A noncognate aminoacyl-tRNA synthetase that may resolve a missing link in protein evolution.

abstract：:Pyrimethamine (Pyr) targets dihydrofolate reductase of Plasmodium vivax (PvDHFR) as well as other malarial parasites, but its use as antimalarial is hampered by the widespread high resistance. Comparison of the crystal structures of PvDHFR from wild-type and the Pyr-resistant (SP21, Ser-58 --> Arg + Ser-117 --> Asn) s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kongsaeree P,Khongsuk P,Leartsakulpanich U,Chitnumsub P,Tarnchompoo B,Walkinshaw MD,Yuthavong Y

更新日期：2005-09-13 00:00:00

abstract：:The response of the nematic twist-bend ([Formula: see text]) phase to an applied field can provide important insight into the structure of this liquid and may bring us closer to understanding mechanisms generating mirror symmetry breaking in a fluid of achiral molecules. Here we investigate theoretically how an extern...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pająk G,Longa L,Chrzanowska A

更新日期：2018-10-30 00:00:00

abstract：:It was shown in an earlier paper how to connect, in principle, the biochemical states of a cross-bridge with the mechanics of muscular contraction, by the methods of statistical mechanics. The treatment applies to cross-bridges that are able to interact with only one actin site at a time. The present paper shows that ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hill TL

更新日期：1973-10-01 00:00:00

abstract：:Coherent anti-Stokes Raman scattering (CARS) spectra are reported for flavin adenine dinucleotide (FAD) and glucose oxidase (beta-D-glucose:oxygen 1-oxidoreductase; EC 1.1.3.4), in resonance with the 450 nm flavin absorption band. Several isoalloxazine ring modes were observed (1635, 1584, 1507, 1416, and 1359 cm(-1))...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dutta PK,Nestor JR,Spiro TG

更新日期：1977-10-01 00:00:00

abstract：:Transient Receptor Potential Melastatin-8 (TRPM8), a recently identified member of the transient receptor potential (TRP) family of ion channels, is activated by mild cooling and by chemical compounds such as the supercooling agent, icilin. Since cooling, possibly involving TRPM8 stimulation, diminishes injury-induced...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ramachandran R,Hyun E,Zhao L,Lapointe TK,Chapman K,Hirota CL,Ghosh S,McKemy DD,Vergnolle N,Beck PL,Altier C,Hollenberg MD

更新日期：2013-04-30 00:00:00

abstract：:Most of our knowledge about transmission at central synapses has been obtained by studying populations of synapses, but some important properties of synapses can be determined only by studying them individually. An important issue is whether a presynaptic action potential causes, at most, a single vesicle to be releas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Conti R,Lisman J

更新日期：2003-04-15 00:00:00

abstract：:Phosphorylation is a major mechanism regulating the activity of ion channels that remains poorly understood with respect to T-type calcium channels (Cav3). These channels are low voltage-activated calcium channels that play a key role in cellular excitability and various physiological functions. Their dysfunction has ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Blesneac I,Chemin J,Bidaud I,Huc-Brandt S,Vandermoere F,Lory P

更新日期：2015-11-03 00:00:00

abstract：:Certain cheU mutants of Salmonella show inverted chemotactic behavior, being repelled by attractants and attracted by repellents. Such a dramatic change in behavioral pattern would seem at first glance to require drastic and complex alterations in the sensory processing system. In fact, the behavior can be explained b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Khan S,Macnab RM,DeFranco AL,Koshland DE Jr

更新日期：1978-09-01 00:00:00

abstract：:The Get3 ATPase directs the delivery of tail-anchored (TA) proteins to the endoplasmic reticulum (ER). TA-proteins are characterized by having a single transmembrane helix (TM) at their extreme C terminus and include many essential proteins, such as SNAREs, apoptosis factors, and protein translocation components. Thes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Suloway CJ,Chartron JW,Zaslaver M,Clemons WM Jr

更新日期：2009-09-01 00:00:00

abstract：:Although preterm delivery is a major global health issue, its causes and underlying mechanism remain elusive. Using mutant mice, mimicking aspects of human preterm birth, we show here that uterine decidual senescence early in pregnancy via heightened mammalian target of rapamycin complex 1 (mTORC1) signaling is a sign...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hirota Y,Cha J,Yoshie M,Daikoku T,Dey SK

