Accessing protein conformational ensembles using room-temperature X-ray crystallography.

abstract：:Recent observations suggest that p53 mutations are responsible not only for growth of primary tumors but also for their dissemination. However, mechanisms involved in p53-mediated control of cell motility and invasion remain poorly understood. By using the primary ovarian surface epithelium cell culture, we show that ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hwang CI,Matoso A,Corney DC,Flesken-Nikitin A,Körner S,Wang W,Boccaccio C,Thorgeirsson SS,Comoglio PM,Hermeking H,Nikitin AY

更新日期：2011-08-23 00:00:00

abstract：:Acquired resistance to EGF receptor (EGFR) tyrosine kinase inhibitors (TKIs) is inevitable in metastatic EGFR-mutant lung cancers. Here, we modeled disease progression using EGFR-mutant human tumor cell lines. Although five of six models displayed alterations already found in humans, one harbored an unexpected seconda...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ohashi K,Sequist LV,Arcila ME,Moran T,Chmielecki J,Lin YL,Pan Y,Wang L,de Stanchina E,Shien K,Aoe K,Toyooka S,Kiura K,Fernandez-Cuesta L,Fidias P,Yang JC,Miller VA,Riely GJ,Kris MG,Engelman JA,Vnencak-Jones CL,D

更新日期：2012-07-31 00:00:00

abstract：:The 23S twisted circular form of ColE(1) DNA has been isolated from Escherichia coli as a tightly associated DNA-protein complex with a sedimentation coefficient of approximately 24S. Treatment of this complex with pronase, trypsin, sodium dodecyl sulfate, Sarkosyl, or heat results in a conversion to a slower sediment...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clewell DB,Helinski DR

更新日期：1969-04-01 00:00:00

abstract：:Previous studies have shown that the immunosuppressive and carcinogenic polycyclic aromatic hydrocarbon 7,12-dimethylbenz(a)anthracene (DMBA) impairs Ca(2+)-dependent transmembrane signaling in human and murine lymphocytes. The purpose of the present studies was to analyze potential mechanisms of immunosuppression by ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Archuleta MM,Schieven GL,Ledbetter JA,Deanin GG,Burchiel SW

更新日期：1993-07-01 00:00:00

abstract：:Rapamycin is an immunosuppressive immunophilin ligand reported as having neurotrophic activity. We show that modification of rapamycin at the mammalian target of rapamycin (mTOR) binding region yields immunophilin ligands, WYE-592 and ILS-920, with potent neurotrophic activities in cortical neuronal cultures, efficacy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ruan B,Pong K,Jow F,Bowlby M,Crozier RA,Liu D,Liang S,Chen Y,Mercado ML,Feng X,Bennett F,von Schack D,McDonald L,Zaleska MM,Wood A,Reinhart PH,Magolda RL,Skotnicki J,Pangalos MN,Koehn FE,Carter GT,Abou-Gharbia M

更新日期：2008-01-08 00:00:00

abstract：:According to a current "RNA first" model for the origin of life, RNA emerged in some form on early Earth to become the first biopolymer to support Darwinism here. Threose nucleic acid (TNA) and other polyelectrolytes are also considered as the possible first Darwinian biopolymer(s). This model is being developed by re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim HJ,Benner SA

更新日期：2017-10-24 00:00:00

abstract：:Long-range conformational changes in proteins are ubiquitous in biology for the transmission and amplification of signals; such conformational changes can be triggered by small-amplitude, nanosecond protein domain motion. Understanding how conformational changes are initiated requires the characterization of protein d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bu Z,Biehl R,Monkenbusch M,Richter D,Callaway DJ

更新日期：2005-12-06 00:00:00

abstract：:The plant viruses alfalfa mosaic virus (AMV) and brome mosaic virus (BMV) each divide their genetic information among three RNAs while tobacco mosaic virus (TMV) contains a single genomic RNA. Amino acid sequence comparisons suggest that the single proteins encoded by AMV RNA 1 and BMV RNA 1 and by AMV RNA 2 and BMV R...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haseloff J,Goelet P,Zimmern D,Ahlquist P,Dasgupta R,Kaesberg P

更新日期：1984-07-01 00:00:00

abstract：:Presynaptic and postsynaptic differentiation occurs at axodendritic contacts between CNS neurons. Synaptic adhesion mediated by synaptic cell adhesion molecule (SynCAM) and beta-neurexins/neuroligins triggers presynaptic differentiation. The signals that trigger postsynaptic differentiation are, however, unknown. Here...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nam CI,Chen L

