Discovery of drug-like inhibitors of an essential RNA-editing ligase in Trypanosoma brucei.

abstract：:Previous studies have failed to detect an interaction between monomeric soluble CD4 (sCD4) and class II major histocompatibility complex (MHC) proteins, suggesting that oligomerization of CD4 on the cell surface may be required to form a stable class II MHC binding site. To test this possibility, we transfected the F4...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sakihama T,Smolyar A,Reinherz EL

更新日期：1995-07-03 00:00:00

abstract：:Cystic fibrosis (CF) is caused by mutations in the gene encoding the cystic fibrosis transmembrane conductance regulator (CFTR) anion channel. Airway disease is the major source of morbidity and mortality. Successful implementation of gene- and cell-based therapies for CF airway disease requires knowledge of relations...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shah VS,Ernst S,Tang XX,Karp PH,Parker CP,Ostedgaard LS,Welsh MJ

更新日期：2016-05-10 00:00:00

abstract：:To understand non-trivial biological functions, it is crucial to develop minimal synthetic models that capture their basic features. Here, we demonstrate a sequence-independent, reversible control of transcription and gene expression using a photosensitive nucleic acid binder (pNAB). By introducing a pNAB whose affini...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Estévez-Torres A,Crozatier C,Diguet A,Hara T,Saito H,Yoshikawa K,Baigl D

更新日期：2009-07-28 00:00:00

abstract：:It was reported that the rate of elongation of partially reconstituted RNA, if prepared without centrifugation, showed dependency upon the state of protein aggregation like that found when free RNA was used as the nucleic acid source [Butler, P. J. G. (1974) J. Mol. Biol. 82, 333-341]. Butler concluded from this resul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ohno T,Takahashi M,Okada Y

更新日期：1977-02-01 00:00:00

abstract：:Glucocorticoids play a critical role in control of the cytokine response after immune challenge. Conversely, cytokines modulate glucocorticoid production by the hypothalamic-pituitary-adrenal axis. To define the potency and mechanism of interleukin-6 (IL-6) for augmentation of adrenal function, we exploited mice defic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bethin KE,Vogt SK,Muglia LJ

更新日期：2000-08-01 00:00:00

abstract：:Genomic imprinting results in the differential expression of genes, depending on which allele is inherited from the mother and which from the father. The effects of such differential gene expression are reflected in phenotypic differences between the reciprocal heterozygotes (Aa vs. aA). Although many imprinted genes ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheverud JM,Hager R,Roseman C,Fawcett G,Wang B,Wolf JB

更新日期：2008-03-18 00:00:00

abstract：:Water dynamics in concentrated ionic solutions plays an important role in a number of material and energy conversion processes such as the charge transfer at the electrolyte-electrode interface in aqueous rechargeable ion batteries. One long-standing puzzle is that all electrolytes, regardless of their "structure-maki...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang Q,Wu T,Chen C,Mukamel S,Zhuang W

更新日期：2017-09-19 00:00:00

abstract：:Microtubule-associated protein 2 kinase (MAP kinase), which exists in several forms, is a protein serine/threonine kinase that participates in a growth factor-activated protein kinase cascade in which it activates a ribosomal protein S6 kinase (pp90rsk) while being regulated itself by a cytoplasmic factor (MAP kinase ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seger R,Ahn NG,Boulton TG,Yancopoulos GD,Panayotatos N,Radziejewska E,Ericsson L,Bratlien RL,Cobb MH,Krebs EG

更新日期：1991-07-15 00:00:00

abstract：:Aminoacyl-tRNA synthetases (amino acid-tRNA ligases, EC 6.1.1.-) catalyze the aminoacylation of specific amino acids onto their cognate tRNAs with extraordinary accuracy. Recent reports, however, indicate that this class of enzymes may play other roles in cellular metabolism. Several aminoacyl-tRNA synthetases are her...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rapaport E,Remy P,Kleinkauf H,Vater J,Zamecnik PC

更新日期：1987-11-01 00:00:00

abstract：:Polyaromatic hydrocarbons are ubiquitous environmental chemicals that are important mutagens and carcinogens. The purpose of this study was to determine whether genes within the major histocompatibility complex (MHC) influence their biological activities. Cell-mediated immunity to dimethylbenz(a)anthracene (DMBA) was ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Elmets CA,Athar M,Tubesing KA,Rothaupt D,Xu H,Mukhtar H

