Abstract:
:The neuropeptide galanin is expressed developmentally in the dorsal root ganglion (DRG) and is rapidly up-regulated 120-fold after peripheral nerve section in the adult. Here we report that adult mice carrying a loss-of-function mutation in the galanin gene have a 13% reduction in the number of cells in the DRG associated with a 24% decrease in the percentage of neurons that express substance P. These deficits are associated with a 2.8- and 2.6-fold increase in the number of apoptotic cells in the DRG at postnatal days 3 and 4, respectively. After crush injury to the sciatic nerve, the rate of peripheral nerve regeneration is reduced by 35% with associated long-term functional deficits. Cultured DRG neurons from adult mutant mice demonstrate similar deficits in neurite number and length. These results identify a critical role for galanin in the development and regeneration of sensory neurons.
journal_name
Proc Natl Acad Sci U S Aauthors
Holmes FE,Mahoney S,King VR,Bacon A,Kerr NC,Pachnis V,Curtis R,Priestley JV,Wynick Ddoi
10.1073/pnas.210221897keywords:
subject
Has Abstractpub_date
2000-10-10 00:00:00pages
11563-8issue
21eissn
0027-8424issn
1091-6490pii
210221897journal_volume
97pub_type
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