Vitamin C pharmacokinetics in healthy volunteers: evidence for a recommended dietary allowance.

abstract：:The specificity of human skin collagenase and of an enzyme from an invasive tumor were studied by using types I, II, III, IV, and V (AB) collagen as substrates. Human skin collagenase degraded types I, II, and III collagen, producing the characteristic 3/4 and 1/4 cleavage products, but failed to degrade type IV or V ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liotta LA,Abe S,Robey PG,Martin GR

更新日期：1979-05-01 00:00:00

abstract：:We have investigated the theory that the insulin-induced loss of insulin binding from adipocytes is due to internalization of insulin receptors. Cell-surface receptors were assessed by the binding capacity of intact cells at 16 degrees C. Total (i.e., cell-surface plus intracellular) receptors were assessed by solubil...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Green A,Olefsky JM

更新日期：1982-01-01 00:00:00

abstract：:Bicistronic cassettes under control of a single promoter have recently been suggested as useful tools for coordinate expression of two different foreign proteins in mammalian cells. Using the long 5' untranslated region of encephalomyocarditis virus as translational enhancer of the second gene, a bicistronic unit comp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aran JM,Gottesman MM,Pastan I

更新日期：1994-04-12 00:00:00

abstract：:NIH 3T3 mouse cells were transfected with plasmids that induce efficient expression of either (i) the Rous sarcoma virus v-src gene, (ii) the chicken c-src gene, or (iii) a recombinant gene combining the 5' portion of c-src with the 3' end of v-src. Focus formation in tissue culture and formation of large colonies in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shalloway D,Coussens PM,Yaciuk P

更新日期：1984-11-01 00:00:00

abstract：:Reestablishment of vascular homeostasis following ex vivo preservation is a critical determinant of successful organ transplantation. Because the nitric oxide (NO) pathway modulates pulmonary vascular tone and leukocyte/endothelial interactions, we hypothesized that reactive oxygen intermediates would lead to decrease...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pinsky DJ,Naka Y,Chowdhury NC,Liao H,Oz MC,Michler RE,Kubaszewski E,Malinski T,Stern DM

更新日期：1994-12-06 00:00:00

abstract：:The rat brain increases >6x in mass from birth to adulthood, presumably through the addition of glial cells and increasing neuronal size, without the addition of neurons. To test this hypothesis, here we investigate quantitatively the postnatal changes in the total number of neuronal and non-neuronal cells in the deve...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bandeira F,Lent R,Herculano-Houzel S

更新日期：2009-08-18 00:00:00

abstract：:The regional chromosomal location of the human gene for plasminogen activator inhibitor type 1 (PAI1) was determined by three independent methods of gene mapping. PAI1 was localized first to 7cen-q32 and then to 7q21.3-q22 by Southern blot hybridization analysis of a panel of human and mouse somatic cell hybrids with ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Klinger KW,Winqvist R,Riccio A,Andreasen PA,Sartorio R,Nielsen LS,Stuart N,Stanislovitis P,Watkins P,Douglas R

更新日期：1987-12-01 00:00:00

abstract：:In this era of unprecedented biodiversity loss and increased zoonotic disease emergence, it is imperative to understand the effects of biodiversity on zoonotic pathogen dynamics in wildlife. Whether increasing biodiversity should lead to a decrease or increase in infection prevalence, termed the dilution and amplifica...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luis AD,Kuenzi AJ,Mills JN

更新日期：2018-07-31 00:00:00

abstract：:Activation of the mitogen-activated protein kinase (MAP kinase) isoforms ERK1 and ERK2 was investigated in rat adipocytes. Kinase activities were measured by using myelin basic protein as substrate after the isoforms were resolved by Mono Q chromatography or by immunoprecipitation with specific antibodies. Insulin inc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sevetson BR,Kong X,Lawrence JC Jr

更新日期：1993-11-01 00:00:00

abstract：:Broken-cell preparations of Salmonella typhimurium rapidly incorporated 75Se from 75SeO3(2-) into tRNA by an ATP-dependent process. Selenium incorporation in the presence of 50 microM 75SeO3(2-) (0.8-1 pmol per A260 unit) was enhanced by the selenocysteine precursor, O-acetyl-L-serine (to 3.7 pmol per A260 unit). This...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Veres Z,Tsai L,Politino M,Stadtman TC

