Dynamics of avian haemosporidian assemblages through millennial time scales inferred from insular biotas of the West Indies.

abstract：:Gene delivery to the central nervous system is central to the development of gene therapy for neurological diseases. We developed a baculovirus-derived vector, the Bac-CMV-GFP vector, containing a reporter gene encoding for the green fluorescent protein (GFP) under the control of the cytomegalovirus (CMV) promoter. Tw...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sarkis C,Serguera C,Petres S,Buchet D,Ridet JL,Edelman L,Mallet J

更新日期：2000-12-19 00:00:00

abstract：:We describe a technique for transferring electrophoretically separated bands of RNA from an agarose gel to paper strips. The RNA is coupled covalently to diazobenzyloxymethyl groups on the paper. After transfer and appropriate treatment of the paper to destroy remaining diazo groups, specific RNA bands can be detected...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alwine JC,Kemp DJ,Stark GR

更新日期：1977-12-01 00:00:00

abstract：:A hallmark of most neurodegenerative diseases, including those caused by polyglutamine expansion, is the formation of ubiquitin (Ub)-positive protein aggregates in affected neurons. This finding suggests that the Ub system may be involved in common mechanisms underlying these otherwise unrelated diseases. Here we repo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Donaldson KM,Li W,Ching KA,Batalov S,Tsai CC,Joazeiro CA

更新日期：2003-07-22 00:00:00

abstract：:Mutant alleles at the dilute unconventional myosin heavy chain locus cause diluted coat color, opisthotonic seizures, and death. The dilute coat color phenotype is caused by irregular clumping of pigment in the hair, but amounts of melanin are unchanged from wild-type controls. The melanocyte phenotype has been descri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Provance DW Jr,Wei M,Ipe V,Mercer JA

更新日期：1996-12-10 00:00:00

abstract：:By using an indirect immunofluorescence technique, the distribution of poly(ADP-ribose) synthesis in human blood cells was investigated. The antibody used was reactive with poly(ADP-ribose) larger than trimers. The specific immunofluorescence of poly(ADP-ribose) synthesized in situ from NAD+ was observed in nuclei of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ikai K,Ueda K,Fukushima M,Nakamura T,Hayaishi O

更新日期：1980-06-01 00:00:00

abstract：:Aromatic polyketides make up a large class of natural products with diverse bioactivity. During biosynthesis, linear poly-β-ketone intermediates are regiospecifically cyclized, yielding molecules with defined cyclization patterns that are crucial for polyketide bioactivity. The aromatase/cyclases (ARO/CYCs) are respon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Caldara-Festin G,Jackson DR,Barajas JF,Valentic TR,Patel AB,Aguilar S,Nguyen M,Vo M,Khanna A,Sasaki E,Liu HW,Tsai SC

更新日期：2015-12-15 00:00:00

abstract：:Conformationally compromised oncogenic mutants of the tumor suppressor protein p53 can, in principle, be rescued by small molecules that bind the native, but not the denatured state. We describe a strategy for the rational search for such molecules. A nine-residue peptide, CDB3, which was derived from a p53 binding pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Friedler A,Hansson LO,Veprintsev DB,Freund SM,Rippin TM,Nikolova PV,Proctor MR,Rüdiger S,Fersht AR

更新日期：2002-01-22 00:00:00

abstract：:Plants, although sessile, can reorient growth axes in response to changing environmental conditions. Phototropism and gravitropism represent adaptive growth responses induced by changes in light direction and growth axis orientation relative to gravitational direction, respectively. The nearly 80-year-old Cholodny-Wen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Esmon CA,Tinsley AG,Ljung K,Sandberg G,Hearne LB,Liscum E

更新日期：2006-01-03 00:00:00

abstract：:To investigate protein folding dynamics in terms of compactness, we developed a continuous-flow mixing device to make small-angle x-ray scattering measurements with the time resolution of 160 micros and characterized the radius of gyration (R(g)) of two folding intermediates of cytochrome c (cyt c). The early intermed...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Akiyama S,Takahashi S,Kimura T,Ishimori K,Morishima I,Nishikawa Y,Fujisawa T

更新日期：2002-02-05 00:00:00

abstract：:Although ghrelin has been demonstrated to stimulate energy intake and storage through a central mechanism, its effect on hepatic lipid metabolism remains largely uncharacterized. Ghrelin receptor antagonism or gene deletion significantly decreased obesity-associated hepatic steatosis by suppression of de novo lipogene...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li Z,Xu G,Qin Y,Zhang C,Tang H,Yin Y,Xiang X,Li Y,Zhao J,Mulholland M,Zhang W

