Exploring the aggregation free energy landscape of the amyloid-β protein (1-40).

abstract：:By affinity chromatography on heparin-Sepharose, two classes of lipoproteins were separated from high density lipoproteins (HDL) isolated from patients with primary or secondary lecithin:cholesterol acyltransferase (LCATase; EC 2.3.1.43) deficiency and from normal subjects. The unretained fraction, HDL(A), was charact...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marcel YL,Vezina C,Emond D,Suzue G

更新日期：1980-05-01 00:00:00

abstract：:Bones from a stratified sedimentary deposit in the Puu Naio Cave site on Maui, Hawaiian Islands, reveal the late Holocene extinction of 19 species of birds. The age of the sediment and associated fauna was determined by direct radiocarbon dating (tandem particle accelerator-mass spectrometer; TAMS) of amino acids extr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： James HF,Stafford TW Jr,Steadman DW,Olson SL,Martin PS,Jull AJ,McCoy PC

更新日期：1987-04-01 00:00:00

abstract：:Homeodomain-interacting protein kinase-2 (HIPK2) is a serine/threonine kinase involved in transcriptional regulation and apoptosis. The transcriptional corepressor CtBP (carboxyl-terminal binding protein) also plays a fundamental role in these processes. Our previous studies indicate that HIPK2 participates in a pathw...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang Q,Nottke A,Goodman RH

更新日期：2005-02-22 00:00:00

abstract：:Rapid electrical conduction in the His-Purkinje system tightly controls spatiotemporal activation of the ventricles. Although recent work has shed much light on the regulation of early specification and morphogenesis of the His-Purkinje system, less is known about how transcriptional regulation establishes impulse con...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang SS,Kim KH,Rosen A,Smyth JW,Sakuma R,Delgado-Olguín P,Davis M,Chi NC,Puviindran V,Gaborit N,Sukonnik T,Wylie JN,Brand-Arzamendi K,Farman GP,Kim J,Rose RA,Marsden PA,Zhu Y,Zhou YQ,Miquerol L,Henkelman RM,Sta

更新日期：2011-08-16 00:00:00

abstract：:Establishing quantitative relationships between molecular structure and broad biological effects has been a longstanding challenge in science. Currently, no method exists for forecasting broad biological activity profiles of medicinal agents even within narrow boundaries of structurally similar molecules. Starting fro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fliri AF,Loging WT,Thadeio PF,Volkmann RA

更新日期：2005-01-11 00:00:00

abstract：:Chromopeptides were prepared by proteolytic digestion of phytochrome (far-red absorbing form, P(fr)) and of phycocyanin. The phycocyanobilin peptide, the chromophore of which is Z,Z,Z-configurated, was modified to the Z,Z,E isomeric chromophore. It has been demonstrated earlier that the P(fr) chromopeptide and the Z,Z...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rüdiger W,Thümmler F,Cmiel E,Schneider S

更新日期：1983-10-01 00:00:00

abstract：:Reactive oxygen species (ROS) are known to be important signal molecules that are involved in biotic and abiotic stress responses as well as in growth regulation. However, the molecular mechanisms by which ROS act as a growth regulator, as well as how ROS-dependent growth regulation relates to its roles in stress resp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mabuchi K,Maki H,Itaya T,Suzuki T,Nomoto M,Sakaoka S,Morikami A,Higashiyama T,Tada Y,Busch W,Tsukagoshi H

更新日期：2018-05-15 00:00:00

abstract：:We describe the effect of a 200-microns wavelength free electron laser beam on the ability of asynchronized and synchronized mammalian tissue culture cells to incorporate tritiated thymidine. Compared to controls (unexposed cells), a significant proportion of exposed cells exhibited a reduction in isotope incorporatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berns MW,Bewley W,Sun CH,Templin P

更新日期：1990-04-01 00:00:00

abstract：:Cholera vibrios produce a single polymeric protein that (i) causes hemagglutination; (ii) appears to participate in their attachment to gut epithelium; (iii) may mediate their detachment from gut epithelium; and (iv) is a protease that hydrolyzes fibronectin and mucin, cleaves lactoferrin, and nicks the A subunit of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Finkelstein RA,Boesman-Finkelstein M,Holt P

更新日期：1983-02-01 00:00:00

abstract：:The receptor for acetylcholine was partially purified by affinity chromatography of an extract in Triton X-100 of membrane fragments from electric tissue. The receptor was assayed, after its reduction with dithiothreitol, by reaction with the affinity-alkylating agent, [methyl-(3)H]4-(N-maleimido)-benzyltrimethylammon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karlin A,Cowburn D