更新日期：1990-08-01 00:00:00

abstract：:Thyroid cancer is a common endocrine malignancy that encompasses well-differentiated as well as dedifferentiated cancer types. The latter tumors have high mortality and lack effective therapies. Using a paired-end RNA-sequencing approach, we report the discovery of rearrangements involving the anaplastic lymphoma kina...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kelly LM,Barila G,Liu P,Evdokimova VN,Trivedi S,Panebianco F,Gandhi M,Carty SE,Hodak SP,Luo J,Dacic S,Yu YP,Nikiforova MN,Ferris RL,Altschuler DL,Nikiforov YE

更新日期：2014-03-18 00:00:00

abstract：:The second critical endpoint in the basalt-H(2)O system was directly determined by a high-pressure and high-temperature X-ray radiography technique. We found that the second critical endpoint occurs at around 3.4 GPa and 770 °C (corresponding to a depth of approximately 100 km in a subducting slab), which is much shal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mibe K,Kawamoto T,Matsukage KN,Fei Y,Ono S

更新日期：2011-05-17 00:00:00

abstract：:Complex gene regulatory circuits contain many interacting components. In principle, all of these components and interactions may be essential to the function of the circuit. Alternatively, some of them may be refinements to a simpler version of the circuit that improve its fitness. In this work, we have tested whether...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Babić AC,Little JW

更新日期：2007-11-06 00:00:00

abstract：:During cellular stresses, phosphorylation of eukaryotic initiation factor-2 (eIF2) elicits gene expression designed to ameliorate the underlying cellular disturbance. Central to this stress response is the transcriptional regulator activating transcription factor, ATF4. Here we describe the mechanism regulating ATF4 e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vattem KM,Wek RC

更新日期：2004-08-03 00:00:00

abstract：:Two differentiation antigens of spermatogenesis in the mouse are defined by monoclonal antibodies from hybridomas produced between the myeloma P3-X63Ag8 and spleen lymphocytes immunized with syngeneic testis cells. These antigens are on testis cells, and not liver, kidney, brain, spleen, or whole ovary. They are speci...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bechtol KB,Brown SC,Kennett RH

更新日期：1979-01-01 00:00:00

abstract：:The major histocompatibility complex-encoded class I molecule, a noncovalent dimer of a polymorphic 45-kDa heavy chain and a nonpolymorphic 12-kDa beta 2-microglobulin (beta 2m) light chain, binds peptide antigen prior to its interaction with T-cell antigen receptors. We report here that the binding in aqueous solutio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boyd LF,Kozlowski S,Margulies DH

更新日期：1992-03-15 00:00:00

abstract：:The dynamics of social networks can determine the transmission of information, the spread of diseases, and the evolution of behavior. Despite this broad importance, a general framework for predicting social network stability has not been proposed. Here we present longitudinal data on the social dynamics of a cooperati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dakin R,Ryder TB

更新日期：2020-02-11 00:00:00

abstract：:Changes in protein function can lead to changes in the selection acting on specific residues. This can often be detected as evolutionary rate changes at the sites in question. A maximum-likelihood method for detecting evolutionary rate shifts at specific protein positions is presented. The method determines significan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Knudsen B,Miyamoto MM

更新日期：2001-12-04 00:00:00

abstract：:Commercial hydrogen production by electrocatalytic water splitting will benefit from the realization of more efficient and less expensive catalysts compared with noble metal catalysts, especially for the oxygen evolution reaction, which requires a current density of 500 mA/cm2 at an overpotential below 300 mV with lon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhou H,Yu F,Sun J,He R,Chen S,Chu CW,Ren Z

更新日期：2017-05-30 00:00:00

abstract：:A detailed experimental investigation of Fe1+y Te (y = 0.11, 0.12) using pulsed magnetic fields up to 60 T confirms remarkable magnetic shape-memory (MSM) effects. These effects result from magnetoelastic transformation processes in the low-temperature antiferromagnetic state of these materials. The observation of mod...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rößler S,Koz C,Wang Z,Skourski Y,Doerr M,Kasinathan D,Rosner H,Schmidt M,Schwarz U,Rößler UK,Wirth S