更新日期：1998-12-08 00:00:00

abstract：:A full-length cDNA clone encoding a purified augmenter of liver regeneration (ALR) factor prepared from the cytosol of weanling rat livers was isolated. The 1.2-kb cDNA included a 299-bp 5' untranslated region, a 375-bp coding region, and a 550-bp 3' untranslated region. It encoded a protein consisting of 125 amino ac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hagiya M,Francavilla A,Polimeno L,Ihara I,Sakai H,Seki T,Shimonishi M,Porter KA,Starzl TE

更新日期：1994-08-16 00:00:00

abstract：:The S region of the murine major histocompatibility complex contains two structurally related genes (21-OHase A and 21-OHase B) that encode 21-hydroxylase (21-OHase), an enzyme essential for the synthesis of adrenal steroids. Expression of these two genes has been analyzed by using oligonucleotide probes specific for ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Parker KL,Chaplin DD,Wong M,Seidman JG,Smith JA,Schimmer BP

更新日期：1985-12-01 00:00:00

abstract：:Chromatin insulators or boundaries are proposed to structure the chromatin fiber into functionally independent domains by promoting the formation of chromatin loops. These elements can block the communication between an enhancer and a gene when placed between them. Interestingly, it has been previously observed that t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Melnikova L,Juge F,Gruzdeva N,Mazur A,Cavalli G,Georgiev P

更新日期：2004-10-12 00:00:00

abstract：:During natural fertilization, mammalian spermatozoa must pass through the zona pellucida before reaching the plasma membrane of the oocyte. It is assumed that this step involves partial lysis of the zona by sperm acrosomal enzymes, but there has been no unequivocal evidence to support this view. Here we present eviden...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hirose M,Honda A,Fulka H,Tamura-Nakano M,Matoba S,Tomishima T,Mochida K,Hasegawa A,Nagashima K,Inoue K,Ohtsuka M,Baba T,Yanagimachi R,Ogura A

更新日期：2020-02-04 00:00:00

abstract：:A highly specific radioimmunoassay for one of the putative adrenomedullary [Met]enkephalin precursors, BAM-12P (Tyr-Gly-Gly-Phe-Met-Arg-Arg-Val-Gly-Arg-Pro-Glu-OH), has been developed. The BAM-12P antibodies recognize the COOH-terminal fragment of the peptide from Arg7 to Glu12 and do not crossreact with [Met5]- or [L...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baird A,Ling N,Böhlen P,Benoit R,Klepper R,Guillemin R

更新日期：1982-03-01 00:00:00

abstract：:We describe the use of a series of gradually expanded thymine nucleobase analogs in probing steric effects in DNA polymerase efficiency and fidelity. In these nonpolar compounds, the base size was increased incrementally over a 1.0-A range by use of variably sized atoms (H, F, Cl, Br, and I) to replace the oxygen mole...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim TW,Delaney JC,Essigmann JM,Kool ET

更新日期：2005-11-01 00:00:00

abstract：:Treatment with neuraminidase (NA) plus galactose oxidase (GalOxase) does not cause stimulation of human thymocytes. However, stimulation can be achieved by addition of exogenous interleukin 2 (IL-2). The IL-2-induced stimulation was inhibited with anti-Tac antibody, indicating that NA/GalOxase-oxidized cells can serve...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roffman E,Sredni B,Smolinsky A,Wilchek M

更新日期：1984-08-01 00:00:00

abstract：:Band 3, the major intrinsic protein of the human erythrocyte membrane, was specifically labeled with the covalent fluorescent probe eosin isothiocyanate. The lateral mobility of labeled band 3 in the plane of the membrane under various conditions of ionic strength and temperature was examined by using the fluorescence...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Golan DE,Veatch W

更新日期：1980-05-01 00:00:00

abstract：:The lack of genetic means has been a serious limitation in studying mosquito immunity. We generated Relish-mediated immune deficiency (RMID) by transforming Aedes aegypti with the Delta Rel transgene driven by the vitellogenin (Vg) promoter using the pBac[3xP3-EGFP, afm] vector. A stable transformed line had a single ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shin SW,Kokoza V,Lobkov I,Raikhel AS

更新日期：2003-03-04 00:00:00

abstract：:DNA replication intermediates in human melanoma cells have been investigated by using the drug aphidicolin, which inhibits DNA polymerase alpha. In untreated cells, Okazaki fragments and 10-kilobase (kb) DNA intermediates are formed. In aphidicolin-treated cells, the replication fork is stopped and there is no formati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lönn U,Lönn S

