Origin of nanomechanical cantilever motion generated from biomolecular interactions.

abstract：:We have studied the expression of simian virus 40 (SV40) specific tumor antigen (T-antigen) and viral RNA in SV40-transformed mouse 3T3 cells that are temperature-sensitive for the expression of the transformed phenotype (ts SV3T3). Although transformed by wild-type SV40, ts SV3T3 cells at 32 degrees behave like stand...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Basilico C,Zouzias D

更新日期：1976-06-01 00:00:00

abstract：:Tight junctions (TJs) play a key role in mediating paracellular ion reabsorption in the kidney. The paracellular pathway in the collecting duct of the kidney is a predominant route for transepithelial chloride reabsorption that determines the extracellular NaCl content and the blood pressure. However, the molecular me...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hou J,Renigunta A,Yang J,Waldegger S

更新日期：2010-10-19 00:00:00

abstract：:The motor protein cytoplasmic dynein is responsible for most of the minus-end-directed microtubule traffic within cells. Dynein contains four evolutionarily conserved AAA (ATPase associated with various cellular activities) domains that are thought to bind nucleotide; the role of nucleotide binding and hydrolysis in e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reck-Peterson SL,Vale RD

更新日期：2004-02-10 00:00:00

abstract：:In this paper we solve several boundary value problems for the Helmholtz equation on polygonal domains. We observe that when the problems are formulated as the boundary integral equations of potential theory, the solutions are representable by series of appropriately chosen Bessel functions. In addition to being analy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Serkh K,Rokhlin V

更新日期：2016-08-16 00:00:00

abstract：:The neu gene is distantly related to the erbB gene and encodes a cell surface protein that appears to function as a growth factor receptor. To study the mechanisms that caused the conversion of the normal neu gene to an oncogenic allele, we have isolated molecular clones of the neu oncogene as well as a clone of the c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hung MC,Schechter AL,Chevray PY,Stern DF,Weinberg RA

更新日期：1986-01-01 00:00:00

abstract：:Neuronal signal integration as well as synaptic transmission and plasticity highly depend on the morphology of dendrites and their spines. Nogo-A is a membrane protein enriched in the adult central nervous system (CNS) myelin, where it restricts the capacity of axons to grow and regenerate after injury. Nogo-A is also...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Petrinovic MM,Hourez R,Aloy EM,Dewarrat G,Gall D,Weinmann O,Gaudias J,Bachmann LC,Schiffmann SN,Vogt KE,Schwab ME

更新日期：2013-01-15 00:00:00

abstract：:Brief learning experience (classical conditioning) induces frequency-specific receptive-field (RF) plasticity in the auditory cortex, characterized as increased response to the frequency of the conditioned stimulus and decreased responses to most other frequencies, including the pretraining best frequency. This experi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weinberger NM,Javid R,Lepan B

更新日期：1993-03-15 00:00:00

abstract：:Yeast RNA polymerase II initiation factor e was purified to homogeneity and identified by biochemical criteria as the counterpart of human transcription factor IIB. Factor e was essential for initiation of transcription from yeast and mammalian promoters in a reconstituted yeast transcription system. Activity resided ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tschochner H,Sayre MH,Flanagan PM,Feaver WJ,Kornberg RD

更新日期：1992-12-01 00:00:00

abstract：:The structurally diverse peroxisome proliferators ciprofibrate, clofibrate, and bis(2-ethylhexyl) phthalate [(EtHx)2 greater than Pht] increase the activities of hepatic catalase and peroxisomal fatty acid beta-oxidation enzymes in conjunction with profound proliferation of peroxisomes in hepatocytes. In order to deli...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reddy JK,Goel SK,Nemali MR,Carrino JJ,Laffler TG,Reddy MK,Sperbeck SJ,Osumi T,Hashimoto T,Lalwani ND

更新日期：1986-03-01 00:00:00

abstract：:A neuroethological approach to human and nonhuman primate behavior and cognition predicts biological specializations for social life. Evidence reviewed here indicates that ancestral mechanisms are often duplicated, repurposed, and differentially regulated to support social behavior. Focusing on recent research from no...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang SW,Brent LJ,Adams GK,Klein JT,Pearson JM,Watson KK,Platt ML

