Abstract:
:The sigma-N (sigmaN) subunit of the bacterial RNA polymerase is a sequence specific DNA-binding protein. The RNA polymerase holoenzyme formed with sigmaN binds to promoters in an inactive form and only initiates transcription when activated by enhancer-binding positive control proteins. We now provide evidence to show that the DNA-binding activity of sigmaN involves two distinct domains: a C-terminal DNA-binding domain that directly contacts DNA and an adjacent domain that enhances DNA-binding activity. The sequences required for the enhancement of DNA binding can be separated from the sequences required for core RNA polymerase binding. These results provide strong evidence for communication between domains within a transcription factor, likely to be important for the function of sigmaN in enhancer-dependent transcription.
journal_name
Proc Natl Acad Sci U S Aauthors
Cannon WV,Chaney MK,Wang X,Buck Mdoi
10.1073/pnas.94.10.5006subject
Has Abstractpub_date
1997-05-13 00:00:00pages
5006-11issue
10eissn
0027-8424issn
1091-6490journal_volume
94pub_type
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