当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Nitrendipine block of cardiac calcium channels: high-affinity binding to the inactivated state.

Nitrendipine block of cardiac calcium channels: high-affinity binding to the inactivated state.

Abstract：

:Block of Ca2+ currents by the dihydropyridine drug nitrendipine was studied in single canine ventricular cells by using the whole-cell variant of the patch clamp technique. When cells were held at depolarized membrane potentials at which Ca2+ currents were approximately equal to 70% inactivated, nitrendipine blocked Ca2+ currents very potently, with half-block by subnanomolar concentrations. The concentration dependence of block had the form expected for 1:1 binding, with an apparent dissociation constant (Kd) of 0.36 nM. In contrast, when cells were held at hyperpolarized potentials, nitrendipine blocked Ca2+ currents much less potently (Kd approximately equal to 700 nM). The results can be explained if nitrendipine binds very tightly to the inactivated state of the Ca2+ channel and only weakly to the normal resting state. The Kd estimated for binding to the inactivated state is very similar to the dissociation constants previously found for high-affinity [3H]nitrendipine binding to membrane fragments from heart, smooth muscle, brain, and other tissues; moreover, the concentration-dependent kinetics of binding to the inactivated state are similar to those reported for [3H]nitrendipine binding to membranes. These results make it seem very likely that the high-affinity [3H]nitrendipine binding site is an inactivated state of the Ca2+ channel.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Bean BP

doi

10.1073/pnas.81.20.6388

subject

Has Abstract

pub_date

1984-10-01 00:00:00

pages

6388-92

issue

20

eissn

0027-8424

issn

1091-6490

journal_volume

81

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Analysis of the mechanism, rate, and sites of proteolytic cleavage of human immunoglobulin D by high-pressure liquid chromatography.

    abstract：:The high susceptibility of human immunoglobulin D to proteolytic degradation affects its biological function, metabolism, and immunoassay. High-pressure liquid chromatography was used to investigate the mechanism and rate of limited proteolytic cleavage of IgD and also to identify, isolate, and quantify the reaction p...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.1.61

    authors： Ishioka N,Takahashi N,Putnam FW

    更新日期：1987-01-01 00:00:00

  • Precise detection of de novo single nucleotide variants in human genomes.

    abstract：:The precise determination of de novo genetic variants has enormous implications across different fields of biology and medicine, particularly personalized medicine. Currently, de novo variations are identified by mapping sample reads from a parent-offspring trio to a reference genome, allowing for a certain degree of ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1802244115

    authors： Gómez-Romero L,Palacios-Flores K,Reyes J,García D,Boege M,Dávila G,Flores M,Schatz MC,Palacios R

    更新日期：2018-05-22 00:00:00

  • Critical role for phosphoinositide 3-kinase gamma in parasite invasion and disease progression of cutaneous leishmaniasis.

    abstract：:Obligate intracellular pathogens such as Leishmania specifically target host phagocytes for survival and replication. Phosphoinositide 3-kinase γ (PI3Kγ), a member of the class I PI3Ks that is highly expressed by leukocytes, controls cell migration by initiating actin polymerization and cytoskeletal reorganization, wh...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1110339109

    authors： Cummings HE,Barbi J,Reville P,Oghumu S,Zorko N,Sarkar A,Keiser TL,Lu B,Rückle T,Varikuti S,Lezama-Davila C,Wewers MD,Whitacre C,Radzioch D,Rommel C,Seveau S,Satoskar AR

    更新日期：2012-01-24 00:00:00

  • Hatchling turtles survive freezing during winter hibernation.

    abstract：:Hatchlings of the painted turtle (Chrysemys picta marginata) are unique as the only reptile and highest vertebrate life form known to tolerate the natural freezing of extracellular body fluids during winter hibernation. Turtles survived frequent exposures to temperatures as low as -6 degrees C to -8 degrees C in their...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.21.8350

    authors： Storey KB,Storey JM,Brooks SP,Churchill TA,Brooks RJ

    更新日期：1988-11-01 00:00:00

  • Surface photometry of celestial sources from a space vehicle: introduction and observational procedures.