更新日期：1973-12-01 00:00:00

abstract：:In most human follicular B cell lymphomas the bcl-2 gene is up-regulated as a result of the t(14;18) chromosomal translocation generating a hybrid bcl-2-IgH mRNA. Recently, we have identified in t(14;18)-positive cells a bcl-2-IgH mRNA in the antisense orientation, putatively responsible for the overexpression of bcl-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morelli S,Delia D,Capaccioli S,Quattrone A,Schiavone N,Bevilacqua A,Tomasini S,Nicolin A

更新日期：1997-07-22 00:00:00

abstract：:We report an approach for studying protein-induced DNA bends in solution that is based on measuring the sizes of circular DNA molecules by using two-dimensional gel electrophoresis. These circular fragments are obtained by ligating short synthetic oligonucleotides containing a protein-recognition region in the presenc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lyubchenko Y,Shlyakhtenko L,Chernov B,Harrington RE

更新日期：1991-06-15 00:00:00

abstract：:A general procedure is described for the production of human monoclonal antibodies from peripheral blood lymphocytes immunized in vitro against T-cell-dependent antigens. These lymphocytes immunized in culture were used to produce human-human or human-mouse hybridomas secreting monoclonal antibodies specific for digox...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Borrebaeck CA,Danielsson L,Möller SA

更新日期：1988-06-01 00:00:00

abstract：:Serotonin (5-HT) plays a key role in early brain development, and manipulation of 5-HT levels during this period can have lasting neurobiological and behavioral consequences. It is unclear how perinatal exposure to drugs, such as selective serotonin reuptake inhibitors (SSRIs), impacts cortical neural network function...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Simpson KL,Weaver KJ,de Villers-Sidani E,Lu JY,Cai Z,Pang Y,Rodriguez-Porcel F,Paul IA,Merzenich M,Lin RC

更新日期：2011-11-08 00:00:00

abstract：:Multiple biological processes involve the stretching of nucleic acids (NAs). Stretching forces induce local changes in the molecule structure, inhibiting or promoting the binding of proteins, which ultimately affects their functionality. Understanding how a force induces changes in the structure of NAs at the atomic l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marin-Gonzalez A,Vilhena JG,Perez R,Moreno-Herrero F

更新日期：2017-07-03 00:00:00

abstract：:Parathyroid hormone (PTH) is central to calcium homeostasis and bone maintenance in vertebrates, and as such it has been used for treating osteoporosis. It acts primarily by binding to its receptor, PTH1R, a member of the class B G protein-coupled receptor (GPCR) family that also includes receptors for glucagon, calci...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pioszak AA,Xu HE

更新日期：2008-04-01 00:00:00

abstract：:Gentamicin C complex from Micromonospora echinospora remains a globally important antibiotic, and there is revived interest in the semisynthesis of analogs that might show improved therapeutic properties. The complex consists of five components differing in their methylation pattern at one or more sites in the molecul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li S,Guo J,Reva A,Huang F,Xiong B,Liu Y,Deng Z,Leadlay PF,Sun Y

更新日期：2018-02-06 00:00:00

abstract：:Loss of ATP accompanying accumulation of dATP has recently been reported to occur in the erythrocytes and lymphoblasts of patients with T lymphocytic leukemia during treatment with deoxycoformycin, an inhibitor of adenosine deaminase (adenosine aminohydrolase, EC 3.5.4.4) that causes the accumulation of deoxyadenosine...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bagnara AS,Hershfield MS

更新日期：1982-04-01 00:00:00

abstract：:The 158,000 Mr protein, previously designated C5c, present in fully assembled complement (C) membrane attack complexes (MC5b-9) has been identified as a disulfide-bonded dimer of C9. This conclusion was based on the observations that: (i) a portion of the 125I-radiolabeled precursor C9 incorporated into MC5b-9 complex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ware CF,Kolb WP

更新日期：1981-10-01 00:00:00

abstract：:A 16-amino acid residue peptide derived from a consensus motif of natural ferredoxins incorporates a tetranuclear iron sulfur cluster under physiological conditions. Successful assembly of the [4Fe-4S]2+/1+ cluster within a monomeric peptide was demonstrated using size exclusion chromatography, UV-visible, visible CD,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gibney BR,Mulholland SE,Rabanal F,Dutton PL

更新日期：1996-12-24 00:00:00

abstract：:Treatment of myoblast cell lines with 3-deazaadenosine stimulates differentiation into myofibers. Myoblast clone L5/ 3B5 , which does not form myofibers after 6 days in fusion medium, was stimulated to form myofibers after 5 days of culture in fusion medium containing 50 microM 3-deazaadenosine. Myoblast clone L5/ 3C4...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scarpa S,Strom R,Bozzi A,Aksamit RR,Backlund PS Jr,Chen J,Cantoni GL

