The murMN operon: a functional link between antibiotic resistance and antibiotic tolerance in Streptococcuspneumoniae.

abstract：:Cytoplasmic RNA extracted from human tissue culture cells infected with adenovirus type 2 was used to program protein synthesis in a cell-free system derived from mammalian cells. Analysis of the protein product by polyacrylamide gel electrophoresis revealed ten adenovirus-specific polypeptides. Five of these were fur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anderson CW,Lewis JB,Atkins JF,Gesteland RF

更新日期：1974-07-01 00:00:00

abstract：:Mutations in the GABA(A) receptor gamma2 subunit are associated with childhood absence epilepsy and febrile seizures. To understand better the molecular basis of absence epilepsy in man, we developed a mouse model harboring a gamma2 subunit point mutation (R43Q) found in a large Australian family. Mice heterozygous fo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tan HO,Reid CA,Single FN,Davies PJ,Chiu C,Murphy S,Clarke AL,Dibbens L,Krestel H,Mulley JC,Jones MV,Seeburg PH,Sakmann B,Berkovic SF,Sprengel R,Petrou S

更新日期：2007-10-30 00:00:00

abstract：:We previously isolated a mutant of Escherichia coli that is preferentially affected in the synthesis of rRNA and has a mutation in the gene (accD) encoding a subunit of acetyl-CoA carboxylase. Using this mutant and other mutants of the pathway for fatty acid and phospholipid biosynthesis as well as cerulenin, a specif...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seyfzadeh M,Keener J,Nomura M

更新日期：1993-12-01 00:00:00

abstract：:Studies were undertaken to determine the applicability and effectiveness of immunoadsorption chromatography on anti-low density lipoprotein (LDL) columns as a plasma-cholesterol-lowering procedure. Mass production and isolation of monospecific antibodies against swine LDL (rho = 1.006-1.063 g/ml) was carried out by im...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stoffel W,Demant T

更新日期：1981-01-01 00:00:00

abstract：:The mitochondrial trifunctional protein (TFP) catalyzes three reactions in the fatty acid β-oxidation process. Mutations in the two TFP subunits cause mitochondrial trifunctional protein deficiency and acute fatty liver of pregnancy that can lead to death. Here we report a 4.2-Å cryo-electron microscopy α2β2 tetrameri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liang K,Li N,Wang X,Dai J,Liu P,Wang C,Chen XW,Gao N,Xiao J

更新日期：2018-07-03 00:00:00

abstract：:Efforts to delineate the advent of many enzymes essential to protein translation are often limited by the fact that the modern genetic code evolved before divergence of the tree of life. Glutaminyl-tRNA synthetase (GlnRS) is one noteworthy exception to the universality of the translation apparatus. In eukaryotes and s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Skouloubris S,Ribas de Pouplana L,De Reuse H,Hendrickson TL

更新日期：2003-09-30 00:00:00

abstract：:Human skin and lung mast cells and rodent peritoneal mast cells contain a carboxypeptidase in their secretory granules. We have screened human lung cDNA libraries with a mouse mast cell carboxypeptidase A (MC-CPA) cDNA probe to isolate a near-full-length cDNA that encodes human MC-CPA. The 5' end of the human MC-CPA t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reynolds DS,Gurley DS,Stevens RL,Sugarbaker DJ,Austen KF,Serafin WE

更新日期：1989-12-01 00:00:00

abstract：:Photo-triggered alpha-helix formation of a 16-residue peptide featuring a built-in conformational photoswitch is monitored by time-resolved IR spectroscopy. An experimental approach with 2-ps time resolution and a scanning range up to 30 micros is used to cover all time scales of the peptide dynamics. Experiments are ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bredenbeck J,Helbing J,Kumita JR,Woolley GA,Hamm P

更新日期：2005-02-15 00:00:00

abstract：:Previous research and theory suggest that two stable personality dimensions, extroversion and neuroticism, differentially influence emotional reactivity to a variety of pleasurable phenomena. Here, we use event-related functional MRI to address the putative neural and behavioral associations between humor appreciation...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mobbs D,Hagan CC,Azim E,Menon V,Reiss AL

更新日期：2005-11-08 00:00:00

abstract：:In the central nervous system, glycogen-derived bioenergetic resources in astrocytes help promote tissue survival in response to focal neuronal stress. However, our understanding of the extent to which these resources are mobilized and utilized during neurodegeneration, especially in nearby regions that are not active...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cooper ML,Pasini S,Lambert WS,D'Alessandro KB,Yao V,Risner ML,Calkins DJ

