Use of a yeast expression system for the isolation and analysis of drug-resistant mutants of a mammalian phosphodiesterase.

abstract：:The sense of hearing is remarkable for its auditory dynamic range, which spans more than 10(12) in acoustic intensity. The mechanisms that enable the cochlea to transduce high sound levels without damage are of key interest, particularly with regard to the broad impact of industrial, military, and recreational auditor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Housley GD,Morton-Jones R,Vlajkovic SM,Telang RS,Paramananthasivam V,Tadros SF,Wong AC,Froud KE,Cederholm JM,Sivakumaran Y,Snguanwongchai P,Khakh BS,Cockayne DA,Thorne PR,Ryan AF

更新日期：2013-04-30 00:00:00

abstract：:Protective immunity against human schistosome infection develops slowly, for reasons that are not yet fully understood. For many decades, researchers have attempted to infer properties of the immune response from epidemiological studies, with mathematical models frequently being used to bridge the gap between immunolo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitchell KM,Mutapi F,Savill NJ,Woolhouse ME

更新日期：2012-08-14 00:00:00

abstract：:Two-dimensional electron gases (2DEGs) are at the base of current nanoelectronics because of their exceptional mobilities. Often the accumulation layer forms at polar interfaces with longitudinal optical (LO) modes. In most cases, the many-body screening of the quasi-2DEGs dramatically reduces the Fröhlich scattering ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen Z,Sjakste J,Dong J,Taleb-Ibrahimi A,Rueff JP,Shukla A,Peretti J,Papalazarou E,Marsi M,Perfetti L

更新日期：2020-09-08 00:00:00

abstract：:Ferric uptake regulation protein (Fur) is a bacterial global regulator that uses iron as a cofactor to bind to specific DNA sequences. The function of Fur is not limited to iron homeostasis. A wide variety of genes involved in various mechanisms such as oxidative and acid stresses are under Fur control. Flavohemoglobi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： D'Autreaux B,Touati D,Bersch B,Latour JM,Michaud-Soret I

更新日期：2002-12-24 00:00:00

abstract：:A vitamin D(3) metabolite in intestine more polar than 25-hydroxy-vitamin D(3)(25-OH D(3)) has been detected by countercurrent distribution. The intestinal metabolite is found also after administration of labeled hydroxy D(3), indicating that it arises from vitamin D(3) via the intermediate 25-hydroxy derivative. The ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haussler MR,Boyce DW,Littledike ET,Rasmussen H

更新日期：1971-01-01 00:00:00

abstract：:Physical forces have a profound effect on growth, morphology, locomotion, and survival of organisms. At the level of individual cells, the role of mechanical forces is well recognized in eukaryotic physiology, but much less is known about prokaryotic organisms. Recent findings suggest an effect of physical forces on b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Genova LA,Roberts MF,Wong YC,Harper CE,Santiago AG,Fu B,Srivastava A,Jung W,Wang LM,Krzemiński Ł,Mao X,Sun X,Hui CY,Chen P,Hernandez CJ

更新日期：2019-12-17 00:00:00

abstract：:The INK4a-ARF locus encodes two proteins, p16(INK4a) and p19(ARF), that restrain cell growth by affecting the functions of the retinoblastoma protein and p53, respectively. Disruption of this locus by deletions or point mutations is a common event in human cancer, perhaps second only to the loss of p53. Using insect c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kamijo T,Weber JD,Zambetti G,Zindy F,Roussel MF,Sherr CJ

更新日期：1998-07-07 00:00:00

abstract：:Utilizing the bicistronic reporter transposon mini-Tn5 lacZ-tet/1, we have identified lacZ fusions to four Escherichia coli genes/operons that are strongly activated by the accumulation of self-produced extracellular signals. These fusions were designated cma9, cma48, cma113, and cma114 for conditioned medium activate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baca-DeLancey RR,South MM,Ding X,Rather PN

更新日期：1999-04-13 00:00:00

abstract：:Considerable effort has been directed toward controlling tuberculosis, which kills almost two million people yearly. High on the research agenda is the discovery of biomarkers of active tuberculosis (TB) for diagnosis and for monitoring treatment outcome. Rational biomarker discovery requires understanding host-pathog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kunnath-Velayudhan S,Salamon H,Wang HY,Davidow AL,Molina DM,Huynh VT,Cirillo DM,Michel G,Talbot EA,Perkins MD,Felgner PL,Liang X,Gennaro ML

