Analysis of the mechanism, rate, and sites of proteolytic cleavage of human immunoglobulin D by high-pressure liquid chromatography.

abstract：:The outcome of exposure to infectious microbes or their toxins is influenced by both microbial and host genes. Some host genes encode defense mechanisms, whereas others assist pathogen functions. Genomic analyses have associated host gene mutations with altered infectious disease susceptibility, but evidence for causa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Martchenko M,Candille SI,Tang H,Cohen SN

更新日期：2012-02-21 00:00:00

abstract：:Two anti-tumor monoclonal antibodies, L6 (anticarcinoma) and 1F5 (anti-B lymphoma), were covalently linked to alkaline phosphatase (AP), forming conjugates that could bind to the surface of antigen-positive tumor cells. The conjugates were capable of converting a relatively noncytotoxic prodrug, etoposide phosphate (E...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Senter PD,Saulnier MG,Schreiber GJ,Hirschberg DL,Brown JP,Hellström I,Hellström KE

更新日期：1988-07-01 00:00:00

abstract：:Saccharomyces cerevisiae strain PP5 has a phosphodiesterase (PDE) deficiency that results in heat-shock sensitivity due to the intracellular accumulation of cAMP. This strain also carries the cam mutation, which confers permeability to cAMP and, as shown here, to other compounds. Expression of rat type IV PDE in these...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pillai R,Kytle K,Reyes A,Colicelli J

更新日期：1993-12-15 00:00:00

abstract：:The clonal rat pituitary cell line GH4C1 expresses the genes for several voltage-dependent potassium channels including Kv1.5 and Kv1.4. Dexamethasone, a glucocorticoid agonist, induces a slowly inactivating potassium current in these cells but does not alter the amplitude of a rapidly inactivating component of potass...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chung S,Saal DB,Kaczmarek LK

更新日期：1995-06-20 00:00:00

abstract：:Maintenance of genome stability is essential for the accurate propagation of genetic information and cell growth and survival. Organisms have therefore developed efficient strategies to prevent DNA lesions and rearrangements. Much of the information concerning these strategies has been obtained through the study of ba...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maréchal A,Parent JS,Véronneau-Lafortune F,Joyeux A,Lang BF,Brisson N

更新日期：2009-08-25 00:00:00

abstract：:Physical activity increases energy metabolism in exercising muscle. Whether acute exercise elicits metabolic changes in nonexercising muscles remains unclear. We show that one of the few genes that is more highly induced in nonexercising muscle than in exercising human muscle during acute exercise encodes angiopoietin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Catoire M,Alex S,Paraskevopulos N,Mattijssen F,Evers-van Gogh I,Schaart G,Jeppesen J,Kneppers A,Mensink M,Voshol PJ,Olivecrona G,Tan NS,Hesselink MK,Berbée JF,Rensen PC,Kalkhoven E,Schrauwen P,Kersten S

更新日期：2014-03-18 00:00:00

abstract：:Cloned DNA templates were used to direct the transcription of early and late simian virus 40 (SV40) genes by a cell-free RNA-synthesizing system. Transcription by RNA polymerase II was sensitive to low levels of alpha-amanitin and completely dependent on exogenously added DNA template. RNA products of discrete lengths...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rio D,Robbins A,Myers R,Tjian R

更新日期：1980-10-01 00:00:00

abstract：:Antibody raised against the human erythrocyte glucose transporter identified a recombinant lambda gt11 bacteriophage in a cDNA library prepared from immunoselected polysomal RNA from adult rat brain. The cDNA predicts a 492-amino acid protein that demonstrates 97.6% identity to the human hepatoma hexose carrier. The t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Birnbaum MJ,Haspel HC,Rosen OM

更新日期：1986-08-01 00:00:00

abstract：:The mouse mammary tumor virus (MMTV) promoter is regulated by steroid hormones through a hormone-responsive region that is organized in a positioned nucleosome. Hormone induction leads to a structural change of this nucleosome which makes its DNA more sensitive to cleavage by DNase I and enables simultaneous binding o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bartsch J,Truss M,Bode J,Beato M

更新日期：1996-10-01 00:00:00

abstract：:Spatial memory enhances an organism's navigational ability. Memory typically resides within the brain, but what if an organism has no brain? We show that the brainless slime mold Physarum polycephalum constructs a form of spatial memory by avoiding areas it has previously explored. This mechanism allows the slime mold...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reid CR,Latty T,Dussutour A,Beekman M