更新日期：2005-04-26 00:00:00

abstract：:The self-adjoint algebra of operators generated by the semigroup of translation operators acting on the Hilbert space of functions supported on the half-line is studied. A real-valued index is introduced and is used to determine the spectrum of the Wiener-Hopf integral operators with distribution kernel having an almo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coburn LA,Douglas RG

更新日期：1969-04-01 00:00:00

abstract：:Continuous and intensive exploitation of wildlife resources by early agricultural societies had major ecological consequences in the ancient Near East. In particular, hunting strategies of post-Neolithic societies involving the mass killing of wild ungulates contributed to the eventual extirpation of a number of wild ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bar-Oz G,Zeder M,Hole F

更新日期：2011-05-03 00:00:00

abstract：:The vacuolar (H(+))-ATPases (V-ATPases) are ATP-dependent proton pumps that acidify intracellular compartments and pump protons across specialized plasma membranes. Proton translocation occurs through the integral V(0) domain, which contains five different subunits (a, d, c, c', and c"). Proton transport is critically...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kawasaki-Nishi S,Nishi T,Forgac M

更新日期：2001-10-23 00:00:00

abstract：:Bruton tyrosine kinase (EC 2.7.1.112) [Btk, encoded by Btk in mice and BTK in humans (formerly known as atk, BPK, or emb)], which is variously mutated in chromosome X-linked agammaglobulinemia patients and X-linked immunodeficient (xid) mice, has the pleckstrin homology (PH) domain at its amino terminus. The PH domain...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yao L,Kawakami Y,Kawakami T

更新日期：1994-09-13 00:00:00

abstract：:There is considerable evidence that links the activation of cellular genes to oncogenesis. We previously reported that structural rearrangements in the cellular oncogene c-erbB correlate with the development of erythroblastosis induced by avian leukosis virus (ALV). c-erbB recently has been shown to be related to the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raines MA,Lewis WG,Crittenden LB,Kung HJ

更新日期：1985-04-01 00:00:00

abstract：:Genome engineering in human pluripotent stem cells (hPSCs) holds great promise for biomedical research and regenerative medicine. Recently, an RNA-guided, DNA-cleaving interference pathway from bacteria [the type II clustered, regularly interspaced, short palindromic repeats (CRISPR)-CRISPR-associated (Cas) pathway] h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hou Z,Zhang Y,Propson NE,Howden SE,Chu LF,Sontheimer EJ,Thomson JA

更新日期：2013-09-24 00:00:00

abstract：:Several lines of evidence suggest that the serotonin (5-hydroxytryptamine, 5-HT) regulates cardiovascular functions during embryogenesis and adulthood. 5-HT binds to numerous cognate receptors to initiate its biological effects. However, none of the 5-HT receptor disruptions in mice have yet resulted in embryonic defe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nebigil CG,Choi DS,Dierich A,Hickel P,Le Meur M,Messaddeq N,Launay JM,Maroteaux L

更新日期：2000-08-15 00:00:00

abstract：:Loss of the tumor suppressor, PTEN, is one of the most common findings in prostate cancer (PCa). This loss leads to overactive Akt signaling, which is correlated with increased metastasis and androgen independence. However, another tumor suppressor, inositol-polyphosphate 4-phosphatase type II (INPP4B), can partially ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chaurasiya S,Wu W,Strom AM,Warner M,Gustafsson JÅ

更新日期：2020-10-20 00:00:00

abstract：:Plasminogen activator inhibitor-1 (PAI-1) plays an important role in the pathogenesis of obesity-driven type 2 diabetes mellitus and associated cardiovascular complications. Here, we show that perturbation of caveolar microdomains leads to insulin resistance and concomitant up-regulation of PAI-1 in 3T3L1 adipocytes. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Venugopal J,Hanashiro K,Yang ZZ,Nagamine Y

更新日期：2004-12-07 00:00:00

abstract：:Developmental hemoglobin switching involves sequential globin gene activations and repressions that are incompletely understood. Earlier observations, described herein, led us to hypothesize that nuclear ferritin is a repressor of the adult beta-globin gene in embryonic erythroid cells. Our data show that a ferritin-f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Broyles RH,Belegu V,DeWitt CR,Shah SN,Stewart CA,Pye QN,Floyd RA

更新日期：2001-07-31 00:00:00

abstract：:To study C(0)a priori estimates for solutions to certain complex Monge-Ampère equations, I introduce a coherent sheaf of ideals and show that it satisfies various global algebrogeometric conditions, including a cohomology vanishing theorem. This technique is used to establish the existence of Kähler-Einstein metrics o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nadel AM

