Abstract:
:To facilitate analysis of plant cell wall polysaccharide structure and composition, we cloned 74 genes encoding polysaccharide-degrading enzymes from Aspergillus nidulans, Aspergillus fumigatus, and Neurospora crassa and expressed the genes as secreted proteins with C-terminal Myc and 6x His tags. Most of the recombinant enzymes were active in enzyme assays, and optima for pH and temperature were established. A subset of the enzymes was used to fragment polysaccharides from the irregular xylem 9 (irx9) mutant of Arabidopsis. The analysis revealed a decrease in the abundance of xylan in the mutant, indicating that the IRX9 gene, which encodes a putative family 43 glycosyltransferase, is required for xylan synthesis.
journal_name
Proc Natl Acad Sci U S Aauthors
Bauer S,Vasu P,Persson S,Mort AJ,Somerville CRdoi
10.1073/pnas.0604632103subject
Has Abstractpub_date
2006-07-25 00:00:00pages
11417-22issue
30eissn
0027-8424issn
1091-6490pii
0604632103journal_volume
103pub_type
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