Effects of an HIV-1 maturation inhibitor on the structure and dynamics of CA-SP1 junction helices in virus-like particles.

abstract：:We have isolated and characterized the complete human villin gene. The villin gene is located on chromosome 2q35-36 in humans and on chromosome 1 in mice. Villin belongs to a family of calcium-regulated actin-binding proteins that share structural and functional homologies. The villin gene is expressed mainly in cells...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pringault E,Robine S,Louvard D

更新日期：1991-12-01 00:00:00

abstract：:TCL1 and TCL1b genes on human chromosome 14q23.1 are activated in T cell leukemias by translocations and inversions at 14q32.1, juxtaposing them to regulatory elements of T cell receptor genes. In this report we present the cloning, mapping, and expression analysis of the human and murine TCL1/Tcl1 locus. In addition ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hallas C,Pekarsky Y,Itoyama T,Varnum J,Bichi R,Rothstein JL,Croce CM

更新日期：1999-12-07 00:00:00

abstract：:Controversy remains over whether the cancer stem cell (CSC) theory applies to all tumors. To determine whether cells within a highly aggressive solid tumor are stochastically or hierarchically organized, we combined a reporter system where the nucleostemin (NS) promoter drives GFP expression (termed NS-GFP) with a mou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tamase A,Muraguchi T,Naka K,Tanaka S,Kinoshita M,Hoshii T,Ohmura M,Shugo H,Ooshio T,Nakada M,Sawamoto K,Onodera M,Matsumoto K,Oshima M,Asano M,Saya H,Okano H,Suda T,Hamada J,Hirao A

更新日期：2009-10-06 00:00:00

abstract：:The development of a spider silk-manufacturing process is of great interest. However, there are serious problems with natural manufacturing through spider farming, and standard recombinant protein production platforms have provided limited progress due to their inability to assemble spider silk proteins into fibers. T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Teulé F,Miao YG,Sohn BH,Kim YS,Hull JJ,Fraser MJ Jr,Lewis RV,Jarvis DL

更新日期：2012-01-17 00:00:00

abstract：:In signal-recognition particle (SRP)-dependent protein targeting to the bacterial plasma membrane, two GTPases, Ffh (a subunit of the bacterial SRP) and FtsY (the bacterial SRP receptor), act as GTPase activating proteins for one another. The molecular mechanism of this reciprocal GTPase activation is poorly understoo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shan SO,Walter P

更新日期：2003-04-15 00:00:00

abstract：:Tail-anchored (TA) membrane proteins are involved in a variety of important cellular functions, including membrane fusion, protein translocation, and apoptosis. The ATPase Get3 (Asna1, TRC40) was identified recently as the endoplasmic reticulum targeting factor of TA proteins. Get3 consists of an ATPase and alpha-heli...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bozkurt G,Stjepanovic G,Vilardi F,Amlacher S,Wild K,Bange G,Favaloro V,Rippe K,Hurt E,Dobberstein B,Sinning I

更新日期：2009-12-15 00:00:00

abstract：:Phytocannabinoids, such as the principal bioactive component of marijuana, delta9-tetrahydrocannabinol, have been used for thousands of years for medical and recreational purposes. delta9-Tetrahydrocannabinol and endogenous cannabinoids (e.g., anandamide) initiate their agonist properties by stimulating the cannabinoi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moore SA,Nomikos GG,Dickason-Chesterfield AK,Schober DA,Schaus JM,Ying BP,Xu YC,Phebus L,Simmons RM,Li D,Iyengar S,Felder CC

更新日期：2005-12-06 00:00:00

abstract：:The exopolysaccharide alginate is a major virulence factor in the pathogenicity of Pseudomonas aeruginosa infecting the lungs of cystic fibrosis (CF) patients. Alginate synthesis by P. aeruginosa is believed to occur in response to environmental signals present in the CF lung. Transcription of a critical alginate bios...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roychoudhury S,Sakai K,Chakrabarty AM

更新日期：1992-04-01 00:00:00

abstract：:Electron transfer dissociation (ETD) is a recently introduced mass spectrometric technique that provides a more comprehensive coverage of peptide sequences and posttranslational modifications. Here, we evaluated the use of ETD for a global phosphoproteome analysis. In all, we identified a total of 1,435 phosphorylatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Molina H,Horn DM,Tang N,Mathivanan S,Pandey A

