutinin stem-fragment immunogen elicits broadly neutralizing antibodies and confers heterologous protection.

abstract：:The heterotrimeric complex between integrin-linked kinase (ILK), PINCH, and parvin is an essential signaling platform, serving as a convergence point for integrin and growth-factor signaling and regulating cell adhesion, spreading, and migration. We report a 1.6-A crystal structure of the ILK ankyrin repeat domain bou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chiswell BP,Zhang R,Murphy JW,Boggon TJ,Calderwood DA

更新日期：2008-12-30 00:00:00

abstract：:Hypomagnesemia affects insulin resistance and is a risk factor for diabetes mellitus type 2 (DM2) and gestational diabetes mellitus (GDM). Two single nucleotide polymorphisms (SNPs) in the epithelial magnesium channel TRPM6 (V(1393)I, K(1584)E) were predicted to confer susceptibility for DM2. Here, we show using patch...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nair AV,Hocher B,Verkaart S,van Zeeland F,Pfab T,Slowinski T,Chen YP,Schlingmann KP,Schaller A,Gallati S,Bindels RJ,Konrad M,Hoenderop JG

更新日期：2012-07-10 00:00:00

abstract：:The Brazilian Amazon is one of the most rapidly developing agricultural areas in the world and represents a potentially large future source of greenhouse gases from land clearing and subsequent agricultural management. In an integrated approach, we estimate the greenhouse gas dynamics of natural ecosystems and agricul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Galford GL,Melillo JM,Kicklighter DW,Cronin TW,Cerri CE,Mustard JF,Cerri CC

更新日期：2010-11-16 00:00:00

abstract：:Linear double-stranded molecules of the circularly permuted and terminally redundant DNA of Salmonella bacteriophage P22 have been converted to oligomeric products in the presence of polynucleotide ligase coded for by the coliphage T4. The reaction has been monitored by sucrose density-gradient centrifugation and elec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sgaramella V

更新日期：1972-11-01 00:00:00

abstract：:A principal goal of cancer nanomedicine is to deliver therapeutics effectively to cancer cells within solid tumors. However, there are a series of biological barriers that impede nanomedicine from reaching target cells. Here, we report a stimuli-responsive clustered nanoparticle to systematically overcome these multip...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li HJ,Du JZ,Du XJ,Xu CF,Sun CY,Wang HX,Cao ZT,Yang XZ,Zhu YH,Nie S,Wang J

更新日期：2016-04-12 00:00:00

abstract：:Relatively little is understood about the dynamics of global host-pathogen transcriptome changes that occur during bacterial infection of mucosal surfaces. To test the hypothesis that group A Streptococcus (GAS) infection of the oropharynx provokes a distinct host transcriptome response, we performed genome-wide trans...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shea PR,Virtaneva K,Kupko JJ 3rd,Porcella SF,Barry WT,Wright FA,Kobayashi SD,Carmody A,Ireland RM,Sturdevant DE,Ricklefs SM,Babar I,Johnson CA,Graham MR,Gardner DJ,Bailey JR,Parnell MJ,Deleo FR,Musser JM

更新日期：2010-03-09 00:00:00

abstract：:The refractoriness of acute promyelocytic leukemia (APL) with t(11;17)(q23;q21) to all-trans retinoic acid (ATRA)-based therapy concerns clinicians and intrigues basic researchers. By using a murine leukemic model carrying both promyelocytic leukemia zinc finger/retinoic acid receptor-α (PLZF/RARα) and RARα/PLZF fusio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jiao B,Ren ZH,Liu P,Chen LJ,Shi JY,Dong Y,Ablain J,Shi L,Gao L,Hu JP,Ren RB,de Thé H,Chen Z,Chen SJ

更新日期：2013-02-26 00:00:00

abstract：:To find the native conformation (fold), proteins sample a subspace that is typically hundreds of orders of magnitude smaller than their full conformational space. Whether such fast folding is intrinsic or the result of natural selection, and what is the longest foldable protein, are open questions. Here, we derive the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin MM,Zewail AH

更新日期：2012-06-19 00:00:00

abstract：:Burkitt's lymphomas, linked by previous studies with the DNA-containing Epstein-Barr virus, contain an RNA related in sequence to that of Rauscher leukemia virus. The present study establishes that the viral-related RNA found in Burkitt's tumors is a 70S component encapsulated with RNA-instructed DNA polymerase in a p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kufe D,Magrath IT,Ziegler JL,Spiegelman S

