Habituation of tobacco pith cells for factors promoting cell division is heritable and potentially reversible.

abstract：:Electron transfer dissociation (ETD) is a recently introduced mass spectrometric technique that provides a more comprehensive coverage of peptide sequences and posttranslational modifications. Here, we evaluated the use of ETD for a global phosphoproteome analysis. In all, we identified a total of 1,435 phosphorylatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Molina H,Horn DM,Tang N,Mathivanan S,Pandey A

更新日期：2007-02-13 00:00:00

abstract：:The conformation of the pressure-dissociated monomer of Arc repressor was characterized by 1H NMR spectroscopy. The NMR spectra of the monomer under pressure (up to 5.0 kbar; 1 bar = 100 kPa) are typical of a molten globule and they are considerably different from those of the native dimer and thermally denatured mono...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peng X,Jonas J,Silva JL

更新日期：1993-03-01 00:00:00

abstract：:An antiserum was prepared in rabbits against an antigen obtained by density gradient sedimentation of centrifuged medium from monolayer cultures of spleens involved by Hodgkin's disease. The antiserum was tested by isotopic antibody techniques with cells from each of eight cultures derived from spleens involved by Hod...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Long JC,Aisenberg AC,Zamecnik PC

更新日期：1974-07-01 00:00:00

abstract：:A critical step in the induction of apoptosis is the activation of the apoptotic initiator caspase 9. We show that at its normal physiological concentration, caspase 9 is primarily an inactive monomer (zymogen), and that activity is associated with a dimeric species. At the high concentrations used for crystal formati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Renatus M,Stennicke HR,Scott FL,Liddington RC,Salvesen GS

更新日期：2001-12-04 00:00:00

abstract：:The recent observation of superconductivity in [Formula: see text] has raised fundamental questions about the hierarchy of the underlying electronic structure. Calculations suggest that this system falls in the Mott-Hubbard regime, rather than the charge-transfer configuration of other nickel oxides and the supercondu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goodge BH,Li D,Lee K,Osada M,Wang BY,Sawatzky GA,Hwang HY,Kourkoutis LF

更新日期：2021-01-12 00:00:00

abstract：:Galanin is a neuropeptide with a wide variety of biological functions, including that of a strong endogenous anticonvulsant. No nonpeptide ligands, capable of activating galanin receptors, are available today. Based on known pharmacophores of galanin, a combinatorial library was designed, synthesized, and screened at ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Saar K,Mazarati AM,Mahlapuu R,Hallnemo G,Soomets U,Kilk K,Hellberg S,Pooga M,Tolf BR,Shi TS,Hökfelt T,Wasterlain C,Bartfai T,Langel U

更新日期：2002-05-14 00:00:00

abstract：:A method is described in which the interference of radiated second-harmonic electric fields generated by a pair of oriented molecules intercalated into double-stranded DNA is controlled and measured. The results show that the relative molecular orientation of the two molecules significantly changes the magnitude of th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Doughty B,Rao Y,Kazer SW,Kwok SJ,Turro NJ,Eisenthal KB

更新日期：2013-04-09 00:00:00

abstract：:Mass-independent isotope fractionations driven by differences in volumes and shapes of nuclei (the field shift effect) are known in several elements and are likely to be found in more. All-electron relativistic electronic structure calculations can predict this effect but at present are computationally intensive and l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schauble EA

更新日期：2013-10-29 00:00:00

abstract：:To investigate the role of the myosin hinge region in muscle contraction, we examined the contraction characteristics and Mg-ATPase activity of glycerinated muscle fibers prepared from rabbit psoas in the presence and absence of polyclonal antibody directed against the subfragment 2 (S-2) region of myosin. The antibod...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sugi H,Kobayashi T,Gross T,Noguchi K,Karr T,Harrington WF

更新日期：1992-07-01 00:00:00

abstract：:Cellular progenitors of the coronary vasculature are believed to enter the chicken heart during epicardial morphogenesis between stages 17 and 27 (days 3-5) of egg incubation. To trace cells which give rise to the coronary arteries in vivo, we applied retroviral cell tagging procedures and analyzed clonal populations ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mikawa T,Fischman DA

更新日期：1992-10-15 00:00:00

abstract：:One signature of adaptive radiation is a high level of trait change early during the diversification process and a plateau toward the end of the radiation. Although the study of the tempo of evolution has historically been the domain of paleontologists, recently developed phylogenetic tools allow for the rigorous exam...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Agrawal AA,Fishbein M,Halitschke R,Hastings AP,Rabosky DL,Rasmann S