更新日期：2007-02-13 00:00:00

abstract：:I demonstrate the existence in protein domain sequences of sets of statistically significant periodic signals, characteristic of the architectures of those domains. It is shown that, although the frequencies of the signals characteristic of a particular architecture have definite wave numbers, they can occur in any ph...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rackovsky S

更新日期：1998-07-21 00:00:00

abstract：:DPB11, a gene that suppresses mutations in two essential subunits of Saccharomyces cerevisiae DNA polymerase II(epsilon) encoded by POL2 and DPB2, was isolated on a multicopy plasmid. The nucleotide sequence of the DPB11 gene revealed an open reading frame predicting an 87-kDa protein. This protein is homologous to th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Araki H,Leem SH,Phongdara A,Sugino A

更新日期：1995-12-05 00:00:00

abstract：:Regulation of gene expression through translational control is a fundamental mechanism implicated in many biological processes ranging from memory formation to innate immunity and whose dysregulation contributes to human diseases. Genome wide analyses of translational control strive to identify differential translatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Larsson O,Sonenberg N,Nadon R

更新日期：2010-12-14 00:00:00

abstract：:The plant hormones abscisic acid (ABA) and gibberellic acid (GA) are important regulators of the dormancy and germination of seeds. In cereals, GA enhances the synthesis and secretion of enzymes (principally alpha-amylases) in the aleurone cells of the endosperm, which then mobilize the storage reserves that fuel germ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ritchie S,Gilroy S

更新日期：1998-03-03 00:00:00

abstract：:We have constructed three different fusion genes containing the herpes simplex virus thymidine kinase (HSV tk) and the bacterial neomycin phosphotransferase (neo) genes. All three fusion genes utilize the HSV tk promoter but differ at the junction of their components. We have determined if the fusion genes are bifunct...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schwartz F,Maeda N,Smithies O,Hickey R,Edelmann W,Skoultchi A,Kucherlapati R

更新日期：1991-12-01 00:00:00

abstract：:The generalized seizures of status epilepticus (SE) trigger a series of molecular and cellular events that produce cognitive deficits and can culminate in the development of epilepsy. Known early events include opening of the blood-brain barrier (BBB) and astrocytosis accompanied by activation of brain microglia. Wher...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Varvel NH,Neher JJ,Bosch A,Wang W,Ransohoff RM,Miller RJ,Dingledine R

更新日期：2016-09-20 00:00:00

abstract：:The ability of antibodies to extravasate out of blood vessels is critical for therapeutic activity, because molecular targets for most diseases are located outside of the endothelial lining. By performing detailed biodistribution studies with a novel IL9-armed cancer-specific antibody, we identified a clear correlatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Venetz D,Hess C,Lin CW,Aebi M,Neri D

更新日期：2015-02-17 00:00:00

abstract：:Epitopes for 22 alloantibodies that inhibit factor VIII procoagulant protein (FVIII) from multitransfused individuals with severe hemophilia A and three autoantibodies from nonhemophilic individuals appeared to be restricted to two specific regions of the FVIII molecule. Immunoblotting of purified FVIII and purified t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fulcher CA,de Graaf Mahoney S,Roberts JR,Kasper CK,Zimmerman TS

更新日期：1985-11-01 00:00:00

abstract：:Complementary DNA (cDNA) clones encoding the adult human liver flavin-containing monooxygenase (FMO; dimethylaniline N-oxidase, EC 1.14.13.8) were isolated from lambda gt10 and lambda gt11 libraries. The cDNA libraries were screened with three synthetic 36-mer oligonucleotide probes derived from the nucleic acid seque...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lomri N,Gu Q,Cashman JR

更新日期：1992-03-01 00:00:00

abstract：:The boreal forests, identified as a critical "tipping element" of the Earth's climate system, play a critical role in the global carbon budget. Recent findings have suggested that terrestrial carbon sinks in northern high-latitude regions are weakening, but there has been little observational evidence to support the i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ma Z,Peng C,Zhu Q,Chen H,Yu G,Li W,Zhou X,Wang W,Zhang W

更新日期：2012-02-14 00:00:00

abstract：:DNA repair alkyltransferases protect organisms against the cytotoxic, mutagenic, and carcinogenic effects of alkylating agents by transferring alkyl adducts from DNA to an active cysteine on the protein, thereby restoring the native DNA structure. We used random sequence substitutions to gain structure-function inform...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Christians FC,Loeb LA