更新日期：2012-10-23 00:00:00

abstract：:The interaction of the NH2-terminal DNA-binding domain of lac repressor with synthetic oligo[d(AT)] was studied by a photo-CIDNP technique (CIDNP is chemically induced dynamic nuclear polarization). Three of the four tyrosines of the NH2-terminal region were found to be accessible to the photosensitive dye. The corres...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Buck F,Rüterjans H,Kaptein R,Beyreuther K

更新日期：1980-09-01 00:00:00

abstract：:Over the past decade or more, contradictory evidence of Martian climate, indicating that surface temperatures seldom if ever approach the melting point of water at midlatitudes, and geomorphic features, consistent with liquid flows at these same latitudes, have proven difficult to reconcile. In this article, we demons...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shinbrot T,Duong NH,Kwan L,Alvarez MM

更新日期：2004-06-08 00:00:00

abstract：:New neurons are continuously generated in restricted regions of the adult mammalian brain. Although these adult-born neurons have been shown to receive synaptic inputs, little is known about their synaptic outputs. Using retrovirus-mediated birth-dating and labeling in combination with serial section electron microsco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Faulkner RL,Jang MH,Liu XB,Duan X,Sailor KA,Kim JY,Ge S,Jones EG,Ming GL,Song H,Cheng HJ

更新日期：2008-09-16 00:00:00

abstract：:Insertion of trocars, needles, and catheters into unintended tissues or tissue compartments results in hundreds of thousands of complications annually. Current methods for blood vessel cannulation or epidural, chest tube, and initial trocar placement often involve the blind pass of a needle through several layers of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bassett EK,Slocum AH,Masiakos PT,Pryor HI 2nd,Farokhzad OC,Karp JM

更新日期：2009-04-07 00:00:00

abstract：:Physical biochemical techniques are used to establish the structure, subunit stoichiometry, and assembly pathway of the primosome complex of the bacteriophage T4 DNA replication system. Analytical ultracentrifugation and fluorescence anisotropy methods show that the functional T4 primosome consists of six gp41 helicas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jose D,Weitzel SE,Jing D,von Hippel PH

更新日期：2012-08-21 00:00:00

abstract：:Lipid rafts are important signaling platforms in T cells. Little is known about their properties in human CD8(+) T cells. We studied polarization of lipid rafts by digital immunofluorescence microscopy in primary human T cells, using beads coated with anti-CD3 and anti-CD28 mAbs (CD3/28 beads). Unlike CD4(+) T cells, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kovacs B,Maus MV,Riley JL,Derimanov GS,Koretzky GA,June CH,Finkel TH

更新日期：2002-11-12 00:00:00

abstract：:The interaction of human high density lipoproteins (HDL) with isolated human monocytes from control and Tangier patients was studied in tissue culture experiments. It was observed that normal monocytes, similar to mouse peritoneal macrophages, bind HDL to a cell surface receptor, internalize the bound HDL particles, t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schmitz G,Assmann G,Robenek H,Brennhausen B

更新日期：1985-09-01 00:00:00

abstract：:Viral genome packaging into capsids is powered by high-force-generating motor proteins. In the presence of all packaging components, ATP-powered translocation in vitro expels all detectable tightly bound YOYO-1 dye from packaged short dsDNA substrates and removes all aminoacridine dye from packaged genomic DNA in vivo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dixit AB,Ray K,Black LW

更新日期：2012-12-11 00:00:00

abstract：:New geochemical data from the Malawi Rift (Chiwondo Beds, Karonga Basin) fill a major spatial gap in our knowledge of hominin adaptations on a continental scale. Oxygen (δ18O), carbon (δ13C), and clumped (Δ47) isotope data on paleosols, hominins, and selected fauna elucidate an unexpected diversity in the Pleistocene ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lüdecke T,Kullmer O,Wacker U,Sandrock O,Fiebig J,Schrenk F,Mulch A

更新日期：2018-12-26 00:00:00

abstract：:Various tissues and cells in culture contain a specific inhibitor of DNase I (EC 3.1.4.5). In this paper evidence is presented that this inhibitor is actin, one of the major structural proteins of muscle and nonmuscle cells. (a) The inhibitor is a major cellular component constituting 5-10% of the soluble protein. (b)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lazarides E,Lindberg U