更新日期：2010-08-17 00:00:00

abstract：:The need to assess subtle, potentially exploitable changes in serial structure is paramount in the analysis of financial data. Herein, we demonstrate the utility of approximate entropy (ApEn), a model-independent measure of sequential irregularity, toward this goal, by several distinct applications. We consider both e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pincus S,Kalman RE

更新日期：2004-09-21 00:00:00

abstract：:The present study examined the social skills of previously institutionalized, 8-y-old Romanian children from the Bucharest Early Intervention Project and the influence of attachment security and brain electrical activity (alpha power) on these skills. Participants included children randomized to an intervention involv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Almas AN,Degnan KA,Radulescu A,Nelson CA 3rd,Zeanah CH,Fox NA

更新日期：2012-10-16 00:00:00

abstract：:The fossil record of land plants is an obvious source of information on the dynamics of mass extinctions in the geological past. In conjunction with the end-Permian ecological crisis, approximately 250 million years ago, palynological data from East Greenland reveal some unanticipated patterns. We document the signifi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Looy CV,Twitchett RJ,Dilcher DL,Van Konijnenburg-Van Cittert JH,Visscher H

更新日期：2001-07-03 00:00:00

abstract：:Flagellar polypeptides of Chlamydomonas reinhardtii were analyzed in two-dimensions by isoelectric focusing and electrophoresis in the presence of sodium dodecyl sulfate. In addition to flagellar tubulin, over 130 polypeptides were resolved and 100 of these were identified as axonemal components in wild-type organisms...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Piperno G,Huang B,Luck DJ

更新日期：1977-04-01 00:00:00

abstract：:Acetyl-CoA carboxylase 1 (ACC1) catalyzes the formation of malonyl-CoA, the C2 donor for de novo synthesis of long-chain fatty acids. We have identified 64 exons, including 7 alternatively spliced minor exons (1A, 1B, 1C, 3, 5A', 5A, and 5B) in human ACC1 gene ( approximately 330 kb). The gene is regulated by three pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mao J,Chirala SS,Wakil SJ

更新日期：2003-06-24 00:00:00

abstract：:Transcriptional activation by steroid hormones is often associated with the appearance of a DNase I hypersensitive site resulting from a local alteration of the nucleoprotein structure of the promoter. For the mouse mammary tumor virus long terminal repeat, a viral promoter under glucocorticoid control, a model has be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carr KD,Richard-Foy H

更新日期：1990-12-01 00:00:00

abstract：:Economic experiments are often used to study if humans altruistically value the welfare of others. A canonical result from public-good games is that humans vary in how they value the welfare of others, dividing into fair-minded conditional cooperators, who match the cooperation of others, and selfish noncooperators. H...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Burton-Chellew MN,El Mouden C,West SA

更新日期：2016-02-02 00:00:00

abstract：:The challenging problem of ultra-high-energy-density, high-efficiency, and small-scale portable power generation is addressed here using a distinctive thermophotovoltaic energy conversion mechanism and chip-based system design, which we name the microthermophotovoltaic (μTPV) generator. The approach is predicted to be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chan WR,Bermel P,Pilawa-Podgurski RC,Marton CH,Jensen KF,Senkevich JJ,Joannopoulos JD,Soljacic M,Celanovic I

更新日期：2013-04-02 00:00:00

abstract：:A critical step in neuronal development is the formation of axon/dendrite polarity, a process involving symmetry breaking in the newborn neuron. Local self-amplifying processes could enhance and stabilize the initial asymmetry in the distribution of axon/dendrite determinants, but the identity of these processes remai...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheng PL,Song AH,Wong YH,Wang S,Zhang X,Poo MM

更新日期：2011-11-08 00:00:00

abstract：:Since the demonstration that the sequence of a protein encodes its structure, the prediction of structure from sequence remains an outstanding problem that impacts numerous scientific disciplines, including many genome projects. By iteratively fixing secondary structure assignments of residues during Monte Carlo simul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： DeBartolo J,Colubri A,Jha AK,Fitzgerald JE,Freed KF,Sosnick TR

更新日期：2009-03-10 00:00:00

abstract：:Tauopathies are a class of neurodegenerative diseases associated with pathological tau. Despite many advances in our understanding of these diseases, the direct mechanism through which tau contributes to neurodegeneration remains poorly understood. Previously, our laboratory implicated the histone demethylase LSD1 in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Engstrom AK,Walker AC,Moudgal RA,Myrick DA,Kyle SM,Bai Y,Rowley MJ,Katz DJ