    abstract：:Diffuse celestial sources of relatively low surface brightness such as the Milky Way, zodiacal light, and gegenschein (or contre lumière) can be studied most reliably from above the earth's atmosphere with equipment flown in artificial satellites. We review the techniques used and some of the difficulties encountered ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.69.3.694

    authors： Roach FE,Carroll B,Aller LH,Smith L

    更新日期：1972-03-01 00:00:00

  • Transmembrane E3 ligase RNF183 mediates ER stress-induced apoptosis by degrading Bcl-xL.

    abstract：:The accumulation of misfolded proteins in the endoplasmic reticulum (ER) causes ER stress and triggers the unfolded protein response (UPR). Failure to resolve ER stress leads to apoptotic cell death via a yet unclear mechanism. Here, we show that RNF183, a membrane-spanning RING finger protein, localizes to the ER and...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1716439115

    authors： Wu Y,Li X,Jia J,Zhang Y,Li J,Zhu Z,Wang H,Tang J,Hu J

    更新日期：2018-03-20 00:00:00

  • Calcification in atherosclerosis: bone biology and chronic inflammation at the arterial crossroads.

    abstract：:Dystrophic or ectopic mineral deposition occurs in many pathologic conditions, including atherosclerosis. Calcium mineral deposits that frequently accompany atherosclerosis are readily quantifiable radiographically, serve as a surrogate marker for the disease, and predict a higher risk of myocardial infarction and dea...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1932554100

    authors： Doherty TM,Asotra K,Fitzpatrick LA,Qiao JH,Wilkin DJ,Detrano RC,Dunstan CR,Shah PK,Rajavashisth TB

    更新日期：2003-09-30 00:00:00

  • Social and general intelligence improves collective action in a common pool resource system.

    abstract：:On a planet experiencing global environmental change, the governance of natural resources depends on sustained collective action by diverse populations. Engaging in such collective action can only build upon the foundation of human cognition in social-ecological settings. To help understand this foundation, we assess ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1915824117

    authors： Freeman J,Baggio JA,Coyle TR

    更新日期：2020-04-07 00:00:00

  • The Candida albicans KRE9 gene is required for cell wall beta-1, 6-glucan synthesis and is essential for growth on glucose.

    abstract：:We have isolated CaKRE9, a gene from Candida albicans, that is a functional homologue of the Saccharomyces cerevisiae KRE9 gene involved in beta-1,6-glucan synthesis. Disruption of the CaKRE9 gene in C. albicans shows that CaKre9p is required for the synthesis or assembly of this fungal polymer. Homozygous null disrup...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.17.9825

    authors： Lussier M,Sdicu AM,Shahinian S,Bussey H

    更新日期：1998-08-18 00:00:00

  • Antibodies against membrane interleukin 1 alpha activate accessory cells to stimulate proliferation of T lymphocytes.

    abstract：:Some monoclonal antibodies (mAbs) against interleukin (IL) 1 alpha have been found to activate antigen-presenting cells (APC, human peripheral blood monocytes and B lymphocytes), so that unstimulated T lymphocytes cultured with them are induced to proliferate and secrete IL-2. Control mAbs of the same isotypes and mAb...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.4.1305

    authors： Eugui EM,Almquist SJ

    更新日期：1990-02-01 00:00:00

  • The molecular weight of the major glycoprotein from the human erythrocyte membrane.

    abstract：:The molecular weight of the major glycoprotein from the human erythrocyte membrane is 29,000, of which 55% is carbohydrate and 45% is protein. The binding of sodium dodecyl sulfate to this glycoprotein is anomalous when compared to water soluble proteins and leads to migration rates in sodium dodecyl sulfate-polyacryl...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.71.10.3913

    authors： Grefrath SP,Reynolds JA

    更新日期：1974-10-01 00:00:00

  • Nanoscale imaging reveals laterally expanding antimicrobial pores in lipid bilayers.

