当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > A novel functional role for apolipoprotein B in male infertility in heterozygous apolipoprotein B knockout mice.

A novel functional role for apolipoprotein B in male infertility in heterozygous apolipoprotein B knockout mice.

Abstract：

:Male infertility, affecting as many as 10% of the adult population, is an extremely prevalent disorder. In most cases, the cause of the condition is unknown, and genetic factors that might affect male fertility, other than some sequences on the Y chromosome, have not been identified. We report here that male mice heterozygous for a targeted mutation of the apolipoprotein B (apo B) gene exhibit severely compromised fertility. Sperm from these mice failed to fertilize eggs both in vivo and in vitro. However, these sperm were able to fertilize eggs once the zona pellucida was removed but displayed persistent abnormal binding to the egg after fertilization. In vitro fertilization-related and other experiments revealed reduced sperm motility, survival time, and sperm count also contributed to the infertility phenotype. Recognition of the infertility phenotype led to the identification of apo B mRNA in the testes and epididymides of normal mice, and these transcripts were substantially reduced in the affected animals. Moreover, when the genomic sequence encoding human apo B was introduced into these animals, normal fertility was restored. These findings suggest that this genetic locus may have an important impact on male fertility and identify a previously unrecognized function for apo B.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Huang LS,Voyiaziakis E,Chen HL,Rubin EM,Gordon JW

doi

10.1073/pnas.93.20.10903

subject

Has Abstract

pub_date

1996-10-01 00:00:00

pages

10903-7

issue

20

eissn

0027-8424

issn

1091-6490

journal_volume

93

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • TOM1, an Arabidopsis gene required for efficient multiplication of a tobamovirus, encodes a putative transmembrane protein.

    abstract：:Host-encoded factors play an important role in virus multiplication, acting in concert with virus-encoded factors. However, information regarding the host factors involved in this process is limited. Here we report the map-based cloning of an Arabidopsis thaliana gene, TOM1, which is necessary for the efficient multip...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.170295097

    authors： Yamanaka T,Ohta T,Takahashi M,Meshi T,Schmidt R,Dean C,Naito S,Ishikawa M

    更新日期：2000-08-29 00:00:00

  • Genetic control of mammalian T-cell proliferation with synthetic RNA regulatory systems.

    abstract：:RNA molecules perform diverse regulatory functions in natural biological systems, and numerous synthetic RNA-based control devices that integrate sensing and gene-regulatory functions have been demonstrated, predominantly in bacteria and yeast. Despite potential advantages of RNA-based genetic control strategies in cl...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1001721107

    authors： Chen YY,Jensen MC,Smolke CD

    更新日期：2010-05-11 00:00:00

  • A compromise required by gene sharing enables survival: Implications for evolution of new enzyme activities.

    abstract：:Evolution of new enzymatic activities is believed to require a period of gene sharing in which a single enzyme must serve both its original function and a new function that has become advantageous to the organism. Subsequent gene duplication allows one copy to maintain the original function, while the other diverges t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0804804105

    authors： McLoughlin SY,Copley SD

    更新日期：2008-09-09 00:00:00

  • O-acetylation of a cell-surface carbohydrate creates discrete molecular patterns during neural development.

    abstract：:The cell-surface antigen detected by the monoclonal antibody JONES is expressed in the retina and a number of other central nervous system regions of the rat during the latter part of embryonic development and the early postnatal period. In addition to the expression on certain neuroblast populations it is found on so...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.23.8716

    authors： Blum AS,Barnstable CJ

    更新日期：1987-12-01 00:00:00

  • Insect societies as divided organisms: the complexities of purpose and cross-purpose.

    abstract：:Individual organisms are complex in a special way. The organization and function of their parts seem directed toward a purpose: the survival and reproduction of that individual. Groups of organisms are different. They may also be complex, but that is usually because their parts, the individual organisms, are working a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0701285104

    authors： Strassmann JE,Queller DC

    更新日期：2007-05-15 00:00:00

  • Emergence of the Southeast Asian islands as a driver for Neogene cooling.

    abstract：:Steep topography, a tropical climate, and mafic lithologies contribute to efficient chemical weathering and carbon sequestration in the Southeast Asian islands. Ongoing arc-continent collision between the Sunda-Banda arc system and Australia has increased the area of subaerially exposed land in the region since the mi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2011033117

    authors： Park Y,Maffre P,Goddéris Y,Macdonald FA,Anttila ESC,Swanson-Hysell NL

    更新日期：2020-10-13 00:00:00

  • Crystals of hemagglutinin-neuraminidase of parainfluenza virus contain triple-stranded helices.