更新日期：1989-10-01 00:00:00

abstract：:In Drosophila, multiple isoforms of alpha-glycerol-3-phosphate dehydrogenase (sn-glycerol-3-phosphate: NAD+ 2-oxidoreductase, EC 1.1.1.8) are produced in a tissue- and stage-specific manner. To understand the underlying molecular basis of these isoforms, we have sequenced a 5.8-kilobase region of the Drosophila genome...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： von Kalm L,Weaver J,DeMarco J,MacIntyre RJ,Sullivan DT

更新日期：1989-07-01 00:00:00

abstract：:Glycogenin initiates the synthesis of a maltosaccharide chain covalently attached to itself on Tyr195 via a stepwise glucosylation reaction, priming glycogen synthesis. We have captured crystallographic snapshots of human glycogenin during its reaction cycle, revealing a dynamic conformational switch between ground an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chaikuad A,Froese DS,Berridge G,von Delft F,Oppermann U,Yue WW

更新日期：2011-12-27 00:00:00

abstract：:Monoclonal antibodies provide an attractive alternative to small-molecule therapies for a wide range of diseases. Given the importance of G protein-coupled receptors (GPCRs) as pharmaceutical targets, there has been an immense interest in developing therapeutic monoclonal antibodies that act on GPCRs. Here we present ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishchenko A,Wacker D,Kapoor M,Zhang A,Han GW,Basu S,Patel N,Messerschmidt M,Weierstall U,Liu W,Katritch V,Roth BL,Stevens RC,Cherezov V

更新日期：2017-08-01 00:00:00

abstract：:Molecular imaging probes have potential for in vivo identification of apoptosis and other intracellular processes. TcapQ, a cell-penetrating, near-infrared fluorescent peptide probe designed to be optically silent through intramolecular fluorescence quenching and activated by effector caspases, has been previously des...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barnett EM,Zhang X,Maxwell D,Chang Q,Piwnica-Worms D

更新日期：2009-06-09 00:00:00

abstract：:Leukocyte adherence to endothelium is in part mediated by the transient expression of endothelial-leukocyte adhesion molecule 1 (ELAM-1) on endothelial surfaces stimulated by tumor necrosis factor alpha (TNF), interleukin (IL) 1, or bacterial lipopolysaccharide (LPS). The intracellular factors controlling induction of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Montgomery KF,Osborn L,Hession C,Tizard R,Goff D,Vassallo C,Tarr PI,Bomsztyk K,Lobb R,Harlan JM

更新日期：1991-08-01 00:00:00

abstract：:Defined by monoclonal antibody GA733, the GA733-2 antigen is a cell surface 40-kDa glycoprotein associated with human carcinomas of various origins. Molecular clones for the GA733-2 antigen were isolated from a colorectal carcinoma cell line cDNA library using the high-efficiency COS cell expression system. A 1.4-kilo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Szala S,Froehlich M,Scollon M,Kasai Y,Steplewski Z,Koprowski H,Linnenbach AJ

更新日期：1990-05-01 00:00:00

abstract：:Rett syndrome is a severe neurodevelopmental disorder caused by mutations in the methyl-CpG binding protein gene (MECP2). MeCP2 is a methyl-cytosine binding protein that is proposed to function as a transcriptional repressor. However, multiple gene expression studies comparing wild-type and MeCP2-deficient neurons hav...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kinde B,Wu DY,Greenberg ME,Gabel HW

更新日期：2016-12-27 00:00:00

abstract：:In extracts of interferon-treated HeLa cells, RNA covalently linked to double-stranded RNA (dsRNA) is preferentially degraded compared with mRNA not linked in dsRNA. This was established by following the degradation of poly(A)-containing mRNA annealed with poly(U), of poly(C)-containing encephalomyocarditis virus RNA ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nilsen TW,Baglioni C

更新日期：1979-06-01 00:00:00

abstract：:Scientific cinematography using ultrafast optical imaging is a common tool to study motion. In opaque organisms or structures, X-ray radiography captures sequences of 2D projections to visualize morphological dynamics, but for many applications full four-dimensional (4D) spatiotemporal information is highly desirable....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： dos Santos Rolo T,Ershov A,van de Kamp T,Baumbach T

更新日期：2014-03-18 00:00:00

abstract：:Although mathematical models and laboratory experiments have shown that species interactions can generate chaos, field evidence of chaos in natural ecosystems is rare. We report on a pristine rocky intertidal community located in one of the world's oldest marine reserves that has displayed a complex cyclic succession ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benincà E,Ballantine B,Ellner SP,Huisman J

更新日期：2015-05-19 00:00:00