更新日期：2011-11-01 00:00:00

abstract：:The Sleeping Beauty (SB) transposon system can integrate foreign sequences of DNA in the genome of mouse somatic cells eliciting long-term expression in vivo. This technology holds great promise for human gene therapy as a nonviral technology to deliver therapeutic genes. SB also provides a means to study the effects ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carlson CM,Frandsen JL,Kirchhof N,McIvor RS,Largaespada DA

更新日期：2005-11-22 00:00:00

abstract：:We combine direct surface force measurements with thermodynamic arguments to demonstrate that pure ionic liquids are expected to behave as dilute weak electrolyte solutions, with typical effective dissociated ion concentrations of less than 0.1% at room temperature. We performed equilibrium force-distance measurements...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gebbie MA,Valtiner M,Banquy X,Fox ET,Henderson WA,Israelachvili JN

更新日期：2013-06-11 00:00:00

abstract：:Cysteine dioxygenase (CDO) catalyzes the oxidation of l-cysteine to cysteine sulfinic acid. Deficiencies in this enzyme have been linked to autoimmune diseases and neurological disorders. The x-ray crystal structure of CDO from Mus musculus was solved to a nominal resolution of 1.75 Angstroms. The sequence is 91% iden...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McCoy JG,Bailey LJ,Bitto E,Bingman CA,Aceti DJ,Fox BG,Phillips GN Jr

更新日期：2006-02-28 00:00:00

abstract：:Commensal and pathogenic bacteria must deal with many different stress conditions to survive in and colonize the human gastrointestinal tract. One major challenge that bacteria encounter in the gut is the high concentration of bile salts, which not only aid in food absorption but also act as effective physiological an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cremers CM,Knoefler D,Vitvitsky V,Banerjee R,Jakob U

更新日期：2014-04-22 00:00:00

abstract：:We report here that component members of the keratin intermediate filament (IF) type I and type II gene families of sheep are closely linked but apparently the two families are not. Nine genes, accounting for up to half of the keratin IF gene repertoire, were mapped in four cosmid clones and the linkage between the ge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Powell BC,Cam GR,Fietz MJ,Rogers GE

更新日期：1986-07-01 00:00:00

abstract：:The toxic, mutagenic, and carcinogenic effects of alkylating agents have been attributed to their ability to damage DNA. Reaction at the O6 position of guanine results in miscoding during DNA replication, has been shown to be mutagenic in both bacteriophage and bacteria, and may be responsible for malignant transforma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brennand J,Margison GP

更新日期：1986-09-01 00:00:00

abstract：:We tested the influence of a photothrombotic lesion in somatosensory cortex on plasticity in the mouse visual system and the efficacy of anti-inflammatory treatment to rescue compromised learning. To challenge plasticity mechanisms, we induced monocular deprivation (MD) in 3-mo-old mice. In control animals, MD induced...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Greifzu F,Schmidt S,Schmidt KF,Kreikemeier K,Witte OW,Löwel S

更新日期：2011-09-13 00:00:00

abstract：:A key challenge in ecology is to define species' niches on the basis of functional traits. Size and shape are important determinants of a species' niche but their causal role is often difficult to interpret. For endotherms, size and shape define the thermal niche through their interaction with core temperature, insula...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Porter WP,Kearney M

更新日期：2009-11-17 00:00:00

abstract：:A predictive coarse-grained protein force field [associative memory, water-mediated, structure, and energy model for molecular dynamics (AWSEM)-MD] is used to study the energy landscapes and relative stabilities of amyloid-β protein (1-40) in the monomer and all of its oligomeric forms up to an octamer. We find that a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zheng W,Tsai MY,Chen M,Wolynes PG

更新日期：2016-10-18 00:00:00

abstract：:The combination of lutropin (LH; luteinizing hormone) alpha and beta subunits was examined in rat pituitaries incubated with [35S]methionine or [35S]sulfate. Combination was assessed by using antiserum directed against the beta subunit. The data show that combination of most of the subunits proceeds rapidly, well befo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoshina H,Boime I

