Template-directed pausing in in vitro DNA synthesis by DNA polymerase a from Drosophila melanogaster embryos.

abstract：:The unique ability of bovine papillomavirus (BPV-1) DNA to replicate as a stable, multicopy plasmid in transformed mouse cells has led to its utilization as a eukaryotic cloning vector. One limitation of the system has been the marked reduction in transformation efficiency when BPV-1 DNA or the subgenomic transforming...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sarver N,Byrne JC,Howley PM

更新日期：1982-12-01 00:00:00

abstract：:The Cu,Zn superoxide dismutase (SOD) was examined earlier and found to behave in a very unclock-like manner despite (accepted point mutation, or PAM) corrections for multiple replacements per site. Depending upon the time span involved, rates could differ 5-fold. We have sought to determine whether the data might be c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fitch WM,Ayala FJ

更新日期：1994-07-19 00:00:00

abstract：:Peroxiredoxins (Prx), a family of peroxidases that reduce intracellular peroxides with the thioredoxin system as the electron donor, are highly expressed in various cellular compartments. Among the antioxidant Prx enzymes, Prx2 is the most abundant in mammalian neurons, making it a prime candidate to defend against ox...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fang J,Nakamura T,Cho DH,Gu Z,Lipton SA

更新日期：2007-11-20 00:00:00

abstract：:In both randomly moving Dictyostelium and mammalian cells, phosphatidylinositol (3,4,5)-trisphosphate and F-actin are known to propagate as waves at the membrane and act to push out the protruding edge. To date, however, the relationship between the wave geometry and the patterns of amoeboid shape change remains elusi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Taniguchi D,Ishihara S,Oonuki T,Honda-Kitahara M,Kaneko K,Sawai S

更新日期：2013-03-26 00:00:00

abstract：:The primary base sequence of the protein coding regions of the four small heat shock genes of Drosophila melanogaster present at cytological locus 67B has been determined. A single open reading frame large enough to encode a small heat shock protein is found for each gene. The molecular weights of the predicted protei...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ingolia TD,Craig EA

更新日期：1982-04-01 00:00:00

abstract：:Methods to acutely manipulate protein interactions at the subcellular level are powerful tools in cell biology. Several blue-light-dependent optical dimerization tools have been developed. In these systems one protein component of the dimer (the bait) is directed to a specific subcellular location, while the other com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benedetti L,Barentine AES,Messa M,Wheeler H,Bewersdorf J,De Camilli P

更新日期：2018-03-06 00:00:00

abstract：:The antigen recognition of a profoundly immunosuppressive mAb, mAb 2E1, in vivo was investigated. In addition to the 62-kDa effector cell protease receptor 1, mAb 2E1 bound the 32-kDa T cell adhesion receptor E2 (CD99) and the 86-kDa p80 subunit of the nuclear antigen complex Ku. These molecules share no overall seque...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rothermel AL,Altieri DC

更新日期：1998-03-31 00:00:00

abstract：:Germ-line mutations of tumor suppressor genes confer strong predisposition to tumor formation. In the rat, a form of dominantly inherited renal carcinoma (RC) results in multiple chromophobe cell tumors that resemble the human disease, and heterozygous carriers (RC/+) are highly susceptible to environmental agents (ra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yeung RS,Buetow KH,Testa JR,Knudson AG Jr

更新日期：1993-09-01 00:00:00

abstract：:Steroidogenic factor-1 (SF-1) and liver receptor homologue-1 (LRH-1) belong to the fushi tarazu factor 1 subfamily of nuclear receptors. SF-1 is an essential factor for sex determination during development and regulates adrenal and gonadal steroidogenesis in the adult, whereas LRH-1 is a critical factor for developmen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang W,Zhang C,Marimuthu A,Krupka HI,Tabrizizad M,Shelloe R,Mehra U,Eng K,Nguyen H,Settachatgul C,Powell B,Milburn MV,West BL

更新日期：2005-05-24 00:00:00

abstract：:Ellagic acid, a naturally occurring plant phenol, inhibits the activity of the direct-acting mutagen N-methyl-N-nitrosourea (MeNU) in Salmonella typhimurium TA100. Ellagic acid at 0.10, 0.25, 0.50, and 1.00 mM inhibited the mutagenicity of MeNU (0.40 mM) by 3%, 13%, 45%, and 60%, respectively. Ellagic acid (3 mM) also...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dixit R,Gold B