更新日期：1984-05-01 00:00:00

abstract：:P elements have been cloned and sequenced from Drosophila nebulosa. Their sequences have diverged less than 6% from P elements of Drosophila melanogaster. However D. nebulosa P elements have nucleotide changes that close all four open reading frames found in the D. melanogaster P element. Microinjection experiments sh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lansman RA,Shade RO,Grigliatti TA,Brock HW

更新日期：1987-09-01 00:00:00

abstract：:The catalytic activity of low-dimensional electrocatalysts is highly dependent on their local atomic structures, particularly those less-coordinated sites found at edges and corners; therefore, a direct probe of the electrocatalytic current at specified local sites with true nanoscopic resolution has become critically...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun T,Wang D,Mirkin MV,Cheng H,Zheng JC,Richards RM,Lin F,Xin HL

更新日期：2019-06-11 00:00:00

abstract：:The productivity of aquatic ecosystems depends on the supply of limiting nutrients. The invasion of the Laurentian Great Lakes, the world's largest freshwater ecosystem, by dreissenid (zebra and quagga) mussels has dramatically altered the ecology of these lakes. A key open question is how dreissenids affect the cycli...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li J,Ianaiev V,Huff A,Zalusky J,Ozersky T,Katsev S

更新日期：2021-02-09 00:00:00

abstract：:Despite the enormous theoretical attention given to the evolutionary consequences of sexual reproduction, the validity of the key assumptions on which the theory depends rarely has been evaluated. It is often argued that a reduced ability to purge deleterious mutations condemns asexual lineages to an early extinction....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Omilian AR,Cristescu ME,Dudycha JL,Lynch M

更新日期：2006-12-05 00:00:00

abstract：:Intrinsically disordered proteins (IDPs) that lack a unique 3D structure and comprise a large fraction of the human proteome play important roles in numerous cellular functions. Prostate-Associated Gene 4 (PAGE4) is an IDP that acts as a potentiator of the Activator Protein-1 (AP-1) transcription factor. Homeodomain-I...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kulkarni P,Jolly MK,Jia D,Mooney SM,Bhargava A,Kagohara LT,Chen Y,Hao P,He Y,Veltri RW,Grishaev A,Weninger K,Levine H,Orban J

更新日期：2017-03-28 00:00:00

abstract：:In Escherichia coli, acetyl phosphate can be formed from acetyl-CoA via the phosphotransacetylase (phosphate acetyltransferase; acetyl-CoA:orthophosphate acetyltransferase, EC 2.3.1.8) reaction and from acetate (plus ATP) via the acetate kinase (ATP:acetate phosphotransferase, EC 2.7.2.1) reaction. By restricting acet...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hong JS,Hunt AG,Masters PS,Lieberman MA

更新日期：1979-03-01 00:00:00

abstract：:Taking advantage of a fluorescent Ca(2+) indicator selectively targeted to the trans-Golgi lumen, we here demonstrate that its Ca(2+) homeostatic mechanisms are distinct from those of the other Golgi subcompartments: (i) Ca(2+) uptake depends exclusively on the activity of the secretory pathway Ca(2+) ATPase1 (SPCA1),...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lissandron V,Podini P,Pizzo P,Pozzan T

更新日期：2010-05-18 00:00:00

abstract：:The Alu element has been a major source of new exons during primate evolution. Thousands of human genes contain spliced exons derived from Alu elements. However, identifying Alu exons that have acquired genuine biological functions remains a major challenge. We investigated the creation and establishment of Alu exons ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shen S,Lin L,Cai JJ,Jiang P,Kenkel EJ,Stroik MR,Sato S,Davidson BL,Xing Y

更新日期：2011-02-15 00:00:00

abstract：:Linear growth delay (stunting) affects roughly 155 million children under the age of 5 years worldwide. Treatment has been limited by a lack of understanding of the underlying pathophysiological mechanisms. Stunting is most likely associated with changes in the microbial community of the small intestine, a compartment...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vonaesch P,Morien E,Andrianonimiadana L,Sanke H,Mbecko JR,Huus KE,Naharimanananirina T,Gondje BP,Nigatoloum SN,Vondo SS,Kaleb Kandou JE,Randremanana R,Rakotondrainipiana M,Mazel F,Djorie SG,Gody JC,Finlay BB,Rubbo PA,

更新日期：2018-09-04 00:00:00