Abstract:
:The development of a spider silk-manufacturing process is of great interest. However, there are serious problems with natural manufacturing through spider farming, and standard recombinant protein production platforms have provided limited progress due to their inability to assemble spider silk proteins into fibers. Thus, we used piggyBac vectors to create transgenic silkworms encoding chimeric silkworm/spider silk proteins. The silk fibers produced by these animals were composite materials that included chimeric silkworm/spider silk proteins integrated in an extremely stable manner. Furthermore, these composite fibers were, on average, tougher than the parental silkworm silk fibers and as tough as native dragline spider silk fibers. These results demonstrate that silkworms can be engineered to manufacture composite silk fibers containing stably integrated spider silk protein sequences, which significantly improve the overall mechanical properties of the parental silkworm silk fibers.
journal_name
Proc Natl Acad Sci U S Aauthors
Teulé F,Miao YG,Sohn BH,Kim YS,Hull JJ,Fraser MJ Jr,Lewis RV,Jarvis DLdoi
10.1073/pnas.1109420109subject
Has Abstractpub_date
2012-01-17 00:00:00pages
923-8issue
3eissn
0027-8424issn
1091-6490pii
1109420109journal_volume
109pub_type
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