Retroviral analysis of cardiac morphogenesis: discontinuous formation of coronary vessels.

abstract：:The key enzyme for transcription of protein-encoding genes in eukaryotes is RNA polymerase II (RNAPII). The recruitment of this enzyme during transcription initiation and its passage along the template during transcription elongation is regulated through the association and dissociation of several complexes. Elongator...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nelissen H,Fleury D,Bruno L,Robles P,De Veylder L,Traas J,Micol JL,Van Montagu M,Inzé D,Van Lijsebettens M

更新日期：2005-05-24 00:00:00

abstract：:Because of the insolubility and polymeric properties of amyloid fibrils, techniques used conventionally to analyze protein structure and dynamics have often been hampered. Ultrasonication can induce the monomeric solution of amyloidogenic proteins to form amyloid fibrils. However, ultrasonication can break down prefor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chatani E,Lee YH,Yagi H,Yoshimura Y,Naiki H,Goto Y

更新日期：2009-07-07 00:00:00

abstract：:Sialic acid-recognizing Ig-like lectins (Siglecs) are signaling receptors that modulate immune responses, and are targeted for interactions by certain pathogens. We describe two primate Siglecs that were rendered nonfunctional by single genetic events during hominin evolution after our common ancestor with the chimpan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang X,Mitra N,Secundino I,Banda K,Cruz P,Padler-Karavani V,Verhagen A,Reid C,Lari M,Rizzi E,Balsamo C,Corti G,De Bellis G,Longo L,NISC Comparative Sequencing Program.,Beggs W,Caramelli D,Tishkoff SA,Hayakawa T,Gree

更新日期：2012-06-19 00:00:00

abstract：:How can collective action evolve when individuals benefit from cooperation regardless of whether they pay its participation costs? According to one influential perspective, collective action problems are common, especially when groups are large, but may be solved when individuals who have more to gain from the collect...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Langergraber KE,Watts DP,Vigilant L,Mitani JC

更新日期：2017-07-11 00:00:00

abstract：:Perception can be described as a process of inference, integrating bottom-up sensory inputs and top-down expectations. However, it is unclear how this process is neurally implemented. It has been proposed that expectations lead to prestimulus baseline increases in sensory neurons tuned to the expected stimulus, which ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kok P,Mostert P,de Lange FP

更新日期：2017-09-26 00:00:00

abstract：:Von Willebrand factor (vWF) was purified from the plasma of a patient with type IIB von Willebrand disease (vWF from such a patient, IIB vWF) who had a normal platelet count and showed no evidence of spontaneous platelet aggregation. Large multimers of IIB vWF were absent from purified preparations and from plasma. Ri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： De Marco L,Girolami A,Zimmerman TS,Ruggeri ZM

更新日期：1985-11-01 00:00:00

abstract：:The human chaperonin Hsp60 is thought to play a role in the progression of Alzheimer's disease by mitigating against intracellular β-amyloid stress. Here, we show that the bacterial homolog GroEL (51% sequence identity) reduces the neurotoxic effects of amyloid-β(1-42) (Aβ42) on human neural stem cell-derived neuronal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wälti MA,Steiner J,Meng F,Chung HS,Louis JM,Ghirlando R,Tugarinov V,Nath A,Clore GM

更新日期：2018-12-18 00:00:00

abstract：:G-protein-coupled receptors (GPCRs) have recently joined the list of cell surface receptors that dimerize. Dimerization has been shown to alter the ligand-binding, signaling, and trafficking properties of these receptors. Recent studies have shown that GPCRs heterodimerize with closely related members, resulting in th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jordan BA,Trapaidze N,Gomes I,Nivarthi R,Devi LA

更新日期：2001-01-02 00:00:00

abstract：:3-Methylcholanthrene (MCA) skin painting rapidly induced a 100% incidence of thymic lymphomas in RF/J mice. Tumors developed exclusively in cells of the T-lymphocyte lineage and displayed the surface phenotype: Thy-1+, Lyt-1+, Lyt-2+, Qa-1+, H-2K+, H-2D+, TL-, Ig-, Ia-. This phenotype resembled that of cortisone-resis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goodenow MM,Lilly F

更新日期：1984-12-01 00:00:00

abstract：:Synthesis of the 31-amino acid, inner membrane protein MgtS (formerly denoted YneM) is induced by very low Mg2+ in a PhoPQ-dependent manner in Escherichia coli Here we report that MgtS acts to increase intracellular Mg2+ levels and maintain cell integrity upon Mg2+ depletion. Upon development of a functional tagged de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang H,Yin X,Wu Orr M,Dambach M,Curtis R,Storz G