更新日期：2013-06-18 00:00:00

abstract：:Nine subjects were studied by high-speed magnetic resonance imaging while performing language-based tasks. Subjects were asked either to repeat or to generate verbs associated with nouns read by an experimenter while magnetic resonance images were obtained of the left inferior frontal lobe. The echo-planar imaging seq...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McCarthy G,Blamire AM,Rothman DL,Gruetter R,Shulman RG

更新日期：1993-06-01 00:00:00

abstract：:This paper reports a general in situ method to grow a polymer conjugate solely from the C terminus of a recombinant protein. GFP was fused at its C terminus with an intein; cleavage of the intein provided a unique thioester moiety at the C terminus of GFP that was used to install an atom transfer radical polymerizatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao W,Liu W,Christensen T,Zalutsky MR,Chilkoti A

更新日期：2010-09-21 00:00:00

abstract：:A major goal for developmental biologists is to define the behaviors and molecular contents of differentiating cells. We have devised a strategy for isolating cells from diverse embryonic regions and stages in the zebrafish, using computer-guided laser photoconversion of injected Kaede protein and flow cytometry. This...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brown JL,Snir M,Noushmehr H,Kirby M,Hong SK,Elkahloun AG,Feldman B

更新日期：2008-08-26 00:00:00

abstract：:Phagocytes are a critical component of the innate immune response in humans and eliminate invading microorganisms through a process known as phagocytosis. Two distinct receptor-linked phagocytic pathways, one with Ab receptors (FcRs; FcR, Fc receptor) and the other complement receptors (CRs), mediate binding and inges...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kobayashi SD,Voyich JM,Buhl CL,Stahl RM,DeLeo FR

更新日期：2002-05-14 00:00:00

abstract：:Previous studies have demonstrated that mutation in the forkhead domain of the forkhead box P2 (FOXP2) protein (R553H) causes speech-language disorders. To further analyze FOXP2 function in speech learning, we generated a knockin (KI) mouse for Foxp2 (R552H) [Foxp2 (R552H)-KI], corresponding to the human FOXP2 (R553H)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fujita E,Tanabe Y,Shiota A,Ueda M,Suwa K,Momoi MY,Momoi T

更新日期：2008-02-26 00:00:00

abstract：:Reversible ligands were attached covalently to membrane-bound acetylcholine receptor from Torpedo marmorata by a method which is generally applicable and does not require the synthesis of specially designed molecules. UV irradiation of the receptor in the presence of [3H]trimethisoquin, [3H]phencyclidine, or [3H]perhy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oswald R,Changeux JP

更新日期：1981-06-01 00:00:00

abstract：:Monoclonal antibodies penetrate bulky tumors poorly after intravenous administration, in part because of specific binding to the target antigen. Experiments presented here demonstrate an analogous phenomenon in micrometastases; poor antibody penetration, attributable to a "binding-site barrier" phenomenon, can be seen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Saga T,Neumann RD,Heya T,Sato J,Kinuya S,Le N,Paik CH,Weinstein JN

更新日期：1995-09-12 00:00:00

abstract：:Rats were raised after weaning on a vitamin D-deficient diet which used whole wheat and casein as the major protein source. For at least the first year of life, plasma calcium concentrations of these rats were the same as those of vitamin D-replete rats, and the rate of growth was normal for at least 6 months. The fol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lester GE,VanderWiel CJ,Gray TK,Talmage RV

更新日期：1982-08-01 00:00:00

abstract：:In most genome-wide linkage studies, implication of a causative disease gene often requires years of expanding the study to more families and finer mapping of the initially described region. Even after such efforts, unobtainable sample sizes can be required to make statistically meaningful conclusions about a single g...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Antinozzi PA,Garcia-Diaz A,Hu C,Rothman JE

更新日期：2006-03-07 00:00:00

abstract：:The development of the luminescence system in Beneckea harveyi is controlled by cyclic nucleotides at the level of transcription. In the wild type, it is repressed by exogenously added guanosine 3':5'-cyclic monophosphate and this repression is overcome by the addition of adenosine 3':5'-cyclic monophosphate. These ob...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ulitzur S,Yashphe J,Hastings JW

更新日期：1976-12-01 00:00:00

abstract：:Multiple drug resistance of tumor cells is a common problem in cancer therapy. We have demonstrated that membrane vesicles from highly multidrug-resistant human KB carcinoma cell lines exhibit increased specific and saturable binding of vinblastine. To identify the molecules that bind vinblastine, membrane vesicles fr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cornwell MM,Safa AR,Felsted RL,Gottesman MM,Pastan I