更新日期：1982-12-01 00:00:00

abstract：:Genetic switches based on the [Formula: see text] system are master regulators of an array of cellular responses. Recent kinetic experiments have shown that [Formula: see text] can actively remove NF-κB bound to its genetic sites via a process called "molecular stripping." This allows the [Formula: see text] switch to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Potoyan DA,Zheng W,Komives EA,Wolynes PG

更新日期：2016-01-05 00:00:00

abstract：:Swarming and mass migration are spectacular and sometimes devastating features of the biology of various animal species. These phenomena are typically associated with actual or anticipated depletion of food resources after an increase in population density, but the mechanisms driving such collective movements are poor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Simpson SJ,Sword GA,Lorch PD,Couzin ID

更新日期：2006-03-14 00:00:00

abstract：:Amyloid beta-protein (Abeta) is linked to neuronal injury and death in Alzheimer's disease (AD). Of particular relevance for elucidating the role of Abeta in AD is new evidence that oligomeric forms of Abeta are potent neurotoxins that play a major role in neurodegeneration and the strong association of the 42-residue...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bitan G,Kirkitadze MD,Lomakin A,Vollers SS,Benedek GB,Teplow DB

更新日期：2003-01-07 00:00:00

abstract：:Small molecules that specifically bind with high affinity to any designated DNA sequence in the human genome would be useful tools in molecular biology and potentially in human medicine. Simple rules have been developed to rationally alter the sequence specificity of minor groove-binding polyamides containing N-methyl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cho J,Parks ME,Dervan PB

更新日期：1995-10-24 00:00:00

abstract：:The rate of Cl- secretion by human airway epithelium is determined, in part, by apical cell membrane Cl- conductance. In cystic fibrosis airway epithelia, defective regulation of Cl- conductance decreases the capability to secrete Cl-. Here we report that extracytosolic ATP in the luminal bath of cultured human airway...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stutts MJ,Chinet TC,Mason SJ,Fullton JM,Clarke LL,Boucher RC

更新日期：1992-03-01 00:00:00

abstract：:Most paleo-episodes of ocean acidification (OA) were either too slow or too small to be instructive in predicting near-future impacts. The end-Cretaceous event (66 Mya) is intriguing in this regard, both because of its rapid onset and also because many pelagic calcifying species (including 100% of ammonites and more t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tyrrell T,Merico A,Armstrong McKay DI

更新日期：2015-05-26 00:00:00

abstract：:The detailed molecular dynamics of an actual bond-breaking event in a fluctuating enzyme substrate complex is simulated. The method developed allows one to explore what type of fluctuations are involved in enzymatic reactions and to evaluate entropic contributions to enzyme catalysis. The fluctuations of the enzyme el...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Warshel A

更新日期：1984-01-01 00:00:00

abstract：:Ultraviolet photosensitivity of the single-stranded forms of poly(A) and poly(dA) is compared. In contrast to poly(A), poly(dA) is rather sensitive to ultraviolet irradiation. Relatively low doses of ultraviolet light lead to characteristic changes of the ultraviolet and circular dichroism spectra of poly(dA), suggest...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pörschke D

更新日期：1973-09-01 00:00:00

abstract：:A type of heritable cellular change, known as habituation, occurs spontaneously in plant tissue and cell culture. This phenomenon is characterized by a newly acquired capacity of plant cells to produce growth regulatory substances. Using cloned lines of tobacco pith parenchyma cells, we demonstrated that a newly acqui...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Binns A,Meins F

更新日期：1973-09-01 00:00:00

abstract：:Despite nearly four decades of effort, broad inhibition of oncogenic RAS using small-molecule approaches has proven to be a major challenge. Here we describe the development of a pan-RAS biologic inhibitor composed of the RAS-RAP1-specific endopeptidase fused to the protein delivery machinery of diphtheria toxin. We s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vidimar V,Beilhartz GL,Park M,Biancucci M,Kieffer MB,Gius DR,Melnyk RA,Satchell KJF

更新日期：2020-07-21 00:00:00