    abstract：:Antimicrobial peptides are postulated to disrupt microbial phospholipid membranes. The prevailing molecular model is based on the formation of stable or transient pores although the direct observation of the fundamental processes is lacking. By combining rational peptide design with topographical (atomic force microsc...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1222824110

    authors： Rakowska PD,Jiang H,Ray S,Pyne A,Lamarre B,Carr M,Judge PJ,Ravi J,Gerling UI,Koksch B,Martyna GJ,Hoogenboom BW,Watts A,Crain J,Grovenor CR,Ryadnov MG

    更新日期：2013-05-28 00:00:00

  • Deamidation of human proteins.

    abstract：:Deamidation of asparaginyl and glutaminyl residues causes time-dependent changes in charge and conformation of peptides and proteins. Quantitative and experimentally verified predictive calculations of the deamidation rates of 1,371 asparaginyl residues in a representative collection of 126 human proteins have been pe...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.221463198

    authors： Robinson NE,Robinson AB

    更新日期：2001-10-23 00:00:00

  • Structural insights into tail-anchored protein binding and membrane insertion by Get3.

    abstract：:Tail-anchored (TA) membrane proteins are involved in a variety of important cellular functions, including membrane fusion, protein translocation, and apoptosis. The ATPase Get3 (Asna1, TRC40) was identified recently as the endoplasmic reticulum targeting factor of TA proteins. Get3 consists of an ATPase and alpha-heli...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0910223106

    authors： Bozkurt G,Stjepanovic G,Vilardi F,Amlacher S,Wild K,Bange G,Favaloro V,Rippe K,Hurt E,Dobberstein B,Sinning I

    更新日期：2009-12-15 00:00:00

  • A neuronal signature for monogamous reunion.

    abstract：:Pair-bond formation depends vitally on neuromodulatory signaling within the nucleus accumbens, but the neuronal dynamics underlying this behavior remain unclear. Using 1-photon in vivo Ca2+ imaging in monogamous prairie voles, we found that pair bonding does not elicit differences in overall nucleus accumbens Ca2+ act...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1917287117

    authors： Scribner JL,Vance EA,Protter DSW,Sheeran WM,Saslow E,Cameron RT,Klein EM,Jimenez JC,Kheirbek MA,Donaldson ZR

    更新日期：2020-05-19 00:00:00

  • Direct demonstration of the isomerization component of the monoterpene cyclase reaction using a cyclopropylcarbinyl pyrophosphate substrate analog.

    abstract：:The tightly coupled nature of the reaction sequence catalyzed by monoterpene cyclases has precluded direct observation of the topologically required isomerization step leading from geranyl pyrophosphate to the presumptive, enzyme-bound, tertiary allylic intermediate linalyl pyrophosphate, which ultimately cyclizes to ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.14.4856

    authors： Wheeler CJ,Croteau RB

    更新日期：1987-07-01 00:00:00

  • Effect of electron count and chemical complexity in the Ta-Nb-Hf-Zr-Ti high-entropy alloy superconductor.

    abstract：:High-entropy alloys are made from random mixtures of principal elements on simple lattices, stabilized by a high mixing entropy. The recently discovered body-centered cubic (BCC) Ta-Nb-Hf-Zr-Ti high-entropy alloy superconductor appears to display properties of both simple crystalline intermetallics and amorphous mater...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1615926113

    authors： von Rohr F,Winiarski MJ,Tao J,Klimczuk T,Cava RJ

    更新日期：2016-11-15 00:00:00

  • Dengue reporter viruses reveal viral dynamics in interferon receptor-deficient mice and sensitivity to interferon effectors in vitro.