更新日期：1973-03-01 00:00:00

abstract：:Depression is a debilitating mental illness and is often comorbid with metabolic disorders such as type 2 diabetes. Adiponectin is an adipocyte-derived hormone with antidiabetic and insulin-sensitizing properties. Here we show that adiponectin levels in plasma are reduced in a chronic social-defeat stress model of dep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu J,Guo M,Zhang D,Cheng SY,Liu M,Ding J,Scherer PE,Liu F,Lu XY

更新日期：2012-07-24 00:00:00

abstract：:A microfluidic mixer is applied to study the kinetics of calmodulin conformational changes upon Ca2+ binding. The device facilitates rapid, uniform mixing by decoupling hydrodynamic focusing from diffusive mixing and accesses time scales of tens of microseconds. The mixer is used in conjunction with multiphoton micros...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park HY,Kim SA,Korlach J,Rhoades E,Kwok LW,Zipfel WR,Waxham MN,Webb WW,Pollack L

更新日期：2008-01-15 00:00:00

abstract：:Initiation of bovine papillomavirus (BPV) DNA synthesis in vivo and in vitro depends on the interaction of the viral initiator protein E1 with the replication origin (ori+ DNA). The viral E2 protein assists this interaction, resulting in a cooperative assembly of both proteins on the replication origin. Using gel mobi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lusky M,Hurwitz J,Seo YS

更新日期：1994-09-13 00:00:00

abstract：:In everyday listening, sound reaches our ears directly from a source as well as indirectly via reflections known as reverberation. Reverberation profoundly distorts the sound from a source, yet humans can both identify sound sources and distinguish environments from the resulting sound, via mechanisms that remain uncl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Traer J,McDermott JH

更新日期：2016-11-29 00:00:00

abstract：:Vibrio cholerae O1 exists as two major serotypes, Inaba and Ogawa, which are associated with the O antigen of the lipopolysaccharide and are capable of unequal reciprocal interconversion. The 20-kilobase rfb regions encoding O-antigen biosynthesis in strains 569B (Inaba) and O17 (Ogawa) have been cloned in Escherichia...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stroeher UH,Karageorgos LE,Morona R,Manning PA

更新日期：1992-04-01 00:00:00

abstract：:Mutations in the gene encoding the transcription factor autoimmune regulator (AIRE) are responsible for autoimmune polyendocrinopathy candidiasis ectodermal dystrophy syndrome. AIRE directs expression of tissue-restricted antigens in the thymic medulla and in lymph node stromal cells and thereby substantially contribu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pomié C,Vicente R,Vuddamalay Y,Lundgren BA,van der Hoek M,Enault G,Kagan J,Fazilleau N,Scott HS,Romagnoli P,van Meerwijk JP

更新日期：2011-07-26 00:00:00

abstract：:A recently determined set of 20 NMR-derived conformations of a 48-residue all-alpha-helical protein, (PDB ID code 2JVD), is validated here by comparing the observed (13)C(alpha) chemical shifts with those computed at the density functional level of theory. In addition, a recently introduced physics-based method, aimed...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vila JA,Aramini JM,Rossi P,Kuzin A,Su M,Seetharaman J,Xiao R,Tong L,Montelione GT,Scheraga HA

更新日期：2008-09-23 00:00:00

abstract：:The ability of materials to define the architecture and microenvironment experienced by cells provides new opportunities to direct the fate of human pluripotent stem cells (HPSCs) [Robinton DA, Daley GQ (2012) Nature 481(7381):295-305]. However, the conditions required for self-renewal vs. differentiation of HPSCs are...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dixon JE,Shah DA,Rogers C,Hall S,Weston N,Parmenter CD,McNally D,Denning C,Shakesheff KM

更新日期：2014-04-15 00:00:00

abstract：:The accuracy of the 3'-end processing by cleavage and polyadenylation is essential for mRNA biogenesis and transcription termination. In yeast, two poorly conserved neighboring elements upstream of cleavage sites are important for accuracy and efficiency of this process. These two RNA sequences are recognized by the R...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barnwal RP,Lee SD,Moore C,Varani G

更新日期：2012-12-26 00:00:00

abstract：:We investigated the operation of a posttranslational protein translocation pathway to determine whether ions are excluded from the translocase during protein transport. The membrane capacitance during protein translocation across chloroplast thylakoid membranes was monitored via electric-field-indicating carotenoid el...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Teter SA,Theg SM

