Abstract:
:Yeast RNA polymerase II initiation factor e was purified to homogeneity and identified by biochemical criteria as the counterpart of human transcription factor IIB. Factor e was essential for initiation of transcription from yeast and mammalian promoters in a reconstituted yeast transcription system. Activity resided in a single polypeptide of approximately 41 kDa, identified by peptide sequence analysis as the product of the SUA7 gene. Factor e interacted specifically with RNA polymerase II, consistent with a proposed role in determining the start site of transcription.
journal_name
Proc Natl Acad Sci U S Aauthors
Tschochner H,Sayre MH,Flanagan PM,Feaver WJ,Kornberg RDdoi
10.1073/pnas.89.23.11292keywords:
subject
Has Abstractpub_date
1992-12-01 00:00:00pages
11292-6issue
23eissn
0027-8424issn
1091-6490journal_volume
89pub_type
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