更新日期：1983-07-01 00:00:00

abstract：:Xenopus laevis tadpole tails contain fast muscle fibers oriented in chevrons and two pairs of slow muscle "cords" along the length of the tail. When tail resorption is inhibited by a number of different treatments, fast muscle but not the slow cord muscle still is lost, demonstrating that the fast tail muscle is a dir...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Das B,Schreiber AM,Huang H,Brown DD

更新日期：2002-09-17 00:00:00

abstract：:In this study, we describe the use of nuclease-resistant molecular beacons (MBs) for the real-time detection of coxsackievirus B6 replication in living Buffalo green monkey kidney (BGMK) cells via Tat peptide delivery. A nuclease-resistant MB containing 2'-O-methyl RNA bases with phosphorothioate internucleotide linka...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yeh HY,Yates MV,Mulchandani A,Chen W

更新日期：2008-11-11 00:00:00

abstract：:The exo isomer of aflatoxin B1 (AFB1) 8,9-epoxide appears to be the only product of AFB1 involved in reaction with DNA and reacts with the N7 atom of guanine via an SN2 reaction from an intercalated state. Although the epoxide hydrolyzes rapidly in H2O (0.6 s-1 at 25 degrees C), very high yields of DNA adduct result. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Johnson WW,Guengerich FP

更新日期：1997-06-10 00:00:00

abstract：:The "apoptotic ring" is characterized by the phosphorylation of histone H2AX at serine 139 (γ-H2AX) by DNA-dependent protein kinase (DNA-PK). The γ-H2AX apoptotic ring differs from the nuclear foci patterns observed in response to DNA-damaging agents. It contains phosphorylated DNA damage response proteins including a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Solier S,Kohn KW,Scroggins B,Xu W,Trepel J,Neckers L,Pommier Y

更新日期：2012-08-07 00:00:00

abstract：:Modern practice for training classification deepnets involves a terminal phase of training (TPT), which begins at the epoch where training error first vanishes. During TPT, the training error stays effectively zero, while training loss is pushed toward zero. Direct measurements of TPT, for three prototypical deepnet a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Papyan V,Han XY,Donoho DL

更新日期：2020-10-06 00:00:00

abstract：:Mechanisms that enabled primitive cell membranes to self-reproduce have been discussed based on the physicochemical properties of fatty acids; however, there must be a transition to modern cell membranes composed of phospholipids [Budin I, Szostak JW (2011) Proc Natl Acad Sci USA 108:5249-5254]. Thus, a growth-divisio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Terasawa H,Nishimura K,Suzuki H,Matsuura T,Yomo T

更新日期：2012-04-17 00:00:00

abstract：:It has been reported that an insulin 2 gene knockout, when bred onto nonobese diabetic (NOD) mice, accelerates diabetes. We produced insulin 1 gene knockout congenic NOD mice. In contrast to insulin 2, diabetes and insulitis were markedly reduced in insulin 1 knockout mice, with decreased and delayed diabetes in heter...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moriyama H,Abiru N,Paronen J,Sikora K,Liu E,Miao D,Devendra D,Beilke J,Gianani R,Gill RG,Eisenbarth GS

更新日期：2003-09-02 00:00:00

abstract：:A7r5 smooth muscle (aorta) cells have a spontaneous electrical activity. Application of vasopressin produces a hyperpolarization accompanied by an interruption of the spontaneous activity, which is followed by a depolarization associated with a recovery of the spiking activity. Vasopressin action is produced by an act...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Van Renterghem C,Romey G,Lazdunski M

更新日期：1988-12-01 00:00:00

abstract：:Stimulation of the region antero-ventral to the third cerebral ventricle (AV3V) by a cholinergic drug, carbachol, and lesions of the AV3V have been demonstrated in previous studies to either augment or decrease sodium excretion, respectively. Atrial natriuretic peptide (ANP) dramatically increases renal sodium excreti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baldissera S,Menani JW,dos Santos LF,Favaretto AL,Gutkowska J,Turrin MQ,McCann SM,Antunes-Rodrigues J

更新日期：1989-12-01 00:00:00

abstract：:We present the use of our recently described multiple-loci polymorphic DNA markers ("alumorphs") for linkage mapping of the human genome. By using the polymerase chain reaction (PCR) with an Alu-specific primer we could reveal, in a single experiment, up to 20 genomic polymorphisms seen as the presence or absence of a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zietkiewicz E,Labuda M,Sinnett D,Glorieux FH,Labuda D

更新日期：1992-09-15 00:00:00