更新日期：2009-10-27 00:00:00

abstract：:In HL-60 cells, a human promyelocytic leukemia cell line, the human c-myc gene, designated MYC, is amplified about 16-fold. On differentiation of the HL-60 cells into granulocytes induced by several inhibitors of poly(ADP-ribose) polymerase [NAD+ poly(adenosine diphosphate D-ribose)ADP-D-ribosyltransferase, EC 2.4.2.3...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shima H,Nakayasu M,Aonuma S,Sugimura T,Nagao M

更新日期：1989-10-01 00:00:00

abstract：:Poly(A)+ mRNA was isolated from the brains of C57BL/6J (B6), DBA/2J (D2), and F1 hybrid mice (B6 X D2) of 16-17 days of age. The yield of polysomal RNA, both poly(A)+ and poly(A)-, from the three strains of mice was comparable. When translated in vitro in a reticulocyte lysate system, the mRNA preparations had the sam...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miskimins R,Ebato H,Seyfried TN,Yu RK

更新日期：1986-03-01 00:00:00

abstract：:Genetic deletion studies have shown that haploinsufficiency of Delta-like ligand (Dll) 4, a transmembrane ligand for the Notch family of receptors, results in major vascular defects and embryonic lethality. To better define the role of Dll4 during vascular growth and differentiation, we selected the postnatal retina a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lobov IB,Renard RA,Papadopoulos N,Gale NW,Thurston G,Yancopoulos GD,Wiegand SJ

更新日期：2007-02-27 00:00:00

abstract：:Determinants of the recommended dietary allowance (RDA) for vitamin C include the relationship between vitamin C dose and steady-state plasma concentration, bioavailability, urinary excretion, cell concentration, and potential adverse effects. Because current data are inadequate, an in-hospital depletion-repletion stu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levine M,Conry-Cantilena C,Wang Y,Welch RW,Washko PW,Dhariwal KR,Park JB,Lazarev A,Graumlich JF,King J,Cantilena LR

更新日期：1996-04-16 00:00:00

abstract：:A somatic mutation in the X linked PIGA gene is responsible for the deficiency of glycosyl phosphatidylinositol (GPI)-anchored proteins on blood cells from patients with paroxysmal nocturnal hemoglobinuria. No inherited form of GPI-anchor deficiency has been described. Because conventional Piga gene knockout is associ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keller P,Tremml G,Rosti V,Bessler M

更新日期：1999-06-22 00:00:00

abstract：:We report on the production of tumor necrosis factor (TNF)-alpha and TNF-beta by mitogen-activated peripheral blood lymphocytes or enriched monocyte subpopulations from human leukocyte antigen (HLA)-typed healthy subjects. The results indicate that HLA-DR2- and DQw1-positive donors frequently exhibit low production of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jacob CO,Fronek Z,Lewis GD,Koo M,Hansen JA,McDevitt HO

更新日期：1990-02-01 00:00:00

abstract：:In translation, elongation factor Tu (EF-Tu) molecules deliver aminoacyl-tRNAs to the mRNA-programmed ribosome. The GTPase activity of EF-Tu is triggered by ribosome-induced conformational changes of the factor that play a pivotal role in the selection of the cognate aminoacyl-tRNAs. We present a 6.7-A cryo-electron m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Villa E,Sengupta J,Trabuco LG,LeBarron J,Baxter WT,Shaikh TR,Grassucci RA,Nissen P,Ehrenberg M,Schulten K,Frank J

更新日期：2009-01-27 00:00:00

abstract：:A readily accessible new class of near infrared (NIR) molecular probes has been synthesized and evaluated. Specific fluorophores in this unique xanthene based regioisomeric seminaphthofluorone dye series exhibit a combination of desirable characteristics including (i) low molecular weight (339 amu), (ii) aqueous solub...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang Y,Lowry M,Xu X,Escobedo JO,Sibrian-Vazquez M,Wong L,Schowalter CM,Jensen TJ,Fronczek FR,Warner IM,Strongin RM