更新日期：2017-05-30 00:00:00

abstract：:Clostridium botulinum neurotoxins (BoNTs), the most potent toxins known, disrupt neurotransmission through proteolysis of proteins involved in neuroexocytosis. The light chains of BoNTs are unique zinc proteases that have stringent substrate specificity and require exceptionally long substrates. We have determined the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Segelke B,Knapp M,Kadkhodayan S,Balhorn R,Rupp B

更新日期：2004-05-04 00:00:00

abstract：:Two molecular forms of pyrophosphate-dependent phosphofructokinase (PP(i)-PFK; pyrophosphate:D-fructose-6-phosphate 1-phosphotransferase; EC 2.7.1.90) have been found whose activity depends upon association and dissociation characteristics regulated by fructose 2,6-bisphosphate (Fru-2,6-P(2)). PP(i)-PFK was purified 2...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu MX,Smyth DA,Black CC

更新日期：1984-08-01 00:00:00

abstract：:Chicken ovalbumin upstream promoter-transcription factor (COUP-TF) genes encode transcription factors belonging to the orphan subfamily of the steroid/thyroid hormone receptor superfamily. Two COUP-TF counterparts have been cloned from mouse. In an attempt to study the function of these genes in the developing central...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Qiu Y,Cooney AJ,Kuratani S,DeMayo FJ,Tsai SY,Tsai MJ

更新日期：1994-05-10 00:00:00

abstract：:Brown-rot fungi such as Postia placenta are common inhabitants of forest ecosystems and are also largely responsible for the destructive decay of wooden structures. Rapid depolymerization of cellulose is a distinguishing feature of brown-rot, but the biochemical mechanisms and underlying genetics are poorly understood...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Martinez D,Challacombe J,Morgenstern I,Hibbett D,Schmoll M,Kubicek CP,Ferreira P,Ruiz-Duenas FJ,Martinez AT,Kersten P,Hammel KE,Vanden Wymelenberg A,Gaskell J,Lindquist E,Sabat G,Bondurant SS,Larrondo LF,Canessa P,Vic

更新日期：2009-02-10 00:00:00

abstract：:The concentration of urea in renal medullary cells is high enough to affect enzymes seriously by reducing Vmax or raising Km, yet the cells survive and function. The usual explanation is that the methylamines found in the renal medulla, namely glycerophosphocholine and betaine, have actions opposite to those of urea a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Burg MB,Peters EM,Bohren KM,Gabbay KH

更新日期：1999-05-25 00:00:00

abstract：:Understanding the genetics of susceptibility to infectious agents is of great importance to our ability to combat disease. Here, we show that voltage-gated calcium channels (VGCCs) are critical for cellular binding and entry of the New World arenaviruses Junín and Tacaribe virus, suggesting that zoonosis via these rec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sarute N,Ross SR

更新日期：2020-08-11 00:00:00

abstract：:A method is proposed for analyzing fast (10 μs) single-molecule rotation trajectories in F1 adenosinetriphosphatase ([Formula: see text]-ATPase). This method is based on the distribution of jumps in the rotation angle that occur in the transitions during the steps between subsequent catalytic dwells. The method is com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Volkán-Kacsó S,Le LQ,Zhu K,Su H,Marcus RA

更新日期：2019-12-17 00:00:00

abstract：:The neuropeptide oxytocin has been implicated in the mediation of several forms of affiliative behavior including parental care, grooming, and sex behavior. Here we demonstrate that species from the genus Microtus (voles) selected for differences in social affiliation show contrasting patterns of oxytocin receptor exp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Insel TR,Shapiro LE

更新日期：1992-07-01 00:00:00

abstract：:Nonesterified fatty acids accumulate at sites of tissue injury and necrosis. In cardiac tissue the concentrations of oleic acid, arachidonic acid, leukotrienes, and other fatty acids increase greatly during ischemia due to receptor or nonreceptor-mediated activation of phospholipases and/or diminished reacylation. In ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang JM,Xian H,Bacaner M

更新日期：1992-07-15 00:00:00

abstract：:The human carcinogen vinyl chloride is metabolized in the liver to reactive intermediates which form N2,3-ethenoguanine in DNA. N2,3-Ethenoguanine is known to cause G----A transitions during DNA replication in Escherichia coli, and its formation may be a carcinogenic event in higher organisms. To investigate the repai...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matijasevic Z,Sekiguchi M,Ludlum DB

