Resonance coherent anti-Stokes Raman scattering spectra of fluorescent biological chromophores: Vibrational evidence for hydrogen bonding of flavin to glucose oxidase and for rapid solvent exchange.

abstract：:Yields of beta-hydroxynitrates, dihydroxynitrates, and trihydroxynitrates, in particles formed from OH radical-initiated reactions of C(9)-C(15) 2-methyl-1-alkenes in the presence of NO(x) were measured by using a thermal desorption particle beam mass spectrometer coupled to a high-performance liquid chromatograph wit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matsunaga A,Ziemann PJ

更新日期：2010-04-13 00:00:00

abstract：:Antisense RNA to the 27/32-kDa rat liver gap junction (GJ) protein was used to explore the role of GJs in preimplantation embryos. When all blastomeres of two- and four-cell embryos were injected with GJ antisense RNA, the percentage of embryos compacted at 60 hr of development was reduced to less than 20%, while 90% ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bevilacqua A,Loch-Caruso R,Erickson RP

更新日期：1989-07-01 00:00:00

abstract：:We report a method for introducing mtDNA mutations into the mouse female germ line by means of embryonic stem (ES) cell cybrids. Mitochondria were recovered from the brain of a NZB mouse by fusion of synaptosomes to a mtDNA-deficient (rho degrees ) cell line. These cybrids were enucleated and the cytoplasts were elect...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sligh JE,Levy SE,Waymire KG,Allard P,Dillehay DL,Nusinowitz S,Heckenlively JR,MacGregor GR,Wallace DC

更新日期：2000-12-19 00:00:00

abstract：:Much debate has revolved around the question of whether the mode of evolutionary and ecological turnover in the fossil record of African mammals was continuous or pulsed, and the degree to which faunal turnover tracked changes in global climate. Here, we assembled and analyzed large specimen databases of the fossil re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bibi F,Kiessling W

更新日期：2015-08-25 00:00:00

abstract：:The cellular response to ionizing radiation (IR) includes induction of the c-jun and EGR1 early response genes. The present work has examined potential cytoplasmic signaling cascades that transduce IR-induced signals to the nucleus. The results demonstrate activation of the 40S ribosomal protein S6 kinase, pp90rsk, in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kharbanda S,Saleem A,Shafman T,Emoto Y,Weichselbaum R,Kufe D

更新日期：1994-06-07 00:00:00

abstract：:The effects of pyrophosphate on RNA binding and ATPase activities of Escherichia coli transcription termination factor rho have been studied. Mutant rho-115 protein has a temperature-sensitive RNA-dependent ATPase activity due to the thermolability of binding to RNA [Kent, R.B. & Guterman, S.K. (1981) Fed. Proc. Fed. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kent RB,Guterman SK

更新日期：1982-07-01 00:00:00

abstract：:The pH gradient (delta pH) across the membrane of bovine neurohypophysial granules was estimated by using the [14C]methylamine partition technique. The granule membrane showed high permeability to sugars such as sucrose or sorbitol and to cations. Granules suspended in sucrose medium showed an acidic internal delta pH...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scherman D,Nordmann JJ

更新日期：1982-01-01 00:00:00

abstract：:A rice Dwarf 1 gene was identified by using a map-based cloning strategy. Its recessive mutant allele confers a dwarf phenotype. Linkage analysis revealed that a cDNA encoding the alpha-subunit of GTP-binding protein cosegregated with d1 in 3,185 d1 segregants. Southern hybridization analysis with this cDNA as a probe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ashikari M,Wu J,Yano M,Sasaki T,Yoshimura A

更新日期：1999-08-31 00:00:00

abstract：:Mutant, but not wild-type p53 binds with high affinity to a variety of MAR-DNA elements (MARs), suggesting that MAR-binding of mutant p53 relates to the dominant-oncogenic activities proposed for mutant p53. MARs recognized by mutant p53 share AT richness and contain variations of an AATATATTT "DNA-unwinding motif," w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Will K,Warnecke G,Wiesmüller L,Deppert W

更新日期：1998-11-10 00:00:00

abstract：:The antiangiogenic agent fumagillin (Fg) and its analog TNP-470 bind to intracellular metalloprotease methionine aminopeptidase-2 (MetAP-2) and inhibit endothelial cell growth in a p53-dependent manner. To confirm the role of MetAP-2 in endothelial cell proliferation and to validate it as a physiological target for th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yeh JJ,Ju R,Brdlik CM,Zhang W,Zhang Y,Matyskiela ME,Shotwell JD,Crews CM