    abstract：:When purified dimers of hemagglutinin-neuraminidase molecules released by protease digestion from three strains of human parainfluenza virus 1 were used in crystallization trials, long thin needle crystals formed. Electron microscopic analysis of these needle crystals revealed that they are composed of stacks of tripl...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.4.1523

    authors： Murti KG,Takimoto T,Laver WG,Portner A

    更新日期：1993-02-15 00:00:00

  • In situ structure and dynamics of DNA origami determined through molecular dynamics simulations.

    abstract：:The DNA origami method permits folding of long single-stranded DNA into complex 3D structures with subnanometer precision. Transmission electron microscopy, atomic force microscopy, and recently cryo-EM tomography have been used to characterize the properties of such DNA origami objects, however their microscopic stru...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1316521110

    authors： Yoo J,Aksimentiev A

    更新日期：2013-12-10 00:00:00

  • Efficient expression of Escherichia coli galactokinase gene in mammalian cells.

    abstract：:The Escherichia coli galactokinase gene (galK) was inserted into a modified early region transcription unit of simian virus 40 (SV40) contained on a bacterial plasmid. Introduction of this pSVK vector into monkey, mouse, and hamster cell lines by transfection resulted in efficient expression of the bacterial galK gene...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.79.2.257

    authors： Schümperli D,Howard BH,Rosenberg M

    更新日期：1982-01-01 00:00:00

  • Structure of a phleboviral envelope glycoprotein reveals a consolidated model of membrane fusion.

    abstract：:An emergent viral pathogen termed severe fever with thrombocytopenia syndrome virus (SFTSV) is responsible for thousands of clinical cases and associated fatalities in China, Japan, and South Korea. Akin to other phleboviruses, SFTSV relies on a viral glycoprotein, Gc, to catalyze the merger of endosomal host and vira...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1603827113

    authors： Halldorsson S,Behrens AJ,Harlos K,Huiskonen JT,Elliott RM,Crispin M,Brennan B,Bowden TA

    更新日期：2016-06-28 00:00:00

  • Optogenetic activation of dopamine neurons in the ventral tegmental area induces reanimation from general anesthesia.

    abstract：:Dopamine (DA) promotes wakefulness, and DA transporter inhibitors such as dextroamphetamine and methylphenidate are effective for increasing arousal and inducing reanimation, or active emergence from general anesthesia. DA neurons in the ventral tegmental area (VTA) are involved in reward processing, motivation, emoti...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1614340113

    authors： Taylor NE,Van Dort CJ,Kenny JD,Pei J,Guidera JA,Vlasov KY,Lee JT,Boyden ES,Brown EN,Solt K

    更新日期：2016-11-08 00:00:00

  • Biocatalytic synthesis of acrylates in supercritical fluids: tuning enzyme activity by changing pressure.

    abstract：:Supercritical fluids are a unique class of nonaqueous media in which biocatalytic reactions can occur. The physical properties of supercritical fluids, which include gas-like diffusivities and liquid-like densities, can be predictably controlled with changing pressure. This paper describes how adjustment of pressure, ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.7.2940

    authors： Kamat SV,Iwaskewycz B,Beckman EJ,Russell AJ

    更新日期：1993-04-01 00:00:00

  • Identification of a structural domain that distinguishes the actions of the type 1 and 2 isoforms of transforming growth factor beta on endothelial cells.

    abstract：:A chimeric transforming growth factor beta (TGF-beta) molecule has been synthesized to map the amino acids responsible for the substantially greater activity of TGF-beta 1 than TGF-beta 2 on growth and migration of endothelial cells. This chimera consists of a dimer of a monomeric unit composed of amino acids 1-39 of ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.14.6290

    authors： Qian SW,Burmester JK,Merwin JR,Madri JA,Sporn MB,Roberts AB

    更新日期：1992-07-15 00:00:00

  • The transcriptional transactivator of human foamy virus maps to the bel 1 genomic region.

    abstract：:The human foamy virus (HFV) genome possesses three open reading frames (bel 1, 2, and 3) located between env and the 3' long terminal repeat. By analogy to other human retroviruses this region was selected as the most likely candidate to encode the viral transactivator. Results presented here confirmed this and showed...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.3.941

    authors： Rethwilm A,Erlwein O,Baunach G,Maurer B,ter Meulen V

    更新日期：1991-02-01 00:00:00

  • Poly(A) binding protein abundance regulates eukaryotic translation initiation factor 4F assembly in human cytomegalovirus-infected cells.