    abstract：:Dengue virus (DENV) is a global disease threat for which there are no approved antivirals or vaccines. Establishing state-of-the-art screening systems that rely on fluorescent or luminescent reporters may accelerate the development of anti-DENV therapeutics. However, relatively few reporter DENV platforms exist. Here,...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1212379109

    authors： Schoggins JW,Dorner M,Feulner M,Imanaka N,Murphy MY,Ploss A,Rice CM

    更新日期：2012-09-04 00:00:00

  • Isolation and characterization of a T-lymphocyte somatic mutant with altered signal transduction by the antigen receptor.

    abstract：:We have developed an approach for deriving and characterizing antigen-receptor (CD3/Ti) signal-transduction mutants. This strategy combines receptor-mediated growth inhibition and fluorescence-activated cell sorting with the Ca2+-indicator indo-1. Despite the expression of structurally normal CD3/Ti complexes, one suc...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.19.6879

    authors： Goldsmith MA,Weiss A

    更新日期：1987-10-01 00:00:00

  • A labile inhibitor blocks immunoglobulin kappa-light-chain-gene transcription in a pre-B leukemic cell line.

    abstract：:The murine pre-B leukemic cell line 70Z/3 contains both an unrearranged immunoglobulin kappa-light-chain gene and a functionally rearranged but silent kappa-light-chain gene. Mitogenic stimulation of growing 70Z/3 cells with bacterial lipopolysaccharide (LPS) activates kappa-light-gene transcription and results in a 1...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.83.2.295

    authors： Wall R,Briskin M,Carter C,Govan H,Taylor A,Kincade P

    更新日期：1986-01-01 00:00:00

  • Molecular cloning of the neu gene: absence of gross structural alteration in oncogenic alleles.

    abstract：:The neu gene is distantly related to the erbB gene and encodes a cell surface protein that appears to function as a growth factor receptor. To study the mechanisms that caused the conversion of the normal neu gene to an oncogenic allele, we have isolated molecular clones of the neu oncogene as well as a clone of the c...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.83.2.261

    authors： Hung MC,Schechter AL,Chevray PY,Stern DF,Weinberg RA

    更新日期：1986-01-01 00:00:00

  • Reversible redistribution of phytochrome within the cell upon conversion to its physiologically active form.

    abstract：:The intracellular localization of phytochrome was seen in dark-grown oat (Avena sativa L., cv. Garry) and rice (Oryza sativa L., cv. unknown) shoots after various light treatments using an indirect peroxidase-antiperoxidase antibody labeling method. Phytochrome is generally distributed throughout the cytoplasm in cell...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.72.3.799

    authors： Mackenzie JM Jr,Coleman RA,Briggs WR,Pratt LH

    更新日期：1975-03-01 00:00:00

  • High-level expression of a heterologous protein in the milk of transgenic swine using the cDNA encoding human protein C.

    abstract：:Transgenic pigs were generated that produced human protein C in their milk at up to 1 g/liter. The gene construct was a fusion gene consisting of the cDNA for human protein C inserted into the first exon of the mouse whey acidic protein gene. These results demonstrate that the mouse whey acidic protein gene contains r...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.24.12003

    authors： Velander WH,Johnson JL,Page RL,Russell CG,Subramanian A,Wilkins TD,Gwazdauskas FC,Pittius C,Drohan WN

    更新日期：1992-12-15 00:00:00

  • Ferulate-5-hydroxylase from Arabidopsis thaliana defines a new family of cytochrome P450-dependent monooxygenases.

    abstract：:The fah1 mutant of Arabidopsis is defective in the accumulation of sinapic acid-derived metabolites, including the guaiacyl-syringyl lignin typical of angiosperms. Earlier results indicated that the FAH1 locus encodes ferulate-5-hydroxylase (F5H), a cytochrome P450-dependent monooxygenase (P450) of the general phenylp...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.14.6869

    authors： Meyer K,Cusumano JC,Somerville C,Chapple CC

    更新日期：1996-07-09 00:00:00

  • Selection of distinct Hox-Extradenticle interaction modes fine-tunes Hox protein activity.