更新日期：1986-11-01 00:00:00

abstract：:Biotrophic plant pathogenic fungi differentiate specialized infection structures within the living cells of their host plants. These haustoria have been linked to nutrient uptake ever since their discovery. We have for the first time to our knowledge shown that the flow of sugars from the host Vicia faba to the rust f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Voegele RT,Struck C,Hahn M,Mendgen K

更新日期：2001-07-03 00:00:00

abstract：:How did language evolve? A popular approach points to the similarities between the ontogeny and phylogeny of language. Young children's language and nonhuman primates' signing both appear formulaic with limited syntactic combinations, thereby suggesting a degree of continuity in their cognitive abilities. To evaluate ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang C

更新日期：2013-04-16 00:00:00

abstract：:The Arabidopsis thaliana AtHKT1 protein, a Na(+)/K(+) transporter, is capable of mediating inward Na(+) currents in Xenopus laevis oocytes and K(+) uptake in Escherichia coli. HKT1 proteins are members of a superfamily of K(+) transporters. These proteins have been proposed to contain eight transmembrane segments and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kato Y,Sakaguchi M,Mori Y,Saito K,Nakamura T,Bakker EP,Sato Y,Goshima S,Uozumi N

更新日期：2001-05-22 00:00:00

abstract：:Cell-based therapies are attractive for revascularizing and regenerating tissues and organs, but clinical trials of endothelial progenitor cell transplantation have not resulted in consistent benefit. We propose a different approach in which a material delivery system is used to create a depot of vascular progenitor c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Silva EA,Kim ES,Kong HJ,Mooney DJ

更新日期：2008-09-23 00:00:00

abstract：:Rotavirus is considered a directly transmitted disease due to its high infectivity. Environmental pathways have, therefore, largely been ignored. Rotavirus, however, persists in water sources, and both its surface water concentrations and infection incidence vary with temperature. Here, we examine the potential for wa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kraay ANM,Brouwer AF,Lin N,Collender PA,Remais JV,Eisenberg JNS

更新日期：2018-03-20 00:00:00

abstract：:Newly translated tubulin molecules undergo a series of complex interactions with nascent chain-binding chaperones, including prefoldin (PFD) and chaperonin-containing TCP-1 (CCT). By screening for oryzalin hypersensitivity, we identified several mutants of Arabidopsis that have lesions in PFD subunits. The pfd6-1 muta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gu Y,Deng Z,Paredez AR,DeBolt S,Wang ZY,Somerville C

更新日期：2008-11-18 00:00:00

abstract：:Emotional signals are crucial for sharing important information, with conspecifics, for example, to warn humans of danger. Humans use a range of different cues to communicate to others how they feel, including facial, vocal, and gestural signals. We examined the recognition of nonverbal emotional vocalizations, such a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sauter DA,Eisner F,Ekman P,Scott SK

更新日期：2010-02-09 00:00:00

abstract：:The neuropeptide galanin is expressed developmentally in the dorsal root ganglion (DRG) and is rapidly up-regulated 120-fold after peripheral nerve section in the adult. Here we report that adult mice carrying a loss-of-function mutation in the galanin gene have a 13% reduction in the number of cells in the DRG associ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holmes FE,Mahoney S,King VR,Bacon A,Kerr NC,Pachnis V,Curtis R,Priestley JV,Wynick D

更新日期：2000-10-10 00:00:00

abstract：:During cardiac trabeculation, cardiomyocytes delaminate from the outermost (compact) layer to form complex muscular structures known as trabeculae. As these cardiomyocytes delaminate, the remodeling of adhesion junctions must be tightly coordinated so cells can extrude from the compact layer while remaining in tight c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cherian AV,Fukuda R,Augustine SM,Maischein HM,Stainier DY

更新日期：2016-07-05 00:00:00

abstract：:Phosphorylation of ADP to ATP was induced in nonrespiring submitochondrial particles (SMP) from rat liver by the application of electric pulses with field strengths of 10-35 kV/cm and a decay time of 60 mus. In all cases respiration was inhibited completely by using cyanide or rotenone. Newly formed ATP was measured b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Teissie J,Knox BE,Tsong TY,Wehrle J