更新日期：2020-08-04 00:00:00

abstract：:Previously we reported that pretreatment of rats with the substance P (SP) antagonist CP-96,345 inhibits the enterotoxic responses following administration of toxin A from Clostridium difficile into ileal loops, indicating that SP participates in the intestinal responses to this toxin. We now report that injection of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Castagliuolo I,Keates AC,Qiu B,Kelly CP,Nikulasson S,Leeman SE,Pothoulakis C

更新日期：1997-04-29 00:00:00

abstract：:Thyrotoxicosis increases endogenous glucose production (EGP) and induces hepatic insulin resistance. We have recently shown that these alterations can be modulated by selective hepatic sympathetic and parasympathetic denervation, pointing to neurally mediated effects of thyroid hormone on glucose metabolism. Here, we ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Klieverik LP,Janssen SF,van Riel A,Foppen E,Bisschop PH,Serlie MJ,Boelen A,Ackermans MT,Sauerwein HP,Fliers E,Kalsbeek A

更新日期：2009-04-07 00:00:00

abstract：:In myocardial ischemia, adrenergic nerves release excessive amounts of norepinephrine (NE), causing dysfunction and arrhythmias. With anoxia and the concomitant ATP depletion, vesicular storage of NE is impaired, resulting in accumulation of free NE in the axoplasm of sympathetic nerves. Intraneuronal acidosis activat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Silver RB,Mackins CJ,Smith NC,Koritchneva IL,Lefkowitz K,Lovenberg TW,Levi R

更新日期：2001-02-27 00:00:00

abstract：:Like other guanine nucleotide-exchange proteins (GEPs) that activate ADP-ribosylation factor (ARF) GTPases, brefeldin A-inhibited GEP2, BIG2, contains an approximately 200-aa Sec7 domain that is responsible for this catalytic activity and its inhibition by brefeldin A. The Sec7 domain is located near the center of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li H,Adamik R,Pacheco-Rodriguez G,Moss J,Vaughan M

更新日期：2003-02-18 00:00:00

abstract：:The POU-IV or "Brn-3" class of POU-domain transcription factors is represented in Drosophila by I-POU and twin-of-I-POU, alternative splice products of the I-POU gene. I-POU has been previously reported to inhibit DNA binding by the POU-III class factor drifter/Cf1a via the formation of heterodimeric complexes. Here w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Turner EE

更新日期：1996-12-24 00:00:00

abstract：:Phosphatidylinositol 3,4,5-triphosphate (PIP3) plays a key role in neuronal polarization and axon formation. PIP3-containing vesicles are transported to axon tips by the kinesin KIF13B via an adaptor protein, centaurin α1 (CENTA1). KIF13B interacts with CENTA1 through its forkhead-associated (FHA) domain. We solved th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tong Y,Tempel W,Wang H,Yamada K,Shen L,Senisterra GA,MacKenzie F,Chishti AH,Park HW

更新日期：2010-11-23 00:00:00

abstract：:An examination of life expectancy in 1963 reveals twin peaks in the empirical distribution across countries: one group of countries clustered around a life expectancy of 40 years and a second group clustered around a life expectancy of 65 years. By 2003, the mode of each cluster had moved up by approximately 10 years....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bloom DE,Canning D

更新日期：2007-10-09 00:00:00

abstract：:A single dose of N-methyl-N-nitrosourea given to sexually immature female Buf/N rats produces a high incidence of mammary adenocarcinomas. A large percentage of these tumors contain the Ha-ras oncogene, activated by a G----A transition at the second nucleotide of codon 12. Copenhagen rats, on the other hand, are compl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu SJ,Archer MC

更新日期：1992-02-01 00:00:00

abstract：:The effect of RNA secondary structure on rho-independent and rho-dependent termination of transcription of T3 DNA by Escherichia coli RNA polymerase has been studied by incorporating, into nascent transcripts, base analogs that lead to altered base-pairing properties. A guanine --> hypoxanthine substitution, with atte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Adhya S,Sarkar P,Valenzuela D,Maitra U

更新日期：1979-04-01 00:00:00

abstract：:Two regioisomers with C3 or D3 symmetry of water-soluble carboxylic acid C60 derivatives, containing three malonic acid groups per molecule, were synthesized and found to be equipotent free radical scavengers in solution as assessed by EPR analysis. Both compounds also inhibited the excitotoxic death of cultured corti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dugan LL,Turetsky DM,Du C,Lobner D,Wheeler M,Almli CR,Shen CK,Luh TY,Choi DW,Lin TS

更新日期：1997-08-19 00:00:00

abstract：:Ubiquitin C-terminal hydrolase L1 (UCH-L1) is one of the most abundant and enigmatic enzymes of the CNS. Based on existing UCH-L1 knockout models, UCH-L1 is thought to be required for the maintenance of axonal integrity, but not for neuronal development despite its high expression in neurons. Several lines of evidence...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reinicke AT,Laban K,Sachs M,Kraus V,Walden M,Damme M,Sachs W,Reichelt J,Schweizer M,Janiesch PC,Duncan KE,Saftig P,Rinschen MM,Morellini F,Meyer-Schwesinger C