    abstract：:Hox genes encode transcription factors widely used for diversifying animal body plans in development and evolution. To achieve functional specificity, Hox proteins associate with PBC class proteins, Pre-B cell leukemia homeobox (Pbx) in vertebrates, and Extradenticle (Exd) in Drosophila, and were thought to use a uniq...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1006964108

    authors： Saadaoui M,Merabet S,Litim-Mecheri I,Arbeille E,Sambrani N,Damen W,Brena C,Pradel J,Graba Y

    更新日期：2011-02-08 00:00:00

  • The role of carbonic anhydrases in extinction of contextual fear memory.

    abstract：:Carbonic anhydrases (CAs; EC 4.2.1.1) are metalloenzymes present in mammals with 16 isoforms that differ in terms of catalytic activity as well as cellular and tissue distribution. CAs catalyze the conversion of CO2 to bicarbonate and protons and are involved in various physiological processes, including learning and ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1910690117

    authors： Schmidt SD,Costa A,Rani B,Godfried Nachtigall E,Passani MB,Carta F,Nocentini A,de Carvalho Myskiw J,Furini CRG,Supuran CT,Izquierdo I,Blandina P,Provensi G

    更新日期：2020-07-07 00:00:00

  • Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading frame BCRFI.

    abstract：:We have demonstrated the existence of human cytokine synthesis inhibitory factor (CSIF) [interleukin 10 (IL-10)]. cDNA clones encoding human IL-10 (hIL-10) were isolated from a tetanus toxin-specific human T-cell clone. Like mouse IL-10, hIL-10 exhibits strong DNA and amino acid sequence homology to an open reading fr...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.4.1172

    authors： Vieira P,de Waal-Malefyt R,Dang MN,Johnson KE,Kastelein R,Fiorentino DF,deVries JE,Roncarolo MG,Mosmann TR,Moore KW

    更新日期：1991-02-15 00:00:00

  • Reconstitution of intramembrane proteolysis in vitro reveals that pure rhomboid is sufficient for catalysis and specificity.

    abstract：:Intramembrane proteolysis is a new paradigm in biology that controls signaling events throughout evolution. Hydrolysis of peptide bonds is thought to occur within the normally hydrophobic membrane environment, but insights into this unusual activity have been lacking because of difficulty in recapitulating activity in...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0408306102

    authors： Urban S,Wolfe MS

    更新日期：2005-02-08 00:00:00

  • Primarily chronic progressive and relapsing/remitting multiple sclerosis: two immunogenetically distinct disease entities.

    abstract：:HLA class II gene polymorphism was investigated in 100 patients with clinically definite multiple sclerosis (MS) by restriction fragment length polymorphism analysis of Taq I-digested DNA using DRB, DQA, and DQB cDNA probes. Twenty-six patients had primarily chronic progressive MS and 74 had relapsing/remitting MS. Th...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.18.7113

    authors： Olerup O,Hillert J,Fredrikson S,Olsson T,Kam-Hansen S,Möller E,Carlsson B,Wallin J

    更新日期：1989-09-01 00:00:00

  • Discovery of novel bacterial queuine salvage enzymes and pathways in human pathogens.

    abstract：:Queuosine (Q) is a complex tRNA modification widespread in eukaryotes and bacteria that contributes to the efficiency and accuracy of protein synthesis. Eukaryotes are not capable of Q synthesis and rely on salvage of the queuine base (q) as a Q precursor. While many bacteria are capable of Q de novo synthesis, salvag...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1909604116

    authors： Yuan Y,Zallot R,Grove TL,Payan DJ,Martin-Verstraete I,Šepić S,Balamkundu S,Neelakandan R,Gadi VK,Liu CF,Swairjo MA,Dedon PC,Almo SC,Gerlt JA,de Crécy-Lagard V

    更新日期：2019-09-17 00:00:00