Abstract:
:Host-encoded factors play an important role in virus multiplication, acting in concert with virus-encoded factors. However, information regarding the host factors involved in this process is limited. Here we report the map-based cloning of an Arabidopsis thaliana gene, TOM1, which is necessary for the efficient multiplication of tobamoviruses, positive-strand RNA viruses infecting a wide variety of plants. The TOM1 mRNA is suggested to encode a 291-aa polypeptide that is predicted to be a multipass transmembrane protein. The Sos recruitment assay supported the hypothesis that TOM1 is associated with membranes, and in addition, that TOM1 interacts with the helicase domain of tobamovirus-encoded replication proteins. Taken into account that the tobamovirus replication complex is associated with membranes, we propose that TOM1 participates in the in vivo formation of the replication complex by serving as a membrane anchor.
journal_name
Proc Natl Acad Sci U S Aauthors
Yamanaka T,Ohta T,Takahashi M,Meshi T,Schmidt R,Dean C,Naito S,Ishikawa Mdoi
10.1073/pnas.170295097keywords:
subject
Has Abstractpub_date
2000-08-29 00:00:00pages
10107-12issue
18eissn
0027-8424issn
1091-6490pii
170295097journal_volume
97pub_type
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