更新日期：2019-08-20 00:00:00

abstract：:Coherent multidimensional electronic spectroscopy is commonly used to investigate photophysical phenomena such as light harvesting in photosynthesis in which the system returns back to its ground state after energy transfer. By contrast, we introduce multidimensional spectroscopy to study ultrafast photochemical proce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ruetzel S,Diekmann M,Nuernberger P,Walter C,Engels B,Brixner T

更新日期：2014-04-01 00:00:00

abstract：:We propose that a fundamental problem in the faithful replication of complex chromosomes of higher eukaryotes is the proper control of both the number and timing of the multiple initiations of replication on single chromosomes. When replication patterns are disrupted by any of a variety of agents, overreplication of D...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schimke RT,Sherwood SW,Hill AB,Johnston RN

更新日期：1986-04-01 00:00:00

abstract：:Cell-free extracts of Arbacia eggs (Arbacia punctulata) apparently do not contain an enzymatic system for the reduction of ribonucleotides to deoxyribonucleotides. However, during an interval of 5 hr after fertilization at 23 degrees , an enzymatic system is produced that is capable of catalyzing the reduction of CDP ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Noronha JM,Sheys GH,Buchanan JM

更新日期：1972-08-01 00:00:00

abstract：:Cellular progenitors of the coronary vasculature are believed to enter the chicken heart during epicardial morphogenesis between stages 17 and 27 (days 3-5) of egg incubation. To trace cells which give rise to the coronary arteries in vivo, we applied retroviral cell tagging procedures and analyzed clonal populations ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mikawa T,Fischman DA

更新日期：1992-10-15 00:00:00

abstract：:Decision-making in a natural environment depends on a hierarchy of interacting decision processes. A high-level strategy guides ongoing choices, and the outcomes of those choices determine whether or not the strategy should change. When the right decision strategy is uncertain, as in most natural settings, feedback be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Purcell BA,Kiani R

更新日期：2016-08-02 00:00:00

abstract：:Activated thiol-Sepharose [agarose-(glutathione-2-pyridyl disulfide) conjugate] has been used to immobilize proteins with a single or a few thiol groups via disulfide bridges. The immobilized proteins were subsequently proteolytically degraded. After washing, the thiol-containing peptides were eluted with a reducing a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Egorov TA,Svenson A,Rydén L,Carlsson J

更新日期：1975-08-01 00:00:00

abstract：:NKp46 is a cell surface receptor expressed on natural killer (NK) cells, on a minute subset of T cells, and on a population of innate lymphoid cells that produce IL-22 and express the transcription factor retinoid-related orphan receptor (ROR)-γt, referred to as NK cell receptor (NKR)(+)ROR-γt(+) cells. Here we descri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Narni-Mancinelli E,Chaix J,Fenis A,Kerdiles YM,Yessaad N,Reynders A,Gregoire C,Luche H,Ugolini S,Tomasello E,Walzer T,Vivier E

更新日期：2011-11-08 00:00:00

abstract：:The survival of motor neurons is controlled by multiple factors that regulate different aspects of their physiology. The identification of these factors is important because of their relationship to motor neuron disease. We investigate here whether Mullerian Inhibiting Substance (MIS) is a motor neuron survival factor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang PY,Koishi K,McGeachie AB,Kimber M,Maclaughlin DT,Donahoe PK,McLennan IS

更新日期：2005-11-08 00:00:00

abstract：:We have screened a human DNA library using as probe a chimpanzee sequence that contains homology to the polymerase gene of the endogenous baboon virus. One set of overlapping clones spans about 20 kilobases and contains regions of DNA sequence homology to the gag p30, gag p15, and polymerase genes of Moloney murine le...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bonner TI,O'Connell C,Cohen M

更新日期：1982-08-01 00:00:00

abstract：:It is known from a variety of experimental systems that the ability of tumor cells to grow locally and metastasize can be affected by the presence of adjacent normal tissues and cells, particularly mesenchymally derived stromal cells such as fibroblasts. However, the comparative influence of such normal cell-tumor cel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cornil I,Theodorescu D,Man S,Herlyn M,Jambrosic J,Kerbel RS

更新日期：1991-07-15 00:00:00