更新日期：1986-06-01 00:00:00

abstract：:Characteristic classes for the index of the Dirac family [unk](A) are computed in terms of differential forms on the orbit space of vector potentials under gauge transformations. They represent obstructions to the existence of a covariant Dirac propagator. The first obstruction is related to a chiral anomaly. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Atiyah MF,Singer IM

更新日期：1984-04-01 00:00:00

abstract：:The role of the mouse tumor necrosis factor (TNF) promoter, 5' untranslated region (UTR), and 3' UTR in TNF gene expression has been examined in three nonmacrophage cell lines (HeLa, NIH 3T3, and L-929). The TNF promoter is not macrophage-specific. On the contrary, it constitutively drives reporter gene expression in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kruys V,Kemmer K,Shakhov A,Jongeneel V,Beutler B

更新日期：1992-01-15 00:00:00

abstract：:Finding regions of the genome that are significantly recurrent in noisy data are a common but difficult problem in present day computational biology. Cores of recurrent events (CORE) is a computational approach to solving this problem that is based on a formalized notion by which "core" intervals explain the observed ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krasnitz A,Sun G,Andrews P,Wigler M

更新日期：2013-06-18 00:00:00

abstract：:The major histocompatibility complex-encoded class I molecule, a noncovalent dimer of a polymorphic 45-kDa heavy chain and a nonpolymorphic 12-kDa beta 2-microglobulin (beta 2m) light chain, binds peptide antigen prior to its interaction with T-cell antigen receptors. We report here that the binding in aqueous solutio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boyd LF,Kozlowski S,Margulies DH

更新日期：1992-03-15 00:00:00

abstract：:To facilitate analysis of plant cell wall polysaccharide structure and composition, we cloned 74 genes encoding polysaccharide-degrading enzymes from Aspergillus nidulans, Aspergillus fumigatus, and Neurospora crassa and expressed the genes as secreted proteins with C-terminal Myc and 6x His tags. Most of the recombin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bauer S,Vasu P,Persson S,Mort AJ,Somerville CR

更新日期：2006-07-25 00:00:00

abstract：:Ca(2+) is known to have important roles in sperm chemotaxis, although the relationship between intracellular Ca(2+) concentration ([Ca(2+)](i)) and modulation of the swimming and chemotactic behavior of spermatozoa has not been elucidated. Using a high-speed Ca(2+) imaging system, we examined the chemotactic behavior ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shiba K,Baba SA,Inoue T,Yoshida M

更新日期：2008-12-09 00:00:00

abstract：:For species to stay temporally tuned to their environment, they use cues such as the accumulation of degree-days. The relationships between the timing of a phenological event in a population and its environmental cue can be described by a population-level reaction norm. Variation in reaction norms along environmental ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Delgado MDM,Roslin T,Tikhonov G,Meyke E,Lo C,Gurarie E,Abadonova M,Abduraimov O,Adrianova O,Akimova T,Akkiev M,Ananin A,Andreeva E,Andriychuk N,Antipin M,Arzamascev K,Babina S,Babushkin M,Bakin O,Barabancova A,Bas

更新日期：2020-12-08 00:00:00

abstract：:Muscarinic acetylcholine receptors (mAChR) in the embryonic chicken heart undergo agonist-induced internalization and a subsequent decrease in receptor number (downregulation). Cloning studies have identified two subtypes of mAChR expressed in the embryonic chicken heart, the cm2 and cm4 receptors. We report here that...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Habecker BA,Nathanson NM

更新日期：1992-06-01 00:00:00

abstract：:Cell-free DNA shed by cancer cells has been shown to be a rich source of putative tumor-specific biomarkers. Because cell-free DNA from brain and spinal cord tumors cannot usually be detected in the blood, we studied whether the cerebrospinal fluid (CSF) that bathes the CNS is enriched for tumor DNA, here termed CSF-t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang Y,Springer S,Zhang M,McMahon KW,Kinde I,Dobbyn L,Ptak J,Brem H,Chaichana K,Gallia GL,Gokaslan ZL,Groves ML,Jallo GI,Lim M,Olivi A,Quinones-Hinojosa A,Rigamonti D,Riggins GJ,Sciubba DM,Weingart JD,Wolinsky JP

更新日期：2015-08-04 00:00:00