Abstract:
:The cellular response to ionizing radiation (IR) includes induction of the c-jun and EGR1 early response genes. The present work has examined potential cytoplasmic signaling cascades that transduce IR-induced signals to the nucleus. The results demonstrate activation of the 40S ribosomal protein S6 kinase, pp90rsk, in human U-937 myeloid leukemia cells. Partial purification of pp90rsk by affinity chromatography demonstrated an increase in S6 peptide phosphorylation when comparing irradiated with control cells. IR-induced activation of pp90rsk was further confirmed in immune-complex kinase assays. In contrast to these findings, there was no detectable induction of pp70S6K. Previous work has demonstrated that mitogen-activated protein kinase activates pp90rsk. The present results further show that IR treatment is associated with induction of mitogen-activated protein kinase activity and that this event is temporally related to activation of pp90rsk and early response gene expression. These findings suggest that activation of the mitogen-activated protein kinase/pp90rsk cascade is involved in the response of cells to IR exposure.
journal_name
Proc Natl Acad Sci U S Aauthors
Kharbanda S,Saleem A,Shafman T,Emoto Y,Weichselbaum R,Kufe Ddoi
10.1073/pnas.91.12.5416subject
Has Abstractpub_date
1994-06-07 00:00:00pages
5416-20issue
12eissn
0027-8424issn
1091-6490journal_volume
91pub_type
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2017-02-14 00:00:00