更新日期：2014-09-09 00:00:00

abstract：:There has been an increasing interest in cyanobacteria because these photosynthetic organisms convert solar energy into biomass and because of their potential for the production of biofuels. However, the exploitation of cyanobacteria for bioengineering requires knowledge of their transcriptional organization. Using di...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitschke J,Georg J,Scholz I,Sharma CM,Dienst D,Bantscheff J,Voss B,Steglich C,Wilde A,Vogel J,Hess WR

更新日期：2011-02-01 00:00:00

abstract：:The development of a spider silk-manufacturing process is of great interest. However, there are serious problems with natural manufacturing through spider farming, and standard recombinant protein production platforms have provided limited progress due to their inability to assemble spider silk proteins into fibers. T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Teulé F,Miao YG,Sohn BH,Kim YS,Hull JJ,Fraser MJ Jr,Lewis RV,Jarvis DL

更新日期：2012-01-17 00:00:00

abstract：:Artificially aminoacylated suppressor tRNAs were used to introduce photoreactive amino acids into model mitochondrial precursor proteins to probe the environment along the protein import pathway. Amino acids with benzophenone side chains of various lengths [DL-2-amino-3-(p-benzoylphenyl)propanoic acid (1) and DL-2-ami...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kanamori T,Nishikawa S,Shin I,Schultz PG,Endo T

更新日期：1997-01-21 00:00:00

abstract：:Epilepsy, a disease characterized by abnormal brain activity, is a disabling and potentially life-threatening condition for nearly 1% of the world population. Unfortunately, modulation of brain excitability using available antiepileptic drugs can have serious side effects, especially in the developing brain, and some ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baraban SC,Southwell DG,Estrada RC,Jones DL,Sebe JY,Alfaro-Cervello C,García-Verdugo JM,Rubenstein JL,Alvarez-Buylla A

更新日期：2009-09-08 00:00:00

abstract：:In response to environmental stresses, the mammalian serine threonine kinases PERK, GCN2, HRI, and PKR phosphorylate the regulatory serine 51 of the eukaryotic translation initiation factor 2α (eIF2α) to inhibit global protein synthesis. Plasmodium, the protozoan that causes malaria, expresses three eIF2α kinases: IK1...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang M,Mishra S,Sakthivel R,Rojas M,Ranjan R,Sullivan WJ Jr,Fontoura BM,Ménard R,Dever TE,Nussenzweig V

更新日期：2012-03-06 00:00:00

abstract：:Metazoan embryogenesis is controlled by a limited number of signaling modules that are used repetitively at successive developmental stages. The development of social amoebas shows similar reiterated use of cAMP-mediated signaling. In the model Dictyostelium discoideum, secreted cAMP acting on 4 cAMP receptors (cARs1-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kawabe Y,Morio T,James JL,Prescott AR,Tanaka Y,Schaap P

更新日期：2009-04-28 00:00:00

abstract：:Opportunistic infections are often polymicrobial. Two of the most important bacterial opportunistic pathogens of humans, Pseudomonas aeruginosa and Staphylococcus aureus, frequently are coisolated from infections of catheters, endotracheal tubes, skin, eyes, and the respiratory tract, including the airways of people w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoffman LR,Déziel E,D'Argenio DA,Lépine F,Emerson J,McNamara S,Gibson RL,Ramsey BW,Miller SI

更新日期：2006-12-26 00:00:00

abstract：:Although the applicability of small subunit ribosomal RNA (16S rRNA) sequences for bacterial classification is now well accepted, the general use of these molecules has been hindered by the technical difficulty of obtaining their sequences. A protocol is described for rapidly generating large blocks of 16S rRNA sequen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lane DJ,Pace B,Olsen GJ,Stahl DA,Sogin ML,Pace NR

更新日期：1985-10-01 00:00:00

abstract：:Protein homeostasis is critically important for cell viability. Key to this process is the refolding of misfolded or aggregated proteins by molecular chaperones or, alternatively, their degradation by proteases. In most prokaryotes and in chloroplasts and mitochondria, protein degradation is performed by the caseinoly...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vahidi S,Ripstein ZA,Bonomi M,Yuwen T,Mabanglo MF,Juravsky JB,Rizzolo K,Velyvis A,Houry WA,Vendruscolo M,Rubinstein JL,Kay LE