更新日期：1996-06-11 00:00:00

abstract：:Glu-126 (helix IV) and Arg-144 (helix V) are charge paired and play a critical role in substrate binding in the lactose permease of Escherichia coli. When Glu-126 is replaced with Asp, the permease has relatively high activity, implying that helix V has sufficient flexibility to allow Arg-144 to accommodate the decrea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weinglass AB,Kaback HR

更新日期：1999-09-28 00:00:00

abstract：:Monotremes have left a poor fossil record, and paleontology has been virtually mute during two decades of discussion about molecular clock estimates of the timing of divergence between the platypus and echidna clades. We describe evidence from high-resolution x-ray computed tomography indicating that Teinolophos, an E...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rowe T,Rich TH,Vickers-Rich P,Springer M,Woodburne MO

更新日期：2008-01-29 00:00:00

abstract：:Ferredoxin:NADPH oxidoreductase (FNR) is a key enzyme of photosynthetic electron transport required for generation of reduction equivalents. Recently, two proteins were found to be involved in membrane-anchoring of FNR by specific interaction via a conserved Ser/Pro-rich motif: Tic62 and Trol. Our crystallographic stu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alte F,Stengel A,Benz JP,Petersen E,Soll J,Groll M,Bölter B

更新日期：2010-11-09 00:00:00

abstract：:Astronomers and physicists noticed centuries ago that visual spatial resolution is higher for dark than light stimuli, but the neuronal mechanisms for this perceptual asymmetry remain unknown. Here we demonstrate that the asymmetry is caused by a neuronal nonlinearity in the early visual pathway. We show that neurons ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kremkow J,Jin J,Komban SJ,Wang Y,Lashgari R,Li X,Jansen M,Zaidi Q,Alonso JM

更新日期：2014-02-25 00:00:00

abstract：:Previously we uncovered a critical role for norepinephrine and beta(1)-adrenergic signaling in hippocampus-dependent memory retrieval. Because the beta(1) receptor couples to G(s), we examine here whether cAMP is also required for contextual memory retrieval. Using pharmacologic and genetic approaches to manipulate cA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ouyang M,Zhang L,Zhu JJ,Schwede F,Thomas SA

更新日期：2008-08-19 00:00:00

abstract：:The first step in the replication of the adenovirus genome is the covalent attachment of the 5'-terminal nucleotide, dCMP, to the virus-encoded terminal protein precursor (pTP). This reaction can be observed in vitro and has been previously shown to be dependent upon either viral DNA or linearized plasmid DNA containi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Challberg MD,Rawlins DR

更新日期：1984-01-01 00:00:00

abstract：:Systemic administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) causes parkinsonism in humans and subhuman primates, but not in rats and many other laboratory animals; mice are intermediate in their susceptibility. Since MPTP causes selective dopaminergic neurotoxicity when infused directly into rat sub...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kalaria RN,Mitchell MJ,Harik SI

更新日期：1987-05-01 00:00:00

abstract：:The molecular mechanisms by which transcription is selectively activated and precisely controlled by signal transducer and activator of transcription (Stat) factors represent a central issue in cytokine-mediated cellular responses. Stat6 mediates responses to IL-4 and antagonizes Stat1 activated by IFN-gamma. We have ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goenka S,Boothby M

更新日期：2006-03-14 00:00:00

abstract：:Here, using further optimized 3D culture that allows highly selective induction and long-term growth of human ES cell (hESC)-derived cortical neuroepithelium, we demonstrate unique aspects of self-organization in human neocorticogenesis. Self-organized cortical tissue spontaneously forms a polarity along the dorsocaud...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kadoshima T,Sakaguchi H,Nakano T,Soen M,Ando S,Eiraku M,Sasai Y

更新日期：2013-12-10 00:00:00

abstract：:Therapy-related acute myeloid leukemias (t-AML) with translocations of the MLL gene are associated with the use of topoisomerase II inhibitors. We established the emergence of the malignant clone in a child who developed t-AML with a t(11;19) (q23;p13.3) during treatment for acute lymphoblastic leukemia (ALL). The MLL...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Blanco JG,Dervieux T,Edick MJ,Mehta PK,Rubnitz JE,Shurtleff S,Raimondi SC,Behm FG,Pui CH,Relling MV

更新日期：2001-08-28 00:00:00