更新日期：2006-07-05 00:00:00

abstract：:The conformational effects of different amino acid substitutions (lysine, serine, proline, and D-valine) for glycine at position 12 in the p21 oncogene-encoded proteins have been investigated by using conformational energy calculations. The normal cellular gene codes for a glycine at position 12 in the amino acid sequ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pincus MR,Brandt-Rauf PW

更新日期：1985-06-01 00:00:00

abstract：:The study of the metabolome presents numerous challenges, first among them being the cataloging of its constituents. A step in this direction will be the development of tools to identify metabolites that share common structural features. The importance of sulfated molecules in cell-cell communication motivated us to d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mougous JD,Leavell MD,Senaratne RH,Leigh CD,Williams SJ,Riley LW,Leary JA,Bertozzi CR

更新日期：2002-12-24 00:00:00

abstract：:The insect gut microbiota plays crucial roles in modulating the interactions between the host and intestinal pathogens. Unlike viruses, bacteria, and parasites, which need to be ingested to cause disease, entomopathogenic fungi infect insects through the cuticle and proliferate in the hemolymph. However, interactions ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wei G,Lai Y,Wang G,Chen H,Li F,Wang S

更新日期：2017-06-06 00:00:00

abstract：:Two-phase random textures abound in a host of contexts, including porous and composite media, ecological structures, biological media, and astrophysical structures. Questions surrounding the spatial structure of such textures continue to pose many theoretical challenges. For example, can two-point correlation function...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jiao Y,Stillinger FH,Torquato S

更新日期：2009-10-20 00:00:00

abstract：:Seed germination is a complex trait of key ecological and agronomic significance. Few genetic factors regulating germination have been identified, and the means by which their concerted action controls this developmental process remains largely unknown. Using publicly available gene expression data from Arabidopsis th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bassel GW,Lan H,Glaab E,Gibbs DJ,Gerjets T,Krasnogor N,Bonner AJ,Holdsworth MJ,Provart NJ

更新日期：2011-06-07 00:00:00

abstract：:Focal adhesion kinase (FAK) is a nonreceptor tyrosine kinase involved in development and human disease, including cancer. It is currently thought that the four-point one, ezrin, radixin, moesin (FERM)-kinase domain linker, which contains autophosphorylation site tyrosine (Y) 397, is not required for in vivo FAK functi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heim JB,Squirewell EJ,Neu A,Zocher G,Sominidi-Damodaran S,Wyles SP,Nikolova E,Behrendt N,Saunte DM,Lock-Andersen J,Gaonkar KS,Yan H,Sarkaria JN,Krendel M,van Deursen J,Sprangers R,Stehle T,Böttcher RT,Lee JH,Ordog T

更新日期：2017-04-11 00:00:00

abstract：:The goal of this study was to characterize and classify pulmonary neuroendocrine tumors based on array comparative genomic hybridization (aCGH). Using aCGH, we performed karyotype analysis of 33 small cell lung cancer (SCLC) tumors, 13 SCLC cell lines, 19 bronchial carcinoids, and 9 gastrointestinal carcinoids. In con...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Voortman J,Lee JH,Killian JK,Suuriniemi M,Wang Y,Lucchi M,Smith WI Jr,Meltzer P,Wang Y,Giaccone G

更新日期：2010-07-20 00:00:00

abstract：:Deletion of 13 amino acids from the carboxyl terminus of the 149-amino acid staphylococcal nuclease molecule results in a denatured, partly unfolded molecule that lacks persistent secondary structure but is compact under physiological conditions. Since the modification is a carboxyl-terminal deletion, it is argued tha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Flanagan JM,Kataoka M,Shortle D,Engelman DM

更新日期：1992-01-15 00:00:00

abstract：:Inhibition of human purine nucleoside phosphorylase (PNP) stops growth of activated T-cells and the formation of 6-oxypurine bases, making it a target for leukemia, autoimmune disorders, and gout. Four generations of ribocation transition-state mimics bound to PNP are structurally characterized. Immucillin-H (K*i(1/4)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ho MC,Shi W,Rinaldo-Matthis A,Tyler PC,Evans GB,Clinch K,Almo SC,Schramm VL

更新日期：2010-03-16 00:00:00

abstract：:Toward understanding their redundancies and interactions in hemostasis and thrombosis, we examined the roles of thrombin receptors (protease-activated receptors, PARs) and the ADP receptor P2RY12 (purinergic receptor P2Y G protein-coupled 12) in human and mouse platelets ex vivo and in mouse models. Par3(-/-) and Par4...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cornelissen I,Palmer D,David T,Wilsbacher L,Concengco C,Conley P,Pandey A,Coughlin SR