更新日期：2018-07-10 00:00:00

abstract：:Short oxygen-halogen interactions have been known in organic chemistry since the 1950s and recently have been exploited in the design of supramolecular assemblies. The present survey of protein and nucleic acid structures reveals similar halogen bonds as potentially stabilizing inter- and intramolecular interactions t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Auffinger P,Hays FA,Westhof E,Ho PS

更新日期：2004-11-30 00:00:00

abstract：:Cells of the craniofacial skeleton are derived from a common mesenchymal progenitor. The regulatory factors that control their differentiation into various cell lineages are unknown. To investigate the biological function of dentin matrix protein 1 (DMP1), an extracellular matrix gene involved in calcified tissue form...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Narayanan K,Srinivas R,Ramachandran A,Hao J,Quinn B,George A

更新日期：2001-04-10 00:00:00

abstract：:An approach based on mosquitoes carrying a conditional dominant lethal gene (release of insects carrying a dominant lethal, RIDL) is being developed to control the transmission of dengue viruses by vector population suppression. A transgenic strain, designated OX3604C, of the major dengue vector, Aedes aegypti, was en...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wise de Valdez MR,Nimmo D,Betz J,Gong HF,James AA,Alphey L,Black WC 4th

更新日期：2011-03-22 00:00:00

abstract：:Our understanding of the mechanisms of neural circuit assembly is far from complete. Identification of wiring molecules with novel mechanisms of action will provide insights into how complex and heterogeneous neural circuits assemble during development. In the Drosophila olfactory system, 50 classes of olfactory recep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xie Q,Wu B,Li J,Xu C,Li H,Luginbuhl DJ,Wang X,Ward A,Luo L

更新日期：2019-08-06 00:00:00

abstract：:There is no effective treatment for the cardiomyopathy of the most common autosomal recessive ataxia, Friedreich's ataxia (FA). The identification of potentially toxic mitochondrial (MIT) iron (Fe) deposits in FA suggests that Fe plays a role in its pathogenesis. This study used the muscle creatine kinase conditional ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Whitnall M,Suryo Rahmanto Y,Sutak R,Xu X,Becker EM,Mikhael MR,Ponka P,Richardson DR

更新日期：2008-07-15 00:00:00

abstract：:Molecular genetic analyses of the young pseudogenes of the immunoglobulin C epsilon genes were carried out to obtain qualitative evidence for the phylogenetic branching pattern of hominoid primates. We found that Old World monkeys had two C epsilon genes, one of which was processed. Among the hominoids examined only t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ueda S,Takenaka O,Honjo T

更新日期：1985-06-01 00:00:00

abstract：:Let X = Omega/Gamma be a compact quotient of an irreducible bounded symmetric domain Omega of rank >/=2 by a discrete group omega of automorphisms without fixed points. It is well known that the Kähler-Einstein metric g on X carries seminegative curvature (in the sense of Griffiths). I show that any Hermitian metric h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mok N

更新日期：1986-04-01 00:00:00

abstract：:We have used a microfluidic mass sensor to measure the density of single living cells. By weighing each cell in two fluids of different densities, our technique measures the single-cell mass, volume, and density of approximately 500 cells per hour with a density precision of 0.001 g mL(-1). We observe that the intrins...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grover WH,Bryan AK,Diez-Silva M,Suresh S,Higgins JM,Manalis SR

更新日期：2011-07-05 00:00:00

abstract：:Paired oxygen-isotopic analyses of abiotic carbonate and benthic-ostracode shells from lake sediments provide a continuous quantitative record of growing-season temperature for the past 2000 years in the northwestern foothills of the Alaska Range. This record reveals three time intervals of comparable warmth: anno Dom...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu FS,Ito E,Brown TA,Curry BB,Engstrom DR

更新日期：2001-09-11 00:00:00

abstract：:The synthesis of a photosensitive blocking group for the carboxyl function of neurotransmitters, in this case glycine, is reported. The compound, 2-methoxy-5-nitrophenyl glycine ester (caged glycine), is photolyzed by a laser pulse at 308 or 337 nm within 3 microseconds and with a product quantum yield of 0.2. The com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ramesh D,Wieboldt R,Niu L,Carpenter BK,Hess GP

更新日期：1993-12-01 00:00:00

abstract：:It is proved that the well known Nagata automorphism of the polynomial ring in three variables over a field of characteristic zero is wild, that is, it can not be decomposed into a product of elementary automorphisms. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shestakov IP,Umirbaev UU

更新日期：2003-10-28 00:00:00