Elongation of displacement-loop strands in human and mouse mitochondrial DNA is arrested near specific template sequences.

Abstract:

:Animal mitochondrial DNA (mtDNA) maintains a displacement loop (D loop) at the heavy strand origin of replication. These D loops represent sharply limited synthesis of heavy strands and provide a unique opportunity to examine the termination of DNA synthesis. Direct sizing at the nucleotide level indicates that the 3' ends of D-loop strands of human and mouse mtDNA are discrete and map within three to five nucleotides on the complementary template strand. In the case of human mtDNA, there is a single trinucleotide stop point 51-53 nucleotides downstream from a 15-nucleotide template sequence (3'T-A-A-C-C-C-A-A-A-A-A-T-A-C-A 5') which is repeated four times in the mouse mtDNA D-loop region 3'(T-A-A-Py-Py-A-A-A-T-T-A-C-A 5'). The stop points of the five major mouse D-loop strands are 24-63 nucleotides downstream from the four repeated template sequences. These results suggest that the arrest of D-loop strand elongation is an event determined by template sequence.

authors

Doda JN,Wright CT,Clayton DA

doi

10.1073/pnas.78.10.6116

subject

Has Abstract

pub_date

1981-10-01 00:00:00

pages

6116-20

issue

10

eissn

0027-8424

issn

1091-6490

journal_volume

78

pub_type

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