更新日期：1974-12-01 00:00:00

abstract：:Focal electric stimulation of the auditory cortex is well suited for exploration of the function of the corticofugal (descending) system and the neural mechanism of plasticity in the central auditory system, because it evokes changes in frequency-tuning, called best frequency (BF) shifts, as does auditory fear conditi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xiao Z,Suga N

更新日期：2005-12-27 00:00:00

abstract：:Biological organisms have evolved a wide range of immune mechanisms to defend themselves against pathogens. Beyond molecular details, these mechanisms differ in how protection is acquired, processed, and passed on to subsequent generations-differences that may be essential to long-term survival. Here, we introduce a m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mayer A,Mora T,Rivoire O,Walczak AM

更新日期：2016-08-02 00:00:00

abstract：:It was shown in an earlier paper how to connect, in principle, the biochemical states of a cross-bridge with the mechanics of muscular contraction, by the methods of statistical mechanics. The treatment applies to cross-bridges that are able to interact with only one actin site at a time. The present paper shows that ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hill TL

更新日期：1973-10-01 00:00:00

abstract：:Methotrexate (MTX) conjugated to a Mr 3000 poly(L-Lys) markedly inhibits the growth of Pro-3 MtxRII5-3 Chinese hamster ovarian cells, a mutant line known to be drug resistant because of defective MTX transport. In these cells, membrane transport of [3H]MTX-poly(Lys) is sharply decreased by addition of 0.5- to 2.5-fold...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shen WC,Ryser HJ

更新日期：1981-12-01 00:00:00

abstract：:Familial adenomatous polyposis (FAP) is an autosomal-dominant disease characterized by the development of hundreds of adenomatous polyps of the colorectum. Approximately 80% of FAP patients can be shown to have truncating mutations of the APC gene. To determine the cause of FAP in the other 20% of patients, MAMA (mono...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Laken SJ,Papadopoulos N,Petersen GM,Gruber SB,Hamilton SR,Giardiello FM,Brensinger JD,Vogelstein B,Kinzler KW

更新日期：1999-03-02 00:00:00

abstract：:We study the dynamic network of e-mail traffic and find that it develops self-organized coherent structures similar to those appearing in many nonlinear dynamic systems. Such structures are uncovered by a general information theoretic approach to dynamic networks based on the analysis of synchronization among trios of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eckmann JP,Moses E,Sergi D

更新日期：2004-10-05 00:00:00

abstract：:In this era of continued emergence of zoonotic virus infections, the rapid development of rodent models represents a critical barrier to public health preparedness, including the testing of antivirus therapy and vaccines. The Middle East respiratory syndrome coronavirus (MERS-CoV) was recently identified as the causat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao J,Li K,Wohlford-Lenane C,Agnihothram SS,Fett C,Zhao J,Gale MJ Jr,Baric RS,Enjuanes L,Gallagher T,McCray PB Jr,Perlman S

更新日期：2014-04-01 00:00:00

abstract：:Somatic crossing over in flies heterozygous for a dominant Minute mutant may result in two cells, homozygous for wild type and Minute, respectively. If both cells took part in development, a twin spot of two genotypes, different from the rest of the body, would result. Two-hundred wild type spots were found, none of w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stern C,Tokunaga C

更新日期：1971-02-01 00:00:00

abstract：:A full-length cDNA clone encoding a purified augmenter of liver regeneration (ALR) factor prepared from the cytosol of weanling rat livers was isolated. The 1.2-kb cDNA included a 299-bp 5' untranslated region, a 375-bp coding region, and a 550-bp 3' untranslated region. It encoded a protein consisting of 125 amino ac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hagiya M,Francavilla A,Polimeno L,Ihara I,Sakai H,Seki T,Shimonishi M,Porter KA,Starzl TE

更新日期：1994-08-16 00:00:00

abstract：:Primary--i.e., nonsyndromal-postlingual deafness is inherited as an autosomal dominant phenotype in a large kindred in Costa Rica. Genetically susceptible individuals begin to lose hearing at low frequencies at about age 10 years, after language and speaking are learned. Deafness inevitably progresses by age 30 years ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leon PE,Raventos H,Lynch E,Morrow J,King MC

更新日期：1992-06-01 00:00:00