    abstract：:By commandeering cellular translation initiation factors, or destroying those dispensable for viral mRNA translation, viruses often suppress host protein synthesis. In contrast, cellular protein synthesis proceeds in human cytomegalovirus (HCMV)-infected cells, forcing viral and cellular mRNAs to compete for limiting ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1202829109

    authors： McKinney C,Perez C,Mohr I

    更新日期：2012-04-10 00:00:00

  • Recognition of specific DNA sequences by the c-myb protooncogene product: role of three repeat units in the DNA-binding domain.

    abstract：:The DNA-binding domain of c-Myb consists of three homologous tandem repeats of 52 amino acids. The structure of the third (C-terminal) repeat obtained by NMR analysis has a conformation related to the helix-turn-helix motif. To identify the role of each repeat in the sequence recognition of DNA, we analyzed specific i...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.20.9320

    authors： Tanikawa J,Yasukawa T,Enari M,Ogata K,Nishimura Y,Ishii S,Sarai A

    更新日期：1993-10-15 00:00:00

  • Model for eukaryotic tail-anchored protein binding based on the structure of Get3.

    abstract：:The Get3 ATPase directs the delivery of tail-anchored (TA) proteins to the endoplasmic reticulum (ER). TA-proteins are characterized by having a single transmembrane helix (TM) at their extreme C terminus and include many essential proteins, such as SNAREs, apoptosis factors, and protein translocation components. Thes...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0907522106

    authors： Suloway CJ,Chartron JW,Zaslaver M,Clemons WM Jr

    更新日期：2009-09-01 00:00:00

  • A routine method for protein-free spreading of double- and single-stranded nucleic acid molecules.

    abstract：:A protein-free nucleic acid preparation method for electron microscopy is described. The basic procedure is very similar to the classical protein monolayer spreading techniques. The carrier protein (usually cytochrome c) is replaced by benzyldimethylalkylammonium chloride. Both the hypophase method and the microdiffus...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.72.1.83

    authors： Vollenweider HJ,Sogo JM,Koller T

    更新日期：1975-01-01 00:00:00

  • Fluctuation-enhanced electric conductivity in electrolyte solutions.

    abstract：:We analyze the effects of an externally applied electric field on thermal fluctuations for a binary electrolyte fluid. We show that the fluctuating Poisson-Nernst-Planck (PNP) equations for charged multispecies diffusion coupled with the fluctuating fluid momentum equation result in enhanced charge transport via a mec...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1714464114

    authors： Péraud JP,Nonaka AJ,Bell JB,Donev A,Garcia AL

    更新日期：2017-10-10 00:00:00

  • Single-cell cloning of colon cancer stem cells reveals a multi-lineage differentiation capacity.

    abstract：:Colon carcinoma is one of the leading causes of death from cancer and is characterized by a heterogenic pool of cells with distinct differentiation patterns. Recently, it was reported that a population of undifferentiated cells from a primary tumor, so-called cancer stem cells (CSC), can reconstitute the original tumo...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0805706105

    authors： Vermeulen L,Todaro M,de Sousa Mello F,Sprick MR,Kemper K,Perez Alea M,Richel DJ,Stassi G,Medema JP

    更新日期：2008-09-09 00:00:00

  • Differential lectin binding to cellular membranes in the epidermis of the newborn rat.

    abstract：:Three fluorescein-labeled lectins have been shown to exhibit specificity for the surface of cells in different layers of the epidermis in the newborn rat. An isolectin from seeds of Bandeiraea simplicifolia with specificity for alpha-D-galactosyl end groups labeled the basal and lower spinous cells; a lectin from Ulex...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.77.1.477

    authors： Brabec RK,Peters BP,Bernstein IA,Gray RH,Goldstein IJ

    更新日期：1980-01-01 00:00:00

  • In-drop capillary spooling of spider capture thread inspires hybrid fibers with mixed solid-liquid mechanical properties.

    abstract：:An essential element in the web-trap architecture, the capture silk spun by ecribellate orb spiders consists of glue droplets sitting astride a silk filament. Mechanically this thread presents a mixed solid-liquid behavior unknown to date. Under extension, capture silk behaves as a particularly stretchy solid, owing t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1602451113

    authors： Elettro H,Neukirch S,Vollrath F,Antkowiak A

    更新日期：2016-05-31 00:00:00

  • Hemorrhage in lesions caused by cowpox virus is induced by a viral protein that is related to plasma protein inhibitors of serine proteases.