更新日期：2008-07-01 00:00:00

abstract：:Human T cell leukemia virus type 1 (HTLV-1) has evolved a remarkable strategy to thwart the antiviral effects of the cellular cytidine deaminase APOBEC3G (hA3G). HTLV-1 infects T lymphocytes in vivo, where, like HIV-1, it is likely to encounter hA3G. HIV-1 counteracts the innate antiviral activity of hA3G by producing...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Derse D,Hill SA,Princler G,Lloyd P,Heidecker G

更新日期：2007-02-20 00:00:00

abstract：:Mutagenesis of protein-encoding sequences occurs ubiquitously; it enables evolution, accumulates during aging, and is associated with disease. Many biotechnological methods exploit random mutations to evolve novel proteins. To quantitate protein tolerance to random change, it is vital to understand the probability tha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guo HH,Choe J,Loeb LA

更新日期：2004-06-22 00:00:00

abstract：:Adult workers in honeybee (Apis mellifera) colonies exhibit plasticity in hormonally regulated, age-based division of labor by altering their pattern of behavioral development in response to changes in colony conditions. One form of this plasticity is precocious development: levels of juvenile hormone increase prematu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang ZY,Robinson GE

更新日期：1992-12-15 00:00:00

abstract：:We have constructed and characterized a polyoma virus-based plasmid that is maintained as an autonomously replicating extrachromosomal element (episome) in mouse embryonic stem (ES) cells. Plasmid pMGD20neo contains the polyoma origin of replication harboring a mutated enhancer (PyF101), a modified polyoma early regio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gassmann M,Donoho G,Berg P

更新日期：1995-02-28 00:00:00

abstract：:The calcium/calmodulin-dependent protein phosphatase calcineurin stimulates cardiac hypertrophy in response to numerous stimuli. Calcineurin activity is suppressed by association with modulatory calcineurin-interacting protein (MCIP)1DSCR1, which is up-regulated by calcineurin signaling and has been proposed to functi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vega RB,Rothermel BA,Weinheimer CJ,Kovacs A,Naseem RH,Bassel-Duby R,Williams RS,Olson EN

更新日期：2003-01-21 00:00:00

abstract：:In humans, spontaneous movements are often preceded by early brain signals. One such signal is the readiness potential (RP) that gradually arises within the last second preceding a movement. An important question is whether people are able to cancel movements after the elicitation of such RPs, and if so until which po...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schultze-Kraft M,Birman D,Rusconi M,Allefeld C,Görgen K,Dähne S,Blankertz B,Haynes JD

更新日期：2016-01-26 00:00:00

abstract：:In contrast to observations in bacteria and fungi, frameshift mutations in bacteriophage T4 do not arise during genetic recombination. Nascent mutants, captured in the heterozygous condition, exhibit properties which indicate that the new lesions are located at the extreme tips of the chromosomes, and are segregated b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lindstrom DM,Drake JW

更新日期：1970-03-01 00:00:00

abstract：:We model experience-dependent plasticity in the cortical representation of whiskers (the barrel cortex) in normal adult rats, and in adult rats that were prenatally exposed to alcohol. Prenatal exposure to alcohol (PAE) caused marked deficits in experience-dependent plasticity in a cortical barrel-column. Cortical pla...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benusková L,Rema V,Armstrong-James M,Ebner FF

更新日期：2001-02-27 00:00:00

abstract：:A 38-base-pair region required for normal CYC1 mRNA 3' end formation in Saccharomyces cerevisiae was shown to be necessary for the termination of transcription in vivo by examining the stability of CEN3 plasmids. CEN3 plasmids were stably maintained during vegetative growth, unless a GAL1 transcript impinged on the CE...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Russo P,Sherman F

更新日期：1989-11-01 00:00:00

abstract：:The unprecedented scale of the Ebola outbreak in Western Africa (2014-2015) has prompted an explosion of efforts to understand the transmission dynamics of the virus and to analyze the performance of possible containment strategies. Models have focused primarily on the reproductive numbers of the disease that represen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lau MS,Dalziel BD,Funk S,McClelland A,Tiffany A,Riley S,Metcalf CJ,Grenfell BT

更新日期：2017-02-28 00:00:00

abstract：:Coiled-coil motifs are ubiquitous mediators of specific protein-protein interactions through the formation of interlocking hydrophobic seams between alpha-helical chains. Residues that form these seams occur at the first (a) and fourth (d) positions of a characteristic 7-aa repeat and are primarily aliphatic. The pote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu J,Yong W,Deng Y,Kallenbach NR,Lu M

更新日期：2004-11-16 00:00:00