更新日期：2019-04-16 00:00:00

abstract：:RS67333 is a partial serotonin subtype 4 receptor (5-HT4R) agonist that has been widely studied for its procognitive effect. More recently, it has been shown that its ability to promote the nonamyloidogenic cleavage of the precursor of the neurotoxic amyloid-β peptide leads to the secretion of the neurotrophic protein...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lecoutey C,Hedou D,Freret T,Giannoni P,Gaven F,Since M,Bouet V,Ballandonne C,Corvaisier S,Malzert Fréon A,Mignani S,Cresteil T,Boulouard M,Claeysen S,Rochais C,Dallemagne P

更新日期：2014-09-09 00:00:00

abstract：:Classical studies suggest that high-level cognitive decisions (e.g., choosing between financial options) are suboptimal. In contrast, low-level decisions (e.g., choosing where to put your feet on a rocky ridge) appear near-optimal: the perception-cognition gap. Moreover, in classical tasks, people appear to put too mu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jarvstad A,Hahn U,Rushton SK,Warren PA

更新日期：2013-10-01 00:00:00

abstract：:Ion channels in excitable cells function in macromolecular complexes in which auxiliary proteins modulate the biophysical properties of the pore-forming subunits. Hyperpolarization-activated, cyclic nucleotide-sensitive HCN4 channels are critical determinants of membrane excitability in cells throughout the body, incl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peters CH,Myers ME,Juchno J,Haimbaugh C,Bichraoui H,Du Y,Bankston JR,Walker LA,Proenza C

更新日期：2020-07-28 00:00:00

abstract：:Tumor-infiltrating lymphocytes (TIL) obtained from human melanomas can specifically lyse autologous tumor in vitro and mediate tumor regression in vivo. To develop more effective therapeutic reagents and to further understand the T-cell response to tumors, the diversity of T-cell receptors (TCRs) involved in melanoma ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shilyansky J,Nishimura MI,Yannelli JR,Kawakami Y,Jacknin LS,Charmley P,Rosenberg SA

更新日期：1994-03-29 00:00:00

abstract：:One half of a group of 20 patients with human papillomavirus type 16 (HPV16)-induced vulvar intraepithelial neoplasia grade 3 displayed a complete regression (CR) after therapeutic vaccination with HPV16 E6/E7 synthetic long peptides. Patients with relatively larger lesions generally did not display a CR. To investiga...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Welters MJ,Kenter GG,de Vos van Steenwijk PJ,Löwik MJ,Berends-van der Meer DM,Essahsah F,Stynenbosch LF,Vloon AP,Ramwadhdoebe TH,Piersma SJ,van der Hulst JM,Valentijn AR,Fathers LM,Drijfhout JW,Franken KL,Oostendorp J,Fle

更新日期：2010-06-29 00:00:00

abstract：:Modern protein crystal structures are based nearly exclusively on X-ray data collected at cryogenic temperatures (generally 100 K). The cooling process is thought to introduce little bias in the functional interpretation of structural results, because cryogenic temperatures minimally perturb the overall protein backbo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fraser JS,van den Bedem H,Samelson AJ,Lang PT,Holton JM,Echols N,Alber T

更新日期：2011-09-27 00:00:00

abstract：:Most B lymphocytes in normal individuals express two classes of cell-surface immunoglobulins, IgM and IgD. The specificity of the two antigen receptors is identical since they are produced by transcription and differential splicing of the same variable region gene segment to the heavy-chain constant region gene segmen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Irsch J,Irlenbusch S,Radl J,Burrows PD,Cooper MD,Radbruch AH

更新日期：1994-02-15 00:00:00

abstract：:The 369-residue glycoprotein D (gD) is the entry, receptor-binding protein of herpes simplex virus 1. The common receptors for viral entry are nectin-1, HveA, and a specific O-linked sulfated proteoglycan. The major receptor-binding sites of gD are at the N terminus, whereas the domain required for fusion of viral env...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhou G,Roizman B

更新日期：2007-03-06 00:00:00

abstract：:We have previously identified a generally occurring short-range mobility of nucleosome cores on DNA in relatively low ionic strength conditions. Here we report that this mobility of histone octamers positioned on constructs of 5S rDNA is suppressed by the binding of histone H1 or H5 to the nucleosome. Histone H5 is th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pennings S,Meersseman G,Bradbury EM

更新日期：1994-10-25 00:00:00