更新日期：1992-10-01 00:00:00

abstract：:Acetyl-CoA synthase from Clostridium thermoaceticum (ACS(Ct)) is an alpha(2)beta(2) tetramer containing two novel Ni-X-Fe(4)S(4) active sites (the A and C clusters) and a standard Fe(4)S(4) cluster (the B cluster). The acsA and acsB genes encoding the enzyme were cloned into Escherichia coli strain JM109 and overexpre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Loke HK,Bennett GN,Lindahl PA

更新日期：2000-11-07 00:00:00

abstract：:The cellular replicative DNA polymerases cannot initiate DNA synthesis without a priming 3' OH. During DNA replication, this is supplied in the context of a short RNA primer molecule synthesized by DNA primase. The primase of archaea and eukaryotes, despite having varying subunit compositions, share sequence and struc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yan J,Holzer S,Pellegrini L,Bell SD

更新日期：2018-06-26 00:00:00

abstract：:In feeding, aphids inject saliva into plant tissues, gaining access to phloem sap and eliciting (and sometimes overcoming) plant responses. We are examining the involvement, in this aphid-plant interaction, of individual aphid proteins and enzymes, as identified in a salivary gland cDNA library. Here, we focus on a sa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mutti NS,Louis J,Pappan LK,Pappan K,Begum K,Chen MS,Park Y,Dittmer N,Marshall J,Reese JC,Reeck GR

更新日期：2008-07-22 00:00:00

abstract：:To study C(0)a priori estimates for solutions to certain complex Monge-Ampère equations, I introduce a coherent sheaf of ideals and show that it satisfies various global algebrogeometric conditions, including a cohomology vanishing theorem. This technique is used to establish the existence of Kähler-Einstein metrics o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nadel AM

更新日期：1989-10-01 00:00:00

abstract：:Several nonclassical major histocompatibilty antigens (class Ib molecules) have emerged as key players in the early immune response to pathogens or stress. Class Ib molecules activate subsets of T cells that mount effector responses before the adaptive immune system, and thus are called innate T cells. MR1 is a novel ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang S,Martin E,Kim S,Yu L,Soudais C,Fremont DH,Lantz O,Hansen TH

更新日期：2009-05-19 00:00:00

abstract：:High molecular weight polypeptides (HMWPs) of 270,000 to 340,000 were found to be major components of intermediate filaments prepared by Triton X-100 extraction after spreading of rat glioma C6, HeLa, Chinese hamster ovary, and simian virus 40-transformed Chinese hamster lung cells. C6 HMWPs were shown to resemble hig...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pytela R,Wiche G

更新日期：1980-08-01 00:00:00

abstract：:Several gene fusions have been constructed in which coding sequences for antigenic regions of the pre-S sequences of hepatitis B virus, hepatitis B surface antigen, and the envelope protein of human immunodeficiency virus were linked to the 3' end of that for the first 144 residues of hepatitis B core antigen. The seq...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stahl SJ,Murray K

更新日期：1989-08-01 00:00:00

abstract：:CD31 is an Ig-like molecule expressed by leukocytes and endothelial cells with an established role in the regulation of leukocyte trafficking. Despite genetic deletion of CD31 being associated with exacerbation of T cell-mediated autoimmunity, the contribution of this molecule to T-cell responses is largely unknown. H...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ma L,Mauro C,Cornish GH,Chai JG,Coe D,Fu H,Patton D,Okkenhaug K,Franzoso G,Dyson J,Nourshargh S,Marelli-Berg FM

更新日期：2010-11-09 00:00:00

abstract：:Three components of differentiated muscle membrane, the acetylcholine receptor, acetylcholinesterase (EC 3.1.1.7; acetylcholine hydrolase), and adenylate cyclase [EC 4.6.1.1; ATP pyrophosphate-lyase (cyclizing)], appear simultaneously during myogenesis in cultures of embryonic chick muscle, after the main period of ra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Prives JM,Paterson BM

更新日期：1974-08-01 00:00:00

abstract：:Motion detection is typically spared in blindsight, which results from damage to the striate cortex (area V1) of the brain that is sufficient to eliminate conscious visual awareness and severely reduce sensitivity to luminance contrast, especially for high spatial and low temporal frequencies. Here we show that the di...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Azzopardi P,Hock HS

更新日期：2011-01-11 00:00:00