更新日期：1981-12-01 00:00:00

abstract：:The subcellular localization of Ocrl, the inositol polyphosphate 5-phosphatase that is mutated in Lowe syndrome, was investigated by fluorescence microscopy. Ocrl was localized to endosomes and Golgi membranes along with clathrin, giantin, the mannose 6-phosphate receptor, transferrin, and the early endosomal antigen ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ungewickell A,Ward ME,Ungewickell E,Majerus PW

更新日期：2004-09-14 00:00:00

abstract：:Endothelium-derived relaxing factor (EDRF) activity has been attributed to the highly labile nitric oxide radical (NO). In view of the fact that the plasma and cellular milieux contain reactive species that can rapidly inactivate NO, it has been postulated that NO is stabilized by a carrier molecule that preserves its...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stamler JS,Simon DI,Osborne JA,Mullins ME,Jaraki O,Michel T,Singel DJ,Loscalzo J

更新日期：1992-01-01 00:00:00

abstract：:In this note the Weierstrass integral J is taken as L, the Riemann integral of length. Here M(n), n > 1, is a compact, connected manifold of class C(infinity) with a positive definite Riemann structure. Presentations (phi, U(phi)) in M(n) and geodesics (with the aid of the Euler-Riemann equations) are defined in Morse...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morse M

更新日期：1977-06-01 00:00:00

abstract：:In this model gravitation results from the emission and absorption of quanta (gravitons) that are scattered a few times in crossing a typical galaxy. Many features of the universe can be explained in terms of this model, although theoretical justification for the scattering of gravitons is lacking. Gravitons follow a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Britten RJ

更新日期：1992-05-01 00:00:00

abstract：:High-throughput screening (HTS) of chemical compounds to identify modulators of molecular targets is a mainstay of pharmaceutical development. Increasingly, HTS is being used to identify chemical probes of gene, pathway, and cell functions, with the ultimate goal of comprehensively delineating relationships between ch...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Inglese J,Auld DS,Jadhav A,Johnson RL,Simeonov A,Yasgar A,Zheng W,Austin CP

更新日期：2006-08-01 00:00:00

abstract：:The lateral neural plate border (NPB), the neural part of the vertebrate neural border, is composed of central nervous system (CNS) progenitors and peripheral nervous system (PNS) progenitors. In invertebrates, PNS progenitors are also juxtaposed to the lateral boundary of the CNS. Whether there are conserved molecula...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li Y,Zhao D,Horie T,Chen G,Bao H,Chen S,Liu W,Horie R,Liang T,Dong B,Feng Q,Tao Q,Liu X

更新日期：2017-08-01 00:00:00

abstract：:mtDNA mutations occur in a wide variety of degenerative diseases and cancer. mtDNA seems to be more susceptible to DNA damage and consequently sustains higher rates of mutation than does nuclear DNA (nDNA). Many of the somatic mtDNA mutations in human cancers are located in the displacement loop (D-loop) and in partic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mambo E,Gao X,Cohen Y,Guo Z,Talalay P,Sidransky D

更新日期：2003-02-18 00:00:00

abstract：:We have isolated a cDNA clone, called Dmyd for Drosophila myogenic-determination gene, that encodes a protein with structural and functional characteristics similar to the members of the vertebrate MyoD family. Dmyd clone encodes a polypeptide of 332 amino acids with 82% identity to MyoD in the 41 amino acids of the p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Paterson BM,Walldorf U,Eldridge J,Dübendorfer A,Frasch M,Gehring WJ

更新日期：1991-05-01 00:00:00

abstract：:Rat, but not human, erythrocytes undergo fusion promoted by the membrane-mobility agent 2-(2-methoxyethoxy)-ethyl cis-8-(2-octylcyclopropyl)octanoate (A2C). The difference in behavior is correlated with rat erythrocyte membrane protein degradation caused by Ca2+-activated proteases. The human erythrocyte is deficient ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kosower NS,Glaser T,Kosower EM

更新日期：1983-12-01 00:00:00

abstract：:Pathogen exit is a key stage in the spread and propagation of infectious disease, with the fecal-oral route being a common mode of disease transmission. However, it is poorly understood which molecular pathways provide the major modes for intracellular pathogen exit and fecal-oral transmission in vivo. Here, we use th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Szumowski SC,Botts MR,Popovich JJ,Smelkinson MG,Troemel ER

更新日期：2014-06-03 00:00:00