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Heterogeneity of human high density lipoprotein: presence of lipoproteins with and without apoE and their roles as substrates for lecithin:cholesterol acyltransferase reaction.

Abstract：

:By affinity chromatography on heparin-Sepharose, two classes of lipoproteins were separated from high density lipoproteins (HDL) isolated from patients with primary or secondary lecithin:cholesterol acyltransferase (LCATase; EC 2.3.1.43) deficiency and from normal subjects. The unretained fraction, HDL(A), was characterized by having apoA-I as a major apoprotein; it also contained apoA-II, -C-II, and -C-III but it contained only traces of immunodetectable apoE and no apoB. The retained fraction, HDL(E), had apoE as the major apoprotein; it also contained apoA-I, -A-II, -B, -C-II, and -C-III. The relative concentration of apoA-I increased with increasing density in the HDL(E) subclass. Compared to HDL(A), HDL(E) had a significantly higher cholesterol content and a lower protein concentration. HDL(E) was mainly (90%) contained within the HDL(2) subfraction. Contamination of HDL(E) by low density lipoproteins (LDL) or Lp(a) was minimal on the basis of pre-beta-electrophoretic mobility and absence of albumin, respectively. Contamination by LDL or Lp(a) could be resolved in part by application of HDL(E) to concanavalin A-Sepharose or to heparin-Sepharose with a shallow gradient. When evaluated as substrates for a highly purified LCATase preparation, the initial reaction rates and V(max) obtained with HDL(A) were always higher than those obtained with HDL(E) in any given plasma. However, both HDL subclasses from LCATase-deficient subjects were better substrates than the corresponding HDL subclasses from normal plasma. Also, both HDL(3A) and HDL(3E) isolated from normal HDL(3) were better substrates than the corresponding subclasses isolated from normal HDL(2). The recognition of this compositional and functional heterogeneity within HDL will allow a better understanding of the metabolism of this lipoprotein class.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Marcel YL,Vezina C,Emond D,Suzue G

doi

10.1073/pnas.77.5.2969

subject

Has Abstract

pub_date

1980-05-01 00:00:00

pages

2969-73

issue

eissn

0027-8424

issn

1091-6490

journal_volume

pub_type

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Heterogeneity of human high density lipoprotein: presence of lipoproteins with and without apoE and their roles as substrates for lecithin:cholesterol acyltransferase reaction.