    abstract：:Several recombinant cowpox viruses were constructed and used to identify a viral gene that controls the production of hemorrhage in lesions caused by the Brighton Red strain of cowpox virus (CPV-BR). This gene is located in the KpnD fragment of CPV-BR DNA, between 31 and 32 kilobases from the end of the genome. This p...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.83.20.7698

    authors： Pickup DJ,Ink BS,Hu W,Ray CA,Joklik WK

    更新日期：1986-10-01 00:00:00

  • Trapped electron cloud bolometer relying on frequency shift.

    abstract：:An improved electron cloud bolometer is analyzed. In this device the cloud temperature is read out not via thermal noise induced by the electrons in a coupled LC circuit but via shift in their axial oscillation frequency in the Penning trap confining them. This shift occurs because as the electron cloud expands with i...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.14.6308

    authors： Dehmelt H

    更新日期：1994-07-05 00:00:00

  • Energy dissipation bounds for autonomous thermodynamic cycles.

    abstract：:How much free energy is irreversibly lost during a thermodynamic process? For deterministic protocols, lower bounds on energy dissipation arise from the thermodynamic friction associated with pushing a system out of equilibrium in finite time. Recent work has also bounded the cost of precisely moving a single degree o...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1915676117

    authors： Bryant SJ,Machta BB

    更新日期：2020-02-18 00:00:00

  • Eosinophil granules function extracellularly as receptor-mediated secretory organelles.

    abstract：:Intracellular granules in several types of leukocytes contain preformed proteins whose secretions contribute to immune and inflammatory functions of leukocytes, including eosinophils, cells notably associated with asthma, allergic inflammation, and helminthic infections. Cytokines and chemokines typically elicit extra...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0804547105

    authors： Neves JS,Perez SA,Spencer LA,Melo RC,Reynolds L,Ghiran I,Mahmudi-Azer S,Odemuyiwa SO,Dvorak AM,Moqbel R,Weller PF

    更新日期：2008-11-25 00:00:00

  • MK2 contributes to tumor progression by promoting M2 macrophage polarization and tumor angiogenesis.

    abstract：:Chronic inflammation is a major risk factor for colorectal cancer. The p38/MAPKAP Kinase 2 (MK2) kinase axis controls the synthesis of proinflammatory cytokines that mediate both chronic inflammation and tumor progression. Blockade of this pathway has been previously reported to suppress inflammation and to prevent co...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1722020115

    authors： Suarez-Lopez L,Sriram G,Kong YW,Morandell S,Merrick KA,Hernandez Y,Haigis KM,Yaffe MB

    更新日期：2018-05-01 00:00:00

  • Ciliary contact interactions dominate surface scattering of swimming eukaryotes.

    abstract：:Interactions between swimming cells and surfaces are essential to many microbiological processes, from bacterial biofilm formation to human fertilization. However, despite their fundamental importance, relatively little is known about the physical mechanisms that govern the scattering of flagellated or ciliated cells ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1210548110

    authors： Kantsler V,Dunkel J,Polin M,Goldstein RE

    更新日期：2013-01-22 00:00:00

  • Regulation of neddylation and deneddylation of cullin1 in SCFSkp2 ubiquitin ligase by F-box protein and substrate.

    abstract：:The activity of cullin-containing ubiquitin protein ligase complexes is stimulated by linkage to cullin of the ubiquitin-like protein Nedd8 ("neddylation"). Neddylation is inhibited by the tight binding of cullins to CAND1 (cullin-associated and neddylation-dissociated 1) protein, and Nedd8 is removed from cullins by ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0603921103

    authors： Bornstein G,Ganoth D,Hershko A

    更新日期：2006-08-01 00:00:00

  • The chemokine SDF-1/CXCL12 modulates the firing pattern of vasopressin neurons and counteracts induced vasopressin release through CXCR4.

    abstract：:Chemokines play a key role in inflammation. They are expressed not only in neuroinflammatory conditions, but also constitutively by different cell types, including neurons in the normal brain, suggesting that they may act as modulators of neuronal functions. Here, we investigated a possible neuroendocrine role of the ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0602620103

    authors： Callewaere C,Banisadr G,Desarménien MG,Mechighel P,Kitabgi P,Rostène WH,Mélik Parsadaniantz S

    更新日期：2006-05-23 00:00:00