更新日期：2020-11-17 00:00:00

abstract：:Human neutrophils respond to chemoattractants, resulting in their accumulation at an inflammatory site. Chemoattractants such as the C5a peptide, derived from the C5 complement factor, bind to inhibitory guanine nucleotide binding protein (Gi)-coupled seven membrane-spanning receptors expressed in neutrophils. C5a rec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Buhl AM,Avdi N,Worthen GS,Johnson GL

更新日期：1994-09-13 00:00:00

abstract：:To investigate the evolution of the mammalian sex chromosomes, we have compared the gene content of the X chromosomes in the mammalian groups most distantly related to man (marsupials and monotremes). Previous work established that genes on the long arm of the human X chromosome are conserved on the X chromosomes in a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Watson JM,Spencer JA,Riggs AD,Graves JA

更新日期：1991-12-15 00:00:00

abstract：:A hallmark of the pathology of Alzheimer's disease is the accumulation of the microtubule-associated protein tau into fibrillar aggregates. Recent studies suggest that they accumulate because cytosolic chaperones fail to clear abnormally phosphorylated tau, preserving a pool of toxic tau intermediates within the neuro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dickey CA,Koren J,Zhang YJ,Xu YF,Jinwal UK,Birnbaum MJ,Monks B,Sun M,Cheng JQ,Patterson C,Bailey RM,Dunmore J,Soresh S,Leon C,Morgan D,Petrucelli L

更新日期：2008-03-04 00:00:00

abstract：:Stochasticity alters the nonlinear dynamics of inherently cycling populations. The power spectrum can describe and explain the impacts of stochasticity. We fitted models to short observed time series of flour beetle populations in the frequency domain, then used a well fitting stochastic mechanistic model to generate ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reuman DC,Desharnais RA,Costantino RF,Ahmad OS,Cohen JE

更新日期：2006-12-05 00:00:00

abstract：:3-Methylcholanthrene (MCA) skin painting rapidly induced a 100% incidence of thymic lymphomas in RF/J mice. Tumors developed exclusively in cells of the T-lymphocyte lineage and displayed the surface phenotype: Thy-1+, Lyt-1+, Lyt-2+, Qa-1+, H-2K+, H-2D+, TL-, Ig-, Ia-. This phenotype resembled that of cortisone-resis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goodenow MM,Lilly F

更新日期：1984-12-01 00:00:00

abstract：:A discussion of the expected mechanism of radical reduction of carbonyl compounds by dihydroflavin as it relates to the structure of the carbonyl compound is provided. Factors which must be taken into account are the free energies of formation of the radical anions (CR2O), THE ACIDITY OF THE CONJUGATE ACID OF THE CARB...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williams RF,Shinkai S,Bruice TC

更新日期：1975-05-01 00:00:00

abstract：:In Escherichia coli, AK I- HDH I and AK II- HDH II are two bifunctional proteins, derived from a common ancestor, that catalyze the first and third reactions of the common pathway leading to threonine and methionine. An extensive amino acid sequence comparison of both molecules reveals two main features on each of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ferrara P,Duchange N,Zakin MM,Cohen GN

更新日期：1984-05-01 00:00:00

abstract：:The phosphatidylinositol 3-kinase subunit PIK3CA is frequently mutated in human cancers. Here we used gene targeting to "knock in" PIK3CA mutations into human breast epithelial cells to identify new therapeutic targets associated with oncogenic PIK3CA. Mutant PIK3CA knockin cells were capable of epidermal growth facto...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gustin JP,Karakas B,Weiss MB,Abukhdeir AM,Lauring J,Garay JP,Cosgrove D,Tamaki A,Konishi H,Konishi Y,Mohseni M,Wang G,Rosen DM,Denmeade SR,Higgins MJ,Vitolo MI,Bachman KE,Park BH

更新日期：2009-02-24 00:00:00

abstract：:Although introduced hemosporidian (malaria) parasites (Apicomplexa: Haemosporida) have hastened the extinction of endemic bird species in the Hawaiian Islands and perhaps elsewhere, little is known about the temporal dynamics of endemic malaria parasite populations. Haemosporidian parasites do not leave informative fo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Soares L,Latta SC,Ricklefs RE

更新日期：2017-06-20 00:00:00

abstract：:After the start of transcription, the 5' ends of eukaryotic mRNA molecules are modified by the addition of a guanylyl residue to form a cap structure, G(5')ppp(5')N. The guanylyltransferase (GTP:mRNA guanylyltransferase, EC 2.7.7.50) reaction responsible for cap formation usually proceeds via a covalent enzyme-GMP int...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ahola T,Kääriäinen L

更新日期：1995-01-17 00:00:00