更新日期：2010-10-26 00:00:00

abstract：:In a subclone derived from mouse L(tk-) cells, we found a plasmid present in a high copy number (greater than 5000 copies per cell) that was stably maintained extrachromosomally without any cytopathic effect to the host cells. This plasmid, termed L factor, has two forms: 5.3 and 5.5 kilobase pairs. DNA sequencing and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kusano T,Uehara H,Saito H,Segawa K,Oishi M

更新日期：1987-04-01 00:00:00

abstract：:Epidermal growth factor (EGF), a mitogen, also stimulates neurite extension during development, but the underlying mechanism is elusive. This study reveals a functional role for kappa opioid receptor (KOR) in EGF-stimulated neurite extension, and the underlying mechanism. EGF and activated EGF receptor (EGFR) levels a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsai NP,Tsui YC,Pintar JE,Loh HH,Wei LN

更新日期：2010-02-16 00:00:00

abstract：:Vascular flow through tissues is regulated via a number of homeostatic mechanisms. Localized control of tissue blood flow, or autoregulation, is a key factor in regulating tissue perfusion and oxygenation. We show here that the net balance between two hypoxia-inducible factor (HIF) transcription factor isoforms, HIF-1...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cowburn AS,Takeda N,Boutin AT,Kim JW,Sterling JC,Nakasaki M,Southwood M,Goldrath AW,Jamora C,Nizet V,Chilvers ER,Johnson RS

更新日期：2013-10-22 00:00:00

abstract：:Transitions in dealing with the root causes of environmental problems are advocated to achieve environmental sustainability. These transformations include (i) a demographic transition, (ii) a technology transition that includes the "green" automobile, (iii) an economic transition to one in which prices reflect full en...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Speth JG

更新日期：1992-02-01 00:00:00

abstract：:Generation of nanomechanical cantilever motion from biomolecular interactions can have wide applications, ranging from high-throughput biomolecular detection to bioactuation. Although it has been suggested that such motion is caused by changes in surface stress of a cantilever beam, the origin of the surface-stress ch...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu G,Ji H,Hansen K,Thundat T,Datar R,Cote R,Hagan MF,Chakraborty AK,Majumdar A

更新日期：2001-02-13 00:00:00

abstract：:The effect of tumor promoters on electrical coupling between human FL cells was investigated with a microelectrode technique. When a low concentration (100 ng/ml) of 12-O-tetradecanoylphorbol 13-acetate (TPA) was added to culture medium, only 6% of the cells showed electrical coupling after 5 hr, whereas in control me...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Enomoto T,Sasaki Y,Shiba Y,Kanno Y,Yamasaki H

更新日期：1981-09-01 00:00:00

abstract：:We evaluated the formation and removal of (+-)-3 alpha,4 beta-dihydroxy-1 alpha,2 alpha-epoxy-1,2,3,4- tetrahydrobenzo[c]phenanthrene (BcPHDE)-DNA adducts in two Chinese hamster ovary (CHO) cell lines. One line of repair-proficient cells (MK42) carries a stable 150-fold amplification of the dihydrofolate reductase (DH...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carothers AM,Zhen W,Mucha J,Zhang YJ,Santella RM,Grunberger D,Bohr VA

更新日期：1992-12-15 00:00:00

abstract：:Global loss of DNA methylation has been known for decades as an epigenomic aberration associated with carcinogenesis and cancer progression. Loss of DNA methylation affects predominantly repetitive elements, which encompass >50% of the CpG dinucleotides present in the human genome. Because of the lack of an effective ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xie H,Wang M,Bonaldo Mde F,Rajaram V,Stellpflug W,Smith C,Arndt K,Goldman S,Tomita T,Soares MB

更新日期：2010-04-13 00:00:00

abstract：:Intergroup conflict persists when and because individuals make costly contributions to their group's fighting capacity, but how groups organize contributions into effective collective action remains poorly understood. Here we distinguish between contributions aimed at subordinating out-groups (out-group aggression) fr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： De Dreu CK,Gross J,Méder Z,Giffin M,Prochazkova E,Krikeb J,Columbus S

更新日期：2016-09-20 00:00:00

abstract：:Programmed death-1 (PD-1) regulates T cell exhaustion during chronic infections. Blocking the PD-1:PD-ligand (PD-L) pathway reinvigorates exhausted CD8 T cells. Exactly how blocking PD-1:PD-L interactions improves T cell immunity, however, remains unclear. PD-1:PD-L blockade could reprogram all exhausted T cells to be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Blackburn SD,Shin H,Freeman GJ,Wherry EJ

更新日期：2008-09-30 00:00:00