更新日期：1998-02-17 00:00:00

abstract：:The rearrangement of immunoglobulin genes in B-lymphocyte precursors requires the expression of the recombination activating genes (Rag), which leads to the generation of a highly diverse B-cell repertoire. We can use the level of Rag-1 mRNA in the bone marrow as an index of its capacity to support the maturation of B...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ben-Yehuda A,Szabo P,Dyall R,Weksler ME

更新日期：1994-12-06 00:00:00

abstract：:The tissue-specific pattern of mRNA expression can indicate important clues about gene function. High-density oligonucleotide arrays offer the opportunity to examine patterns of gene expression on a genome scale. Toward this end, we have designed custom arrays that interrogate the expression of the vast majority of pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Su AI,Wiltshire T,Batalov S,Lapp H,Ching KA,Block D,Zhang J,Soden R,Hayakawa M,Kreiman G,Cooke MP,Walker JR,Hogenesch JB

更新日期：2004-04-20 00:00:00

abstract：:In the wake of the COVID-19 pandemic many countries implemented containment measures to reduce disease transmission. Studies using digital data sources show that the mobility of individuals was effectively reduced in multiple countries. However, it remains unclear whether these reductions caused deeper structural chan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schlosser F,Maier BF,Jack O,Hinrichs D,Zachariae A,Brockmann D

更新日期：2020-12-29 00:00:00

abstract：:Paleoanthropologists and vertebrate paleontologists have for decades debated the etiology of tooth wear and its implications for understanding the diets of human ancestors and other extinct mammals. The debate has recently taken a twist, calling into question the efficacy of dental microwear to reveal diet. Some argue...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xia J,Zheng J,Huang D,Tian ZR,Chen L,Zhou Z,Ungar PS,Qian L

更新日期：2015-08-25 00:00:00

abstract：:Recent climate change has increased arctic soil temperatures and thawed large areas of permafrost, allowing for microbial respiration of previously frozen C. Furthermore, soil destabilization from melting ice has caused an increase in thermokarst failures that expose buried C and release dissolved organic C (DOC) to s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cory RM,Crump BC,Dobkowski JA,Kling GW

更新日期：2013-02-26 00:00:00

abstract：:Surfactant replacement therapy (SRT) involves instillation of a liquid-surfactant mixture directly into the lung airway tree. It is widely successful for treating surfactant deficiency in premature neonates who develop neonatal respiratory distress syndrome (NRDS). However, when applied to adults with acute respirator...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Filoche M,Tai CF,Grotberg JB

更新日期：2015-07-28 00:00:00

abstract：:Intermediate filaments-cytoplasmic filaments with a diameter of 8-9 nm-have been described in a large variety of cell types. In this study, the subunit protein of the neurofilament and the presumptive subunit of the astroglial filament are compared by immunological and biochemical methods and are found to be very simi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yen SH,Dahl D,Schachner M,Shelanski ML

更新日期：1976-02-01 00:00:00

abstract：:The partial purification of mouse mammary gland stem cells (MaSCs) using combinatorial cell surface markers (Lin(-)CD24(+)CD29(h)CD49f(h)) has improved our understanding of their role in normal development and breast tumorigenesis. Despite the significant improvement in MaSC enrichment, there is presently no methodolo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： dos Santos CO,Rebbeck C,Rozhkova E,Valentine A,Samuels A,Kadiri LR,Osten P,Harris EY,Uren PJ,Smith AD,Hannon GJ

更新日期：2013-04-30 00:00:00

abstract：:The first step in the replication of the adenovirus genome is the covalent attachment of the 5'-terminal nucleotide, dCMP, to the virus-encoded terminal protein precursor (pTP). This reaction can be observed in vitro and has been previously shown to be dependent upon either viral DNA or linearized plasmid DNA containi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Challberg MD,Rawlins DR

更新日期：1984-01-01 00:00:00

abstract：:The binding of bovine TSH (thyroid stimulating hormone), LH (luteinizing hormone), and their subunits to the TSH receptor of beef thyroid membranes was compared to stimulation by these agents of adenylate cyclase [ATP pyrophosphate-lyase(cyelizing), EC 4.6.1.1] in the same membranes, glucose oxidation in dog thyroid s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wolff J,Winand RJ,Kohn LD

更新日期：1974-09-01 00:00:00

abstract：:As a second-order nonlinear optical process, sum-frequency generation is highly surface-specific and accordingly has been developed into a very powerful and versatile surface spectroscopic tool. It has found many unique applications in different disciplines and thus provided many exciting new research opportunities in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shen YR

更新日期：1996-10-29 00:00:00